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The map label for this gene is gyrB [H]

Identifier: 57236917

GI number: 57236917

Start: 2579

End: 4888

Strand: Direct

Name: gyrB [H]

Synonym: CJE0003

Alternate gene names: 57236917

Gene position: 2579-4888 (Clockwise)

Preceding gene: 57236916

Following gene: 57236920

Centisome position: 0.15

GC content: 33.03

Gene sequence:

>2310_bases
ATGCAAGAAAATTACGGTGCGAGTAATATTAAAGTCCTAAAAGGCCTAGAAGCTGTTAGAAAACGCCCAGGTATGTATAT
AGGAGATACAAACATAGGCGGACTTCATCATATGATTTATGAAGTTGTGGATAATTCTATCGATGAAGCTATGGCAGGAC
ATTGTGATACTATAGATGTAGAAATCACTACTGAAGGAAGCTGTATAGTTAGTGATAATGGTCGTGGTATTCCTGTTGAT
ATGCACCCAACTGAAAATATGCCAACTTTAACTGTTGTTTTAACTGTCCTACATGCAGGGGGAAAATTCGATAAAGATAC
TTACAAAGTTTCAGGCGGTTTGCACGGTGTTGGGGTTTCAGTTGTAAATGCACTCTCTAAAAAACTTGTAGCTACAGTTG
AAAGAAATGGAGAAATTTATCGTCAAGAATTTTCAGAAGGTAAAGTTATCAGTGAATTTGGTGTGATAGGAAAAAGTAAA
AAAACAGGAACAACTATAGAATTTTGGCCTGATGATCAAATTTTTGAAGTGACTGAATTTGATTATGAAATTTTGGCTAA
AAGATTTCGTGAACTTGCATACTTAAATCCAAAAATCACTATAAATTTTAAAGATAACCGCGTAGGCAAACATGAAAGTT
TTCACTTTGAAGGTGGAATTTCTCAGTTTGTTATAGACTTAAATAAAAAAGAAGCTTTAACTAAAGCAATTTTCTTTAGC
GTAGATGAAGAAGATGTGAATGTTGAAGTAGCTTTGCTTTACAATGATACTTATAGTGAAAATTTACTCTCTTTTGTAAA
TAATATCAAAACTCCAGATGGTGGAACACACGAAGCTGGTTTTAGAATGGGTTTAACTCGTGTGATAAGTAACTATATAG
AAGCAAATGCAAGTGCTAGAGAAAAGGATAATAAAATCACAGGCGAAGATGTGCGTGAAGGCTTGATCGCTGTTGTGAGT
GTAAAGGTGCCTGAACCACAATTTGAAGGACAAACCAAAGGAAAACTTGGTTCAACTTATGTGCGTCCTATAGTTTCAAA
AGCAAGTTTTGAATATTTGACTAAATATTTTGAAGAAAATCCTATCGAAGCTAAAGCTATAATGAACAAAGTCTTAATGG
CAGCTAGAGGAAGAGAAGCAGCGAAAAAAGCTAGAGAATTAACGCGTAAAAAAGAAAGTTTAAGCGTAGGAACTTTACCA
GGGAAATTAGCTGATTGTCAAAGTAAAGATCCAAGTGAAAGTGAAATTTATCTTGTGGAAGGGGATTCTGCAGGAGGTTC
TGCAAAACAAGGTAGAGAAAGATCTTTCCAAGCTATACTGCCTTTGCGTGGTAAAATTTTAAATGTTGAAAAAGCAAGAC
TAGATAAAATTTTAAAATCTGAGCAAATTCAAAATATGATTACCGCTTTTGGCTGTGGTATAGGTGAAGATTTTGATCTT
TCAAAACTTAGATATCATAAAATCATCATCATGACAGATGCGGATGTTGATGGATCACATATACAAACCTTGCTTTTAAC
TTTCTTCTTCCGTTTTATGAATGAACTTGTAGCAAATGGACATATTTATCTAGCACAACCACCTTTATATCTTTATAAAA
AAGCTAAAAAGCAAATTTATTTAAAAGATGAAAAAGCTTTGAGCGAATACCTGATAGAAACAGGAATAGAAGGTTTAAAC
TATGAAGGTATAGGAATGAATGATTTAAAAGATTATTTAAAAATCGTTGCAGCTTATCGTGCGATTTTAAAAGATCTTGA
AAAGCGTTTTAATGTGATTTCTGTGATACGCTATATGATAGAAAATTCAAATTTAGTTAAAGGAAATAATGAAGAATTAT
TTAGTGTAATCAAACAATTTTTAGAAACACAAGGACACAATATCTTAAATCATTATATCAACGAAAATGAAATTCGAGCT
TTCGTTCAAACTCAAAATGGCTTAGAAGAACTTGTGATCAATGAAGAACTTTTCACTCATCCACTATATGAAGAAGCAAA
TTATATTTTTGATAAGATTAAAGATAGAGGCTTGGAATTTGATAAAGATATTTTAGAAGTTCTTGAAGATGTTGAAACCA
ATGCTAAAAAAGGTGCTACTATACAACGCTATAAAGGTTTAGGGGAAATGAATCCTGAGCAACTTTGGGAAACCACAATG
GATCCAAGTGTAAGAAGACTTTTAAAAATCACTATTGAAGATGCACAAAGTGCAAATGATACCTTTAATCTCTTTATGGG
TGATGAGGTTGAACCAAGACGCGATTATATCCAAGCACACGCTAAAGATGTAAAGCATTTGGATGTGTAA

Upstream 100 bases:

>100_bases
AATGAACCTAATTCAGCATTTATAGTCAAATCCCAAGGACTATCAATGATTATTATGCCTATGATTTTGTAATAAAACAA
GTAAAAGATAAAGGAAAAAT

Downstream 100 bases:

>100_bases
ATTTATCATTGAAGAAATGATTTCTTCAATGAGTTTTGTTTTGTAAGAGTATAGCTAGAGGAATTCTTCTTCTTGTATCG
TATTTTTCTCCATAATATTT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MQENYGASNIKVLKGLEAVRKRPGMYIGDTNIGGLHHMIYEVVDNSIDEAMAGHCDTIDVEITTEGSCIVSDNGRGIPVD
MHPTENMPTLTVVLTVLHAGGKFDKDTYKVSGGLHGVGVSVVNALSKKLVATVERNGEIYRQEFSEGKVISEFGVIGKSK
KTGTTIEFWPDDQIFEVTEFDYEILAKRFRELAYLNPKITINFKDNRVGKHESFHFEGGISQFVIDLNKKEALTKAIFFS
VDEEDVNVEVALLYNDTYSENLLSFVNNIKTPDGGTHEAGFRMGLTRVISNYIEANASAREKDNKITGEDVREGLIAVVS
VKVPEPQFEGQTKGKLGSTYVRPIVSKASFEYLTKYFEENPIEAKAIMNKVLMAARGREAAKKARELTRKKESLSVGTLP
GKLADCQSKDPSESEIYLVEGDSAGGSAKQGRERSFQAILPLRGKILNVEKARLDKILKSEQIQNMITAFGCGIGEDFDL
SKLRYHKIIIMTDADVDGSHIQTLLLTFFFRFMNELVANGHIYLAQPPLYLYKKAKKQIYLKDEKALSEYLIETGIEGLN
YEGIGMNDLKDYLKIVAAYRAILKDLEKRFNVISVIRYMIENSNLVKGNNEELFSVIKQFLETQGHNILNHYINENEIRA
FVQTQNGLEELVINEELFTHPLYEEANYIFDKIKDRGLEFDKDILEVLEDVETNAKKGATIQRYKGLGEMNPEQLWETTM
DPSVRRLLKITIEDAQSANDTFNLFMGDEVEPRRDYIQAHAKDVKHLDV

Sequences:

>Translated_769_residues
MQENYGASNIKVLKGLEAVRKRPGMYIGDTNIGGLHHMIYEVVDNSIDEAMAGHCDTIDVEITTEGSCIVSDNGRGIPVD
MHPTENMPTLTVVLTVLHAGGKFDKDTYKVSGGLHGVGVSVVNALSKKLVATVERNGEIYRQEFSEGKVISEFGVIGKSK
KTGTTIEFWPDDQIFEVTEFDYEILAKRFRELAYLNPKITINFKDNRVGKHESFHFEGGISQFVIDLNKKEALTKAIFFS
VDEEDVNVEVALLYNDTYSENLLSFVNNIKTPDGGTHEAGFRMGLTRVISNYIEANASAREKDNKITGEDVREGLIAVVS
VKVPEPQFEGQTKGKLGSTYVRPIVSKASFEYLTKYFEENPIEAKAIMNKVLMAARGREAAKKARELTRKKESLSVGTLP
GKLADCQSKDPSESEIYLVEGDSAGGSAKQGRERSFQAILPLRGKILNVEKARLDKILKSEQIQNMITAFGCGIGEDFDL
SKLRYHKIIIMTDADVDGSHIQTLLLTFFFRFMNELVANGHIYLAQPPLYLYKKAKKQIYLKDEKALSEYLIETGIEGLN
YEGIGMNDLKDYLKIVAAYRAILKDLEKRFNVISVIRYMIENSNLVKGNNEELFSVIKQFLETQGHNILNHYINENEIRA
FVQTQNGLEELVINEELFTHPLYEEANYIFDKIKDRGLEFDKDILEVLEDVETNAKKGATIQRYKGLGEMNPEQLWETTM
DPSVRRLLKITIEDAQSANDTFNLFMGDEVEPRRDYIQAHAKDVKHLDV
>Mature_769_residues
MQENYGASNIKVLKGLEAVRKRPGMYIGDTNIGGLHHMIYEVVDNSIDEAMAGHCDTIDVEITTEGSCIVSDNGRGIPVD
MHPTENMPTLTVVLTVLHAGGKFDKDTYKVSGGLHGVGVSVVNALSKKLVATVERNGEIYRQEFSEGKVISEFGVIGKSK
KTGTTIEFWPDDQIFEVTEFDYEILAKRFRELAYLNPKITINFKDNRVGKHESFHFEGGISQFVIDLNKKEALTKAIFFS
VDEEDVNVEVALLYNDTYSENLLSFVNNIKTPDGGTHEAGFRMGLTRVISNYIEANASAREKDNKITGEDVREGLIAVVS
VKVPEPQFEGQTKGKLGSTYVRPIVSKASFEYLTKYFEENPIEAKAIMNKVLMAARGREAAKKARELTRKKESLSVGTLP
GKLADCQSKDPSESEIYLVEGDSAGGSAKQGRERSFQAILPLRGKILNVEKARLDKILKSEQIQNMITAFGCGIGEDFDL
SKLRYHKIIIMTDADVDGSHIQTLLLTFFFRFMNELVANGHIYLAQPPLYLYKKAKKQIYLKDEKALSEYLIETGIEGLN
YEGIGMNDLKDYLKIVAAYRAILKDLEKRFNVISVIRYMIENSNLVKGNNEELFSVIKQFLETQGHNILNHYINENEIRA
FVQTQNGLEELVINEELFTHPLYEEANYIFDKIKDRGLEFDKDILEVLEDVETNAKKGATIQRYKGLGEMNPEQLWETTM
DPSVRRLLKITIEDAQSANDTFNLFMGDEVEPRRDYIQAHAKDVKHLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=575, Percent_Identity=26.4347826086956, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=564, Percent_Identity=28.0141843971631, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI48994957, Length=813, Percent_Identity=48.7084870848708, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=547, Percent_Identity=40.5850091407678, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535065, Length=553, Percent_Identity=24.5931283905967, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=503, Percent_Identity=26.2425447316103, Blast_Score=122, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6324241, Length=547, Percent_Identity=28.8848263254113, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17136538, Length=511, Percent_Identity=28.3757338551859, Blast_Score=138, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 86687; Mature: 86687

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENYGASNIKVLKGLEAVRKRPGMYIGDTNIGGLHHMIYEVVDNSIDEAMAGHCDTIDV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
EITTEGSCIVSDNGRGIPVDMHPTENMPTLTVVLTVLHAGGKFDKDTYKVSGGLHGVGVS
EEEECCCEEECCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSKKLVATVERNGEIYRQEFSEGKVISEFGVIGKSKKTGTTIEFWPDDQIFEVTEF
HHHHHHHHHHHHHHCCCHHHHHHHCCCCEEHHHCCCCCCCCCCCEEEECCCCCEEEHHHC
DYEILAKRFRELAYLNPKITINFKDNRVGKHESFHFEGGISQFVIDLNKKEALTKAIFFS
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCHHHEEEECCHHHHHHHHHHEE
VDEEDVNVEVALLYNDTYSENLLSFVNNIKTPDGGTHEAGFRMGLTRVISNYIEANASAR
ECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
EKDNKITGEDVREGLIAVVSVKVPEPQFEGQTKGKLGSTYVRPIVSKASFEYLTKYFEEN
HCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PIEAKAIMNKVLMAARGREAAKKARELTRKKESLSVGTLPGKLADCQSKDPSESEIYLVE
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEE
GDSAGGSAKQGRERSFQAILPLRGKILNVEKARLDKILKSEQIQNMITAFGCGIGEDFDL
CCCCCCCCCCCHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SKLRYHKIIIMTDADVDGSHIQTLLLTFFFRFMNELVANGHIYLAQPPLYLYKKAKKQIY
CCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHEE
LKDEKALSEYLIETGIEGLNYEGIGMNDLKDYLKIVAAYRAILKDLEKRFNVISVIRYMI
ECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENSNLVKGNNEELFSVIKQFLETQGHNILNHYINENEIRAFVQTQNGLEELVINEELFTH
CCCCEECCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHCHHHHHC
PLYEEANYIFDKIKDRGLEFDKDILEVLEDVETNAKKGATIQRYKGLGEMNPEQLWETTM
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHCC
DPSVRRLLKITIEDAQSANDTFNLFMGDEVEPRRDYIQAHAKDVKHLDV
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQENYGASNIKVLKGLEAVRKRPGMYIGDTNIGGLHHMIYEVVDNSIDEAMAGHCDTIDV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
EITTEGSCIVSDNGRGIPVDMHPTENMPTLTVVLTVLHAGGKFDKDTYKVSGGLHGVGVS
EEEECCCEEECCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSKKLVATVERNGEIYRQEFSEGKVISEFGVIGKSKKTGTTIEFWPDDQIFEVTEF
HHHHHHHHHHHHHHCCCHHHHHHHCCCCEEHHHCCCCCCCCCCCEEEECCCCCEEEHHHC
DYEILAKRFRELAYLNPKITINFKDNRVGKHESFHFEGGISQFVIDLNKKEALTKAIFFS
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCHHHEEEECCHHHHHHHHHHEE
VDEEDVNVEVALLYNDTYSENLLSFVNNIKTPDGGTHEAGFRMGLTRVISNYIEANASAR
ECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
EKDNKITGEDVREGLIAVVSVKVPEPQFEGQTKGKLGSTYVRPIVSKASFEYLTKYFEEN
HCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PIEAKAIMNKVLMAARGREAAKKARELTRKKESLSVGTLPGKLADCQSKDPSESEIYLVE
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEE
GDSAGGSAKQGRERSFQAILPLRGKILNVEKARLDKILKSEQIQNMITAFGCGIGEDFDL
CCCCCCCCCCCHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SKLRYHKIIIMTDADVDGSHIQTLLLTFFFRFMNELVANGHIYLAQPPLYLYKKAKKQIY
CCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHEE
LKDEKALSEYLIETGIEGLNYEGIGMNDLKDYLKIVAAYRAILKDLEKRFNVISVIRYMI
ECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENSNLVKGNNEELFSVIKQFLETQGHNILNHYINENEIRAFVQTQNGLEELVINEELFTH
CCCCEECCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHCHHHHHC
PLYEEANYIFDKIKDRGLEFDKDILEVLEDVETNAKKGATIQRYKGLGEMNPEQLWETTM
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHCC
DPSVRRLLKITIEDAQSANDTFNLFMGDEVEPRRDYIQAHAKDVKHLDV
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]