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The map label for this gene is ubiE [H]

Identifier: 57237375

GI number: 57237375

Start: 331420

End: 332127

Strand: Direct

Name: ubiE [H]

Synonym: CJE0369

Alternate gene names: 57237375

Gene position: 331420-332127 (Clockwise)

Preceding gene: 57237374

Following gene: 57237376

Centisome position: 18.64

GC content: 30.51

Gene sequence:

>708_bases
ATGCAAAAACAAGAAAAAATTATAGAAATGTTTAATCAAATTGCACCTACTTATGATAAGGCGAATAGAATTTTAAGCTT
TGGAGCTGATGTAGCGTGGCGCAAAAAAGCTTGTCAAAGAGTGATGAGTTTGTATTTAAAAAAAGATTTAAAAATCGCTG
ATATTGCCTGTGGCACAGGGGATATGATAGAGATTTGGCAAGAGAGTGCTTTAAAAATGGAAAAAAATATTCTAAATATT
AAAGGTATTGATCCAAGTAGTGGCATGCTAAATGTTGCAAAAGAAAAATTTCCAAATGTCGAATTTATAGAAGCAGGGGC
GCAAAATTTACCTTTAGAAAGTCAAAGTTTAGATATTTTAAGCATTAGTTATGGCATTCGCAATGTTGTAGAAAGACAAA
AAGCCTTAAGTGAATTTGCTAGAGTACTTCAAAAAGATGGTATTTTGGTAGTTTTGGAATTTACAAAAAGAGAAAAGGGT
GGATTTATAGCAGCTTGTAGAGATTTTTATCTTAAAAATATTTTACCAAGTATAGGTGGGATAATCAGTAAAAATAAAAG
TGCTTATGAGTATTTGCCAAACTCTATTGAAGGATTTTTAAGTAAGGAAGAGTTCATCTTAGAACTTAAAAATGCTGGTT
TTGAAATACTTGATTATAAAAGTTTTAGCTTTGGCGTGAGTTCAATGTTTATAGCAAAAAAACTATGA

Upstream 100 bases:

>100_bases
GGGATAAAAATATTTTTTCGAAAATAATCAATAATAAAAATAAATTTTGCAAATTGCTTAAAAAATCAAGTAAAAATTTG
AAAATTTACAAAGGATAAAA

Downstream 100 bases:

>100_bases
CACCAACAGAATTAAATCTTAAAGCAAAGGCTTTATTAGAAACACATTTTGATGATATAGTTTTAAGTGGAGAAATTTCA
AAAATTACTTTGCATGGTTC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MQKQEKIIEMFNQIAPTYDKANRILSFGADVAWRKKACQRVMSLYLKKDLKIADIACGTGDMIEIWQESALKMEKNILNI
KGIDPSSGMLNVAKEKFPNVEFIEAGAQNLPLESQSLDILSISYGIRNVVERQKALSEFARVLQKDGILVVLEFTKREKG
GFIAACRDFYLKNILPSIGGIISKNKSAYEYLPNSIEGFLSKEEFILELKNAGFEILDYKSFSFGVSSMFIAKKL

Sequences:

>Translated_235_residues
MQKQEKIIEMFNQIAPTYDKANRILSFGADVAWRKKACQRVMSLYLKKDLKIADIACGTGDMIEIWQESALKMEKNILNI
KGIDPSSGMLNVAKEKFPNVEFIEAGAQNLPLESQSLDILSISYGIRNVVERQKALSEFARVLQKDGILVVLEFTKREKG
GFIAACRDFYLKNILPSIGGIISKNKSAYEYLPNSIEGFLSKEEFILELKNAGFEILDYKSFSFGVSSMFIAKKL
>Mature_235_residues
MQKQEKIIEMFNQIAPTYDKANRILSFGADVAWRKKACQRVMSLYLKKDLKIADIACGTGDMIEIWQESALKMEKNILNI
KGIDPSSGMLNVAKEKFPNVEFIEAGAQNLPLESQSLDILSISYGIRNVVERQKALSEFARVLQKDGILVVLEFTKREKG
GFIAACRDFYLKNILPSIGGIISKNKSAYEYLPNSIEGFLSKEEFILELKNAGFEILDYKSFSFGVSSMFIAKKL

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=269, Percent_Identity=26.7657992565056, Blast_Score=97, Evalue=8e-21,
Organism=Escherichia coli, GI48994983, Length=238, Percent_Identity=30.672268907563, Blast_Score=120, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17557039, Length=237, Percent_Identity=29.535864978903, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323526, Length=252, Percent_Identity=29.7619047619048, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24641702, Length=249, Percent_Identity=28.5140562248996, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26500; Mature: 26500

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQEKIIEMFNQIAPTYDKANRILSFGADVAWRKKACQRVMSLYLKKDLKIADIACGTG
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
DMIEIWQESALKMEKNILNIKGIDPSSGMLNVAKEKFPNVEFIEAGAQNLPLESQSLDIL
HHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEE
SISYGIRNVVERQKALSEFARVLQKDGILVVLEFTKREKGGFIAACRDFYLKNILPSIGG
EHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
IISKNKSAYEYLPNSIEGFLSKEEFILELKNAGFEILDYKSFSFGVSSMFIAKKL
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCHHHHHHHHCC
>Mature Secondary Structure
MQKQEKIIEMFNQIAPTYDKANRILSFGADVAWRKKACQRVMSLYLKKDLKIADIACGTG
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
DMIEIWQESALKMEKNILNIKGIDPSSGMLNVAKEKFPNVEFIEAGAQNLPLESQSLDIL
HHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEE
SISYGIRNVVERQKALSEFARVLQKDGILVVLEFTKREKGGFIAACRDFYLKNILPSIGG
EHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
IISKNKSAYEYLPNSIEGFLSKEEFILELKNAGFEILDYKSFSFGVSSMFIAKKL
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA