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The map label for this gene is ackA [H]

Identifier: 57236992

GI number: 57236992

Start: 722148

End: 723338

Strand: Direct

Name: ackA [H]

Synonym: CJE0788

Alternate gene names: 57236992

Gene position: 722148-723338 (Clockwise)

Preceding gene: 57236991

Following gene: 57236994

Centisome position: 40.62

GC content: 32.33

Gene sequence:

>1191_bases
ATGAAAATTTTAGTTTTAAATTCAGGTTCTTCATCGATTAAATTTAAATTTTTTGATAATAAAATCGTTAAAGCCAGTGG
ATTGGTAGAAAAAATTGGAGAGCAAAATTCAAAGGTTGTATTAAAAAATACTTTAAATAACGAAAGTTTTGAAAGAGAAT
TAACAATAAATAATCACGAAGAAGGACTTAGTATAGTCAACGAGCTTTTTAAAGAATCAGGAATTTTAGCAGATTTAAAT
GCTCTTGATGGCTGTGGTCATCGTATAGTTCACGGGGGAAGAAATTTAAGTGAGCATTGTTTAGTAGATGATTATGTTCT
TAAAGAGATAGATAGGGTTAGTATTTTTGCTCCTTTGCATAATCCTGCCCATTTAGCAGGTATAAAAACAATGATCAAAG
CTGCACCTAGTGTTGCTAATGCAGCTATTTTTGATACAGCTTTTCATCGCACTATGCCTGATTTTGCTTATATGTATGCT
TTGCCTTATGATTTTTATGATAAGCATAATATTAGAAGATATGGTTTTCATGGGACTTCTCATGCTTATGTTAGTTCTAG
AGCGGCAAAATTTCTTCAAAAAGATCAAAATGAATTAAATGTCATCTCTGCTCATCTTGGAAATGGTGCAAGTGTATGTG
CTATAGAAAAAGGTAAGAGTATGGATACTTCTATGGGCTTTACTCCACTTGAAGGACTTATTATGGGAACGCGTTGTGGA
GATTTAGACCCTGCTATTTTACCTTTTGTTAGTCATCTTAAGGGTTTAACTATAGAAGAAATAGATACACTGATGAATAA
AAAAAGTGGAGTATATGGAATTTGCGGTTATAATGATTTTAGAGATATAGAAAGAGAGATAGAACAAGGTAATGATAAAG
CACGCTTGGCTCTTGATATGTTTTGTTATCGTCTTGTTAAATATATAGGATCCTACTTTGCTGTGTTGCCAAAAACCGAT
GCTATTATTTTTACAGGTGGTATAGGAGAAAATGATAGTTTGGTTCGACAAAAAGTATGCGAGAGATTAGCTCATCTTGG
TATCGAGTTAGATTTTGAACTTAACAAACAAAGGATTTCGGGCGAAAGAATGATAAATCATGCAAATTCTAAACTTAAAG
TGCTTGTTATTCCTACAGATGAAGAATTAGAAATCGCTAGAATCACAGAAGAACTTATCGGTAAAAATTAA

Upstream 100 bases:

>100_bases
AAAAAACCTGTTAATGATTTAAGTCGTGGATGTTTGGTAGATGATATTGTAGATACTGTTATTTTAAGTGCTATACAAGC
TCAATAGAAAGGAATAATAA

Downstream 100 bases:

>100_bases
ATTTTAAAAACTATATAAATCTTTCATTTATATAGTTTTTAGCAAGGTTGTATATTCACTTTGCTTTTCATTTAAAACTT
TAAAAGTGCTTATCATGTAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKILVLNSGSSSIKFKFFDNKIVKASGLVEKIGEQNSKVVLKNTLNNESFERELTINNHEEGLSIVNELFKESGILADLN
ALDGCGHRIVHGGRNLSEHCLVDDYVLKEIDRVSIFAPLHNPAHLAGIKTMIKAAPSVANAAIFDTAFHRTMPDFAYMYA
LPYDFYDKHNIRRYGFHGTSHAYVSSRAAKFLQKDQNELNVISAHLGNGASVCAIEKGKSMDTSMGFTPLEGLIMGTRCG
DLDPAILPFVSHLKGLTIEEIDTLMNKKSGVYGICGYNDFRDIEREIEQGNDKARLALDMFCYRLVKYIGSYFAVLPKTD
AIIFTGGIGENDSLVRQKVCERLAHLGIELDFELNKQRISGERMINHANSKLKVLVIPTDEELEIARITEELIGKN

