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The map label for this gene is mltD [H]

Identifier: 57236958

GI number: 57236958

Start: 683873

End: 684991

Strand: Direct

Name: mltD [H]

Synonym: CJE0748

Alternate gene names: 57236958

Gene position: 683873-684991 (Clockwise)

Preceding gene: 57236957

Following gene: 57236959

Centisome position: 38.47

GC content: 28.42

Gene sequence:

>1119_bases
ATGAAAAAAATTTTATTGTGTTTTTTAATATGTTTTAATTTTCTTTTTGCACAAATTAATACTCCTGAATTTTATGAAAG
ACAAATGAATGTTTTAAGAAATTTAGATATTAATCCAAGTTTTATCAGTGATTTGATTTTTGTGCAAACTCAACAAGATA
TAAAATCAAAACATGCTCAAACTTTAATAGATAGTATGCAAAATTTTTCAAAAGTTACTCCTATGATAAGAAAAATTTTA
GCCCAGCAGGAAGTTCCTGATGAGATTTTGTATCTAGCTATGGTTGAATCAGGCTTGAAAACTCATAGCGTTTCTAATGC
CAAGGCTGTAGGGGTTTGGCAATTTATGCAACCAACAGCTAGAAATTTGGGTTTAAGAATCGATGCTTATGTTGATGAAA
GGCGTGATCCAGTCAAGTCAACTTATGCCGCTACAAATTATTTAAAAGAGTTAAAAGAAGAATTTGGCAAATGGTATTTA
GCACTTTTAGCTTATAATTGCGGAAATGGCAAGCTAAGACAAGCTATCAAGCAAGCAGGAAGTGATGATTTAAGTGTTTT
ATTGAACCCTGATAAGAAATATCTTTCTTTAGAAACTAGAAATTTTATAAGAAAGATTTTAACGCTTGCTTTTTTAGCTA
ATGATAGGGATTTTTTACTAGATAAAGATGCGTCTTTAATGAATTATGCTTTAAGCAATGAATTTGCAAAAGTTGATGTG
CCTTCTTCTGCATCTTTAAAAGAAATAGCTAAAAATTTAAATATGGATCTTGCAACTTTTAAGAAATATAATCCACAATT
TAAACATAATTTTACGCCTCCTGGTAAAGGGTATTATATGTATATACCACTTAATAAAGTAGCATTTTTTGATAAAAATT
TTAAAGCAGAAAAACTTGCGAAGGTTGATACAACTATACCTATGACAAGAACTTATACCGTTAAGTCTGGTGACTCTTTA
TATAAAATAGCAAAAAATTATAATATAAGTGTTGATGAAATTCGAGAATTCAATAAAATAGCAAAAAATCATCTTAGTAT
TAATCAAAAATTAATTATACCAATCAAGGAGAATAAAAATGCAAATAAAAACAATTACACTAAAGTTGTCAGCCGTTAG

Upstream 100 bases:

>100_bases
TTGTAGCAAACAAAATGTGTGAAGTATTGAATTTATCAAGAAAAGAACTTCTAGAAATTTGTTTTAACAATTCTGAAAAA
TTATTTTTTAAAGGTTATTA

Downstream 100 bases:

>100_bases
TCTAGGGGCTTTATTTTTTAGTGGTTGTTTAGGCACTAGTTTTTTTTCTAGCTTGGATAATGCTCAAGTGTATTATCCTT
CAAATGATTTTAAAAGCAGT

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKKILLCFLICFNFLFAQINTPEFYERQMNVLRNLDINPSFISDLIFVQTQQDIKSKHAQTLIDSMQNFSKVTPMIRKIL
AQQEVPDEILYLAMVESGLKTHSVSNAKAVGVWQFMQPTARNLGLRIDAYVDERRDPVKSTYAATNYLKELKEEFGKWYL
ALLAYNCGNGKLRQAIKQAGSDDLSVLLNPDKKYLSLETRNFIRKILTLAFLANDRDFLLDKDASLMNYALSNEFAKVDV
PSSASLKEIAKNLNMDLATFKKYNPQFKHNFTPPGKGYYMYIPLNKVAFFDKNFKAEKLAKVDTTIPMTRTYTVKSGDSL
YKIAKNYNISVDEIREFNKIAKNHLSINQKLIIPIKENKNANKNNYTKVVSR

Sequences:

>Translated_372_residues
MKKILLCFLICFNFLFAQINTPEFYERQMNVLRNLDINPSFISDLIFVQTQQDIKSKHAQTLIDSMQNFSKVTPMIRKIL
AQQEVPDEILYLAMVESGLKTHSVSNAKAVGVWQFMQPTARNLGLRIDAYVDERRDPVKSTYAATNYLKELKEEFGKWYL
ALLAYNCGNGKLRQAIKQAGSDDLSVLLNPDKKYLSLETRNFIRKILTLAFLANDRDFLLDKDASLMNYALSNEFAKVDV
PSSASLKEIAKNLNMDLATFKKYNPQFKHNFTPPGKGYYMYIPLNKVAFFDKNFKAEKLAKVDTTIPMTRTYTVKSGDSL
YKIAKNYNISVDEIREFNKIAKNHLSINQKLIIPIKENKNANKNNYTKVVSR
>Mature_372_residues
MKKILLCFLICFNFLFAQINTPEFYERQMNVLRNLDINPSFISDLIFVQTQQDIKSKHAQTLIDSMQNFSKVTPMIRKIL
AQQEVPDEILYLAMVESGLKTHSVSNAKAVGVWQFMQPTARNLGLRIDAYVDERRDPVKSTYAATNYLKELKEEFGKWYL
ALLAYNCGNGKLRQAIKQAGSDDLSVLLNPDKKYLSLETRNFIRKILTLAFLANDRDFLLDKDASLMNYALSNEFAKVDV
PSSASLKEIAKNLNMDLATFKKYNPQFKHNFTPPGKGYYMYIPLNKVAFFDKNFKAEKLAKVDTTIPMTRTYTVKSGDSL
YKIAKNYNISVDEIREFNKIAKNHLSINQKLIIPIKENKNANKNNYTKVVSR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=303, Percent_Identity=28.7128712871287, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42803; Mature: 42803

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLCFLICFNFLFAQINTPEFYERQMNVLRNLDINPSFISDLIFVQTQQDIKSKHAQ
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
TLIDSMQNFSKVTPMIRKILAQQEVPDEILYLAMVESGLKTHSVSNAKAVGVWQFMQPTA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCEEHHHHHCCHH
RNLGLRIDAYVDERRDPVKSTYAATNYLKELKEEFGKWYLALLAYNCGNGKLRQAIKQAG
HHCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCC
SDDLSVLLNPDKKYLSLETRNFIRKILTLAFLANDRDFLLDKDASLMNYALSNEFAKVDV
CCCEEEEECCCHHHEEHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEC
PSSASLKEIAKNLNMDLATFKKYNPQFKHNFTPPGKGYYMYIPLNKVAFFDKNFKAEKLA
CCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHH
KVDTTIPMTRTYTVKSGDSLYKIAKNYNISVDEIREFNKIAKNHLSINQKLIIPIKENKN
HHCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
ANKNNYTKVVSR
CCCCCHHHHCCC
>Mature Secondary Structure
MKKILLCFLICFNFLFAQINTPEFYERQMNVLRNLDINPSFISDLIFVQTQQDIKSKHAQ
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
TLIDSMQNFSKVTPMIRKILAQQEVPDEILYLAMVESGLKTHSVSNAKAVGVWQFMQPTA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCEEHHHHHCCHH
RNLGLRIDAYVDERRDPVKSTYAATNYLKELKEEFGKWYLALLAYNCGNGKLRQAIKQAG
HHCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCC
SDDLSVLLNPDKKYLSLETRNFIRKILTLAFLANDRDFLLDKDASLMNYALSNEFAKVDV
CCCEEEEECCCHHHEEHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEC
PSSASLKEIAKNLNMDLATFKKYNPQFKHNFTPPGKGYYMYIPLNKVAFFDKNFKAEKLA
CCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHH
KVDTTIPMTRTYTVKSGDSLYKIAKNYNISVDEIREFNKIAKNHLSINQKLIIPIKENKN
HHCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
ANKNNYTKVVSR
CCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]