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The map label for this gene is trpD [H]

Identifier: 57237401

GI number: 57237401

Start: 354331

End: 355932

Strand: Direct

Name: trpD [H]

Synonym: CJE0395

Alternate gene names: 57237401

Gene position: 354331-355932 (Clockwise)

Preceding gene: 57237400

Following gene: 57237402

Centisome position: 19.93

GC content: 30.77

Gene sequence:

>1602_bases
ATGATACTTTTAATTGATAATTATGATTCTTTTGTTTTTAATGTTAAAAGCATGTTAGAGCAACTCAGCAATGATGAAAT
TTTAGTAAGAAGAAATGATGCAATTTCTCTGAGTGAAATTAAAAATTTAAATCCTACTCATATTATTTTAAGTCCTGGTC
CAAAGCATCCAAGTCAAAGTGGGATTTGTCTTGAAATTTTTAAAGCAAGGCTTAATATCCCTGTTTTGGGCATTTGCTTA
GGACATCAAGCTTTAGCTTTAGCTTTTGATTCTTTAGTGGTTAAGATGCAAGAGCCTATGCATGCAAAAAATTCTTTGAT
AAAACAGTGCAGGGAAAATGAATTATTTTCTAATTTGCCTTCAAATTTTAGTGTAATGCGTTATCATTCTTTAGAGGTTA
AACAGCTTAGTGATGAGCTTGAAATTTTAGCCCTTGATGAAAAGGGTGTGATTATGGCTTTGGGACATAAAAATTTACCT
TATTATGGTGTGCAATTTCATCCTGAAAGCTATTTTAGTGAATATGGCTTACAACTTTTTTCTAATTTTTTAAAACAAGA
TATAAAAAAACCACAAAAACAAGAAAATCCTTTAAGTTTTTATTTGCAAAAAATGAGTGAAAATCATTTTTTACAAAGCG
ATGATTTTGAGCAAATTTGTAAGATTATTATGTCTAAAGATTATGAAATTTTACAAGTAGCGGCTTTGTTGATTTTAATC
ACTGAAAAGTCTTTAAACGAAAAATCTTTAAGTGCTTTTGTAAGACAAATTTTAAGATATTCTCAAACTTTTAGTGATGA
GAGTGAGATGATTGATATTTGTGGAACAGGTGGAGATGGTTTTAAAAGTATCAATGTTTCTACGACTAGTGCTTTTATTT
TAGCAGCTTTAGGAGTAAAAGTAGCCAAACACGGAAATCGTGCCATTTCAAGTTCTAGCGGAAGTACAGATGTGTTAGAA
GCTTTAAATATAACTACTCCAAACACTTTAGAATCAGTTTTAAAACAACTAAATAATCAAGGGCTTAGTTTTTTACATGC
TCCATTTTTTCATCCTTTGGTAGGAGAACTTAAAGAGGTCAGATCAAGACTTGGAGTAAGAACGGTTTTTAATGTTTTAG
GTCCTTTATTGCACCCTAATTTAAAGTTAAAATATCAACTTATGGGAAATTATCACGCACCTGTGCATAGGCTTTTGATA
GAGGTTTTAAAAAATTTAGGACGCAAAAAAGCTTTAGTGGTAAGAGGAAATGATGGTATGGATGAGCTTAGTATTTGCGA
TGAAAGTAAAATTTATGAGCTTTGTGAAGGAGAAATTTTAGAGTATTCTATCTGTCCTGAACAATTTGGTTTTAAAAGAG
CTTTTCATAGTGAAATCATAGGATCTAGTGCATATGACAACGCTAAAGATTTAAAAGATATTTTAAGCGGTAGAATGCAA
GGGGCAAAATTTGATTTGGTAGTTTTAAATGCTATGTTTGCACTTTATACAGCCAATAAAGCAAGTTCGCCTTTAGTGGC
TAAAGATATGATTTTAGAAGCCATTTATTCAGGTAAGGTTATAGAATATTTTAAGGAGTATCAAGCTTATGCTAAAGCTT
AA

Upstream 100 bases:

>100_bases
GTATTGTTTTGCAAAGTGAGAGTCAAAAAGAATATGCAGAAATTTGTGCTAAACGCAAGGCGCTTTTGGTAGCTTTTGAA
AATTTAAAAAAGGAAAATCA

Downstream 100 bases:

>100_bases
AATTTGTGGTATTAAAGATGAAAAAAATGCCAAAGATTTAGCTTTTTTAAATATTGATTTTTTTGGACTTATTTTTGCTA
AAAGTCCAAGAAGAGTGAGT

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amidotransferase; Anthranilate phosphoribosyltransferase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MILLIDNYDSFVFNVKSMLEQLSNDEILVRRNDAISLSEIKNLNPTHIILSPGPKHPSQSGICLEIFKARLNIPVLGICL
GHQALALAFDSLVVKMQEPMHAKNSLIKQCRENELFSNLPSNFSVMRYHSLEVKQLSDELEILALDEKGVIMALGHKNLP
YYGVQFHPESYFSEYGLQLFSNFLKQDIKKPQKQENPLSFYLQKMSENHFLQSDDFEQICKIIMSKDYEILQVAALLILI
TEKSLNEKSLSAFVRQILRYSQTFSDESEMIDICGTGGDGFKSINVSTTSAFILAALGVKVAKHGNRAISSSSGSTDVLE
ALNITTPNTLESVLKQLNNQGLSFLHAPFFHPLVGELKEVRSRLGVRTVFNVLGPLLHPNLKLKYQLMGNYHAPVHRLLI
EVLKNLGRKKALVVRGNDGMDELSICDESKIYELCEGEILEYSICPEQFGFKRAFHSEIIGSSAYDNAKDLKDILSGRMQ
GAKFDLVVLNAMFALYTANKASSPLVAKDMILEAIYSGKVIEYFKEYQAYAKA

Sequences:

>Translated_533_residues
MILLIDNYDSFVFNVKSMLEQLSNDEILVRRNDAISLSEIKNLNPTHIILSPGPKHPSQSGICLEIFKARLNIPVLGICL
GHQALALAFDSLVVKMQEPMHAKNSLIKQCRENELFSNLPSNFSVMRYHSLEVKQLSDELEILALDEKGVIMALGHKNLP
YYGVQFHPESYFSEYGLQLFSNFLKQDIKKPQKQENPLSFYLQKMSENHFLQSDDFEQICKIIMSKDYEILQVAALLILI
TEKSLNEKSLSAFVRQILRYSQTFSDESEMIDICGTGGDGFKSINVSTTSAFILAALGVKVAKHGNRAISSSSGSTDVLE
ALNITTPNTLESVLKQLNNQGLSFLHAPFFHPLVGELKEVRSRLGVRTVFNVLGPLLHPNLKLKYQLMGNYHAPVHRLLI
EVLKNLGRKKALVVRGNDGMDELSICDESKIYELCEGEILEYSICPEQFGFKRAFHSEIIGSSAYDNAKDLKDILSGRMQ
GAKFDLVVLNAMFALYTANKASSPLVAKDMILEAIYSGKVIEYFKEYQAYAKA
>Mature_533_residues
MILLIDNYDSFVFNVKSMLEQLSNDEILVRRNDAISLSEIKNLNPTHIILSPGPKHPSQSGICLEIFKARLNIPVLGICL
GHQALALAFDSLVVKMQEPMHAKNSLIKQCRENELFSNLPSNFSVMRYHSLEVKQLSDELEILALDEKGVIMALGHKNLP
YYGVQFHPESYFSEYGLQLFSNFLKQDIKKPQKQENPLSFYLQKMSENHFLQSDDFEQICKIIMSKDYEILQVAALLILI
TEKSLNEKSLSAFVRQILRYSQTFSDESEMIDICGTGGDGFKSINVSTTSAFILAALGVKVAKHGNRAISSSSGSTDVLE
ALNITTPNTLESVLKQLNNQGLSFLHAPFFHPLVGELKEVRSRLGVRTVFNVLGPLLHPNLKLKYQLMGNYHAPVHRLLI
EVLKNLGRKKALVVRGNDGMDELSICDESKIYELCEGEILEYSICPEQFGFKRAFHSEIIGSSAYDNAKDLKDILSGRMQ
GAKFDLVVLNAMFALYTANKASSPLVAKDMILEAIYSGKVIEYFKEYQAYAKA

