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The map label for this gene is purN [H]

Identifier: 57237192

GI number: 57237192

Start: 180887

End: 181453

Strand: Reverse

Name: purN [H]

Synonym: CJE0180

Alternate gene names: 57237192

Gene position: 181453-180887 (Counterclockwise)

Preceding gene: 57237193

Following gene: 57237191

Centisome position: 10.21

GC content: 29.28

Gene sequence:

>567_bases
ATGCTTGTAAAATTAGCTGTACTTTTTAGTGGAAATGGAAGTAATTTAGAAAATATTTTAGAAAAACTTCATAAAAAAAC
CATAGGGGAAAATACCTATGAAGTAGTACTTTGCATTTGTAATAAAAAAGATGCTTTTGGTGTACAAAGAGCAAAAAAAT
TTGGTCTTGATACTGTCATTGTTGATCATAAGGCTTATAATACTCGTGAAGAATTTGATACGATTTTGGTACAAAAAATC
AAAGAAAGTGGAGCAAATCTTACTGTATTAGCAGGTTTTATGAGAATTTTAAGTCCTGTTTTTACAAAAAATATTAAGGC
AATTAACCTTCATCCTTCTTTATTACCCCTTTTTAAAGGAGCACATGCGATTAAAGAAAGTTATGAAAGTGATATGAAAG
TGGCTGGAGTCAGTGTGCATTGGGTCAGTGAAGAACTTGATAGTGGAATGATTATAGCTCAAAAAGCTTTTGAAAAAAGA
AATTTGAGTTTTGAAGAATTTGAAGAAAAAATTCATAGTCTAGAACATGAAATTTTACCCTTAAGTGTGATTGAAATTTT
TTCTTAA

Upstream 100 bases:

>100_bases
AAGCAGCAAAAAATGCTACTTTAGCTCATGGACTTGTTGCAAAAAAATATAAATTTAATAAAAATAGCTTTGACGCTTTA
AAACTTATCAAAGGATTAAA

Downstream 100 bases:

>100_bases
AGAATTAAAGGTATTTGCTTTAAAACTATAAAATATCGCTTTAAAAAATTTTAAAATTTTCAAGGAAATTTATGTTTGAA
TGGATATTTAGTATTGATGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MLVKLAVLFSGNGSNLENILEKLHKKTIGENTYEVVLCICNKKDAFGVQRAKKFGLDTVIVDHKAYNTREEFDTILVQKI
KESGANLTVLAGFMRILSPVFTKNIKAINLHPSLLPLFKGAHAIKESYESDMKVAGVSVHWVSEELDSGMIIAQKAFEKR
NLSFEEFEEKIHSLEHEILPLSVIEIFS

Sequences:

>Translated_188_residues
MLVKLAVLFSGNGSNLENILEKLHKKTIGENTYEVVLCICNKKDAFGVQRAKKFGLDTVIVDHKAYNTREEFDTILVQKI
KESGANLTVLAGFMRILSPVFTKNIKAINLHPSLLPLFKGAHAIKESYESDMKVAGVSVHWVSEELDSGMIIAQKAFEKR
NLSFEEFEEKIHSLEHEILPLSVIEIFS
>Mature_188_residues
MLVKLAVLFSGNGSNLENILEKLHKKTIGENTYEVVLCICNKKDAFGVQRAKKFGLDTVIVDHKAYNTREEFDTILVQKI
KESGANLTVLAGFMRILSPVFTKNIKAINLHPSLLPLFKGAHAIKESYESDMKVAGVSVHWVSEELDSGMIIAQKAFEKR
NLSFEEFEEKIHSLEHEILPLSVIEIFS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=36.4130434782609, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=36.4130434782609, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=36.4130434782609, Blast_Score=128, Evalue=4e-30,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=38.0952380952381, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1787483, Length=105, Percent_Identity=36.1904761904762, Blast_Score=71, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=39.0374331550802, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=27.6381909547739, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=37.2222222222222, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21146; Mature: 21146

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVKLAVLFSGNGSNLENILEKLHKKTIGENTYEVVLCICNKKDAFGVQRAKKFGLDTVI
CEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
VDHKAYNTREEFDTILVQKIKESGANLTVLAGFMRILSPVFTKNIKAINLHPSLLPLFKG
ECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
AHAIKESYESDMKVAGVSVHWVSEELDSGMIIAQKAFEKRNLSFEEFEEKIHSLEHEILP
HHHHHHHHHCCCEEECEEEEEEHHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCH
LSVIEIFS
HHHHHHCC
>Mature Secondary Structure
MLVKLAVLFSGNGSNLENILEKLHKKTIGENTYEVVLCICNKKDAFGVQRAKKFGLDTVI
CEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
VDHKAYNTREEFDTILVQKIKESGANLTVLAGFMRILSPVFTKNIKAINLHPSLLPLFKG
ECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
AHAIKESYESDMKVAGVSVHWVSEELDSGMIIAQKAFEKRNLSFEEFEEKIHSLEHEILP
HHHHHHHHHCCCEEECEEEEEEHHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCH
LSVIEIFS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]