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The map label for this gene is grpE

Identifier: 21228608

GI number: 21228608

Start: 2989196

End: 2989798

Strand: Reverse

Name: grpE

Synonym: MM_2506

Alternate gene names: 21228608

Gene position: 2989798-2989196 (Counterclockwise)

Preceding gene: 21228610

Following gene: 21228607

Centisome position: 72.99

GC content: 45.94

Gene sequence:

>603_bases
ATGGATTCTAAAGAAAGAAACCAGAAAGAGGCTGAACGTTCCGAAGCCCGAAATTCCGAATCTCCGGCTGAAAAAGCCGG
TGAGACAAAAGTAAGTCCTGAAAATGAACCTTCAAGTCCCGAAGCTGAAAAAAACCCGGAAGAGGCATGCAGGGAAGAAA
ACGAAATTCTCAAAGACCAGCTTTTCCGGCTTGCAGCGGATTTTGATAACTTCAGAAAACGGACCGCCCGTCAGATGGAA
GAAAACCGGAAATCTGTGCTTGAACAGGTGCTCCTTGACTTTGTCGAAGTAACGGATAACTTTGACCGCGCCATTAAATC
TGCAAGGACTGCAGAAGACATGGGCCCAATTGTAAGTGGAATTGAACAGCTTTCGAAGCAGTTTTTCTCTATCCTCGAAA
AGTACGGGCTTGAGAGAGTAAAATGCGAAAAAGCAGGCGAGTTTGACCCTCACAGACACGAAGCTATCCATCACATAGAA
ACCTCCGAAGTTCCGGATAACACTATTGTTGAGATTTATAAAGAGGGATATGCTTTGAACGAGAAGGTCGTCAGGCCAGC
CCTGGTTTCAGTAGCAAGGAGCCCTGAAGAGGCAGAGAAGTAA

Upstream 100 bases:

>100_bases
TATTTATAGAATTACAAACAATCACAGATCAGGACGAGCGAAACAGTTAGAATAATTTTATGGGGATTGATAAATGAAAA
AATCCAGGAAAAAGGAAAAC

Downstream 100 bases:

>100_bases
ATCCTGTTACAATATAAGACTTTATTTAATAACCTGAAACAGGGATTTATCAAGAATCAATCATTAATCTTCCATGCCAG
ATAATTTGGCAAACTCAAAC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MDSKERNQKEAERSEARNSESPAEKAGETKVSPENEPSSPEAEKNPEEACREENEILKDQLFRLAADFDNFRKRTARQME
ENRKSVLEQVLLDFVEVTDNFDRAIKSARTAEDMGPIVSGIEQLSKQFFSILEKYGLERVKCEKAGEFDPHRHEAIHHIE
TSEVPDNTIVEIYKEGYALNEKVVRPALVSVARSPEEAEK

Sequences:

>Translated_200_residues
MDSKERNQKEAERSEARNSESPAEKAGETKVSPENEPSSPEAEKNPEEACREENEILKDQLFRLAADFDNFRKRTARQME
ENRKSVLEQVLLDFVEVTDNFDRAIKSARTAEDMGPIVSGIEQLSKQFFSILEKYGLERVKCEKAGEFDPHRHEAIHHIE
TSEVPDNTIVEIYKEGYALNEKVVRPALVSVARSPEEAEK
>Mature_200_residues
MDSKERNQKEAERSEARNSESPAEKAGETKVSPENEPSSPEAEKNPEEACREENEILKDQLFRLAADFDNFRKRTARQME
ENRKSVLEQVLLDFVEVTDNFDRAIKSARTAEDMGPIVSGIEQLSKQFFSILEKYGLERVKCEKAGEFDPHRHEAIHHIE
TSEVPDNTIVEIYKEGYALNEKVVRPALVSVARSPEEAEK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=138, Percent_Identity=34.0579710144928, Blast_Score=92, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324806, Length=184, Percent_Identity=27.1739130434783, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653432, Length=163, Percent_Identity=31.9018404907975, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_METMA (P42367)

Other databases:

- EMBL:   X74353
- EMBL:   AE008384
- PIR:   S46379
- RefSeq:   NP_634530.1
- ProteinModelPortal:   P42367
- SMR:   P42367
- GeneID:   1480848
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2506
- NMPDR:   fig|192952.1.peg.2506
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14153
- BioCyc:   MMAZ192952:MM2506-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22865; Mature: 22865

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSKERNQKEAERSEARNSESPAEKAGETKVSPENEPSSPEAEKNPEEACREENEILKDQ
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LFRLAADFDNFRKRTARQMEENRKSVLEQVLLDFVEVTDNFDRAIKSARTAEDMGPIVSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQLSKQFFSILEKYGLERVKCEKAGEFDPHRHEAIHHIETSEVPDNTIVEIYKEGYALN
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHH
EKVVRPALVSVARSPEEAEK
HHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MDSKERNQKEAERSEARNSESPAEKAGETKVSPENEPSSPEAEKNPEEACREENEILKDQ
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LFRLAADFDNFRKRTARQMEENRKSVLEQVLLDFVEVTDNFDRAIKSARTAEDMGPIVSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQLSKQFFSILEKYGLERVKCEKAGEFDPHRHEAIHHIETSEVPDNTIVEIYKEGYALN
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHH
EKVVRPALVSVARSPEEAEK
HHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517454; 12125824