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The map label for this gene is rho [H]

Identifier: 57238031

GI number: 57238031

Start: 1209129

End: 1210427

Strand: Direct

Name: rho [H]

Synonym: CJE1292

Alternate gene names: 57238031

Gene position: 1209129-1210427 (Clockwise)

Preceding gene: 57238028

Following gene: 57238032

Centisome position: 68.01

GC content: 35.95

Gene sequence:

>1299_bases
ATGGAAAAAGAAAAAAAACAACATCAAAGAACCCACGTTCCAGTGGAAGGTTATAAAATCGAAGAATTAAAATTATTAGA
TTTAGAAAATCTAGTTAAAATCGCCAATGAATGTGAAATCGAAAACCCAAGAGAATTTCGCCGCCAAGAACTTATTTTTG
AAATTTTAAAAGCACAAACAAAAAAAGGCGGTTTTATACTTTTTACAGGGATTTTAGAAATTTCTTCCGAAGGTTATGGC
TTTTTAAGAGGTATGGACTCTAATCTTAGCGATAGTGTTAATGACGCTTATGTTTCAAATTCACAAATTAAAAAATTTGC
TCTGCGTGTAGGCGATATCGTTACAGGACAAGTTAGAGAACCAAAAGATCAAGAAAAATACTACGCTCTTTTAAAAATCG
AAGCGATCAATTATCTGCCTTTACAAGAAGCCAAAGAAAGACCTTTGTTTGATAACTTAACCCCTATTTTTCCAACAGAA
AAAATCAAACTCGAATACGATGCAATGAAGCTTACAGGTCGTGTTTTGGATCTTTTTACTCCTATAGGAAAAGGACAACG
CGGGCTTATCGTTGCTCCACCACGCACAGGTAAAACAGAGCTTATGAAAGAACTTGCTACTGCCATTGCAAAAAACCACC
CAGAAATGCATTTAATCGTACTTTTAGTCGATGAAAGGCCTGAAGAAGTTACCGATATGCAAAGATGCGTAAAAGGGGAG
GTTTTTAGTTCTACTTTTGATTTACCTGCTTATAATCATGTGCGTGTAGCCGAACTCGTGATAGAAAAAGCCAAAAGAAT
GGTAGAAACAGGCAAAGATGTTATTATCTTACTTGATAGTATCACAAGACTGGCACGTGCTTATAACACCGCAACTCCAA
GCAGTGGAAAAGTCCTAAGCGGCGGGGTGGATGCTAATGCCCTACACAAACCAAAACGCTTCTTTGGTGCAGCTAGAAAT
ATAGAAAATGGAGGCTCTTTAACCATAGTTGCTACAGCTTTAATTGATACAGGTTCAAGAATGGATGATGTGATTTTTGA
AGAATTTAAAGGCACAGGAAATAGCGAAATAGTTCTTGATAGAAATATCGCCGATAGAAGAATTTATCCTGCGATTAATA
TCATCAAATCAGGAACAAGAAAAGAAGAATTGCTCCAAGGCGTAGCAAATCTTCAAAAAATTTGGGCAATCCGTTCAGCG
ATTTCTCAAATGGATGATGTTGAAGCGTTGAAATTTTTATATTCTAAAATGCTTAAAACCAAAGACAATGTAGAACTTTT
ATCTATAATGAACGAGTAG

Upstream 100 bases:

>100_bases
AAAATATTTTTTATAATTATAAACAAATTTGACAAAAAGTGCATTTTTTTATTAAAATCACATTTTACAAAAATTATTCT
GCTAAATCTTACGAGGACTT

Downstream 100 bases:

>100_bases
AAAATGCTTCAAGCTTTAGCGATTAAATACAGACCAAAAACCTTTGATGAACTCATAGGACAAAAGACAGTTAGCGTAAG
TTTAAAATACGCCCTAAATC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEKEKKQHQRTHVPVEGYKIEELKLLDLENLVKIANECEIENPREFRRQELIFEILKAQTKKGGFILFTGILEISSEGYG
FLRGMDSNLSDSVNDAYVSNSQIKKFALRVGDIVTGQVREPKDQEKYYALLKIEAINYLPLQEAKERPLFDNLTPIFPTE
KIKLEYDAMKLTGRVLDLFTPIGKGQRGLIVAPPRTGKTELMKELATAIAKNHPEMHLIVLLVDERPEEVTDMQRCVKGE
VFSSTFDLPAYNHVRVAELVIEKAKRMVETGKDVIILLDSITRLARAYNTATPSSGKVLSGGVDANALHKPKRFFGAARN
IENGGSLTIVATALIDTGSRMDDVIFEEFKGTGNSEIVLDRNIADRRIYPAINIIKSGTRKEELLQGVANLQKIWAIRSA
ISQMDDVEALKFLYSKMLKTKDNVELLSIMNE

Sequences:

>Translated_432_residues
MEKEKKQHQRTHVPVEGYKIEELKLLDLENLVKIANECEIENPREFRRQELIFEILKAQTKKGGFILFTGILEISSEGYG
FLRGMDSNLSDSVNDAYVSNSQIKKFALRVGDIVTGQVREPKDQEKYYALLKIEAINYLPLQEAKERPLFDNLTPIFPTE
KIKLEYDAMKLTGRVLDLFTPIGKGQRGLIVAPPRTGKTELMKELATAIAKNHPEMHLIVLLVDERPEEVTDMQRCVKGE
VFSSTFDLPAYNHVRVAELVIEKAKRMVETGKDVIILLDSITRLARAYNTATPSSGKVLSGGVDANALHKPKRFFGAARN
IENGGSLTIVATALIDTGSRMDDVIFEEFKGTGNSEIVLDRNIADRRIYPAINIIKSGTRKEELLQGVANLQKIWAIRSA
ISQMDDVEALKFLYSKMLKTKDNVELLSIMNE
>Mature_432_residues
MEKEKKQHQRTHVPVEGYKIEELKLLDLENLVKIANECEIENPREFRRQELIFEILKAQTKKGGFILFTGILEISSEGYG
FLRGMDSNLSDSVNDAYVSNSQIKKFALRVGDIVTGQVREPKDQEKYYALLKIEAINYLPLQEAKERPLFDNLTPIFPTE
KIKLEYDAMKLTGRVLDLFTPIGKGQRGLIVAPPRTGKTELMKELATAIAKNHPEMHLIVLLVDERPEEVTDMQRCVKGE
VFSSTFDLPAYNHVRVAELVIEKAKRMVETGKDVIILLDSITRLARAYNTATPSSGKVLSGGVDANALHKPKRFFGAARN
IENGGSLTIVATALIDTGSRMDDVIFEEFKGTGNSEIVLDRNIADRRIYPAINIIKSGTRKEELLQGVANLQKIWAIRSA
ISQMDDVEALKFLYSKMLKTKDNVELLSIMNE

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=414, Percent_Identity=56.280193236715, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48669; Mature: 48669

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEKKQHQRTHVPVEGYKIEELKLLDLENLVKIANECEIENPREFRRQELIFEILKAQT
CCCHHHHHHHCCCCCCCEEECCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
KKGGFILFTGILEISSEGYGFLRGMDSNLSDSVNDAYVSNSQIKKFALRVGDIVTGQVRE
CCCCEEEEEEEEHHCCCCCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHCCCEECCCCC
PKDQEKYYALLKIEAINYLPLQEAKERPLFDNLTPIFPTEKIKLEYDAMKLTGRVLDLFT
CCCHHHEEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHC
PIGKGQRGLIVAPPRTGKTELMKELATAIAKNHPEMHLIVLLVDERPEEVTDMQRCVKGE
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCH
VFSSTFDLPAYNHVRVAELVIEKAKRMVETGKDVIILLDSITRLARAYNTATPSSGKVLS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEE
GGVDANALHKPKRFFGAARNIENGGSLTIVATALIDTGSRMDDVIFEEFKGTGNSEIVLD
CCCCCHHHHCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
RNIADRRIYPAINIIKSGTRKEELLQGVANLQKIWAIRSAISQMDDVEALKFLYSKMLKT
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDNVELLSIMNE
CCCHHHHHHHCC
>Mature Secondary Structure
MEKEKKQHQRTHVPVEGYKIEELKLLDLENLVKIANECEIENPREFRRQELIFEILKAQT
CCCHHHHHHHCCCCCCCEEECCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
KKGGFILFTGILEISSEGYGFLRGMDSNLSDSVNDAYVSNSQIKKFALRVGDIVTGQVRE
CCCCEEEEEEEEHHCCCCCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHCCCEECCCCC
PKDQEKYYALLKIEAINYLPLQEAKERPLFDNLTPIFPTEKIKLEYDAMKLTGRVLDLFT
CCCHHHEEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHC
PIGKGQRGLIVAPPRTGKTELMKELATAIAKNHPEMHLIVLLVDERPEEVTDMQRCVKGE
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCH
VFSSTFDLPAYNHVRVAELVIEKAKRMVETGKDVIILLDSITRLARAYNTATPSSGKVLS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEE
GGVDANALHKPKRFFGAARNIENGGSLTIVATALIDTGSRMDDVIFEEFKGTGNSEIVLD
CCCCCHHHHCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
RNIADRRIYPAINIIKSGTRKEELLQGVANLQKIWAIRSAISQMDDVEALKFLYSKMLKT
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDNVELLSIMNE
CCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]