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The map label for this gene is eptA [H]

Identifier: 57237313

GI number: 57237313

Start: 269288

End: 270826

Strand: Direct

Name: eptA [H]

Synonym: CJE0306

Alternate gene names: 57237313

Gene position: 269288-270826 (Clockwise)

Preceding gene: 57237311

Following gene: 57237314

Centisome position: 15.15

GC content: 27.03

Gene sequence:

>1539_bases
ATGCTTAGATTAACTTGGTTTCAGTTTACTTTTTTTAATTCTTTGATGATTGCAATATTAAATTTTAATTTATTTTATTT
TGTGTATGAAAAAAATACACAAAACTGGTTTATAACTTTTGTTTTTATTGTGGCCTATTTTGCCCTTGTTCACGTGATTT
GCTCTTTGCTTTTTATAAAGTTTTTTACTAAATTTTTTTCTATATTATTTATAATATCATCTTTTTTAAGTGTTTACTTT
ATTAGTTTTTATGGGGTGCTTATAGATTCTGATATGATACAAAATGTTGTTCAAACAGATATCAAAGAAGTAAAAGATTT
GTTAAATTTAAAATTGATCTTATTTGTTGTTTTGGCTTTATTGTTGGTTTTTTATGTTGTTAAAGTAAAAATTGATTATT
ATGGTAGTTTTAAATCCCATCTAAAAATTAAAATTATCAATATAATATCAGGTTTGATTGTTGTTTGTGCGATTTTAATC
CCTTTAAGTAAAACTTTCTTGCCTTTTTTTAGAAACTATAATGAAATAAGAATGTATAATACTCCTTTTTATCAAATTTA
TTCCGTATATCGCTATTATGTTCGTTTTGTAAAAACTAAGCCAGAATTTAAAACTATAGCTAATGATGCTTATAGAGAAA
ACAATCACACTAAAAAACTTTTAGTTTTGGTGGTGGGTGAAACCGCTAGAGCGGCAAATTACTCTTTGGGTGGATATACT
AAAAATGATACCAATTTTTATACCAAAAAAGATAATGTAGTCTTTTTTGATAATTTTTCATCATGTGGAACAGCAACAGC
GGTAAGTTTGCCTTGTATGTTTTCTATTTCTAAGCGTGAAAATTATTCAAGTTCAGAATTTCAAGAAAATGCGATGGATG
TACTTTATAAAACAGGTGTAGATGCTGCATGGTTTGATAATAATTCTGGTGGTTGTAAAGGGGTTTGCGATAGATTAGCT
TATAAGCAAAAGCTTTCTAGTGATTTGGATGAAAATTTACTCGCCCCTTTTAAAGAAAAATTAAATCATTTAAGTGATCA
AAATATAATAGTTCTTCATTTGCAAGGTTCTCATGGGCCAACTTATTATAAACGCTATCCAAGTGAATTTAAAAAATTTA
CTCCAACTTGTGATACTAATGAGCTTTCAAAATGTGATAGCGAGGCTTTAATCAACACTTATGACAATACTTTACTTTAT
ACAGATTATCTTTTAAGTGAGATTATAAAACTATTAAAAGAGCAAAAAAGCTATGAAAGTTCTTTGTTTTATCTTTCAGA
TCATGGTGAAAGTTTGGGTGAAAATGATATTTATCTTCATGGTATGCCTTATGCTATAGCGCCAAGTTATCAAACTCATA
TTCCTGCTATTTTTTGGAGCAATGATAAAAATTTAATGAATTTAGCTAAAGAGTATAAAGGTTTAAAACTTTCTCAAGAT
AATCTTTTTAGTACTCTTTTAGGATATTTTGATGTAAAAACAAGTGTTTATGAACCAGAATATGATTTATTAAATCCTAA
ACTTAAGGCAAATCCATGA

Upstream 100 bases:

>100_bases
TCATTAAAAGTGTAAATCATAACATAAAAAGATTTAAAACTACAATCCATTTAAAAAAATTCAATAAAATTAAAAGCTTA
AATTTGAAGGTGGGATTTAT

Downstream 100 bases:

>100_bases
AGCCTAAATATCATTTTTTAAATAACGCAAGATATGCTTTAGAAGGGCTTTTTGCTTTATTTAAAAATGAAATGGCGTTT
AAAATTGAACTTTGCATTAT

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLRLTWFQFTFFNSLMIAILNFNLFYFVYEKNTQNWFITFVFIVAYFALVHVICSLLFIKFFTKFFSILFIISSFLSVYF
ISFYGVLIDSDMIQNVVQTDIKEVKDLLNLKLILFVVLALLLVFYVVKVKIDYYGSFKSHLKIKIINIISGLIVVCAILI
PLSKTFLPFFRNYNEIRMYNTPFYQIYSVYRYYVRFVKTKPEFKTIANDAYRENNHTKKLLVLVVGETARAANYSLGGYT
KNDTNFYTKKDNVVFFDNFSSCGTATAVSLPCMFSISKRENYSSSEFQENAMDVLYKTGVDAAWFDNNSGGCKGVCDRLA
YKQKLSSDLDENLLAPFKEKLNHLSDQNIIVLHLQGSHGPTYYKRYPSEFKKFTPTCDTNELSKCDSEALINTYDNTLLY
TDYLLSEIIKLLKEQKSYESSLFYLSDHGESLGENDIYLHGMPYAIAPSYQTHIPAIFWSNDKNLMNLAKEYKGLKLSQD
NLFSTLLGYFDVKTSVYEPEYDLLNPKLKANP

Sequences:

>Translated_512_residues
MLRLTWFQFTFFNSLMIAILNFNLFYFVYEKNTQNWFITFVFIVAYFALVHVICSLLFIKFFTKFFSILFIISSFLSVYF
ISFYGVLIDSDMIQNVVQTDIKEVKDLLNLKLILFVVLALLLVFYVVKVKIDYYGSFKSHLKIKIINIISGLIVVCAILI
PLSKTFLPFFRNYNEIRMYNTPFYQIYSVYRYYVRFVKTKPEFKTIANDAYRENNHTKKLLVLVVGETARAANYSLGGYT
KNDTNFYTKKDNVVFFDNFSSCGTATAVSLPCMFSISKRENYSSSEFQENAMDVLYKTGVDAAWFDNNSGGCKGVCDRLA
YKQKLSSDLDENLLAPFKEKLNHLSDQNIIVLHLQGSHGPTYYKRYPSEFKKFTPTCDTNELSKCDSEALINTYDNTLLY
TDYLLSEIIKLLKEQKSYESSLFYLSDHGESLGENDIYLHGMPYAIAPSYQTHIPAIFWSNDKNLMNLAKEYKGLKLSQD
NLFSTLLGYFDVKTSVYEPEYDLLNPKLKANP
>Mature_512_residues
MLRLTWFQFTFFNSLMIAILNFNLFYFVYEKNTQNWFITFVFIVAYFALVHVICSLLFIKFFTKFFSILFIISSFLSVYF
ISFYGVLIDSDMIQNVVQTDIKEVKDLLNLKLILFVVLALLLVFYVVKVKIDYYGSFKSHLKIKIINIISGLIVVCAILI
PLSKTFLPFFRNYNEIRMYNTPFYQIYSVYRYYVRFVKTKPEFKTIANDAYRENNHTKKLLVLVVGETARAANYSLGGYT
KNDTNFYTKKDNVVFFDNFSSCGTATAVSLPCMFSISKRENYSSSEFQENAMDVLYKTGVDAAWFDNNSGGCKGVCDRLA
YKQKLSSDLDENLLAPFKEKLNHLSDQNIIVLHLQGSHGPTYYKRYPSEFKKFTPTCDTNELSKCDSEALINTYDNTLLY
TDYLLSEIIKLLKEQKSYESSLFYLSDHGESLGENDIYLHGMPYAIAPSYQTHIPAIFWSNDKNLMNLAKEYKGLKLSQD
NLFSTLLGYFDVKTSVYEPEYDLLNPKLKANP

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=501, Percent_Identity=39.5209580838323, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI87082286, Length=476, Percent_Identity=25.4201680672269, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI87082223, Length=447, Percent_Identity=25.7270693512304, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790392, Length=320, Percent_Identity=25, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59414; Mature: 59414

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLTWFQFTFFNSLMIAILNFNLFYFVYEKNTQNWFITFVFIVAYFALVHVICSLLFIK
CCEEEEEHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FFTKFFSILFIISSFLSVYFISFYGVLIDSDMIQNVVQTDIKEVKDLLNLKLILFVVLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVFYVVKVKIDYYGSFKSHLKIKIINIISGLIVVCAILIPLSKTFLPFFRNYNEIRMYN
HHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
TPFYQIYSVYRYYVRFVKTKPEFKTIANDAYRENNHTKKLLVLVVGETARAANYSLGGYT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCC
KNDTNFYTKKDNVVFFDNFSSCGTATAVSLPCMFSISKRENYSSSEFQENAMDVLYKTGV
CCCCCEEEECCCEEEEECCCCCCCHHHEECCCEEEEHHCCCCCCHHHHHHHHHHHHHCCC
DAAWFDNNSGGCKGVCDRLAYKQKLSSDLDENLLAPFKEKLNHLSDQNIIVLHLQGSHGP
CEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
TYYKRYPSEFKKFTPTCDTNELSKCDSEALINTYDNTLLYTDYLLSEIIKLLKEQKSYES
HHHHHCCHHHHHCCCCCCCCHHHHCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCHHC
SLFYLSDHGESLGENDIYLHGMPYAIAPSYQTHIPAIFWSNDKNLMNLAKEYKGLKLSQD
EEEEEECCCCCCCCCCEEEECCCCEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHH
NLFSTLLGYFDVKTSVYEPEYDLLNPKLKANP
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MLRLTWFQFTFFNSLMIAILNFNLFYFVYEKNTQNWFITFVFIVAYFALVHVICSLLFIK
CCEEEEEHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FFTKFFSILFIISSFLSVYFISFYGVLIDSDMIQNVVQTDIKEVKDLLNLKLILFVVLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVFYVVKVKIDYYGSFKSHLKIKIINIISGLIVVCAILIPLSKTFLPFFRNYNEIRMYN
HHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
TPFYQIYSVYRYYVRFVKTKPEFKTIANDAYRENNHTKKLLVLVVGETARAANYSLGGYT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCC
KNDTNFYTKKDNVVFFDNFSSCGTATAVSLPCMFSISKRENYSSSEFQENAMDVLYKTGV
CCCCCEEEECCCEEEEECCCCCCCHHHEECCCEEEEHHCCCCCCHHHHHHHHHHHHHCCC
DAAWFDNNSGGCKGVCDRLAYKQKLSSDLDENLLAPFKEKLNHLSDQNIIVLHLQGSHGP
CEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
TYYKRYPSEFKKFTPTCDTNELSKCDSEALINTYDNTLLYTDYLLSEIIKLLKEQKSYES
HHHHHCCHHHHHCCCCCCCCHHHHCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCHHC
SLFYLSDHGESLGENDIYLHGMPYAIAPSYQTHIPAIFWSNDKNLMNLAKEYKGLKLSQD
EEEEEECCCCCCCCCCEEEECCCCEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHH
NLFSTLLGYFDVKTSVYEPEYDLLNPKLKANP
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]