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The map label for this gene is ispG [H]

Identifier: 57236989

GI number: 57236989

Start: 718738

End: 719811

Strand: Direct

Name: ispG [H]

Synonym: CJE0785

Alternate gene names: 57236989

Gene position: 718738-719811 (Clockwise)

Preceding gene: 57236987

Following gene: 57236991

Centisome position: 40.43

GC content: 35.29

Gene sequence:

>1074_bases
ATGGAGTATAAAAGATTTAAAACCAGACAAATCAAAGTAGGAAATGTTTTAATAGGCGGTGATGCACCCATTTCAGTTCA
ATCTATGCTTTTTACTAAAACAAGAGATATAGAAGGTTCTTTAGAACAAATCAATCGTCTTTATTTTGCAGGAGCAAACA
TAGTGCGTTTGGCGTGCTTGGATATGGCTGATGCAAGGGCTTTAAAAGAGATTAAAGCTAAAAGTCCTCTACCTTTAATC
GTAGATATTCATTTTAATCATAATTTGGCTGTATATTGTGCTGAATTTATCGATGGCGTGCGTATAAATCCTGGCAATAT
AGGCTCTAAAGAAAATATTAAAGAAGTGGTAAAAGCATGTAAAGAACGGGGTATCCCTATACGCATCGGTATAAATCACG
GCTCCATAGAAAAACAATTTAGTGATAAATTTGGTTATGGTGTCGATGCCATGCTAGAAAGTGCGATGTATAATATCAAG
CTTTTAGAGGATTTAGACTTTTTTGATATTAAAATTTCCATGAAGACTTCAGATGCTCAAAAAACTATAGAAGCTTATGA
GAGATTAAGACCGCTTTGTGATTATCCTTTTCACTTAGGGGTAACTGAAGCAGGAACTAAATTTCATAGCACTGTAAAAA
GTTCTATAGCTTTAGGAAATTTACTTCTTAAAGGTATAGGCGATACTATGCGTGTTTCTATGACGGGTGAGCTTGAAGAA
GAAATTCGTGTAGCAAGAGCAATTTTGCAAGATAGCGGAGTACAAAAAAGCGGAGTAAATATTATTTCATGTCCAACTTG
CGGTAGGATTCAAAGTGATTTGTTAAGTGCGATTAAAATCGTAGAGGAAAAAACCAAGCATATAAAAGAACCTTTAAACA
TCAGTGTCATGGGTTGTGTGGTAAATGCTTTAGGCGAAGCCAAGGGCGCTGATGTGGCTATAGCCTTTGGGAAAAATCAA
GGACTTGTCATAAGACATGGTGAAGTTGTAGCTAAATTAAAAGAAAGTGAACTTGTAGATAGATTTTTAGCTGAAGTTGA
AGATGAGGTTAAAAGTAGGGCTGTAAAAGAATAA

Upstream 100 bases:

>100_bases
TTTAAATGTCATGAAATTTAAATATATTATCAAAAATAAACTTTAATTAAAGCAATAAAAAGTTAAAATTTGATATTAAA
ATTTTTCAAAGAGTGAAGTT

Downstream 100 bases:

>100_bases
AAGCCTAAAAGCAAGGTTTAACCTTGCTTTTAGAATGGCCATATCGCTTCTATAACTTTACTACCCCAAGGTTTTCTTGT
GCTTCTATCCACTTCTCCTT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEYKRFKTRQIKVGNVLIGGDAPISVQSMLFTKTRDIEGSLEQINRLYFAGANIVRLACLDMADARALKEIKAKSPLPLI
VDIHFNHNLAVYCAEFIDGVRINPGNIGSKENIKEVVKACKERGIPIRIGINHGSIEKQFSDKFGYGVDAMLESAMYNIK
LLEDLDFFDIKISMKTSDAQKTIEAYERLRPLCDYPFHLGVTEAGTKFHSTVKSSIALGNLLLKGIGDTMRVSMTGELEE
EIRVARAILQDSGVQKSGVNIISCPTCGRIQSDLLSAIKIVEEKTKHIKEPLNISVMGCVVNALGEAKGADVAIAFGKNQ
GLVIRHGEVVAKLKESELVDRFLAEVEDEVKSRAVKE

