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The map label for this gene is metG [H]

Identifier: 57237679

GI number: 57237679

Start: 863978

End: 865864

Strand: Reverse

Name: metG [H]

Synonym: CJE0925

Alternate gene names: 57237679

Gene position: 865864-863978 (Counterclockwise)

Preceding gene: 57237680

Following gene: 57237678

Centisome position: 48.7

GC content: 31.37

Gene sequence:

>1887_bases
ATGCGTTATATTACAACACCAATTTATTATGTAAATGATGTGCCTCATTTAGGTCATGCTTATACTACCATCATAGCCGA
TACTTTAGCAAGATTTTATCGTTTGCAAGGACATGAAACAAGATTTCTAACAGGAACAGATGAGCATGGACAAAAAATCG
AAGAAGCTGCCAAACTAAGAAATTCCACTCCTCAAGAATACGCTGATAAAATCAGTTTTGAATTTAAAAAATTATGGGAT
GAATTTGAAATCACCTATGATATTTACGCAAGAACAACTGATACAAGGCATATTGAATTTGTTAAAGCTATGTTTTTAAA
AATGTGGCAAAAAGGTGATATTTATAAAGATGAATACGAAGGACACTATTGTATTTCTTGTGAAAGCTTTTTTACTCAAA
GTCAGCTTATCAATGATTGTTCTTGTCCAGATTGTGGCAAACAAACTAGAATTTTAAAAGAAGAAAGCTATTTTTTCAAA
CTTTCAAAATATCAAGATAAAATTTTACAATGGTATGAAGAAAAAGATCCCATCTTGCCAAAAAACAAAAAAAACGAACT
TATAAATTTTGTCCAAAACGGCCTTAAAGATCTTTCCATTACAAGAACAAGTTTTGATTGGGGGATTAAACTGCCTCAAG
AAATTAATGATGATAAACATATTGTTTATGTATGGCTTGATGCACTTTTTATCTATGTAAGCTCACTTGATTTTCAAAGC
AAGGGAGAAAATACTAAATTTTGGCCTGCGCATGTGCATTTAGTAGGAAAAGATATCTTGCGTTTTCATGCCATTTATTG
GCCAGCTTTTTTAATGAGCGTAGATTTACCTTTGCCAAAATTTATAGGTGCACATGGCTGGTGGACTAAAGAAGGTGAAA
AAATGAGCAAATCTAAAGGCAATGTTGTAAAACCTAAAGAAGTGGTTGATGCTTATGGAAGCGAAGCTTTTAGATATTTC
TTGCTTAGAGAAGTACCTTTTGGTAATGATGGGGATTTTAGCGAAAATATGCTGATAAATCGTATCAATGCAGAGCTTAG
CAATGAATTTGGAAATTTACTTAATCGCATTATAGGTATGAGTACAAAATATTCTCAAGGAAATATTTCAAAAGAAGGTG
TTTTAAAATTTTACAATGCCGAACTAAATCAAGCAAAAGAACACTTAAATTTAGCGGTAGAATTTTTAGAAAACTTACAG
TGTAATCGTTATTTAGAAGAACTTTTTAAAGCCTTAAGTGTGGCAAATTTAGCCATTAGTAAATACGAACCTTGGAATTT
AATCAAAGAAAACAAACACGAACAAGCTAATGCTTTAGTAGCACTTTGTGCAAATATTTTAGCCAAAACAAGCCTTTTAT
TAAGCCCTACTCTACCAAAGTCTTGTCAAAAGGTGGCCTTAGCTTTAAATTTTGAAATTTCATCTACAAATTATGCAAAA
ATGATACTTGATAATAAACTTTTAGATTTTAAAGCAAATCCTTGCGAAGCTTTATTTCCAAAAGTAGAAAAAGCTCTTTT
AAAACAAGAAATTAAAGAAGAACCTAAAAAAGAAGAAAGTCCTAAAATAAAAATCGATGATTTTGCAAAAATTGAAATCA
AAGTTGCTAAGGTGCTTGATTGTCAAAATATAAAAGGAAGCGAAAAACTTTTGAAATTTCAACTCGAACTTGACGATAAA
GAAATCCGTCAAGTACTCTCAGGCATAGCAAAACACTATAAAGCCAGTGATTTAATCGGTAAACAAGTTTGCATTATAAG
CAATCTTAAAAAAGCTAAAATTTTTGGACATGAAAGCGATGGTATGATACTTTCTGCAAAAAGCGGAGATAAGCTTGTTT
TAATCACCCCTGAACAACTTGTACAAAACGGTTCTTTAGTGGGTTAA

Upstream 100 bases:

>100_bases
AATTTTTAACTGCCAAACTAGAAAAAATTATGTTGATGCAGTCTATAACAGTATGTCTAAGGGTAAAACCGAAGGCGGAT
TTGACTTTTAAAGGAAAAAA

Downstream 100 bases:

>100_bases
AATATGCAAATTAGCAATTTAGGCGAACTTTTAAACGCGATACTCATTCATGAAGGTAGCGTTTTAAGCGCGGAAGGTTT
TGCCATAAATTTAAACGAAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MRYITTPIYYVNDVPHLGHAYTTIIADTLARFYRLQGHETRFLTGTDEHGQKIEEAAKLRNSTPQEYADKISFEFKKLWD
EFEITYDIYARTTDTRHIEFVKAMFLKMWQKGDIYKDEYEGHYCISCESFFTQSQLINDCSCPDCGKQTRILKEESYFFK
LSKYQDKILQWYEEKDPILPKNKKNELINFVQNGLKDLSITRTSFDWGIKLPQEINDDKHIVYVWLDALFIYVSSLDFQS
KGENTKFWPAHVHLVGKDILRFHAIYWPAFLMSVDLPLPKFIGAHGWWTKEGEKMSKSKGNVVKPKEVVDAYGSEAFRYF
LLREVPFGNDGDFSENMLINRINAELSNEFGNLLNRIIGMSTKYSQGNISKEGVLKFYNAELNQAKEHLNLAVEFLENLQ
CNRYLEELFKALSVANLAISKYEPWNLIKENKHEQANALVALCANILAKTSLLLSPTLPKSCQKVALALNFEISSTNYAK
MILDNKLLDFKANPCEALFPKVEKALLKQEIKEEPKKEESPKIKIDDFAKIEIKVAKVLDCQNIKGSEKLLKFQLELDDK
EIRQVLSGIAKHYKASDLIGKQVCIISNLKKAKIFGHESDGMILSAKSGDKLVLITPEQLVQNGSLVG

Sequences:

>Translated_628_residues
MRYITTPIYYVNDVPHLGHAYTTIIADTLARFYRLQGHETRFLTGTDEHGQKIEEAAKLRNSTPQEYADKISFEFKKLWD
EFEITYDIYARTTDTRHIEFVKAMFLKMWQKGDIYKDEYEGHYCISCESFFTQSQLINDCSCPDCGKQTRILKEESYFFK
LSKYQDKILQWYEEKDPILPKNKKNELINFVQNGLKDLSITRTSFDWGIKLPQEINDDKHIVYVWLDALFIYVSSLDFQS
KGENTKFWPAHVHLVGKDILRFHAIYWPAFLMSVDLPLPKFIGAHGWWTKEGEKMSKSKGNVVKPKEVVDAYGSEAFRYF
LLREVPFGNDGDFSENMLINRINAELSNEFGNLLNRIIGMSTKYSQGNISKEGVLKFYNAELNQAKEHLNLAVEFLENLQ
CNRYLEELFKALSVANLAISKYEPWNLIKENKHEQANALVALCANILAKTSLLLSPTLPKSCQKVALALNFEISSTNYAK
MILDNKLLDFKANPCEALFPKVEKALLKQEIKEEPKKEESPKIKIDDFAKIEIKVAKVLDCQNIKGSEKLLKFQLELDDK
EIRQVLSGIAKHYKASDLIGKQVCIISNLKKAKIFGHESDGMILSAKSGDKLVLITPEQLVQNGSLVG
>Mature_628_residues
MRYITTPIYYVNDVPHLGHAYTTIIADTLARFYRLQGHETRFLTGTDEHGQKIEEAAKLRNSTPQEYADKISFEFKKLWD
EFEITYDIYARTTDTRHIEFVKAMFLKMWQKGDIYKDEYEGHYCISCESFFTQSQLINDCSCPDCGKQTRILKEESYFFK
LSKYQDKILQWYEEKDPILPKNKKNELINFVQNGLKDLSITRTSFDWGIKLPQEINDDKHIVYVWLDALFIYVSSLDFQS
KGENTKFWPAHVHLVGKDILRFHAIYWPAFLMSVDLPLPKFIGAHGWWTKEGEKMSKSKGNVVKPKEVVDAYGSEAFRYF
LLREVPFGNDGDFSENMLINRINAELSNEFGNLLNRIIGMSTKYSQGNISKEGVLKFYNAELNQAKEHLNLAVEFLENLQ
CNRYLEELFKALSVANLAISKYEPWNLIKENKHEQANALVALCANILAKTSLLLSPTLPKSCQKVALALNFEISSTNYAK
MILDNKLLDFKANPCEALFPKVEKALLKQEIKEEPKKEESPKIKIDDFAKIEIKVAKVLDCQNIKGSEKLLKFQLELDDK
EIRQVLSGIAKHYKASDLIGKQVCIISNLKKAKIFGHESDGMILSAKSGDKLVLITPEQLVQNGSLVG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=545, Percent_Identity=32.1100917431193, Blast_Score=279, Evalue=6e-75,
Organism=Homo sapiens, GI14043022, Length=532, Percent_Identity=26.6917293233083, Blast_Score=166, Evalue=6e-41,
Organism=Escherichia coli, GI1788432, Length=684, Percent_Identity=27.046783625731, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1789455, Length=100, Percent_Identity=33, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=486, Percent_Identity=31.6872427983539, Blast_Score=263, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71992781, Length=381, Percent_Identity=29.1338582677165, Blast_Score=170, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17540794, Length=505, Percent_Identity=27.5247524752475, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=32.2935779816514, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321704, Length=510, Percent_Identity=26.8627450980392, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358483, Length=548, Percent_Identity=32.1167883211679, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24655311, Length=525, Percent_Identity=26.8571428571429, Blast_Score=175, Evalue=7e-44,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 72245; Mature: 72245

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYITTPIYYVNDVPHLGHAYTTIIADTLARFYRLQGHETRFLTGTDEHGQKIEEAAKLR
CCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
NSTPQEYADKISFEFKKLWDEFEITYDIYARTTDTRHIEFVKAMFLKMWQKGDIYKDEYE
CCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GHYCISCESFFTQSQLINDCSCPDCGKQTRILKEESYFFKLSKYQDKILQWYEEKDPILP
CEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCC
KNKKNELINFVQNGLKDLSITRTSFDWGIKLPQEINDDKHIVYVWLDALFIYVSSLDFQS
CCCHHHHHHHHHHCHHHCEEEEECCCCCCCCHHHCCCCCEEEEHHHHHHHHHHHCCCCCC
KGENTKFWPAHVHLVGKDILRFHAIYWPAFLMSVDLPLPKFIGAHGWWTKEGEKMSKSKG
CCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHCCC
NVVKPKEVVDAYGSEAFRYFLLREVPFGNDGDFSENMLINRINAELSNEFGNLLNRIIGM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
STKYSQGNISKEGVLKFYNAELNQAKEHLNLAVEFLENLQCNRYLEELFKALSVANLAIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
KYEPWNLIKENKHEQANALVALCANILAKTSLLLSPTLPKSCQKVALALNFEISSTNYAK
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEEEEEECCCCHHH
MILDNKLLDFKANPCEALFPKVEKALLKQEIKEEPKKEESPKIKIDDFAKIEIKVAKVLD
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEEEEHHC
CQNIKGSEKLLKFQLELDDKEIRQVLSGIAKHYKASDLIGKQVCIISNLKKAKIFGHESD
CCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHEECCCCC
GMILSAKSGDKLVLITPEQLVQNGSLVG
CEEEEECCCCEEEEECHHHHHCCCCCCC
>Mature Secondary Structure
MRYITTPIYYVNDVPHLGHAYTTIIADTLARFYRLQGHETRFLTGTDEHGQKIEEAAKLR
CCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
NSTPQEYADKISFEFKKLWDEFEITYDIYARTTDTRHIEFVKAMFLKMWQKGDIYKDEYE
CCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GHYCISCESFFTQSQLINDCSCPDCGKQTRILKEESYFFKLSKYQDKILQWYEEKDPILP
CEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCC
KNKKNELINFVQNGLKDLSITRTSFDWGIKLPQEINDDKHIVYVWLDALFIYVSSLDFQS
CCCHHHHHHHHHHCHHHCEEEEECCCCCCCCHHHCCCCCEEEEHHHHHHHHHHHCCCCCC
KGENTKFWPAHVHLVGKDILRFHAIYWPAFLMSVDLPLPKFIGAHGWWTKEGEKMSKSKG
CCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHCCC
NVVKPKEVVDAYGSEAFRYFLLREVPFGNDGDFSENMLINRINAELSNEFGNLLNRIIGM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
STKYSQGNISKEGVLKFYNAELNQAKEHLNLAVEFLENLQCNRYLEELFKALSVANLAIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
KYEPWNLIKENKHEQANALVALCANILAKTSLLLSPTLPKSCQKVALALNFEISSTNYAK
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEEEEEECCCCHHH
MILDNKLLDFKANPCEALFPKVEKALLKQEIKEEPKKEESPKIKIDDFAKIEIKVAKVLD
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEEEEHHC
CQNIKGSEKLLKFQLELDDKEIRQVLSGIAKHYKASDLIGKQVCIISNLKKAKIFGHESD
CCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHEECCCCC
GMILSAKSGDKLVLITPEQLVQNGSLVG
CEEEEECCCCEEEEECHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]