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The map label for this gene is miaB [H]

Identifier: 57237512

GI number: 57237512

Start: 460621

End: 461922

Strand: Reverse

Name: miaB [H]

Synonym: CJE0508

Alternate gene names: 57237512

Gene position: 461922-460621 (Counterclockwise)

Preceding gene: 57237513

Following gene: 57237511

Centisome position: 25.98

GC content: 32.18

Gene sequence:

>1302_bases
TTGAGTGCTAAAAAACTTTTTATCCAAACTTTAGGTTGTGCGATGAATGTTAGAGATTCTGAGCATATGATCGCAGAATT
AACACAAAAAGAAAATTACGCACTAACAGAAGATATCAAAGAAGCAGATCTTATTCTTATCAATACCTGTTCAGTGCGTG
AAAAACCTGTGCATAAACTTTTCTCAGAAGTAGGTGGTTTTGAAAAAGTTAAAAAAGAAGGTGCTAAAATAGGAGTTTGT
GGATGTACTGCATCGCATTTGGGAAATGAAATTTTCAAACGTGCTCCTTATGTGGATTTTGTTCTAGGAGCAAGAAATAT
TTCCAAAATCACTCAAGCTATAAAAACTCCAAAATTTATGGGCGTGGACATAGACTATGATGAAAGTGAATTTGCTTTTG
CAGATTTTAGAAATAGTATTTATAAATCCTATATTAATATCTCCATAGGTTGTGATAAACACTGCACTTATTGTATAGTG
CCACATACTAGAGGAGATGAAATTTCCATACCTTTTAACATTATCTACAAAGAAGCTCAAAAAGCTGTTGAAAAAGGTGC
TAAAGAGATTTTTTTGCTAGGACAAAATGTCAATAACTACGGCAAAAGATTTAGAAACGAGCATAAAAAAATGGACTTTT
CAGATCTTTTAGAAGAACTTAGCACCATAGAAGATTTAGAACGCATTCGCTTTACAAGTCCACATCCTTTGCATATGGAT
GATAAATTTTTAGAAGTTTTTGCCAATAATCCAAAAGTTTGCAAATCCATGCACATGCCTTTACAAAGCGGCTCAAGTGA
AATTTTAAAAGCTATGAAGCGTGGCTATACTAAAGAATGGTATTTAAATAGAGCTTTAAAACTTAGAGAACTTTGTCCAA
ATGTTAGCATTTCTACAGACATTATTGTAGCTTTTCCAGGGGAAAGTGAAAAAGATTTTGAAGAAACTATGGATGTTTTG
GAAAAAGTGCGTTTTGAACAAATTTTTTCTTTTAAATACTCCAAACGCCCTTTAACAAAAGCCGCAACCATGCCTAATCA
AATCGATGAAGAAACAGCTTCAAGAAGACTTAGCACCTTACAAAATCGTCATAGTGAAATTTTAGATGAAATTGTAAAAA
AACAAGAAAATAAAACTTTTAAAGTTTTATTTGAAGAATTAAGAGCAGGCAATAGCATTGCTGGAAGAACAGATAATAAT
TTTTTAGTTCAAGTTGAAGGTAGCGAAGAACTTTTAGGGCAATTTAAAGAAGTAAAAATAACTAATGCAAAGCGTATGGT
TTTATATGGGGAAATCGTTTAA

Upstream 100 bases:

>100_bases
CCCTTGTAAATCATTTTGAAAAAAAAGGAGTAAGCATTTATCATAGAACCGTAAAATACGGACTTGATGATAATGATTTT
ATGTATGAGGTACATATTCT

Downstream 100 bases:

>100_bases
AATCCATTGCTTAACATATATAATTTTCATCTTGCAATGGCTTATTTTTTTAAGCTGTAAAAAGCACTATAAAGGTGAAA
AAGTTGACCGTAAAGCTGGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKLFSEVGGFEKVKKEGAKIGVC
GCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFMGVDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIV
PHTRGDEISIPFNIIYKEAQKAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKEWYLNRALKLRELCPNVSISTDIIVAFPGESEKDFEETMDVL
EKVRFEQIFSFKYSKRPLTKAATMPNQIDEETASRRLSTLQNRHSEILDEIVKKQENKTFKVLFEELRAGNSIAGRTDNN
FLVQVEGSEELLGQFKEVKITNAKRMVLYGEIV

Sequences:

>Translated_433_residues
MSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKLFSEVGGFEKVKKEGAKIGVC
GCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFMGVDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIV
PHTRGDEISIPFNIIYKEAQKAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKEWYLNRALKLRELCPNVSISTDIIVAFPGESEKDFEETMDVL
EKVRFEQIFSFKYSKRPLTKAATMPNQIDEETASRRLSTLQNRHSEILDEIVKKQENKTFKVLFEELRAGNSIAGRTDNN
FLVQVEGSEELLGQFKEVKITNAKRMVLYGEIV
>Mature_432_residues
SAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKLFSEVGGFEKVKKEGAKIGVCG
CTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFMGVDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVP
HTRGDEISIPFNIIYKEAQKAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMDD
KFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKEWYLNRALKLRELCPNVSISTDIIVAFPGESEKDFEETMDVLE
KVRFEQIFSFKYSKRPLTKAATMPNQIDEETASRRLSTLQNRHSEILDEIVKKQENKTFKVLFEELRAGNSIAGRTDNNF
LVQVEGSEELLGQFKEVKITNAKRMVLYGEIV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=33.125, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=33.125, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=26.3274336283186, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=36.9614512471655, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787057, Length=442, Percent_Identity=28.2805429864253, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=29.0948275862069, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71996771, Length=362, Percent_Identity=27.6243093922652, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21356207, Length=480, Percent_Identity=28.5416666666667, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=29.1666666666667, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49540; Mature: 49408

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50926 TRAM ; PS00133 CARBOXYPEPT_ZN_2 ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCHHHHHHH
FSEVGGFEKVKKEGAKIGVCGCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFM
HHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCCCCEE
GVDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVPHTRGDEISIPFNIIYKEAQ
EEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCEEECHHHHHHHHHH
KAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
HHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKEWYLNRALKLRELCPNVSISTD
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
IIVAFPGESEKDFEETMDVLEKVRFEQIFSFKYSKRPLTKAATMPNQIDEETASRRLSTL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHH
QNRHSEILDEIVKKQENKTFKVLFEELRAGNSIAGRTDNNFLVQVEGSEELLGQFKEVKI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHEEE
TNAKRMVLYGEIV
CCCCEEEEEEECC
>Mature Secondary Structure 
SAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCHHHHHHH
FSEVGGFEKVKKEGAKIGVCGCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFM
HHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCCCCEE
GVDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVPHTRGDEISIPFNIIYKEAQ
EEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCEEECHHHHHHHHHH
KAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
HHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKEWYLNRALKLRELCPNVSISTD
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
IIVAFPGESEKDFEETMDVLEKVRFEQIFSFKYSKRPLTKAATMPNQIDEETASRRLSTL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHH
QNRHSEILDEIVKKQENKTFKVLFEELRAGNSIAGRTDNNFLVQVEGSEELLGQFKEVKI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHEEE
TNAKRMVLYGEIV
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA