Click here to switch to the map view.

The map label for this gene is acnB [H]

Identifier: 57237676

GI number: 57237676

Start: 859988

End: 862534

Strand: Reverse

Name: acnB [H]

Synonym: CJE0922

Alternate gene names: 57237676

Gene position: 862534-859988 (Counterclockwise)

Preceding gene: 57237678

Following gene: 57237675

Centisome position: 48.52

GC content: 36.32

Gene sequence:

>2547_bases
ATGTCTTTTATACAAGAATACAATAAACTTGTTGAAGAAAGAGCCGCTTTGGGTATACCACCCCTTCCTTTAAATGCAAA
TCAAACTAGAGAACTTTGTAAATTACTTGAAAATGAAAATAATGAAGAGCTTGCAAATCTTTTAGAAAATAGAGTAAATC
CTGGTGTTGATGATGCAGCTTTAGTAAAATGTGAATTTTTAGATTCCATACTTAAAGGCAAAATCAGTGCTCCAAATATA
GACAAAAAAAGAGCCTTAAGAATGCTTGGAACTATGCTAGGCGGATATAATGTTAAAGTTTTAATCGATGCCTTAAAAGA
TGAAAACATAGCCAAAGATGCTGCAGAAGTGTTGAAAAATATTATTTTTGTACATGATAATTTCCATACCATAGCTGAAC
TTAGCAAAAACAATCCACACGCTAAGGAAGTTTTACAAAGCTGGGCAAATGCGGACTGGTTTAATAAAAAAGAAAAACTA
CCACAAGTGATTAAATGTATAGTTTTTAAAGTAGCAGGGGAAACAAATACCGATGATCTAAGCCCTGCAGGAGATGCTTT
TACAAGAAGCGATATTCCTTTACATGCAAATGCCATGCTAAAGGTAAGACAAACAGGATCCTTAGAAAAAATCAAAGAGC
TTAAAAAAAGCGGTCGCGAAGTGGTTTATGTAGGTGATGTTGTAGGAACAGGTTCAAGTAGAAAATCGGCAATAAATTCC
ATACAATGGCACTTAGGAAAAGAAATAGAAGGTGTGCCAAACAAGCACAGCGGTGGTATAGTAATGGGTTCAACCATAGC
ACCGATTTTCTTTAACACTGCTCAAGATAGCGGGGCTTTACCTATCATCTGTGATGTAACAAACTTAGAAATGGGCGATG
AGTTTGAAATTCATCCTTATGATGGAAAAATCATAAAAAATGGCTCTGCAATCGCAGAATTTACACTCAGTCCAAACACT
CTCTTAGATGAAGTTAGAGCAGGAGGAAGAATCCCACTTATCATAGGTCGTGGGCTTTGTACTAAGGCTAGAGAATTTTT
AGGAATGGAAAGCGAAAATATCTTTACCAAACCTGAACAACCTAAATCTTCAAGCGGTGGTTATACCTTGGCTCAAAAAA
TGCTAGGTCGTGCCTGTGGAGTTGAAGGTGTGCGTCCAGGTATGTATATAGAACCTATAACTTTAACCGTAGGCTCTCAA
GATACCACAGGTCCTATGACTAGAGATGAAATTAAAGAACTTGCAAGCCTAGGTTTTAATGCAGACTTTGTTATGCAAAG
CTTTTGCCATACAGCTGCGTATCCAAAAGTAAGCGATTCAAATTTACATAAAACCTTGCCCAATTTCATGACAAGTCGTG
GTGGGGTAAGTTTAAAACCAGGTGATGGAGTTATCCACTCTTGGCTTAATCGTTTTGTATTGCCAGATACCGTAGGAACG
GGTGGGGATTCTCACACACGCTTTCCTATAGGAATTTCTTTCCCTGCAGGAAGCGGACTTGTAGCTTTTGCTGCAGTTAC
TGGTTCTATGCCTTTAAATGTCCCTGAAAGTGTTTTAGTACGTTTTAGTGGAGAATTGCAAGCCGGAGTTACCCTAAGAG
ATTTGGTTAATGCTATACCTTATTATGCAATCAAACAAGGACAACTTACCGTTGAAAAGAAAAATAAGAAAAATATTTTT
GCAGGAAAAATTTTAGAAATAGAAGGCTTGCCAAACTTAAAAGTCGAACAAGCTTTTGAACTTAGTGATGCAAGTGCTGA
AAGATCTGCAGCTGCTTGTAGTGTAGATTTAAGCATAGAAAGTGTAAGCGAATATATCAAATCCAATATTTCTTTAATTG
AAGCTATGATAGAAGCAGGATATGAGAATAAAGCTACCCTTGCTAGAAGGGCTGAAAAAATGAGAGAATGGCTTAAAAAC
CCTACTCTTTTAAGAGCAGATAAAGATGCAAAATACGCTTATATTATAGATATTAATCTTAATGACATTAAAGAGCCAAT
CCTAGCTTGTCCAAATGACCCTGATGATGTGGCAACCTTAAGCGAAATTTTAGCCGATAATAAGCGTCCAAAAAATATAG
ATGAAGTTTTTGTAGGTTCTTGTATGACAAACATAGGACATTATAGAGCTTTGGGAGAAATTTTAAAAGATAAAGGCATA
CTTAAAACCAGACTTTGGGTAGTGCCTCCAACAAAAATGGATAAAGCACAACTTATTAATGAAGGTTATTACAGCATCTT
TGGCGCAGCAGGTGCTAGAATAGAAGTTCCTGGTTGTTCTTTGTGTATGGGTAATCAAGCTAGAGTAAATGATGGAGCTG
TAGTTTTCTCAACTTCTACAAGAAATTTTGACAACCGTATGGGAATGGGTGCTAAAGTATATTTAGGCAGTGCAGAACTT
GCGGCAGTTTGTGCGATTTTGGGTAAAATTCCAAGCAAGGAAGAATACTTACAAATTGTTAGCGAAAAACTAAGTGATGA
GCATAAGGCAAATATCTATAGATATTTAAATTTTAATGAGATTGAAAATTTCAAACTTGAAAATTAA

Upstream 100 bases:

>100_bases
GTAAAGATAGGTAACATAAAGGCATTTTATAATAAACTTTAAACATTTTTTATCCTTCAAAGAGCTACAATTATAATAAA
AAATATTTAAGGAGTAGAAA

Downstream 100 bases:

>100_bases
TTCAAATTTAATACTTATTTGGATTAAATAACAAAATTAAAATTTTCAAAGGAAAAATATTGAAGTTATTATTCGCTTCC
TTTTGTTGTTTTGCAAGTTT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 848; Mature: 847

Protein sequence:

>848_residues
MSFIQEYNKLVEERAALGIPPLPLNANQTRELCKLLENENNEELANLLENRVNPGVDDAALVKCEFLDSILKGKISAPNI
DKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKNIIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKL
PQVIKCIVFKVAGETNTDDLSPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSGREVVYVGDVVGTGSSRKSAINS
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPYDGKIIKNGSAIAEFTLSPNT
LLDEVRAGGRIPLIIGRGLCTKAREFLGMESENIFTKPEQPKSSSGGYTLAQKMLGRACGVEGVRPGMYIEPITLTVGSQ
DTTGPMTRDEIKELASLGFNADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDTVGT
GGDSHTRFPIGISFPAGSGLVAFAAVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIPYYAIKQGQLTVEKKNKKNIF
AGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIESVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKN
PTLLRADKDAKYAYIIDINLNDIKEPILACPNDPDDVATLSEILADNKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
LKTRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTSTRNFDNRMGMGAKVYLGSAEL
AAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNEIENFKLEN

Sequences:

>Translated_848_residues
MSFIQEYNKLVEERAALGIPPLPLNANQTRELCKLLENENNEELANLLENRVNPGVDDAALVKCEFLDSILKGKISAPNI
DKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKNIIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKL
PQVIKCIVFKVAGETNTDDLSPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSGREVVYVGDVVGTGSSRKSAINS
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPYDGKIIKNGSAIAEFTLSPNT
LLDEVRAGGRIPLIIGRGLCTKAREFLGMESENIFTKPEQPKSSSGGYTLAQKMLGRACGVEGVRPGMYIEPITLTVGSQ
DTTGPMTRDEIKELASLGFNADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDTVGT
GGDSHTRFPIGISFPAGSGLVAFAAVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIPYYAIKQGQLTVEKKNKKNIF
AGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIESVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKN
PTLLRADKDAKYAYIIDINLNDIKEPILACPNDPDDVATLSEILADNKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
LKTRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTSTRNFDNRMGMGAKVYLGSAEL
AAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNEIENFKLEN
>Mature_847_residues
SFIQEYNKLVEERAALGIPPLPLNANQTRELCKLLENENNEELANLLENRVNPGVDDAALVKCEFLDSILKGKISAPNID
KKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKNIIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKLP
QVIKCIVFKVAGETNTDDLSPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSGREVVYVGDVVGTGSSRKSAINSI
QWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPYDGKIIKNGSAIAEFTLSPNTL
LDEVRAGGRIPLIIGRGLCTKAREFLGMESENIFTKPEQPKSSSGGYTLAQKMLGRACGVEGVRPGMYIEPITLTVGSQD
TTGPMTRDEIKELASLGFNADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDTVGTG
GDSHTRFPIGISFPAGSGLVAFAAVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIPYYAIKQGQLTVEKKNKKNIFA
GKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIESVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKNP
TLLRADKDAKYAYIIDINLNDIKEPILACPNDPDDVATLSEILADNKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGIL
KTRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTSTRNFDNRMGMGAKVYLGSAELA
AVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNEIENFKLEN

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=859, Percent_Identity=60.6519208381839, Blast_Score=1019, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=371, Percent_Identity=26.1455525606469, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323335, Length=389, Percent_Identity=24.9357326478149, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=23.6040609137056, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=23.6040609137056, Blast_Score=67, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=23.6040609137056, Blast_Score=67, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92691; Mature: 92560

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIQEYNKLVEERAALGIPPLPLNANQTRELCKLLENENNEELANLLENRVNPGVDDAA
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
LVKCEFLDSILKGKISAPNIDKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKN
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHH
IIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKLPQVIKCIVFKVAGETNTDDL
HEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSGREVVYVGDVVGTGSSRKSAINS
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECEECCCCCHHHHHHH
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPY
HHHHCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCEEEEECC
DGKIIKNGSAIAEFTLSPNTLLDEVRAGGRIPLIIGRGLCTKAREFLGMESENIFTKPEQ
CCEEEECCCEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCC
PKSSSGGYTLAQKMLGRACGVEGVRPGMYIEPITLTVGSQDTTGPMTRDEIKELASLGFN
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCC
ADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDTVGT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCCCC
GGDSHTRFPIGISFPAGSGLVAFAAVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIP
CCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHCC
YYAIKQGQLTVEKKNKKNIFAGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIE
CEEEECCEEEEECCCCCCEEECEEEEECCCCCCCHHHHHCCCCCCHHHCCCEEEEECCHH
SVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKNPTLLRADKDAKYAYIIDINL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECH
NDIKEPILACPNDPDDVATLSEILADNKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LKTRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTST
EEEEEEEECCCCCCHHHHHHCCHHHHHCCCCCEEECCCCCEECCCCCEECCCEEEEEECC
RNFDNRMGMGAKVYLGSAELAAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNE
CCCHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEECCCC
IENFKLEN
CCCCCCCC
>Mature Secondary Structure 
SFIQEYNKLVEERAALGIPPLPLNANQTRELCKLLENENNEELANLLENRVNPGVDDAA
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
LVKCEFLDSILKGKISAPNIDKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKN
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHH
IIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKLPQVIKCIVFKVAGETNTDDL
HEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSGREVVYVGDVVGTGSSRKSAINS
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECEECCCCCHHHHHHH
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPY
HHHHCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCEEEEECC
DGKIIKNGSAIAEFTLSPNTLLDEVRAGGRIPLIIGRGLCTKAREFLGMESENIFTKPEQ
CCEEEECCCEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCC
PKSSSGGYTLAQKMLGRACGVEGVRPGMYIEPITLTVGSQDTTGPMTRDEIKELASLGFN
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCC
ADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDTVGT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCCCC
GGDSHTRFPIGISFPAGSGLVAFAAVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIP
CCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHCC
YYAIKQGQLTVEKKNKKNIFAGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIE
CEEEECCEEEEECCCCCCEEECEEEEECCCCCCCHHHHHCCCCCCHHHCCCEEEEECCHH
SVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKNPTLLRADKDAKYAYIIDINL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECH
NDIKEPILACPNDPDDVATLSEILADNKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LKTRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTST
EEEEEEEECCCCCCHHHHHHCCHHHHHCCCCCEEECCCCCEECCCCCEECCCEEEEEECC
RNFDNRMGMGAKVYLGSAELAAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNE
CCCHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEECCCC
IENFKLEN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]