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0547

COG function: function code E; Anthranilate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=486, Percent_Identity=34.3621399176955, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI1789760, Length=189, Percent_Identity=44.4444444444444, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322638, Length=189, Percent_Identity=41.7989417989418, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320561, Length=284, Percent_Identity=29.5774647887324, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324361, Length=212, Percent_Identity=33.4905660377358, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005940
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR000312
- InterPro:   IPR017459
- InterPro:   IPR020072
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase; PF02885 Glycos_trans_3N; PF00591 Glycos_transf_3 [H]

EC number: =4.1.3.27; =2.4.2.18 [H]

Molecular weight: Translated: 59851; Mature: 59851

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLIDNYDSFVFNVKSMLEQLSNDEILVRRNDAISLSEIKNLNPTHIILSPGPKHPSQS
CEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHCCCCCCEEEECCCCCCCCCC
GICLEIFKARLNIPVLGICLGHQALALAFDSLVVKMQEPMHAKNSLIKQCRENELFSNLP
CHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
SNFSVMRYHSLEVKQLSDELEILALDEKGVIMALGHKNLPYYGVQFHPESYFSEYGLQLF
CCCEEEEEECCHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHH
SNFLKQDIKKPQKQENPLSFYLQKMSENHFLQSDDFEQICKIIMSKDYEILQVAALLILI
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
TEKSLNEKSLSAFVRQILRYSQTFSDESEMIDICGTGGDGFKSINVSTTSAFILAALGVK
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHH
VAKHGNRAISSSSGSTDVLEALNITTPNTLESVLKQLNNQGLSFLHAPFFHPLVGELKEV
HHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RSRLGVRTVFNVLGPLLHPNLKLKYQLMGNYHAPVHRLLIEVLKNLGRKKALVVRGNDGM
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
DELSICDESKIYELCEGEILEYSICPEQFGFKRAFHSEIIGSSAYDNAKDLKDILSGRMQ
CHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
GAKFDLVVLNAMFALYTANKASSPLVAKDMILEAIYSGKVIEYFKEYQAYAKA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILLIDNYDSFVFNVKSMLEQLSNDEILVRRNDAISLSEIKNLNPTHIILSPGPKHPSQS
CEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHCCCCCCEEEECCCCCCCCCC
GICLEIFKARLNIPVLGICLGHQALALAFDSLVVKMQEPMHAKNSLIKQCRENELFSNLP
CHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
SNFSVMRYHSLEVKQLSDELEILALDEKGVIMALGHKNLPYYGVQFHPESYFSEYGLQLF
CCCEEEEEECCHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHH
SNFLKQDIKKPQKQENPLSFYLQKMSENHFLQSDDFEQICKIIMSKDYEILQVAALLILI
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
TEKSLNEKSLSAFVRQILRYSQTFSDESEMIDICGTGGDGFKSINVSTTSAFILAALGVK
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHH
VAKHGNRAISSSSGSTDVLEALNITTPNTLESVLKQLNNQGLSFLHAPFFHPLVGELKEV
HHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RSRLGVRTVFNVLGPLLHPNLKLKYQLMGNYHAPVHRLLIEVLKNLGRKKALVVRGNDGM
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
DELSICDESKIYELCEGEILEYSICPEQFGFKRAFHSEIIGSSAYDNAKDLKDILSGRMQ
CHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
GAKFDLVVLNAMFALYTANKASSPLVAKDMILEAIYSGKVIEYFKEYQAYAKA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685830; 10360571; 7556082 [H]