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The map label for this gene is trpE [H]

Identifier: 57237400

GI number: 57237400

Start: 353084

End: 354334

Strand: Direct

Name: trpE [H]

Synonym: CJE0394

Alternate gene names: 57237400

Gene position: 353084-354334 (Clockwise)

Preceding gene: 57237398

Following gene: 57237401

Centisome position: 19.86

GC content: 29.42

Gene sequence:

>1251_bases
ATGTTTGTTAAAAATGTAAATTTTTATTACAGACAAATCCTTGAAAAATTTGAAAATTCTTATTTTGCAGAGGATTTGAC
TAAGGTAATTATAGGGATTGATTGTGATTATTTAGATGCTAATGAATTATCCTTTAGTGAGTTTAAGGCAAAATATTATG
AAGCTTTAAGCAAGAATAAAATTTGCGACTTTGCTGGATTTTTTGGAGTTTTTAGTGCAAATTTTGTATCTTTGTTTGAA
AAAATTCCACTTTCTTCTAAGAAAAATTATGATTTTCCTTTATTTTTATTTGCCAATGCTAAAGCTTATTTGATTTATGA
GAAAAATAGTAAGATGTTTTTTAAATTTGGCGCTTCAAAATATTTTGAATATTTAAAAGATGATATTGAGCCAATAAAAA
CAAAGCAAAAAAATGCTTTTGAAATTTTAAACTCTTTAGAAGATGAAGAAAATGATTTTTTAAAAATGTGCGAAAAAGCC
AAAGAATATCTTTTAAGTGGAGATATTTTTCAAGTGGTCTTAAGTAAGCAACTTTGCATCAAGCATCAAGTAAATGCTTT
TGACTATTATGAGAGTTTAAGTACTTTAAATCCAAGCGCTTATATGTTTTATTTTCCAAGTAAATATGGTGTTGTTTTGG
GTTCTTCTCCAGAGTTTTTATTAAAAATTAAAAAAAGAGAGATTTATTTAGCCCCTATTGCTGGAACTAGAAATTTGGAA
AATAACTGTGATCTTTTGGCTTTAGAAAAAGATCTTTTAAGTGATGAAAAAGAACTTAGTGAACATAAGATGCTTGTAGA
TTTAGCAAGAAATGATGCTTCTAAATTTGGCACTCAAACAAGAGTGGAAAATCTTTTTTCTATTATTAAAAATAAATTTG
TAATGCACATAGTGAGTGAAGTGTATGCCAATATGAAAGAAGATGCGAGTATTTTTGATGTGATTGAAGCAGTTTTTCCA
GCGGGAACTTTAAGCGGAGCTCCTAAAATAAGAGCTTTAGAGATCACTAGCGAGCTTGAAGATTGCGATCGTGGAATTTA
TGGTGGTGCGGTAGGATTTTTAAATTTTAACGAAGACATAACTTTAGCGATTTTAATCCGTTGCGCTTTTTTTACTCAAG
ATAAGGCTTATTTGGCAAGTGGGGCGGGTATTGTTTTGCAAAGTGAGAGTCAAAAAGAATATGCAGAAATTTGTGCTAAA
CGCAAGGCGCTTTTGGTAGCTTTTGAAAATTTAAAAAAGGAAAATCAATGA

Upstream 100 bases:

>100_bases
CTTACACACACTCTTCGCTCTTGGCGTTTCTAAAATTCTTTTTATCTAAATTTTTTCTTTAATATTATTTTAATTTATTT
TTTTTAAAAAAAGGTTTATT

Downstream 100 bases:

>100_bases
TACTTTTAATTGATAATTATGATTCTTTTGTTTTTAATGTTAAAAGCATGTTAGAGCAACTCAGCAATGATGAAATTTTA
GTAAGAAGAAATGATGCAAT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFVKNVNFYYRQILEKFENSYFAEDLTKVIIGIDCDYLDANELSFSEFKAKYYEALSKNKICDFAGFFGVFSANFVSLFE
KIPLSSKKNYDFPLFLFANAKAYLIYEKNSKMFFKFGASKYFEYLKDDIEPIKTKQKNAFEILNSLEDEENDFLKMCEKA
KEYLLSGDIFQVVLSKQLCIKHQVNAFDYYESLSTLNPSAYMFYFPSKYGVVLGSSPEFLLKIKKREIYLAPIAGTRNLE
NNCDLLALEKDLLSDEKELSEHKMLVDLARNDASKFGTQTRVENLFSIIKNKFVMHIVSEVYANMKEDASIFDVIEAVFP
AGTLSGAPKIRALEITSELEDCDRGIYGGAVGFLNFNEDITLAILIRCAFFTQDKAYLASGAGIVLQSESQKEYAEICAK
RKALLVAFENLKKENQ

Sequences:

