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The map label for this gene is fpoH

Identifier: 21228589

GI number: 21228589

Start: 2965623

End: 2966675

Strand: Reverse

Name: fpoH

Synonym: MM_2487

Alternate gene names: 21228589

Gene position: 2966675-2965623 (Counterclockwise)

Preceding gene: 21228590

Following gene: 21228588

Centisome position: 72.42

GC content: 47.48

Gene sequence:

>1053_bases
GTGACTTTTATGGCTATAGAAATTCCAGAATTCATAGTTCCCTTTGTCCCCTGGATCCGCGGTACTGTAGGCCTGGTTCT
CGTAGGAGCCATCTTCCTTGGCGGAATGGCTGCAGTCTGGATTGAGAGAAAACTCTCAGCTGACATCCAGTTAAGGTACG
GGCCTTCAAGGGTGGGAAAGTTCGGGCTCTTACAGCTTGTAGCCGATGCCATCAAACTCTTTACAAAAGAAGATGTCAGG
CCTGGAAATGCCGACCGTTTTCTTTACGACAATGCCCCGGTTTTCATGCTGACTTCCCTTTTTCTGATGCTTGTAGCGAT
TCCGGTTGGAGCGGTCTTTATTGATGGAAACTTATACCCTCTGGCCGTAACCGAGATGGATATAAGCATACTCTTCATAG
AAGCGGTGTCCGCAATCAATATTTTTGGAATATTTATGGCTGCGTACGGATCAAACAATAAATATTCCCTGCTTGGTGCT
TTCAGGAATTTTGCCCGCATGATAGGGTATGAAGTGCCACTAGGTATCGCTATTGTCAGCGTTGCCGTAATGACGGGCTC
CCTCAATATCATTGATATTACCAGTGCCCAGGGAAGTTTTGTCTGGAACATTTTCCTGCAGCCTATCGGATTTGTTGTTT
TCTTTATCGCACTTATGGCTGACCTTGGAAGGCTCCCTTTTGACCAGAACGAATCCGAAGAAGAACTGGTAGCAGGCTGG
GTAACGGAATACACAGGAATGCGTTTTGGGCTTGTCTTCTTTGCTGAGTATATGCACATGATCCTTGGATCATTCCTTGT
AGCACTCCTTTTCCTTGGAGGCTGGAACGTACCGGCATTTGTTGCAAATAATGCAGTGCTCGGACTTATTGCCCCTACAG
GAATTTTGCTCCTTAAGACCGTGCTTGTGCTGATGACTATTATAGGAATGAGGTGGGCTGTCCCGAGGTTCAGGATCGAC
CAGGTAGTGGATATGAGCTGGAAGAAACTCCTGCCCCTGTCCCTGTTGAACCTTGCCTGGGCAGTCGGCCTTGGACTTTA
TCTGGGGGCTTAA

Upstream 100 bases:

>100_bases
CGACTCTGCAGGCTCTCCCCCCTCTTATCATAGGCACAACGGTTGCAGACATGGTATCAATTTCGGGAAGCATGGACGGC
TGTACCAGTGAAGTGGACAG

Downstream 100 bases:

>100_bases
GTATGGTTCTCAAAAACATCAAATATGCGCTTAAAAACATCCCTAAAGAACGCGTGACCAGGCTGTGCCCGGAGGTGGAA
AGTCCGCTCTCCGAAAGGTT

Product: F420H2 dehydrogenase subunit

Products: NAD+; ubiquinol

Alternate protein names: F(420)H(2) dehydrogenase subunit H; FPO subunit H

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTFMAIEIPEFIVPFVPWIRGTVGLVLVGAIFLGGMAAVWIERKLSADIQLRYGPSRVGKFGLLQLVADAIKLFTKEDVR
PGNADRFLYDNAPVFMLTSLFLMLVAIPVGAVFIDGNLYPLAVTEMDISILFIEAVSAINIFGIFMAAYGSNNKYSLLGA
FRNFARMIGYEVPLGIAIVSVAVMTGSLNIIDITSAQGSFVWNIFLQPIGFVVFFIALMADLGRLPFDQNESEEELVAGW
VTEYTGMRFGLVFFAEYMHMILGSFLVALLFLGGWNVPAFVANNAVLGLIAPTGILLLKTVLVLMTIIGMRWAVPRFRID
QVVDMSWKKLLPLSLLNLAWAVGLGLYLGA

Sequences:

>Translated_350_residues
MTFMAIEIPEFIVPFVPWIRGTVGLVLVGAIFLGGMAAVWIERKLSADIQLRYGPSRVGKFGLLQLVADAIKLFTKEDVR
PGNADRFLYDNAPVFMLTSLFLMLVAIPVGAVFIDGNLYPLAVTEMDISILFIEAVSAINIFGIFMAAYGSNNKYSLLGA
FRNFARMIGYEVPLGIAIVSVAVMTGSLNIIDITSAQGSFVWNIFLQPIGFVVFFIALMADLGRLPFDQNESEEELVAGW
VTEYTGMRFGLVFFAEYMHMILGSFLVALLFLGGWNVPAFVANNAVLGLIAPTGILLLKTVLVLMTIIGMRWAVPRFRID
QVVDMSWKKLLPLSLLNLAWAVGLGLYLGA
>Mature_349_residues
TFMAIEIPEFIVPFVPWIRGTVGLVLVGAIFLGGMAAVWIERKLSADIQLRYGPSRVGKFGLLQLVADAIKLFTKEDVRP
GNADRFLYDNAPVFMLTSLFLMLVAIPVGAVFIDGNLYPLAVTEMDISILFIEAVSAINIFGIFMAAYGSNNKYSLLGAF
RNFARMIGYEVPLGIAIVSVAVMTGSLNIIDITSAQGSFVWNIFLQPIGFVVFFIALMADLGRLPFDQNESEEELVAGWV
TEYTGMRFGLVFFAEYMHMILGSFLVALLFLGGWNVPAFVANNAVLGLIAPTGILLLKTVLVLMTIIGMRWAVPRFRIDQ
VVDMSWKKLLPLSLLNLAWAVGLGLYLGA

Specific function: FPO shuttles electrons from F(420)H(2), via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H(2):heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples

COG id: COG1005

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 1 (chain H)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family

Homologues:

Organism=Homo sapiens, GI251831107, Length=312, Percent_Identity=34.6153846153846, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1788618, Length=297, Percent_Identity=42.0875420875421, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1789077, Length=291, Percent_Identity=25.4295532646048, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPOH_METMA (Q8PU59)

Other databases:

- EMBL:   AF228525
- EMBL:   AE008384
- RefSeq:   NP_634511.1
- GeneID:   1480829
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2487
- NMPDR:   fig|192952.1.peg.2487
- HOGENOM:   HBG727670
- OMA:   IFWICAL
- ProtClustDB:   CLSK568843
- BioCyc:   MMAZ192952:MM2487-MONOMER
- HAMAP:   MF_01350
- InterPro:   IPR023021
- InterPro:   IPR001694
- InterPro:   IPR018086
- PANTHER:   PTHR11432

Pfam domain/function: PF00146 NADHdh

EC number: 1.6.5.3

Molecular weight: Translated: 38443; Mature: 38312

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00667 COMPLEX1_ND1_1; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdd2f678)-; HASH(0x10756ac4)-; HASH(0xff8d830)-; HASH(0x10cbe264)-; HASH(0x10927054)-; HASH(0xb5a808c)-; HASH(0xc286d34)-; HASH(0x10d12618)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFMAIEIPEFIVPFVPWIRGTVGLVLVGAIFLGGMAAVWIERKLSADIQLRYGPSRVGK
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
FGLLQLVADAIKLFTKEDVRPGNADRFLYDNAPVFMLTSLFLMLVAIPVGAVFIDGNLYP
HHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCEEEECCCEEE
LAVTEMDISILFIEAVSAINIFGIFMAAYGSNNKYSLLGAFRNFARMIGYEVPLGIAIVS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
VAVMTGSLNIIDITSAQGSFVWNIFLQPIGFVVFFIALMADLGRLPFDQNESEEELVAGW
HHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
VTEYTGMRFGLVFFAEYMHMILGSFLVALLFLGGWNVPAFVANNAVLGLIAPTGILLLKT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHHHHHHHHHH
VLVLMTIIGMRWAVPRFRIDQVVDMSWKKLLPLSLLNLAWAVGLGLYLGA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TFMAIEIPEFIVPFVPWIRGTVGLVLVGAIFLGGMAAVWIERKLSADIQLRYGPSRVGK
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
FGLLQLVADAIKLFTKEDVRPGNADRFLYDNAPVFMLTSLFLMLVAIPVGAVFIDGNLYP
HHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCEEEECCCEEE
LAVTEMDISILFIEAVSAINIFGIFMAAYGSNNKYSLLGAFRNFARMIGYEVPLGIAIVS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
VAVMTGSLNIIDITSAQGSFVWNIFLQPIGFVVFFIALMADLGRLPFDQNESEEELVAGW
HHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
VTEYTGMRFGLVFFAEYMHMILGSFLVALLFLGGWNVPAFVANNAVLGLIAPTGILLLKT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHHHHHHHHHH
VLVLMTIIGMRWAVPRFRIDQVVDMSWKKLLPLSLLNLAWAVGLGLYLGA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10751389; 12125824