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The map label for this gene is lepA [H]

Identifier: 57237917

GI number: 57237917

Start: 1089816

End: 1091612

Strand: Reverse

Name: lepA [H]

Synonym: CJE1174

Alternate gene names: 57237917

Gene position: 1091612-1089816 (Counterclockwise)

Preceding gene: 57237920

Following gene: 57237916

Centisome position: 61.4

GC content: 33.39

Gene sequence:

>1797_bases
TTGTCGGTAAAAAATATTAGAAATTTTTCTATTATAGCTCATATAGATCATGGAAAATCTACTCTTGCAGATAGGATTAT
TAGTGAGTGTGGTGCTATTAGCGATAGGCAGATGAGTTCACAAGTTATGGATACTATGGATATAGAAAAAGAGCGTGGTA
TCACCATAAAAGCACAATCAGTGCGTTTAAATTATAAATTTAATAATGAAAATTTTGTTTTAAATCTTATTGATACTCCT
GGCCATGTGGATTTTTCTTATGAAGTGAGTCGTTCTTTGGCAAGTTGTGAAGGAGCTTTGCTTGTAGTAGATGCTTCTCA
AGGTGTTGAAGCACAGACTATAGCTAATGTTTATATAGCGCTTGAAAATAATCTTGAAATCATTCCTGTGATTAATAAAA
TCGATTTACCTAATGCTGATGTTGAGAAAGTAAAGCATGAAATCGAGCATATTATAGGTATAGATTGCAAAGATGCAATT
TGTGTGAGTGCAAAAACAGGAGTGGGTATAAAAGAGCTTATAGAAACAATTATTACTAAAATCCCTGCACCAAAAACAGA
TGATGAAGCTCCTACTAAGGCTTTAATTTATGATTCTTGGTTTGATAATTATTTGGGTGCTTTGGCTTTGGTTAGGATTT
ATGAAGGAAGTATTGCTAAAAACGATGAAGTTTTAGTCATGAGTACGGATAAAAAACATATAGTTCAAGATCTTTTTTAT
CCCCACCCTTTAAGTCCAATTAAAACTCAATCTTTACAATCAGGCGAAGTAGGTGTGGTGGTTTTAGGGCTTAAAACCGT
AGGTGATGTACAAGTAGGCGATACCATCACTTTGGTAAAAAACAAAGCTAAAGAAGCCATAGGTGGTTTTGAAAAGGCTA
AAGCTTTTGTATTTGCGGGACTTTATCCTATAGAAACGGATAAATTTGAAGATTTAAGAGATGCTTTGGATAAATTAAAG
CTTAATGATAGTTCTATTACTTATGAGCCTGAAACTTCTTTGGCCTTAGGCTTTGGTTTTAGGGTAGGATTTTTAGGGCT
TTTACATATGGAAGTTATCAAAGAAAGGCTTGAGAGAGAATTTAATCTTGATTTGATTGCTACAGCTCCAACTGTGACTT
ATGAAATTTATCAAACCGATGGAGAGTTTATTAAAATTCAAAATCCTAGCGAGCTTCCTCCTGTTAATAAAATCGATCAT
ATAAAAGAGCCTTATGTTAAAGCTACTATCATTACTCCAAGTGAATTTTTAGGGAATTTAATCACTCTTTTAAATCGCAA
AAGAGGAGTACAGGTTAAAATGGACTATATCACACCTGAGCGTGTTTTGCTAGAATATGATGTGCCTTTAAATGAGATTG
TGATGGATTTTTATGATAAATTAAAGTCCTTAACAAAAGGTTATGCGAGTTTTGATTATGAGCCTATAGAATTTAGAGTA
GGGGATTTGGTAAAACTTGATATTAAGGTAGCGGGTGAAAATGTTGATGCGCTAAGCATTATCGTGCCTAATGAAAAAGC
GCAAAGTAAAGGAAGAGAACTTGTTAGTGCTATGAAAGAAATAGTTCCTAGACAGCTTTTTGAAGTGGCCATTCAAGCAA
GTATTGGAAATAAAATCATCGCAAGAGAAACTGTTAAATCCATGGGTAAAAATGTGACAGCAAAATGTTATGGTGGGGAT
ATTACTAGAAAAAGAAAGCTTTTAGAAAAGCAAAAAGAAGGTAAAAAAAGAATGAAAGCTATAGGTAAGGTAAATTTACC
TCAAGAGGCTTTTTTGAGTGTTTTAAAGATAGATTGA

Upstream 100 bases:

>100_bases
AATTTCAATTGAAGTTTTTTATATAAAATAAAATTTATTTGTTTTAAACATAAATTTTGTTAAAATCCTAGCTTTAAAAT
TTTATATTTAGGATGAAATT

Downstream 100 bases:

>100_bases
TGATAAATTTAAAACTTGGCTTGAAATTTGCTTGTATTTTTAATACAATTTTAAAATAATTTTCTAAGGAGAAAAAATGT
TTAAAAAATTTTTGATTTTT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSVKNIRNFSIIAHIDHGKSTLADRIISECGAISDRQMSSQVMDTMDIEKERGITIKAQSVRLNYKFNNENFVLNLIDTP
GHVDFSYEVSRSLASCEGALLVVDASQGVEAQTIANVYIALENNLEIIPVINKIDLPNADVEKVKHEIEHIIGIDCKDAI
CVSAKTGVGIKELIETIITKIPAPKTDDEAPTKALIYDSWFDNYLGALALVRIYEGSIAKNDEVLVMSTDKKHIVQDLFY
PHPLSPIKTQSLQSGEVGVVVLGLKTVGDVQVGDTITLVKNKAKEAIGGFEKAKAFVFAGLYPIETDKFEDLRDALDKLK
LNDSSITYEPETSLALGFGFRVGFLGLLHMEVIKERLEREFNLDLIATAPTVTYEIYQTDGEFIKIQNPSELPPVNKIDH
IKEPYVKATIITPSEFLGNLITLLNRKRGVQVKMDYITPERVLLEYDVPLNEIVMDFYDKLKSLTKGYASFDYEPIEFRV
GDLVKLDIKVAGENVDALSIIVPNEKAQSKGRELVSAMKEIVPRQLFEVAIQASIGNKIIARETVKSMGKNVTAKCYGGD
ITRKRKLLEKQKEGKKRMKAIGKVNLPQEAFLSVLKID

Sequences:

>Translated_598_residues
MSVKNIRNFSIIAHIDHGKSTLADRIISECGAISDRQMSSQVMDTMDIEKERGITIKAQSVRLNYKFNNENFVLNLIDTP
GHVDFSYEVSRSLASCEGALLVVDASQGVEAQTIANVYIALENNLEIIPVINKIDLPNADVEKVKHEIEHIIGIDCKDAI
CVSAKTGVGIKELIETIITKIPAPKTDDEAPTKALIYDSWFDNYLGALALVRIYEGSIAKNDEVLVMSTDKKHIVQDLFY
PHPLSPIKTQSLQSGEVGVVVLGLKTVGDVQVGDTITLVKNKAKEAIGGFEKAKAFVFAGLYPIETDKFEDLRDALDKLK
LNDSSITYEPETSLALGFGFRVGFLGLLHMEVIKERLEREFNLDLIATAPTVTYEIYQTDGEFIKIQNPSELPPVNKIDH
IKEPYVKATIITPSEFLGNLITLLNRKRGVQVKMDYITPERVLLEYDVPLNEIVMDFYDKLKSLTKGYASFDYEPIEFRV
GDLVKLDIKVAGENVDALSIIVPNEKAQSKGRELVSAMKEIVPRQLFEVAIQASIGNKIIARETVKSMGKNVTAKCYGGD
ITRKRKLLEKQKEGKKRMKAIGKVNLPQEAFLSVLKID
>Mature_597_residues
SVKNIRNFSIIAHIDHGKSTLADRIISECGAISDRQMSSQVMDTMDIEKERGITIKAQSVRLNYKFNNENFVLNLIDTPG
HVDFSYEVSRSLASCEGALLVVDASQGVEAQTIANVYIALENNLEIIPVINKIDLPNADVEKVKHEIEHIIGIDCKDAIC
VSAKTGVGIKELIETIITKIPAPKTDDEAPTKALIYDSWFDNYLGALALVRIYEGSIAKNDEVLVMSTDKKHIVQDLFYP
HPLSPIKTQSLQSGEVGVVVLGLKTVGDVQVGDTITLVKNKAKEAIGGFEKAKAFVFAGLYPIETDKFEDLRDALDKLKL
NDSSITYEPETSLALGFGFRVGFLGLLHMEVIKERLEREFNLDLIATAPTVTYEIYQTDGEFIKIQNPSELPPVNKIDHI
KEPYVKATIITPSEFLGNLITLLNRKRGVQVKMDYITPERVLLEYDVPLNEIVMDFYDKLKSLTKGYASFDYEPIEFRVG
DLVKLDIKVAGENVDALSIIVPNEKAQSKGRELVSAMKEIVPRQLFEVAIQASIGNKIIARETVKSMGKNVTAKCYGGDI
TRKRKLLEKQKEGKKRMKAIGKVNLPQEAFLSVLKID

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=49.3355481727575, Blast_Score=605, Evalue=1e-173,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=49.2424242424242, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=46.7889908256881, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=46.7889908256881, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=46.7889908256881, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=42.1052631578947, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=42.1052631578947, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=42.1052631578947, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI18390331, Length=155, Percent_Identity=36.7741935483871, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI217272892, Length=160, Percent_Identity=30.625, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI217272894, Length=160, Percent_Identity=30.625, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI94966752, Length=131, Percent_Identity=33.587786259542, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI53729339, Length=222, Percent_Identity=28.3783783783784, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI53729337, Length=222, Percent_Identity=28.3783783783784, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=58.3892617449664, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=503, Percent_Identity=27.6341948310139, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1790835, Length=157, Percent_Identity=31.8471337579618, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=35.2201257861635, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789559, Length=295, Percent_Identity=27.4576271186441, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789108, Length=152, Percent_Identity=30.2631578947368, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=41.0801963993453, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=36.9426751592357, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=170, Percent_Identity=37.0588235294118, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=170, Percent_Identity=37.0588235294118, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=152, Percent_Identity=37.5, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=139, Percent_Identity=38.1294964028777, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=158, Percent_Identity=31.0126582278481, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=608, Percent_Identity=46.7105263157895, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=43.1506849315069, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=43.1506849315069, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=141, Percent_Identity=38.2978723404255, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=111, Percent_Identity=40.5405405405405, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=38.2978723404255, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=236, Percent_Identity=25.8474576271186, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=44.205298013245, Blast_Score=544, Evalue=1e-155,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=43.4782608695652, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=38.9261744966443, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=38.9261744966443, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=38.9261744966443, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582462, Length=139, Percent_Identity=39.568345323741, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI221458488, Length=142, Percent_Identity=40.1408450704225, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=34.5679012345679, Blast_Score=90, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28572034, Length=232, Percent_Identity=29.7413793103448, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921738, Length=281, Percent_Identity=29.5373665480427, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66579; Mature: 66448

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKNIRNFSIIAHIDHGKSTLADRIISECGAISDRQMSSQVMDTMDIEKERGITIKAQS
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEEEE
VRLNYKFNNENFVLNLIDTPGHVDFSYEVSRSLASCEGALLVVDASQGVEAQTIANVYIA
EEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCHHEEEEEEEE
LENNLEIIPVINKIDLPNADVEKVKHEIEHIIGIDCKDAICVSAKTGVGIKELIETIITK
EECCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHH
IPAPKTDDEAPTKALIYDSWFDNYLGALALVRIYEGSIAKNDEVLVMSTDKKHIVQDLFY
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCC
PHPLSPIKTQSLQSGEVGVVVLGLKTVGDVQVGDTITLVKNKAKEAIGGFEKAKAFVFAG
CCCCCCCCCCCCCCCCEEEEEEEEEECCCEECCCEEEEHHHHHHHHHCCHHHHCEEEEEC
LYPIETDKFEDLRDALDKLKLNDSSITYEPETSLALGFGFRVGFLGLLHMEVIKERLERE
CEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
FNLDLIATAPTVTYEIYQTDGEFIKIQNPSELPPVNKIDHIKEPYVKATIITPSEFLGNL
CCCEEEEECCEEEEEEEECCCCEEEECCCCCCCCCCCHHHCCCCCEEEEEECHHHHHHHH
ITLLNRKRGVQVKMDYITPERVLLEYDVPLNEIVMDFYDKLKSLTKGYASFDYEPIEFRV
HHHHCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GDLVKLDIKVAGENVDALSIIVPNEKAQSKGRELVSAMKEIVPRQLFEVAIQASIGNKII
CCEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ARETVKSMGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVNLPQEAFLSVLKID
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SVKNIRNFSIIAHIDHGKSTLADRIISECGAISDRQMSSQVMDTMDIEKERGITIKAQS
CCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEEEE
VRLNYKFNNENFVLNLIDTPGHVDFSYEVSRSLASCEGALLVVDASQGVEAQTIANVYIA
EEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCHHEEEEEEEE
LENNLEIIPVINKIDLPNADVEKVKHEIEHIIGIDCKDAICVSAKTGVGIKELIETIITK
EECCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHH
IPAPKTDDEAPTKALIYDSWFDNYLGALALVRIYEGSIAKNDEVLVMSTDKKHIVQDLFY
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCC
PHPLSPIKTQSLQSGEVGVVVLGLKTVGDVQVGDTITLVKNKAKEAIGGFEKAKAFVFAG
CCCCCCCCCCCCCCCCEEEEEEEEEECCCEECCCEEEEHHHHHHHHHCCHHHHCEEEEEC
LYPIETDKFEDLRDALDKLKLNDSSITYEPETSLALGFGFRVGFLGLLHMEVIKERLERE
CEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
FNLDLIATAPTVTYEIYQTDGEFIKIQNPSELPPVNKIDHIKEPYVKATIITPSEFLGNL
CCCEEEEECCEEEEEEEECCCCEEEECCCCCCCCCCCHHHCCCCCEEEEEECHHHHHHHH
ITLLNRKRGVQVKMDYITPERVLLEYDVPLNEIVMDFYDKLKSLTKGYASFDYEPIEFRV
HHHHCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GDLVKLDIKVAGENVDALSIIVPNEKAQSKGRELVSAMKEIVPRQLFEVAIQASIGNKII
CCEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ARETVKSMGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVNLPQEAFLSVLKID
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA