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The map label for this gene is xth [H]

Identifier: 57237312

GI number: 57237312

Start: 268412

End: 269191

Strand: Reverse

Name: xth [H]

Synonym: CJE0305

Alternate gene names: 57237312

Gene position: 269191-268412 (Counterclockwise)

Preceding gene: 57237318

Following gene: 57237308

Centisome position: 15.14

GC content: 30.13

Gene sequence:

>780_bases
ATGAAAATTTTAAGGAAAATAATGAAACTACTTTCATGGAATGTCAATGGCTTAAGAGCTATTTGTGATAAAAATGCACT
TGATTGGATAGCACAAGAACAAATTGATTTTATAGGTTTTCAAGAAATCAAAGCACATGAAGATAAATTTCCTAAAAAAA
TTTATGAATATCCTTTTAAACACATGTATTTTAATTCTGCTAAAAGAGCGGGGTATTCTGGGGTAATGAGTCTTTGTAAT
TTTAATAGCGAAGTAAAAAAATGCGAATTTTTTGATGATGAAGAAGGTAGGGTTTTAGAACACCGCTTTAAAAATATTGC
ACTTTTTAATATCTATTTTCCAAATGGTCAAAAAGATGAAGAGCGTTTAAATTTTAAGATGCAATTTTATGCAGATTTTT
TAGTGTATTTGGATAAGCTTTTAAAGGATGGATTTGAAATTATCATTTGCGGTGATGTCAATACCGCTCATAAAGAAATC
GATCTTACTCATCCAAAAGCCAATGCCAACACTTCAGGTTTTTTACCTATAGAAAGAGCTTGGATTGATGATTTGCTAAA
GCTTGGTTTTATAGATACTTTTAGAGAAATTAATGGCGAAATCAAAGAAAAATACTCGTGGTGGAGCTATAGAATGAAAG
CAAGAGAAAGAAATGTAGGCTGGAGGATTGATTATTTTTTCATTTCAAAGGGTTTAAAAGATAAACTTAAAAACGCTTTC
ATAAGAGATGATATTTTTGGCTCTGATCACGCACCTGTAGGTATAGAAATAGATATTTAA

Upstream 100 bases:

>100_bases
GCATATAAATCCCACCTTCAAATTTAAGCTTTTAATTTTATTGAATTTTTTTAAATGGATTGTAGTTTTAAATCTTTTTA
TGTTATGATTTACACTTTTA

Downstream 100 bases:

>100_bases
TATCCCAAAAATGGGATATTATTTTTTAACGAATTTTTTTGCTTTTTCCCAAAGTCTTACACCGAGCTTTTTAGCTTTTT
TGGAATTTAAATCTTTGTAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKILRKIMKLLSWNVNGLRAICDKNALDWIAQEQIDFIGFQEIKAHEDKFPKKIYEYPFKHMYFNSAKRAGYSGVMSLCN
FNSEVKKCEFFDDEEGRVLEHRFKNIALFNIYFPNGQKDEERLNFKMQFYADFLVYLDKLLKDGFEIIICGDVNTAHKEI
DLTHPKANANTSGFLPIERAWIDDLLKLGFIDTFREINGEIKEKYSWWSYRMKARERNVGWRIDYFFISKGLKDKLKNAF
IRDDIFGSDHAPVGIEIDI

Sequences:

>Translated_259_residues
MKILRKIMKLLSWNVNGLRAICDKNALDWIAQEQIDFIGFQEIKAHEDKFPKKIYEYPFKHMYFNSAKRAGYSGVMSLCN
FNSEVKKCEFFDDEEGRVLEHRFKNIALFNIYFPNGQKDEERLNFKMQFYADFLVYLDKLLKDGFEIIICGDVNTAHKEI
DLTHPKANANTSGFLPIERAWIDDLLKLGFIDTFREINGEIKEKYSWWSYRMKARERNVGWRIDYFFISKGLKDKLKNAF
IRDDIFGSDHAPVGIEIDI
>Mature_259_residues
MKILRKIMKLLSWNVNGLRAICDKNALDWIAQEQIDFIGFQEIKAHEDKFPKKIYEYPFKHMYFNSAKRAGYSGVMSLCN
FNSEVKKCEFFDDEEGRVLEHRFKNIALFNIYFPNGQKDEERLNFKMQFYADFLVYLDKLLKDGFEIIICGDVNTAHKEI
DLTHPKANANTSGFLPIERAWIDDLLKLGFIDTFREINGEIKEKYSWWSYRMKARERNVGWRIDYFFISKGLKDKLKNAF
IRDDIFGSDHAPVGIEIDI

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=37.2549019607843, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=37.2549019607843, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=37.2549019607843, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI18375507, Length=316, Percent_Identity=29.746835443038, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=32.7067669172932, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=34.9809885931559, Blast_Score=143, Evalue=7e-35,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=37.1541501976285, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=37.1541501976285, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30634; Mature: 30634

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILRKIMKLLSWNVNGLRAICDKNALDWIAQEQIDFIGFQEIKAHEDKFPKKIYEYPFK
CHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHCCHH
HMYFNSAKRAGYSGVMSLCNFNSEVKKCEFFDDEEGRVLEHRFKNIALFNIYFPNGQKDE
HHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCH
ERLNFKMQFYADFLVYLDKLLKDGFEIIICGDVNTAHKEIDLTHPKANANTSGFLPIERA
HHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHEEEECCCCCCCCCCCCCCHHHH
WIDDLLKLGFIDTFREINGEIKEKYSWWSYRMKARERNVGWRIDYFFISKGLKDKLKNAF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHH
IRDDIFGSDHAPVGIEIDI
HHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKILRKIMKLLSWNVNGLRAICDKNALDWIAQEQIDFIGFQEIKAHEDKFPKKIYEYPFK
CHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHCCHH
HMYFNSAKRAGYSGVMSLCNFNSEVKKCEFFDDEEGRVLEHRFKNIALFNIYFPNGQKDE
HHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCH
ERLNFKMQFYADFLVYLDKLLKDGFEIIICGDVNTAHKEIDLTHPKANANTSGFLPIERA
HHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHEEEECCCCCCCCCCCCCCHHHH
WIDDLLKLGFIDTFREINGEIKEKYSWWSYRMKARERNVGWRIDYFFISKGLKDKLKNAF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHH
IRDDIFGSDHAPVGIEIDI
HHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]