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The map label for this gene is mraW [H]

Identifier: 57236996

GI number: 57236996

Start: 728685

End: 729617

Strand: Reverse

Name: mraW [H]

Synonym: CJE0792

Alternate gene names: 57236996

Gene position: 729617-728685 (Counterclockwise)

Preceding gene: 57237002

Following gene: 57236995

Centisome position: 41.04

GC content: 28.19

Gene sequence:

>933_bases
TTGGAAATTCCTCATATCCCAGTTTTGCTTAATGAAGTTCAAGAGATTTTTAAAAATCTTAAAACAGGTTATTTTTTAGA
CTGCACTTTAGGATTTGGCGGACATAGTGAAGCTTTGCTTAAAAATCATCCTGATCTTAAATTCATTGCTTGTGATCAAG
ATCAACAAGCTTTAGAATTTTCTAAAAAACGTCTTAAAGATTTTCGCAATAGGATAACTTTTATGCAAAGTAATTTTAGT
GAAGTTTTAGAAAAAATTTCACATAAAGAAGAATTAAGAGGTATTTTAGCAGACATTGGAGTTTCTTCATTTCAGCTTGA
TAATAACGAAAGAGGTTTTAGTGTAAATTCTGACTTTTTGGATATGAGAATGAATCAAAACTCCAAAATATCGGCTTATG
AGATTATTAATACTTATACAAAAGAGCAATTAACTTCCATTTTTAAAGATTATGGAGAATTACATGATGCACATTTTATT
GCTGAAAAAATTTGCCTAGAAAGAAGTAAAAATCCAATCAAAAGTGCCAAAGAGCTTTATCAAATCATAGGAAAAGGTAA
ACAAAATCATAGAAAAATTTCCAAAGCTACTTTAGCATTTCAAGCCATTAGAATAGAAGTAAATCAAGAACTTAAAGTGC
TTAAAGATTTTCTTGGGCATTTAGAAAATTTAAAACCAAAAAATTGCATTTTAGCTATCATTAGCTTTCATTCTTTAGAA
GATAGAATAGTAAAACAATTTTTCAAAAAATGGTCAAAAAACTGCATTTGTAATGAAAAAATAATGCGTTGCGAATGTGG
AAATAACCATAGCCTAGGACAAATAATAACTAAAAAAGCTATAAGTGCGAGCAAGGAAGAACTTTTAAAAAATTCTCGCT
CTAGTTGTGCTAAAATGCGGGCTTTTTATTTTAATAATTTGGATAATAAATAA

Upstream 100 bases:

>100_bases
ATAAAATTTTTACCAAAAAATATTTGTTCTTTAAATTTTATGATTTTTACAATGAAATTTAGTTATAATTATAGTTTATA
AAAATTTATTAGGATTGATT

Downstream 100 bases:

>100_bases
TGCTAGAAGATGATTTTATAAAAGAACGCCAAAACATACGCCAAAAAATGCTGAAATTTTCTCGCGCCATAAATCAAGGC
AAACCGCTTGATGATGATTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSEALLKNHPDLKFIACDQDQQALEFSKKRLKDFRNRITFMQSNFS
EVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFLDMRMNQNSKISAYEIINTYTKEQLTSIFKDYGELHDAHFI
AEKICLERSKNPIKSAKELYQIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGHLENLKPKNCILAIISFHSLE
DRIVKQFFKKWSKNCICNEKIMRCECGNNHSLGQIITKKAISASKEELLKNSRSSCAKMRAFYFNNLDNK

Sequences:

>Translated_310_residues
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSEALLKNHPDLKFIACDQDQQALEFSKKRLKDFRNRITFMQSNFS
EVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFLDMRMNQNSKISAYEIINTYTKEQLTSIFKDYGELHDAHFI
AEKICLERSKNPIKSAKELYQIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGHLENLKPKNCILAIISFHSLE
DRIVKQFFKKWSKNCICNEKIMRCECGNNHSLGQIITKKAISASKEELLKNSRSSCAKMRAFYFNNLDNK
>Mature_310_residues
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSEALLKNHPDLKFIACDQDQQALEFSKKRLKDFRNRITFMQSNFS
EVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFLDMRMNQNSKISAYEIINTYTKEQLTSIFKDYGELHDAHFI
AEKICLERSKNPIKSAKELYQIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGHLENLKPKNCILAIISFHSLE
DRIVKQFFKKWSKNCICNEKIMRCECGNNHSLGQIITKKAISASKEELLKNSRSSCAKMRAFYFNNLDNK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=333, Percent_Identity=32.4324324324324, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=32.1608040201005, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=32.6860841423948, Blast_Score=144, Evalue=7e-36,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=30.3303303303303, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=30.3303303303303, Blast_Score=121, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35819; Mature: 35819

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSEALLKNHPDLKFIACDQDQQALEF
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCEEEECCCCHHHHHH
SKKRLKDFRNRITFMQSNFSEVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHH
DMRMNQNSKISAYEIINTYTKEQLTSIFKDYGELHDAHFIAEKICLERSKNPIKSAKELY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGHLENLKPKNCILAIISFHSLE
HHHHCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DRIVKQFFKKWSKNCICNEKIMRCECGNNHSLGQIITKKAISASKEELLKNSRSSCAKMR
HHHHHHHHHHHCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFYFNNLDNK
HHHHHCCCCC
>Mature Secondary Structure
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSEALLKNHPDLKFIACDQDQQALEF
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCEEEECCCCHHHHHH
SKKRLKDFRNRITFMQSNFSEVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHH
DMRMNQNSKISAYEIINTYTKEQLTSIFKDYGELHDAHFIAEKICLERSKNPIKSAKELY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGHLENLKPKNCILAIISFHSLE
HHHHCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DRIVKQFFKKWSKNCICNEKIMRCECGNNHSLGQIITKKAISASKEELLKNSRSSCAKMR
HHHHHHHHHHHCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFYFNNLDNK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]