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The map label for this gene is fpoI [H]

Identifier: 21228588

GI number: 21228588

Start: 2965210

End: 2965743

Strand: Reverse

Name: fpoI [H]

Synonym: MM_2486

Alternate gene names: 21228588

Gene position: 2965743-2965210 (Counterclockwise)

Preceding gene: 21228589

Following gene: 21228587

Centisome position: 72.4

GC content: 51.87

Gene sequence:

>534_bases
GTGGGCTGTCCCGAGGTTCAGGATCGACCAGGTAGTGGATATGAGCTGGAAGAAACTCCTGCCCCTGTCCCTGTTGAACC
TTGCCTGGGCAGTCGGCCTTGGACTTTATCTGGGGGCTTAAGTATGGTTCTCAAAAACATCAAATATGCGCTTAAAAACA
TCCCTAAAGAACGCGTGACCAGGCTGTGCCCGGAGGTGGAAAGTCCGCTCTCCGAAAGGTTTAGAGGCCTCCAGACCCTT
GACAAAAGCAAATGTATAGGCTGCGGGATCTGTGCCAATACCTGCCCTAACAGCGCGATCAAGATCGTAAAAGCCCCGAT
TGCCCCTGGCAGTGAAAAGAAGAGGTGGTTCCCTCAAATAGACATAGGGCACTGCCTCTTCTGCGGGCTCTGTATAGACC
AGTGCCCCAAAGGTGCCCTTTCAAGCGGGAAAGAGTACTGCAAAGGCATGGTGAAATGGGCTCATAAAGACCTGCTTATG
ACCCCTGAAAAACTTGCGAGGGAAGTAGACATTCAGGAAGGTGACGAGAGATGA

Upstream 100 bases:

>100_bases
CGGCATTTGTTGCAAATAATGCAGTGCTCGGACTTATTGCCCCTACAGGAATTTTGCTCCTTAAGACCGTGCTTGTGCTG
ATGACTATTATAGGAATGAG

Downstream 100 bases:

>100_bases
TCGACCCTGGAACGGTTGGAGCAGCACTGGAAACGGCGGTCTTCGGACTCCTTGCACTTGTCACGGTCTTCTTTGCAATC
TTTGTGGTCATTGCGAAAGA

Product: F420H2 dehydrogenase subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MGCPEVQDRPGSGYELEETPAPVPVEPCLGSRPWTLSGGLSMVLKNIKYALKNIPKERVTRLCPEVESPLSERFRGLQTL
DKSKCIGCGICANTCPNSAIKIVKAPIAPGSEKKRWFPQIDIGHCLFCGLCIDQCPKGALSSGKEYCKGMVKWAHKDLLM
TPEKLAREVDIQEGDER

Sequences:

>Translated_177_residues
MGCPEVQDRPGSGYELEETPAPVPVEPCLGSRPWTLSGGLSMVLKNIKYALKNIPKERVTRLCPEVESPLSERFRGLQTL
DKSKCIGCGICANTCPNSAIKIVKAPIAPGSEKKRWFPQIDIGHCLFCGLCIDQCPKGALSSGKEYCKGMVKWAHKDLLM
TPEKLAREVDIQEGDER
>Mature_176_residues
GCPEVQDRPGSGYELEETPAPVPVEPCLGSRPWTLSGGLSMVLKNIKYALKNIPKERVTRLCPEVESPLSERFRGLQTLD
KSKCIGCGICANTCPNSAIKIVKAPIAPGSEKKRWFPQIDIGHCLFCGLCIDQCPKGALSSGKEYCKGMVKWAHKDLLMT
PEKLAREVDIQEGDER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=138, Percent_Identity=31.1594202898551, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788617, Length=117, Percent_Identity=32.4786324786325, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789075, Length=90, Percent_Identity=34.4444444444444, Blast_Score=60, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=145, Percent_Identity=31.7241379310345, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864306, Length=128, Percent_Identity=33.59375, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19459; Mature: 19328

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCPEVQDRPGSGYELEETPAPVPVEPCLGSRPWTLSGGLSMVLKNIKYALKNIPKERVT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
RLCPEVESPLSERFRGLQTLDKSKCIGCGICANTCPNSAIKIVKAPIAPGSEKKRWFPQI
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCC
DIGHCLFCGLCIDQCPKGALSSGKEYCKGMVKWAHKDLLMTPEKLAREVDIQEGDER
CHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GCPEVQDRPGSGYELEETPAPVPVEPCLGSRPWTLSGGLSMVLKNIKYALKNIPKERVT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
RLCPEVESPLSERFRGLQTLDKSKCIGCGICANTCPNSAIKIVKAPIAPGSEKKRWFPQI
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCC
DIGHCLFCGLCIDQCPKGALSSGKEYCKGMVKWAHKDLLMTPEKLAREVDIQEGDER
CHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA