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The map label for this gene is glnA [H]

Identifier: 57237002

GI number: 57237002

Start: 735639

End: 737069

Strand: Reverse

Name: glnA [H]

Synonym: CJE0798

Alternate gene names: 57237002

Gene position: 737069-735639 (Counterclockwise)

Preceding gene: 57238721

Following gene: 57236996

Centisome position: 41.46

GC content: 33.19

Gene sequence:

>1431_bases
ATGGGAAAATTTGTTAATAATATAGATGATTTTTTCAAATTTTGCAAACAAAATGAAGTGCTTTTTGTTGATTTTCGTTT
TACAGATATGATAGGAACTTGGCATCATATTACCTATAATCTTCATGCAATCAATGAAGAAACATTTCAAACTGGCATTC
CATTTGATGGAAGCTCAATTCATGGATGGCAACCTATAGAAAAATCAGATATGATTTTAAAACCTGATGCTCAAAGTGCT
TTTTTAGATCCTTTTACGGCTGATCCTACTATCATAGTATTTTGTGATGTGTATGATATTTACAAAGGACAAATGTATGA
AAAATGTCCAAGAAGCATAGCAAAAAAAGCAATGGAACACCTTAAAAATAGTGGCATAGCTGATACTGCTTACTTTGGAC
CAGAAAATGAATTCTTTGTTTTTGATAGTGTAAAAATAGTTGATACTGCTCATTGTTCTAAGTATGAAGTTGATACCGAA
GAAGGAGAGTGGAATGATGATAGAGAATTTACCGATAGCTACAATACTGGACACAGGCCAAGAAACAAAGGTGGATATTT
TCCAGTTCAGCCAATTGATTCTTTAGTAGATATTCGTTCTGAAATGGTTCAAACCCTTGAAAAAGTAGGTCTTAAAACTT
TTGTTCATCATCATGAAGTTGCACAAGGACAAGCTGAAATAGGAGTAAATTTTGGCACGCTTGTAGAAGCAGCTGACAAT
GTTCAAATTTATAAATATGTTGTAAAAATGGTAGCTCATTTAAATGGCAAAACTGCCACTTTTATGCCAAAACCTTTATA
CGGAGATAATGGCAATGGTATGCATGTTCATATGAGCTTATGGAAAAATGGAGTAAATTTATTTTACGATAAAGATGGAT
ACAGTGGACTTAGTCAAACTGCTATAAATTATATAGGTGGAATTTTAAAAAATGCTAGAAGTGTTGCAGCTTTTACAAAT
CCAAGTTCCAACTCTTACAAAAGAATAGTTCCTGGCTTTGAAGCTCCTTGTATTTTAACTTATTCTTGTCAAAACAGATC
AGCAAGTTGTCGCGTTCCTTATGGCATAGGTAAAAATTCAGCTCGTATAGAAATTCGCTTCCCTGATAGCACTGCTAATC
CTTATTTAGCTTTTGTTAGTCTTTTAATGGCAGGTCTTGATGGAATCAAAAATAAAACCATTCCTGTGGGTCCTATGGAT
GAAAATTTATTTGATCTTACTTTGGATGAAATACGTGAAAAAGGGATAGAGCAATTACCACATACTCTAAGAGGAAGTTT
AGAAGCTTTAATCCGACATAATTCTTATTTAAAACCTGTAATGAGCGATATTTTTATCGATGACTATCAGCATTTAAAAT
TTGAAACACAAGTTTGGCCAGTAGAAGCTCGTCCAACTGCTTATGAATTTAAAACTTGCTACTCTTGCTAA

Upstream 100 bases:

>100_bases
TTAAATTTTTAAATTTATTAAAAAAAATATATTAATTAAATCTTAATTAAAAATTTTATGATATAATTTTATAAATTTAA
TTACATAAAGGAGTAAAAAT

Downstream 100 bases:

>100_bases
AAATTTAATGGCGTTTAACACGCCATTAAACAAAAAAGAATTAAAAATTATTTATTTTATATCAACTATCGCTTAAGCTG
ATTTACAATTCCTAGCATAT

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MGKFVNNIDDFFKFCKQNEVLFVDFRFTDMIGTWHHITYNLHAINEETFQTGIPFDGSSIHGWQPIEKSDMILKPDAQSA
FLDPFTADPTIIVFCDVYDIYKGQMYEKCPRSIAKKAMEHLKNSGIADTAYFGPENEFFVFDSVKIVDTAHCSKYEVDTE
EGEWNDDREFTDSYNTGHRPRNKGGYFPVQPIDSLVDIRSEMVQTLEKVGLKTFVHHHEVAQGQAEIGVNFGTLVEAADN
VQIYKYVVKMVAHLNGKTATFMPKPLYGDNGNGMHVHMSLWKNGVNLFYDKDGYSGLSQTAINYIGGILKNARSVAAFTN
PSSNSYKRIVPGFEAPCILTYSCQNRSASCRVPYGIGKNSARIEIRFPDSTANPYLAFVSLLMAGLDGIKNKTIPVGPMD
ENLFDLTLDEIREKGIEQLPHTLRGSLEALIRHNSYLKPVMSDIFIDDYQHLKFETQVWPVEARPTAYEFKTCYSC

Sequences:

>Translated_476_residues
MGKFVNNIDDFFKFCKQNEVLFVDFRFTDMIGTWHHITYNLHAINEETFQTGIPFDGSSIHGWQPIEKSDMILKPDAQSA
FLDPFTADPTIIVFCDVYDIYKGQMYEKCPRSIAKKAMEHLKNSGIADTAYFGPENEFFVFDSVKIVDTAHCSKYEVDTE
EGEWNDDREFTDSYNTGHRPRNKGGYFPVQPIDSLVDIRSEMVQTLEKVGLKTFVHHHEVAQGQAEIGVNFGTLVEAADN
VQIYKYVVKMVAHLNGKTATFMPKPLYGDNGNGMHVHMSLWKNGVNLFYDKDGYSGLSQTAINYIGGILKNARSVAAFTN
PSSNSYKRIVPGFEAPCILTYSCQNRSASCRVPYGIGKNSARIEIRFPDSTANPYLAFVSLLMAGLDGIKNKTIPVGPMD
ENLFDLTLDEIREKGIEQLPHTLRGSLEALIRHNSYLKPVMSDIFIDDYQHLKFETQVWPVEARPTAYEFKTCYSC
>Mature_475_residues
GKFVNNIDDFFKFCKQNEVLFVDFRFTDMIGTWHHITYNLHAINEETFQTGIPFDGSSIHGWQPIEKSDMILKPDAQSAF
LDPFTADPTIIVFCDVYDIYKGQMYEKCPRSIAKKAMEHLKNSGIADTAYFGPENEFFVFDSVKIVDTAHCSKYEVDTEE
GEWNDDREFTDSYNTGHRPRNKGGYFPVQPIDSLVDIRSEMVQTLEKVGLKTFVHHHEVAQGQAEIGVNFGTLVEAADNV
QIYKYVVKMVAHLNGKTATFMPKPLYGDNGNGMHVHMSLWKNGVNLFYDKDGYSGLSQTAINYIGGILKNARSVAAFTNP
SSNSYKRIVPGFEAPCILTYSCQNRSASCRVPYGIGKNSARIEIRFPDSTANPYLAFVSLLMAGLDGIKNKTIPVGPMDE
NLFDLTLDEIREKGIEQLPHTLRGSLEALIRHNSYLKPVMSDIFIDDYQHLKFETQVWPVEARPTAYEFKTCYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=50.8547008547009, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI87081870, Length=380, Percent_Identity=25.5263157894737, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53889; Mature: 53757

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKFVNNIDDFFKFCKQNEVLFVDFRFTDMIGTWHHITYNLHAINEETFQTGIPFDGSSI
CCCHHHHHHHHHHHHCCCCEEEEEEEEHHHCCCEEEEEEEEEECCHHHHHCCCCCCCCCC
HGWQPIEKSDMILKPDAQSAFLDPFTADPTIIVFCDVYDIYKGQMYEKCPRSIAKKAMEH
CCCCCCCCCCCEECCCCCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LKNSGIADTAYFGPENEFFVFDSVKIVDTAHCSKYEVDTEEGEWNDDREFTDSYNTGHRP
HHCCCCCCCEEECCCCCEEEEECEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
RNKGGYFPVQPIDSLVDIRSEMVQTLEKVGLKTFVHHHEVAQGQAEIGVNFGTLVEAADN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCC
VQIYKYVVKMVAHLNGKTATFMPKPLYGDNGNGMHVHMSLWKNGVNLFYDKDGYSGLSQT
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCCCHHHH
AINYIGGILKNARSVAAFTNPSSNSYKRIVPGFEAPCILTYSCQNRSASCRVPYGIGKNS
HHHHHHHHHHCCHHEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCCCEECCCCCCCCC
ARIEIRFPDSTANPYLAFVSLLMAGLDGIKNKTIPVGPMDENLFDLTLDEIREKGIEQLP
CEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
HTLRGSLEALIRHNSYLKPVMSDIFIDDYQHLKFETQVWPVEARPTAYEFKTCYSC
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCEEEEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure 
GKFVNNIDDFFKFCKQNEVLFVDFRFTDMIGTWHHITYNLHAINEETFQTGIPFDGSSI
CCHHHHHHHHHHHHCCCCEEEEEEEEHHHCCCEEEEEEEEEECCHHHHHCCCCCCCCCC
HGWQPIEKSDMILKPDAQSAFLDPFTADPTIIVFCDVYDIYKGQMYEKCPRSIAKKAMEH
CCCCCCCCCCCEECCCCCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LKNSGIADTAYFGPENEFFVFDSVKIVDTAHCSKYEVDTEEGEWNDDREFTDSYNTGHRP
HHCCCCCCCEEECCCCCEEEEECEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
RNKGGYFPVQPIDSLVDIRSEMVQTLEKVGLKTFVHHHEVAQGQAEIGVNFGTLVEAADN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCC
VQIYKYVVKMVAHLNGKTATFMPKPLYGDNGNGMHVHMSLWKNGVNLFYDKDGYSGLSQT
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCCCHHHH
AINYIGGILKNARSVAAFTNPSSNSYKRIVPGFEAPCILTYSCQNRSASCRVPYGIGKNS
HHHHHHHHHHCCHHEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCCCEECCCCCCCCC
ARIEIRFPDSTANPYLAFVSLLMAGLDGIKNKTIPVGPMDENLFDLTLDEIREKGIEQLP
CEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
HTLRGSLEALIRHNSYLKPVMSDIFIDDYQHLKFETQVWPVEARPTAYEFKTCYSC
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCEEEEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]