Sequences:

>Translated_396_residues
MKILVLNSGSSSIKFKFFDNKIVKASGLVEKIGEQNSKVVLKNTLNNESFERELTINNHEEGLSIVNELFKESGILADLN
ALDGCGHRIVHGGRNLSEHCLVDDYVLKEIDRVSIFAPLHNPAHLAGIKTMIKAAPSVANAAIFDTAFHRTMPDFAYMYA
LPYDFYDKHNIRRYGFHGTSHAYVSSRAAKFLQKDQNELNVISAHLGNGASVCAIEKGKSMDTSMGFTPLEGLIMGTRCG
DLDPAILPFVSHLKGLTIEEIDTLMNKKSGVYGICGYNDFRDIEREIEQGNDKARLALDMFCYRLVKYIGSYFAVLPKTD
AIIFTGGIGENDSLVRQKVCERLAHLGIELDFELNKQRISGERMINHANSKLKVLVIPTDEELEIARITEELIGKN
>Mature_396_residues
MKILVLNSGSSSIKFKFFDNKIVKASGLVEKIGEQNSKVVLKNTLNNESFERELTINNHEEGLSIVNELFKESGILADLN
ALDGCGHRIVHGGRNLSEHCLVDDYVLKEIDRVSIFAPLHNPAHLAGIKTMIKAAPSVANAAIFDTAFHRTMPDFAYMYA
LPYDFYDKHNIRRYGFHGTSHAYVSSRAAKFLQKDQNELNVISAHLGNGASVCAIEKGKSMDTSMGFTPLEGLIMGTRCG
DLDPAILPFVSHLKGLTIEEIDTLMNKKSGVYGICGYNDFRDIEREIEQGNDKARLALDMFCYRLVKYIGSYFAVLPKTD
AIIFTGGIGENDSLVRQKVCERLAHLGIELDFELNKQRISGERMINHANSKLKVLVIPTDEELEIARITEELIGKN

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=45.75, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI145693184, Length=392, Percent_Identity=43.3673469387755, Blast_Score=311, Evalue=5e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 44118; Mature: 44118

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVLNSGSSSIKFKFFDNKIVKASGLVEKIGEQNSKVVLKNTLNNESFERELTINNHE
CEEEEEECCCCEEEEEEECCEEEHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCH
EGLSIVNELFKESGILADLNALDGCGHRIVHGGRNLSEHCLVDDYVLKEIDRVSIFAPLH
HHHHHHHHHHHHCCCEEECHHHCCCCHHHEECCCCCHHHHHHHHHHHHHHCCEEEEECCC
NPAHLAGIKTMIKAAPSVANAAIFDTAFHRTMPDFAYMYALPYDFYDKHNIRRYGFHGTS
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEECCCCCC
HAYVSSRAAKFLQKDQNELNVISAHLGNGASVCAIEKGKSMDTSMGFTPLEGLIMGTRCG
HHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCHHHEEEECCCC
DLDPAILPFVSHLKGLTIEEIDTLMNKKSGVYGICGYNDFRDIEREIEQGNDKARLALDM
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
FCYRLVKYIGSYFAVLPKTDAIIFTGGIGENDSLVRQKVCERLAHLGIELDFELNKQRIS
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHC
GERMINHANSKLKVLVIPTDEELEIARITEELIGKN
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVLNSGSSSIKFKFFDNKIVKASGLVEKIGEQNSKVVLKNTLNNESFERELTINNHE
CEEEEEECCCCEEEEEEECCEEEHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCH
EGLSIVNELFKESGILADLNALDGCGHRIVHGGRNLSEHCLVDDYVLKEIDRVSIFAPLH
HHHHHHHHHHHHCCCEEECHHHCCCCHHHEECCCCCHHHHHHHHHHHHHHCCEEEEECCC
NPAHLAGIKTMIKAAPSVANAAIFDTAFHRTMPDFAYMYALPYDFYDKHNIRRYGFHGTS
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEECCCCCC
HAYVSSRAAKFLQKDQNELNVISAHLGNGASVCAIEKGKSMDTSMGFTPLEGLIMGTRCG
HHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCHHHEEEECCCC
DLDPAILPFVSHLKGLTIEEIDTLMNKKSGVYGICGYNDFRDIEREIEQGNDKARLALDM
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
FCYRLVKYIGSYFAVLPKTDAIIFTGGIGENDSLVRQKVCERLAHLGIELDFELNKQRIS
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHC
GERMINHANSKLKVLVIPTDEELEIARITEELIGKN
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA