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The map label for this gene is ruvC [H]

Identifier: 57236914

GI number: 57236914

Start: 1777254

End: 1777736

Strand: Reverse

Name: ruvC [H]

Synonym: CJE1897

Alternate gene names: 57236914

Gene position: 1777736-1777254 (Counterclockwise)

Preceding gene: NA

Following gene: 57236913

Centisome position: 99.99

GC content: 34.99

Gene sequence:

>483_bases
ATGAATTTGAAAATTTTAGGTATAGATCCAGGTTCAAGAAATTGCGGTTATGCTATCATAGAAGCCAACAAAGGAAAAAA
CATTCTCATAGAAGCAGGACTTATAAAGATAAAGCCAAGTACCTTACAGTATCAAATCACAGAGCTTTGTGAGGGTTTGG
ACTTGATTTTTAAAAATCATTCTTTTGATGAGGTGGCTATAGAAGATATCTTTTTTGCTTACAATCCAAAAACCGTTTTA
AAACTCGCACAATTTCGCGGAGCCCTATCTTTAAAAATTCTTCAAATTCATGGAGATTTTGCAGAATACACTCCCTTACA
AGTAAAAAAAGCCGTTACAGGAAAAGCCAAAGCAACTAAAGAACAAGTTGCTTTTATGGTAAAAAGGCTTTTAGGACTTA
GTAAAGATATCAAGCCTTTAGACATCACAGATGCTATCGCAGTGGCTTTAACTCATGCGGCAAATTTAAGAGTTAGGGTG
TGA

Upstream 100 bases:

>100_bases
TTCATAATCTCTCTTTAAATTTTGCATAATGTATAAATTTTATCTTAAATTTTATAAAAAAAGGTTAAAATCCGCTTTCG
TATTTTATTTTTCAAAAGGT

Downstream 100 bases:

>100_bases
TTTTGGTAATTTTTATTACACAAAAGCCCAAAGCTTCATAAACTCCCAAAATACCTTTAAATTTTTTCTATAAATTTTTA
AAAAATTCATAAAAAATTTC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MNLKILGIDPGSRNCGYAIIEANKGKNILIEAGLIKIKPSTLQYQITELCEGLDLIFKNHSFDEVAIEDIFFAYNPKTVL
KLAQFRGALSLKILQIHGDFAEYTPLQVKKAVTGKAKATKEQVAFMVKRLLGLSKDIKPLDITDAIAVALTHAANLRVRV

Sequences:

>Translated_160_residues
MNLKILGIDPGSRNCGYAIIEANKGKNILIEAGLIKIKPSTLQYQITELCEGLDLIFKNHSFDEVAIEDIFFAYNPKTVL
KLAQFRGALSLKILQIHGDFAEYTPLQVKKAVTGKAKATKEQVAFMVKRLLGLSKDIKPLDITDAIAVALTHAANLRVRV
>Mature_160_residues
MNLKILGIDPGSRNCGYAIIEANKGKNILIEAGLIKIKPSTLQYQITELCEGLDLIFKNHSFDEVAIEDIFFAYNPKTVL
KLAQFRGALSLKILQIHGDFAEYTPLQVKKAVTGKAKATKEQVAFMVKRLLGLSKDIKPLDITDAIAVALTHAANLRVRV

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=35.6060606060606, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17650; Mature: 17650

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKILGIDPGSRNCGYAIIEANKGKNILIEAGLIKIKPSTLQYQITELCEGLDLIFKNH
CCEEEEEECCCCCCCCEEEEECCCCCEEEEEECEEEECCCHHEEEHHHHHCCHHEECCCC
SFDEVAIEDIFFAYNPKTVLKLAQFRGALSLKILQIHGDFAEYTPLQVKKAVTGKAKATK
CCCCEEHHHEEEEECCHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCCHHHH
EQVAFMVKRLLGLSKDIKPLDITDAIAVALTHAANLRVRV
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEC
>Mature Secondary Structure
MNLKILGIDPGSRNCGYAIIEANKGKNILIEAGLIKIKPSTLQYQITELCEGLDLIFKNH
CCEEEEEECCCCCCCCEEEEECCCCCEEEEEECEEEECCCHHEEEHHHHHCCHHEECCCC
SFDEVAIEDIFFAYNPKTVLKLAQFRGALSLKILQIHGDFAEYTPLQVKKAVTGKAKATK
CCCCEEHHHEEEEECCHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCCHHHH
EQVAFMVKRLLGLSKDIKPLDITDAIAVALTHAANLRVRV
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA