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The map label for this gene is recG [H]

Identifier: 57237518

GI number: 57237518

Start: 466911

End: 468734

Strand: Direct

Name: recG [H]

Synonym: CJE0514

Alternate gene names: 57237518

Gene position: 466911-468734 (Clockwise)

Preceding gene: 57237517

Following gene: 57237520

Centisome position: 26.26

GC content: 29.71

Gene sequence:

>1824_bases
ATGAAAATTAAAGAAAGTGATTTTGAATTTTTTAAAAAATTAAAAATTAAAAGTGCTATTGATTTAGCGCTTCTTTTACC
TAAAAAAATTGAAAATTTAAATCCAAGTAAAAATCCTAAAGAAAATGAAATTTGCACCCAAAAAATCACTATAAAAAGCG
TTAGTTCAAGAAAAAATCAACTTTTTGGTTTAGGTTTTTGTGAAGAATGGCAAGAAAATATTTCTTTTGTGTTTTTTCAT
CCTAGAGCTTGGCATTTTGGGATTTGTAAGGTAGGAAAAGGGCTTATTTTTAATGCTAAACTCTCGCGTTTTAATCACAC
TTGGCAATTTAACAATCCTAAAATTTTAACTTCTTTTGAGGGTTTTAGTCCAAAGTATCAAATTTTAGGTTTAAAAGATG
CAAAAATTGCTGCTTTTATACATAAATATCTTAATTATGAAAATTTACAAGAAAGTGGCATAGGGGATAAATATATACAT
TTTCTTTTAAATTTGCATGCTTATGATGAAAAAAGTTTTTTTATGTTTGAAAATTTGCAAAATTTTAGCAAAGATTTAAA
ATATATAGAAATATATAATTTTTTAAAGCGTTTAAAAGCTAAAAAAACACATTTTAAAGCTCATCAAATCAATGTTTTTA
ACATAGCTAATTGGCTTAAAGATTTGCCTTTTTCTCTTACTAAAGATCAGCTTAATGCCTTAAAAGATATAGAAAAAGAT
TTGCATTCAAAAGAAGCTAGAAGGCGTGTAATTATGGGTGATGTAGGGTGTGGTAAAACCTTGGTTTTGCTTGGTGCGGC
TTTGATGGTTTATCCTAAACAAGCTATTTTGATGGCACCTACTAGTATTTTAGCGCATCAGCTTTATGAAGAGGCTAAGA
AATTTTTGCCTGATTTTATGAATATTTTGTTTATTAAAGGGGGTAAAAAAGAAAAAGATTTAGAACAAAATATTCAAAAA
GCAAATCTTATTATAGGTACTCATGCTTTAATTCATTTAGAAAGCCATAATGCCGTACTTGTAATGATAGATGAACAACA
TCGTTTCGGCTCGGCTCAGCGTGAGAAAATTTATTCTTTAAGTAAACAAGAATTTGCCCCTCATTTTATACAGTTTTCGG
CTACGCCTATTCCAAGAACCTTAAGCATGATACAATCTGAACTTTTAAATTTTAGTTTCATTAAACAAATGCCTTTTAAA
AAAGATATCACAACTTATTGTATACAAAATGAAGACTTTTCTAAGCTTAGCGAGAAGATTAAAGAAGAAATCGCTAAAAA
TCATCAAATTATCATTATTTATCCCTTAGTGAGTGCTAGTGATAATATTCCTTATCTTTCTTTAGAGCAGGCTAAAGAAT
ATTGGCAGAGTCACTATGAAAAAGTTTTTGTAACTCATGGGAAAGATAAACAAAAAGATGAAATTTTAGAGCGTTTTAGA
GATGAGGGAAATATTTTACTAAGCACTACAGTGGTGGAAGTGGGCATTTCTTTGCCAAGATTGAGTATGATAGTGATTGT
AGGAGCGGAACGTTTAGGACTTGCTACCTTGCATCAACTTCGTGGTCGTGTAGGTCGTGTAGGGCTTAAAAGCACTTGTT
ATCTTTATACTAAACTTAAAGAAATTCCAAGTCGTTTAAAAGAATTTGCTAGCACTTTAGATGGGTTTAAAATAGCAGAG
CTTGATCTTAAAAACCGTTTGAGTGGAGATTTGCTCGATGGTTTTATGCAACATGGAAATGAGTTTAAATTTTTTGATTT
TTCTAAGGATGAGGAGATTTTAGAAAAGGTAAAAAAAGATTTGGCAAAAAAAATGCCAAATTAA

Upstream 100 bases:

>100_bases
GAAGAGTTAAAACTTATGGAAAAGGTTAAACTTGAAACCATCAATGCTTATATTAACAAACATCAAGAGCTTTTAAATAT
AAGTTTTGCAAGCATACAAA

Downstream 100 bases:

>100_bases
TGTCCACCATACTTATAAAGCATATTTTGAAAATGTGAAGCTATCATTTGTGCACGCTGTAAAATAACTGTTTTCATTTC
TTCATTATAAACTATATTTA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MKIKESDFEFFKKLKIKSAIDLALLLPKKIENLNPSKNPKENEICTQKITIKSVSSRKNQLFGLGFCEEWQENISFVFFH
PRAWHFGICKVGKGLIFNAKLSRFNHTWQFNNPKILTSFEGFSPKYQILGLKDAKIAAFIHKYLNYENLQESGIGDKYIH
FLLNLHAYDEKSFFMFENLQNFSKDLKYIEIYNFLKRLKAKKTHFKAHQINVFNIANWLKDLPFSLTKDQLNALKDIEKD
LHSKEARRRVIMGDVGCGKTLVLLGAALMVYPKQAILMAPTSILAHQLYEEAKKFLPDFMNILFIKGGKKEKDLEQNIQK
ANLIIGTHALIHLESHNAVLVMIDEQHRFGSAQREKIYSLSKQEFAPHFIQFSATPIPRTLSMIQSELLNFSFIKQMPFK
KDITTYCIQNEDFSKLSEKIKEEIAKNHQIIIIYPLVSASDNIPYLSLEQAKEYWQSHYEKVFVTHGKDKQKDEILERFR
DEGNILLSTTVVEVGISLPRLSMIVIVGAERLGLATLHQLRGRVGRVGLKSTCYLYTKLKEIPSRLKEFASTLDGFKIAE
LDLKNRLSGDLLDGFMQHGNEFKFFDFSKDEEILEKVKKDLAKKMPN

Sequences:

>Translated_607_residues
MKIKESDFEFFKKLKIKSAIDLALLLPKKIENLNPSKNPKENEICTQKITIKSVSSRKNQLFGLGFCEEWQENISFVFFH
PRAWHFGICKVGKGLIFNAKLSRFNHTWQFNNPKILTSFEGFSPKYQILGLKDAKIAAFIHKYLNYENLQESGIGDKYIH
FLLNLHAYDEKSFFMFENLQNFSKDLKYIEIYNFLKRLKAKKTHFKAHQINVFNIANWLKDLPFSLTKDQLNALKDIEKD
LHSKEARRRVIMGDVGCGKTLVLLGAALMVYPKQAILMAPTSILAHQLYEEAKKFLPDFMNILFIKGGKKEKDLEQNIQK
ANLIIGTHALIHLESHNAVLVMIDEQHRFGSAQREKIYSLSKQEFAPHFIQFSATPIPRTLSMIQSELLNFSFIKQMPFK
KDITTYCIQNEDFSKLSEKIKEEIAKNHQIIIIYPLVSASDNIPYLSLEQAKEYWQSHYEKVFVTHGKDKQKDEILERFR
DEGNILLSTTVVEVGISLPRLSMIVIVGAERLGLATLHQLRGRVGRVGLKSTCYLYTKLKEIPSRLKEFASTLDGFKIAE
LDLKNRLSGDLLDGFMQHGNEFKFFDFSKDEEILEKVKKDLAKKMPN
>Mature_607_residues
MKIKESDFEFFKKLKIKSAIDLALLLPKKIENLNPSKNPKENEICTQKITIKSVSSRKNQLFGLGFCEEWQENISFVFFH
PRAWHFGICKVGKGLIFNAKLSRFNHTWQFNNPKILTSFEGFSPKYQILGLKDAKIAAFIHKYLNYENLQESGIGDKYIH
FLLNLHAYDEKSFFMFENLQNFSKDLKYIEIYNFLKRLKAKKTHFKAHQINVFNIANWLKDLPFSLTKDQLNALKDIEKD
LHSKEARRRVIMGDVGCGKTLVLLGAALMVYPKQAILMAPTSILAHQLYEEAKKFLPDFMNILFIKGGKKEKDLEQNIQK
ANLIIGTHALIHLESHNAVLVMIDEQHRFGSAQREKIYSLSKQEFAPHFIQFSATPIPRTLSMIQSELLNFSFIKQMPFK
KDITTYCIQNEDFSKLSEKIKEEIAKNHQIIIIYPLVSASDNIPYLSLEQAKEYWQSHYEKVFVTHGKDKQKDEILERFR
DEGNILLSTTVVEVGISLPRLSMIVIVGAERLGLATLHQLRGRVGRVGLKSTCYLYTKLKEIPSRLKEFASTLDGFKIAE
LDLKNRLSGDLLDGFMQHGNEFKFFDFSKDEEILEKVKKDLAKKMPN

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=429, Percent_Identity=34.7319347319347, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1787357, Length=396, Percent_Identity=30.5555555555556, Blast_Score=155, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 70265; Mature: 70265

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKESDFEFFKKLKIKSAIDLALLLPKKIENLNPSKNPKENEICTQKITIKSVSSRKNQ
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCHHHHHEEEHHHHCCCCC
LFGLGFCEEWQENISFVFFHPRAWHFGICKVGKGLIFNAKLSRFNHTWQFNNPKILTSFE
EEECCCHHHHHCCCEEEEECCCCCEEHHHHHCCCEEEEEEHHHCCCEEEECCCEEEEEEC
GFSPKYQILGLKDAKIAAFIHKYLNYENLQESGIGDKYIHFLLNLHAYDEKSFFMFENLQ
CCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
NFSKDLKYIEIYNFLKRLKAKKTHFKAHQINVFNIANWLKDLPFSLTKDQLNALKDIEKD
HHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LHSKEARRRVIMGDVGCGKTLVLLGAALMVYPKQAILMAPTSILAHQLYEEAKKFLPDFM
HHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCHHHEECHHHHHHHHHHHHHHHHHHHHH
NILFIKGGKKEKDLEQNIQKANLIIGTHALIHLESHNAVLVMIDEQHRFGSAQREKIYSL
HEEEEECCCCCHHHHHHHHHHHEEEEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHH
SKQEFAPHFIQFSATPIPRTLSMIQSELLNFSFIKQMPFKKDITTYCIQNEDFSKLSEKI
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHH
KEEIAKNHQIIIIYPLVSASDNIPYLSLEQAKEYWQSHYEKVFVTHGKDKQKDEILERFR
HHHHHCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHH
DEGNILLSTTVVEVGISLPRLSMIVIVGAERLGLATLHQLRGRVGRVGLKSTCYLYTKLK
CCCCEEEEEHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EIPSRLKEFASTLDGFKIAELDLKNRLSGDLLDGFMQHGNEFKFFDFSKDEEILEKVKKD
HHHHHHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
LAKKMPN
HHHHCCC
>Mature Secondary Structure
MKIKESDFEFFKKLKIKSAIDLALLLPKKIENLNPSKNPKENEICTQKITIKSVSSRKNQ
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCHHHHHEEEHHHHCCCCC
LFGLGFCEEWQENISFVFFHPRAWHFGICKVGKGLIFNAKLSRFNHTWQFNNPKILTSFE
EEECCCHHHHHCCCEEEEECCCCCEEHHHHHCCCEEEEEEHHHCCCEEEECCCEEEEEEC
GFSPKYQILGLKDAKIAAFIHKYLNYENLQESGIGDKYIHFLLNLHAYDEKSFFMFENLQ
CCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
NFSKDLKYIEIYNFLKRLKAKKTHFKAHQINVFNIANWLKDLPFSLTKDQLNALKDIEKD
HHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LHSKEARRRVIMGDVGCGKTLVLLGAALMVYPKQAILMAPTSILAHQLYEEAKKFLPDFM
HHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCHHHEECHHHHHHHHHHHHHHHHHHHHH
NILFIKGGKKEKDLEQNIQKANLIIGTHALIHLESHNAVLVMIDEQHRFGSAQREKIYSL
HEEEEECCCCCHHHHHHHHHHHEEEEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHH
SKQEFAPHFIQFSATPIPRTLSMIQSELLNFSFIKQMPFKKDITTYCIQNEDFSKLSEKI
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHH
KEEIAKNHQIIIIYPLVSASDNIPYLSLEQAKEYWQSHYEKVFVTHGKDKQKDEILERFR
HHHHHCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHH
DEGNILLSTTVVEVGISLPRLSMIVIVGAERLGLATLHQLRGRVGRVGLKSTCYLYTKLK
CCCCEEEEEHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EIPSRLKEFASTLDGFKIAELDLKNRLSGDLLDGFMQHGNEFKFFDFSKDEEILEKVKKD
HHHHHHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
LAKKMPN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]