>Translated_416_residues
MFVKNVNFYYRQILEKFENSYFAEDLTKVIIGIDCDYLDANELSFSEFKAKYYEALSKNKICDFAGFFGVFSANFVSLFE
KIPLSSKKNYDFPLFLFANAKAYLIYEKNSKMFFKFGASKYFEYLKDDIEPIKTKQKNAFEILNSLEDEENDFLKMCEKA
KEYLLSGDIFQVVLSKQLCIKHQVNAFDYYESLSTLNPSAYMFYFPSKYGVVLGSSPEFLLKIKKREIYLAPIAGTRNLE
NNCDLLALEKDLLSDEKELSEHKMLVDLARNDASKFGTQTRVENLFSIIKNKFVMHIVSEVYANMKEDASIFDVIEAVFP
AGTLSGAPKIRALEITSELEDCDRGIYGGAVGFLNFNEDITLAILIRCAFFTQDKAYLASGAGIVLQSESQKEYAEICAK
RKALLVAFENLKKENQ
>Mature_416_residues
MFVKNVNFYYRQILEKFENSYFAEDLTKVIIGIDCDYLDANELSFSEFKAKYYEALSKNKICDFAGFFGVFSANFVSLFE
KIPLSSKKNYDFPLFLFANAKAYLIYEKNSKMFFKFGASKYFEYLKDDIEPIKTKQKNAFEILNSLEDEENDFLKMCEKA
KEYLLSGDIFQVVLSKQLCIKHQVNAFDYYESLSTLNPSAYMFYFPSKYGVVLGSSPEFLLKIKKREIYLAPIAGTRNLE
NNCDLLALEKDLLSDEKELSEHKMLVDLARNDASKFGTQTRVENLFSIIKNKFVMHIVSEVYANMKEDASIFDVIEAVFP
AGTLSGAPKIRALEITSELEDCDRGIYGGAVGFLNFNEDITLAILIRCAFFTQDKAYLASGAGIVLQSESQKEYAEICAK
RKALLVAFENLKKENQ

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=371, Percent_Identity=33.6927223719677, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1788114, Length=254, Percent_Identity=30.3149606299213, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1786809, Length=334, Percent_Identity=27.8443113772455, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082077, Length=264, Percent_Identity=23.1060606060606, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=290, Percent_Identity=34.8275862068965, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324361, Length=349, Percent_Identity=24.6418338108883, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 47607; Mature: 47607

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVKNVNFYYRQILEKFENSYFAEDLTKVIIGIDCDYLDANELSFSEFKAKYYEALSKNK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
ICDFAGFFGVFSANFVSLFEKIPLSSKKNYDFPLFLFANAKAYLIYEKNSKMFFKFGASK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCCCEEEEECHHH
YFEYLKDDIEPIKTKQKNAFEILNSLEDEENDFLKMCEKAKEYLLSGDIFQVVLSKQLCI
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KHQVNAFDYYESLSTLNPSAYMFYFPSKYGVVLGSSPEFLLKIKKREIYLAPIAGTRNLE
HHCCCHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCHHEEEEEECEEEEEECCCCCCCC
NNCDLLALEKDLLSDEKELSEHKMLVDLARNDASKFGTQTRVENLFSIIKNKFVMHIVSE
CCCCEEEECHHHCCCHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
VYANMKEDASIFDVIEAVFPAGTLSGAPKIRALEITSELEDCDRGIYGGAVGFLNFNEDI
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEECCCCH
TLAILIRCAFFTQDKAYLASGAGIVLQSESQKEYAEICAKRKALLVAFENLKKENQ
HHHHHHHHHHHCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFVKNVNFYYRQILEKFENSYFAEDLTKVIIGIDCDYLDANELSFSEFKAKYYEALSKNK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
ICDFAGFFGVFSANFVSLFEKIPLSSKKNYDFPLFLFANAKAYLIYEKNSKMFFKFGASK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCCCEEEEECHHH
YFEYLKDDIEPIKTKQKNAFEILNSLEDEENDFLKMCEKAKEYLLSGDIFQVVLSKQLCI
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KHQVNAFDYYESLSTLNPSAYMFYFPSKYGVVLGSSPEFLLKIKKREIYLAPIAGTRNLE
HHCCCHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCHHEEEEEECEEEEEECCCCCCCC
NNCDLLALEKDLLSDEKELSEHKMLVDLARNDASKFGTQTRVENLFSIIKNKFVMHIVSE
CCCCEEEECHHHCCCHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
VYANMKEDASIFDVIEAVFPAGTLSGAPKIRALEITSELEDCDRGIYGGAVGFLNFNEDI
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEECCCCH
TLAILIRCAFFTQDKAYLASGAGIVLQSESQKEYAEICAKRKALLVAFENLKKENQ
HHHHHHHHHHHCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]