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The map label for this gene is mcrB [H]

Identifier: 57237146

GI number: 57237146

Start: 139354

End: 141342

Strand: Direct

Name: mcrB [H]

Synonym: CJE0134

Alternate gene names: 57237146

Gene position: 139354-141342 (Clockwise)

Preceding gene: 57237145

Following gene: 57237147

Centisome position: 7.84

GC content: 25.19

Gene sequence:

>1989_bases
ATGAATATTGCTGAATTTCATTTTTCAAATGGAAATAAAATACAAGCACAAATAGAAGATTCAAATGAGAAAATTTTTAA
GATATTTTTGGAAAAAAGACTAAAAAATTACAATCAATATATCAAAGCTACAAAAGACATAATTAAAGAATGTAAAAGAT
TAAATATTGTCAATAGAGATGATTTTGATACGTTTCAAATAAATTCTATAGAAGTTTTAACAAATTTGTTATTTGAATTA
AAAAATGGGCTATTGCGAGAATATGATCTTAAGAGCAGCAATCGATGTCCGCATGCTTGTATTAAATCTCATTATTTGGA
ATTTGTAAAATATGTCAATGCTTATAAAAATTATGTTTCAAAAGATATGGTTAAGTATTTGAAGACAAGAGGTGGTGAGA
AAGAATTTAAAGTAGAAATAAAAAATCAAAAGATAGAAATTATTGCTAGTATTAATAATATTAGAAAATACTCTTCGAAT
AGATTTTTGGAATATAGTGTTTTAGGTAAAAATGAAGTTGAGTCATATGCTAAACCATTATTTGAAGATATATTAAGCAA
CTCAAATCTTAGTGCGATTAAGCAAAGCGAAAATGAAAAGATACTAATTGAAAATCAAAATATACCTTTAAATCAAATTC
TTTATGGGCCTCCAGGGACAGGAAAAACTTATCATACTATAGATAAAGCTTTAGAAATTTTAGGTGAAAATTTAGAAAAC
AGAGATGAGAAAAAAGCAAAATTTGATGAGTATGTAAAAAATGGACAAATAGTTTTTACCACTTTTCATCAAAGCTATGG
ATATGAAGAATTTGTAGAAGGCATAAAGCCTCGCATAGATAGCAAAGAAAATTCTAAGGAAGTAGAATACGAAATAAAAG
ACGGAATTTTTAAAGAACTTTGTAAAAAAGCTTTGGATAATTACAAAGTATCTTTATTAACACAAGAAGAATTTGTAAAG
AGTGAAGATTTAGAAAACAAAATAGAAATTTTTTTAGATGAATTAGTAGATCAACAAAAATTTATAGAAAAAATTCAAAG
TGGTGGATTTAAATTAGAAGAATATAATGAAAAATATAGAATTATCACAGATGATACTAATGCAAATTTGTATTTAAATT
TAGAAATTTTTAAAACGCTTTTGGAAAATAAAGATAAGATTATAAACGGAAGAAGCATTAAGCAAATTTTAAATAATAAA
CATAGAAGACAAATCGATAGTTACTATTTTCAGCTAGTTAAATTGTTTAAAGAACGAGAACAAGATTATAAAGTAGATAA
TAATCCTAGTGAAAAGCCAGAATTAAAACCTTATATAATCATCATCGATGAAATCAATCGTGGTAATGTAAGTAAGATTT
TTGGTGAGCTTATAACCTTGATAGAACCTAGTAAAAGGATAGGTGAAAAAGAAGAGTTAAAAGTGACTTTGCCTTATAGC
GGTGAAAAATTTGGAGTGCCTAAAAATGTTTATATCATAGGCACTATGAATACAGCCGATAGAAGCATAACTTCTCTTGA
TACAGCTTTAAGAAGAAGATTTGAATTTATTGAGATGATGCCTGATGTGAGCAAATTATCTATGGATTGCGAAGGTATTA
ATTTACAAGAATTATTAAAAGCTATAAACACTCGTATAGAATATTTACTAGATAGAGAAAAAACCATAGGTCATGCATTT
TTTGTAAGTGTTGAAAATTTAGAAGATTTAAAAAAAGTTTTTCAAAATAAAATTATTCCGCTTTTGCAAGAGTATTTTTA
TAATGATTATGCTTTGATTAATGAGGTTTTAAATGATAATGGTATGATTTTTGAAGATAAAAAAGATGATAAATATCTTC
AAAAAATAAAAAATCTAGATAGTGTTAATAGCGAGAGAAGTATTTATAATATTGCTTCATTTGATGATAAAATTTGGGAT
AAAATAGAAATATATCAAGCAATTTATAATGATGAAATAGCAAATAAATTAAAAAACGAAAATGAATAG

Upstream 100 bases:

>100_bases
TTAGAAATATGATTTTTTAATTACCTTTTATATAATTAAATTTAAATATAAACACAAAAAACTATAAAATTTTATATTTG
TAGGTTAAAAGGAAAATAGT

Downstream 100 bases:

>100_bases
CACTTTTTCCATCATCGAATACCAAGCTTTTTCTAAAGAAGATTTAAAAGAAATTTTCAAAGAAAAGGCCGAAGGGTTTT
ATAAAGAGCTAGAAGACTTT

Product: McrBC restriction endonuclease system, McrB subunit

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MNIAEFHFSNGNKIQAQIEDSNEKIFKIFLEKRLKNYNQYIKATKDIIKECKRLNIVNRDDFDTFQINSIEVLTNLLFEL
KNGLLREYDLKSSNRCPHACIKSHYLEFVKYVNAYKNYVSKDMVKYLKTRGGEKEFKVEIKNQKIEIIASINNIRKYSSN
RFLEYSVLGKNEVESYAKPLFEDILSNSNLSAIKQSENEKILIENQNIPLNQILYGPPGTGKTYHTIDKALEILGENLEN
RDEKKAKFDEYVKNGQIVFTTFHQSYGYEEFVEGIKPRIDSKENSKEVEYEIKDGIFKELCKKALDNYKVSLLTQEEFVK
SEDLENKIEIFLDELVDQQKFIEKIQSGGFKLEEYNEKYRIITDDTNANLYLNLEIFKTLLENKDKIINGRSIKQILNNK
HRRQIDSYYFQLVKLFKEREQDYKVDNNPSEKPELKPYIIIIDEINRGNVSKIFGELITLIEPSKRIGEKEELKVTLPYS
GEKFGVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINTRIEYLLDREKTIGHAF
FVSVENLEDLKKVFQNKIIPLLQEYFYNDYALINEVLNDNGMIFEDKKDDKYLQKIKNLDSVNSERSIYNIASFDDKIWD
KIEIYQAIYNDEIANKLKNENE

Sequences:

>Translated_662_residues
MNIAEFHFSNGNKIQAQIEDSNEKIFKIFLEKRLKNYNQYIKATKDIIKECKRLNIVNRDDFDTFQINSIEVLTNLLFEL
KNGLLREYDLKSSNRCPHACIKSHYLEFVKYVNAYKNYVSKDMVKYLKTRGGEKEFKVEIKNQKIEIIASINNIRKYSSN
RFLEYSVLGKNEVESYAKPLFEDILSNSNLSAIKQSENEKILIENQNIPLNQILYGPPGTGKTYHTIDKALEILGENLEN
RDEKKAKFDEYVKNGQIVFTTFHQSYGYEEFVEGIKPRIDSKENSKEVEYEIKDGIFKELCKKALDNYKVSLLTQEEFVK
SEDLENKIEIFLDELVDQQKFIEKIQSGGFKLEEYNEKYRIITDDTNANLYLNLEIFKTLLENKDKIINGRSIKQILNNK
HRRQIDSYYFQLVKLFKEREQDYKVDNNPSEKPELKPYIIIIDEINRGNVSKIFGELITLIEPSKRIGEKEELKVTLPYS
GEKFGVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINTRIEYLLDREKTIGHAF
FVSVENLEDLKKVFQNKIIPLLQEYFYNDYALINEVLNDNGMIFEDKKDDKYLQKIKNLDSVNSERSIYNIASFDDKIWD
KIEIYQAIYNDEIANKLKNENE
>Mature_662_residues
MNIAEFHFSNGNKIQAQIEDSNEKIFKIFLEKRLKNYNQYIKATKDIIKECKRLNIVNRDDFDTFQINSIEVLTNLLFEL
KNGLLREYDLKSSNRCPHACIKSHYLEFVKYVNAYKNYVSKDMVKYLKTRGGEKEFKVEIKNQKIEIIASINNIRKYSSN
RFLEYSVLGKNEVESYAKPLFEDILSNSNLSAIKQSENEKILIENQNIPLNQILYGPPGTGKTYHTIDKALEILGENLEN
RDEKKAKFDEYVKNGQIVFTTFHQSYGYEEFVEGIKPRIDSKENSKEVEYEIKDGIFKELCKKALDNYKVSLLTQEEFVK
SEDLENKIEIFLDELVDQQKFIEKIQSGGFKLEEYNEKYRIITDDTNANLYLNLEIFKTLLENKDKIINGRSIKQILNNK
HRRQIDSYYFQLVKLFKEREQDYKVDNNPSEKPELKPYIIIIDEINRGNVSKIFGELITLIEPSKRIGEKEELKVTLPYS
GEKFGVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINTRIEYLLDREKTIGHAF
FVSVENLEDLKKVFQNKIIPLLQEYFYNDYALINEVLNDNGMIFEDKKDDKYLQKIKNLDSVNSERSIYNIASFDDKIWD
KIEIYQAIYNDEIANKLKNENE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=183, Percent_Identity=41.5300546448087, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 77857; Mature: 77857

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAEFHFSNGNKIQAQIEDSNEKIFKIFLEKRLKNYNQYIKATKDIIKECKRLNIVNRD
CCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFDTFQINSIEVLTNLLFELKNGLLREYDLKSSNRCPHACIKSHYLEFVKYVNAYKNYVS
CCCCEECCHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDMVKYLKTRGGEKEFKVEIKNQKIEIIASINNIRKYSSNRFLEYSVLGKNEVESYAKPL
HHHHHHHHHCCCCEEEEEEECCCEEEEEEEHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
FEDILSNSNLSAIKQSENEKILIENQNIPLNQILYGPPGTGKTYHTIDKALEILGENLEN
HHHHHCCCCCHHHHCCCCCEEEEECCCCCHHHEEECCCCCCCHHHHHHHHHHHHHCCCCC
RDEKKAKFDEYVKNGQIVFTTFHQSYGYEEFVEGIKPRIDSKENSKEVEYEIKDGIFKEL
CHHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
CKKALDNYKVSLLTQEEFVKSEDLENKIEIFLDELVDQQKFIEKIQSGGFKLEEYNEKYR
HHHHHCCCEEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
IITDDTNANLYLNLEIFKTLLENKDKIINGRSIKQILNNKHRRQIDSYYFQLVKLFKERE
EEEECCCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QDYKVDNNPSEKPELKPYIIIIDEINRGNVSKIFGELITLIEPSKRIGEKEELKVTLPYS
HCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCEEEECCCC
GEKFGVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
AINTRIEYLLDREKTIGHAFFVSVENLEDLKKVFQNKIIPLLQEYFYNDYALINEVLNDN
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
GMIFEDKKDDKYLQKIKNLDSVNSERSIYNIASFDDKIWDKIEIYQAIYNDEIANKLKNE
CCEEECCCCHHHHHHHHHHHCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
NE
CC
>Mature Secondary Structure
MNIAEFHFSNGNKIQAQIEDSNEKIFKIFLEKRLKNYNQYIKATKDIIKECKRLNIVNRD
CCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFDTFQINSIEVLTNLLFELKNGLLREYDLKSSNRCPHACIKSHYLEFVKYVNAYKNYVS
CCCCEECCHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDMVKYLKTRGGEKEFKVEIKNQKIEIIASINNIRKYSSNRFLEYSVLGKNEVESYAKPL
HHHHHHHHHCCCCEEEEEEECCCEEEEEEEHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
FEDILSNSNLSAIKQSENEKILIENQNIPLNQILYGPPGTGKTYHTIDKALEILGENLEN
HHHHHCCCCCHHHHCCCCCEEEEECCCCCHHHEEECCCCCCCHHHHHHHHHHHHHCCCCC
RDEKKAKFDEYVKNGQIVFTTFHQSYGYEEFVEGIKPRIDSKENSKEVEYEIKDGIFKEL
CHHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
CKKALDNYKVSLLTQEEFVKSEDLENKIEIFLDELVDQQKFIEKIQSGGFKLEEYNEKYR
HHHHHCCCEEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
IITDDTNANLYLNLEIFKTLLENKDKIINGRSIKQILNNKHRRQIDSYYFQLVKLFKERE
EEEECCCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QDYKVDNNPSEKPELKPYIIIIDEINRGNVSKIFGELITLIEPSKRIGEKEELKVTLPYS
HCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCEEEECCCC
GEKFGVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
AINTRIEYLLDREKTIGHAFFVSVENLEDLKKVFQNKIIPLLQEYFYNDYALINEVLNDN
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
GMIFEDKKDDKYLQKIKNLDSVNSERSIYNIASFDDKIWDKIEIYQAIYNDEIANKLKNE
CCEEECCCCHHHHHHHHHHHCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
NE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]