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The map label for this gene is dnaN [H]

Identifier: 57236916

GI number: 57236916

Start: 1483

End: 2550

Strand: Direct

Name: dnaN [H]

Synonym: CJE0002

Alternate gene names: 57236916

Gene position: 1483-2550 (Clockwise)

Preceding gene: 57236915

Following gene: 57236917

Centisome position: 0.08

GC content: 26.22

Gene sequence:

>1068_bases
ATGAAGTTAAGTATCAATAAAAATACTTTAGAATCTGCAGTGATTTTATGTAATGCTTATGTAGAAAAAAAAGACTCAAG
CACCATTACTTCTCATCTTTTTTTTCATGCTGATGAAGATAAACTTCTTATTAGAGCTAGTGATTATGAAATAGGTATCA
ACTATAAAATAAAAAAAATCCGCGTAGAATCAAGTGGTTTTGCTACTGCAAACGCAAAAAGTATTGCAGATGTTATTAAA
AGCTTAAACAATGAAGAAGTTGTTTTAGAAACCATTGATAATTTTTTATTTATAAGACAAAAGAGTACAAAATACAAACT
TCCTATGTTTAATCACGAAGATTTTCCAAATTTTCCAAATACAGAAGGAAAAAACCAATTTGACATTGATTCAAGTGATT
TAAGCCGTTCTCTTAAAAAGATATTACCAAGTATTGATACAAACAACCCAAAATACTCCTTAAATGGTGCATTTTTAGAT
ATAAAAACAGATAAAATTAACTTCGTAGGAACTGATACAAAACGCCTTGCAATCTATACTTTAGAAAAAGCAAATAATCA
AGAATTTAGTTTTAGTATCCCTAAAAAAGCTATTATGGAAATGCAAAAACTTTTCTATGAAAAAATAGAAATTTTTTATG
ATCAAAATATGCTTATTGCTAAAAATGAAAACTTTGAATTCTTTACAAAACTTATTAACGATAAATTTCCAGATTATGAA
AAAGTTATACCAAAAACTTTCAAACAAGAACTCAGCTTTTCAACTGAAGATTTTATAGATAGTCTTAAAAAAATCAGCGT
TGTAACTGAAAAAATGAAACTTCATTTTAACAAAGATAAAATCATCTTTGAAGGTATAAGTTTAGACAATATGGAAGCAA
AAACAGAGCTTGAAATTCAAACAGGAGTAAGTGAAGAATTTAATCTTACTATAAAAATCAAATATTTACTTGATTTCTTA
ACTTCTATAGAAGAAGAAAAATTCACTTTAAGTGTAAATGAACCTAATTCAGCATTTATAGTCAAATCCCAAGGACTATC
AATGATTATTATGCCTATGATTTTGTAA

Upstream 100 bases:

>100_bases
ATAAAATACAAATTTGATTATTTTGCTTTGAAGAATTTCACAATTTCAACAAGCTTATTATTACAACGAATTTAAAATTA
AAATAAACCAAGGAGAAAAA

Downstream 100 bases:

>100_bases
TAAAACAAGTAAAAGATAAAGGAAAAATATGCAAGAAAATTACGGTGCGAGTAATATTAAAGTCCTAAAAGGCCTAGAAG
CTGTTAGAAAACGCCCAGGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKIRVESSGFATANAKSIADVIK
SLNNEEVVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPNTEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLD
IKTDKINFVGTDTKRLAIYTLEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKDKIIFEGISLDNMEAKTELEIQTGVSEEFNLTIKIKYLLDFL
TSIEEEKFTLSVNEPNSAFIVKSQGLSMIIMPMIL

Sequences:

>Translated_355_residues
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKIRVESSGFATANAKSIADVIK
SLNNEEVVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPNTEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLD
IKTDKINFVGTDTKRLAIYTLEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKDKIIFEGISLDNMEAKTELEIQTGVSEEFNLTIKIKYLLDFL
TSIEEEKFTLSVNEPNSAFIVKSQGLSMIIMPMIL
>Mature_355_residues
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKIRVESSGFATANAKSIADVIK
SLNNEEVVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPNTEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLD
IKTDKINFVGTDTKRLAIYTLEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKDKIIFEGISLDNMEAKTELEIQTGVSEEFNLTIKIKYLLDFL
TSIEEEKFTLSVNEPNSAFIVKSQGLSMIIMPMIL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=18.8010899182561, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40979; Mature: 40979

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKI
CEEECCCCHHHHHHEEEEHHHCCCCCCCEEEEEEEEECCCEEEEEECCEEEECEEEEEEE
RVESSGFATANAKSIADVIKSLNNEEVVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPN
EEECCCCCCCCHHHHHHHHHHCCCCEEEHEECCCEEEEEECCCEEECCCCCCCCCCCCCC
TEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLDIKTDKINFVGTDTKRLAIYT
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECEEEEEEEECEEEEEECCCCEEEEEE
LEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
EECCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCEEEEECCCHHHHHHHHCCCCCCHH
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKDKIIFEGISLDNMEAKTELEIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCEEEEEE
TGVSEEFNLTIKIKYLLDFLTSIEEEKFTLSVNEPNSAFIVKSQGLSMIIMPMIL
ECCCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCC
>Mature Secondary Structure
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKI
CEEECCCCHHHHHHEEEEHHHCCCCCCCEEEEEEEEECCCEEEEEECCEEEECEEEEEEE
RVESSGFATANAKSIADVIKSLNNEEVVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPN
EEECCCCCCCCHHHHHHHHHHCCCCEEEHEECCCEEEEEECCCEEECCCCCCCCCCCCCC
TEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLDIKTDKINFVGTDTKRLAIYT
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECEEEEEEEECEEEEEECCCCEEEEEE
LEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
EECCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCEEEEECCCHHHHHHHHCCCCCCHH
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKDKIIFEGISLDNMEAKTELEIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCEEEEEE
TGVSEEFNLTIKIKYLLDFLTSIEEEKFTLSVNEPNSAFIVKSQGLSMIIMPMIL
ECCCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]