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The map label for this gene is proP [H]

Identifier: 57237307

GI number: 57237307

Start: 264966

End: 266276

Strand: Reverse

Name: proP [H]

Synonym: CJE0300

Alternate gene names: 57237307

Gene position: 266276-264966 (Counterclockwise)

Preceding gene: 57237308

Following gene: 57237304

Centisome position: 14.98

GC content: 29.6

Gene sequence:

>1311_bases
ATGAACAAAACCCTTAACCAAAAAGATATAAAAGTTTTAGGACTTTCATCATTAGGTGGAACTTTAGAATTTTATGATTT
TATTATTTTTGTATTTTTTGCAAATTATATTTCAACAAACTTTTTTCCAAAAGATTTAAGTAGTTTTTGGCAAATGTTTA
ATACTTATGGAATATTTGCAGCTGGATATTTAGCGCGTCCCTTAGGGGGTGTGATACTGGCACATTTTGGAGATAAATTT
GGACGCAAAAGAATGTTTATGATTAGTATTTTGTTAATGGTTATTCCAACTTTTACTCTAGCTTTTATTCCAAATTATGA
AAGTATAGGTTTTCTTTGTATTGTGCTTTTAGTTTTTATACGCATTTGTCAAGGTATAGCTATAGGCGGGGAACTTCCTG
GAGCTTGGGTTTTTGTATACGAACACGCACCACAAGGTCAAAAAAGAACTTATTTGGGAATTTTAACCGCTTCGGTTGTA
GGTGGAATTTTATTAGGTAGTTTAGTTTTTTTAATTATGAATAAAATTTATACCCAAGAAGAACTTCATGAATGGGCTTG
GAGAATTCCATTCTTTTTAGGTGGAATTTTTGGGATTATTTCTGCTTATTTGAGAAAATTTTTAAGAGAAACTCCTGTTT
TTGAACAAATGAAAAAAGACAAGGCTTTAGAAAAATTTCCTTTAAAAGAAGTGTTTAAAAAAGCTAAAATGGGAATTGTT
CTTTCTATGATGATTACTTGGGTTTTGACAGGTTGTATTGTTGTAATGATTTTGCTTATGCCTAGTTATATGGCAAAAAT
ACTTCAAATTAATACTTCTATACAAACCTATCTTCAAATAGGTGGAATTTTGCTTATTTGCCTAGGGTGTATTATAAGTG
GAATTTTAGCGGATAAAATAAGCGTTATTAAATCTTGTGTATTTTTTAGTGTTTTTTTTGGAATTGCGAGCTTGCTTTAT
TTTAATACTTTATATAGACAAAATGCTGATTTTAACCTAGTAGCTTGCTTATATTTACTTGTATGTTTTTTTAGCGGGGT
GATGAATTTTTGCCCTTTAATAATGAGTGAAGTATTTGATGCAAAAATTAAATTTTCAGGACTTTCTTTTTCATATAATA
TTGCTTATGCTATAGCTGGAGGTTTAACTCCGCAACTTGCTTTTTTCTTGCATAGTTTTGCCTTAAATAATTTAAGTAAT
TTTTGGCGTTTTTCTTTGGGACTTTATGTTTTCTTTTTGGCTATTATAGCTTTGCTATGTACATTTATTTTTTCTTATCT
TAATAATACCCAACGCACATACTCTCAGTGA

Upstream 100 bases:

>100_bases
TATACACAAAATCACCATAAAAATTATAATTATGATGATGATGATTATGAATACGATGATGATAACAATGATGATGATTT
TTATGAGATGGAGATTAAGA

Downstream 100 bases:

>100_bases
TTTTAATACGCTTTTCAAAAGGAGCTGGGCTAAAAAAATGGCATTTTTCTATATAAATATGCTGATGATAATTTAGCCCT
AAAAAATCATAAAATTTTTT

Product: major facilitator superfamily protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNKTLNQKDIKVLGLSSLGGTLEFYDFIIFVFFANYISTNFFPKDLSSFWQMFNTYGIFAAGYLARPLGGVILAHFGDKF
GRKRMFMISILLMVIPTFTLAFIPNYESIGFLCIVLLVFIRICQGIAIGGELPGAWVFVYEHAPQGQKRTYLGILTASVV
GGILLGSLVFLIMNKIYTQEELHEWAWRIPFFLGGIFGIISAYLRKFLRETPVFEQMKKDKALEKFPLKEVFKKAKMGIV
LSMMITWVLTGCIVVMILLMPSYMAKILQINTSIQTYLQIGGILLICLGCIISGILADKISVIKSCVFFSVFFGIASLLY
FNTLYRQNADFNLVACLYLLVCFFSGVMNFCPLIMSEVFDAKIKFSGLSFSYNIAYAIAGGLTPQLAFFLHSFALNNLSN
FWRFSLGLYVFFLAIIALLCTFIFSYLNNTQRTYSQ

Sequences:

>Translated_436_residues
MNKTLNQKDIKVLGLSSLGGTLEFYDFIIFVFFANYISTNFFPKDLSSFWQMFNTYGIFAAGYLARPLGGVILAHFGDKF
GRKRMFMISILLMVIPTFTLAFIPNYESIGFLCIVLLVFIRICQGIAIGGELPGAWVFVYEHAPQGQKRTYLGILTASVV
GGILLGSLVFLIMNKIYTQEELHEWAWRIPFFLGGIFGIISAYLRKFLRETPVFEQMKKDKALEKFPLKEVFKKAKMGIV
LSMMITWVLTGCIVVMILLMPSYMAKILQINTSIQTYLQIGGILLICLGCIISGILADKISVIKSCVFFSVFFGIASLLY
FNTLYRQNADFNLVACLYLLVCFFSGVMNFCPLIMSEVFDAKIKFSGLSFSYNIAYAIAGGLTPQLAFFLHSFALNNLSN
FWRFSLGLYVFFLAIIALLCTFIFSYLNNTQRTYSQ
>Mature_436_residues
MNKTLNQKDIKVLGLSSLGGTLEFYDFIIFVFFANYISTNFFPKDLSSFWQMFNTYGIFAAGYLARPLGGVILAHFGDKF
GRKRMFMISILLMVIPTFTLAFIPNYESIGFLCIVLLVFIRICQGIAIGGELPGAWVFVYEHAPQGQKRTYLGILTASVV
GGILLGSLVFLIMNKIYTQEELHEWAWRIPFFLGGIFGIISAYLRKFLRETPVFEQMKKDKALEKFPLKEVFKKAKMGIV
LSMMITWVLTGCIVVMILLMPSYMAKILQINTSIQTYLQIGGILLICLGCIISGILADKISVIKSCVFFSVFFGIASLLY
FNTLYRQNADFNLVACLYLLVCFFSGVMNFCPLIMSEVFDAKIKFSGLSFSYNIAYAIAGGLTPQLAFFLHSFALNNLSN
FWRFSLGLYVFFLAIIALLCTFIFSYLNNTQRTYSQ

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=30.8252427184466, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1788942, Length=439, Percent_Identity=27.5626423690205, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=26.2068965517241, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=28.8056206088993, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082404, Length=421, Percent_Identity=23.0403800475059, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=437, Percent_Identity=19.6796338672769, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49301; Mature: 49301

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCHHHCCC
>Mature Secondary Structure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HHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]