Sequences:

>Translated_357_residues
MEYKRFKTRQIKVGNVLIGGDAPISVQSMLFTKTRDIEGSLEQINRLYFAGANIVRLACLDMADARALKEIKAKSPLPLI
VDIHFNHNLAVYCAEFIDGVRINPGNIGSKENIKEVVKACKERGIPIRIGINHGSIEKQFSDKFGYGVDAMLESAMYNIK
LLEDLDFFDIKISMKTSDAQKTIEAYERLRPLCDYPFHLGVTEAGTKFHSTVKSSIALGNLLLKGIGDTMRVSMTGELEE
EIRVARAILQDSGVQKSGVNIISCPTCGRIQSDLLSAIKIVEEKTKHIKEPLNISVMGCVVNALGEAKGADVAIAFGKNQ
GLVIRHGEVVAKLKESELVDRFLAEVEDEVKSRAVKE
>Mature_357_residues
MEYKRFKTRQIKVGNVLIGGDAPISVQSMLFTKTRDIEGSLEQINRLYFAGANIVRLACLDMADARALKEIKAKSPLPLI
VDIHFNHNLAVYCAEFIDGVRINPGNIGSKENIKEVVKACKERGIPIRIGINHGSIEKQFSDKFGYGVDAMLESAMYNIK
LLEDLDFFDIKISMKTSDAQKTIEAYERLRPLCDYPFHLGVTEAGTKFHSTVKSSIALGNLLLKGIGDTMRVSMTGELEE
EIRVARAILQDSGVQKSGVNIISCPTCGRIQSDLLSAIKIVEEKTKHIKEPLNISVMGCVVNALGEAKGADVAIAFGKNQ
GLVIRHGEVVAKLKESELVDRFLAEVEDEVKSRAVKE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=345, Percent_Identity=41.4492753623188, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 39352; Mature: 39352

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKRFKTRQIKVGNVLIGGDAPISVQSMLFTKTRDIEGSLEQINRLYFAGANIVRLACL
CCCCCCCCCEEEECEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEHHHH
DMADARALKEIKAKSPLPLIVDIHFNHNLAVYCAEFIDGVRINPGNIGSKENIKEVVKAC
HHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHH
KERGIPIRIGINHGSIEKQFSDKFGYGVDAMLESAMYNIKLLEDLDFFDIKISMKTSDAQ
HHCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCEEEECCCCEEEEEEEEECCHHH
KTIEAYERLRPLCDYPFHLGVTEAGTKFHSTVKSSIALGNLLLKGIGDTMRVSMTGELEE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
EIRVARAILQDSGVQKSGVNIISCPTCGRIQSDLLSAIKIVEEKTKHIKEPLNISVMGCV
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
VNALGEAKGADVAIAFGKNQGLVIRHGEVVAKLKESELVDRFLAEVEDEVKSRAVKE
HHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEYKRFKTRQIKVGNVLIGGDAPISVQSMLFTKTRDIEGSLEQINRLYFAGANIVRLACL
CCCCCCCCCEEEECEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEHHHH
DMADARALKEIKAKSPLPLIVDIHFNHNLAVYCAEFIDGVRINPGNIGSKENIKEVVKAC
HHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHH
KERGIPIRIGINHGSIEKQFSDKFGYGVDAMLESAMYNIKLLEDLDFFDIKISMKTSDAQ
HHCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCEEEECCCCEEEEEEEEECCHHH
KTIEAYERLRPLCDYPFHLGVTEAGTKFHSTVKSSIALGNLLLKGIGDTMRVSMTGELEE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
EIRVARAILQDSGVQKSGVNIISCPTCGRIQSDLLSAIKIVEEKTKHIKEPLNISVMGCV
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
VNALGEAKGADVAIAFGKNQGLVIRHGEVVAKLKESELVDRFLAEVEDEVKSRAVKE
HHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA