Click here to switch to the map view.

The map label for this gene is leuC2 [H]

Identifier: 21228475

GI number: 21228475

Start: 2829592

End: 2830953

Strand: Reverse

Name: leuC2 [H]

Synonym: MM_2373

Alternate gene names: 21228475

Gene position: 2830953-2829592 (Counterclockwise)

Preceding gene: 21228476

Following gene: 21228474

Centisome position: 69.11

GC content: 48.02

Gene sequence:

>1362_bases
ATGAACTTCATTTCTTTTTGGGATCATCAATTAAATTTATTTATTAGAAAGCGCCAGTTATTTCCCATGACCGGAATGTC
TGGTAGTGACAATCGCCCGATGACCGTTTCGGAGAAGATCTTTTCGAAAGCCTCCGGAACTCCCGTGAAAGCCGGGGATT
TCGTACTGGCAAATATAGACCTGGCAATGACCCACGACATCACAGGTCCGCTGGCAGTCCAGGGCTTCTACGAAATCATG
AGAGATGAAGAAGACAAGAAAGTATGGGACCCGAGCAAAATTGTAATTATATTCGACCACCAGGTCCCTGCAGACTCAAT
TAATGCCGCTCAAAACCATATCATGCTCAGAAAGTTTGCAAAAGAGCAGGGCATTTTAAATTATGATGTTTATGAAGGAG
TCTGCCACCAAGTCCTTCCAGAAAAAGGGCATGTTAAGCCCGGAGACCTGATTGTTGGTTCTGACTCCCATACCTGTGCA
TACGGATCTTTAGGGGCATTTTCTACCGGCATAGGCTCTACCGATATGGCAGCGGTTTTTGCCACAGGCAAGCTCTGGTT
CCGGGTTCCCGAGACTTTCCGCTTTGAAGTGGAAGGAAAACTACCAGAGCGCGTTTATTCCAAAGACCTTATACTGCACC
TAATAGGAGACGTAGGCGTGGAAGGGGTCAGATACATGGCAGCCGAGTACGCGGGTTCTACAATCCGTTCCCTTTCCATC
CCCGAGCGCATGACCATGTCCAACATGGCAATTGAAATGGGAGGAAAAGCAGGGATAATTGAAGCCGATGAAGTTACCGA
GGCATACCTTAAAGAGCGCATTCCGGGTTTCAAGCTTGATCCGTACTGGAAATCCGATGAAGGTGCCAAATATCTGGATA
TCAGGTATTATGATGTTTCTGACCTTGAGCCCCAGGTAGCCTGCCCGCATAATGTTGACAACGTAAAGCCGGTAAGCGAA
GTTGAAGGCACAAAACTCGACCAGATCTTTATGGGATCCTGCACAAACGGCAGGTTTGAAGACATCAAGATAATGGCTGA
CATCATGGGAGATGAACCTGTTGCGAAAAATTTGCGCCTCCTTGTTGTTCCCGCTTCAAAAACTGAATATATGAAGCTGT
TAAAAGCCGGATATATTGAAAAACTCGTGAACGCGGGCGCAATCGTGGAAGCTCCCTGCTGCGGGCCATGCATGGGCGGC
TCTTTTGGTCTTCTCGGACCTGGAGAGGTCGGACTTGCCACCTCAAACCGCAATTTCAAGGGCAGAGAAGGAAGTGCAGA
GTCTTTTGTTTACCTTTCTTCCCCGGCAACTGCAGGAGCATCCGCCCTCACTGGAGAAATCACGGATCCCAGGAAGGTTT
AA

Upstream 100 bases:

>100_bases
TATTGATTAAGCCTCAGATTTTTTGAGTTTCCGGCTGATTCGTCCAGTTGCAAATTTTTAATTTAATGGTACTTTTTGAA
AAAATTATATTATATCATCT

Downstream 100 bases:

>100_bases
AGACCCTCCTGTTTACTTCTTCCTAATTTTTTCTACTTATTATTTAATGATGTATTATTTATCTTGAACAGGTGTTAAAA
ATGCCGGAGGCTGACTTATC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNFISFWDHQLNLFIRKRQLFPMTGMSGSDNRPMTVSEKIFSKASGTPVKAGDFVLANIDLAMTHDITGPLAVQGFYEIM
RDEEDKKVWDPSKIVIIFDHQVPADSINAAQNHIMLRKFAKEQGILNYDVYEGVCHQVLPEKGHVKPGDLIVGSDSHTCA
YGSLGAFSTGIGSTDMAAVFATGKLWFRVPETFRFEVEGKLPERVYSKDLILHLIGDVGVEGVRYMAAEYAGSTIRSLSI
PERMTMSNMAIEMGGKAGIIEADEVTEAYLKERIPGFKLDPYWKSDEGAKYLDIRYYDVSDLEPQVACPHNVDNVKPVSE
VEGTKLDQIFMGSCTNGRFEDIKIMADIMGDEPVAKNLRLLVVPASKTEYMKLLKAGYIEKLVNAGAIVEAPCCGPCMGG
SFGLLGPGEVGLATSNRNFKGREGSAESFVYLSSPATAGASALTGEITDPRKV

Sequences:

>Translated_453_residues
MNFISFWDHQLNLFIRKRQLFPMTGMSGSDNRPMTVSEKIFSKASGTPVKAGDFVLANIDLAMTHDITGPLAVQGFYEIM
RDEEDKKVWDPSKIVIIFDHQVPADSINAAQNHIMLRKFAKEQGILNYDVYEGVCHQVLPEKGHVKPGDLIVGSDSHTCA
YGSLGAFSTGIGSTDMAAVFATGKLWFRVPETFRFEVEGKLPERVYSKDLILHLIGDVGVEGVRYMAAEYAGSTIRSLSI
PERMTMSNMAIEMGGKAGIIEADEVTEAYLKERIPGFKLDPYWKSDEGAKYLDIRYYDVSDLEPQVACPHNVDNVKPVSE
VEGTKLDQIFMGSCTNGRFEDIKIMADIMGDEPVAKNLRLLVVPASKTEYMKLLKAGYIEKLVNAGAIVEAPCCGPCMGG
SFGLLGPGEVGLATSNRNFKGREGSAESFVYLSSPATAGASALTGEITDPRKV
>Mature_453_residues
MNFISFWDHQLNLFIRKRQLFPMTGMSGSDNRPMTVSEKIFSKASGTPVKAGDFVLANIDLAMTHDITGPLAVQGFYEIM
RDEEDKKVWDPSKIVIIFDHQVPADSINAAQNHIMLRKFAKEQGILNYDVYEGVCHQVLPEKGHVKPGDLIVGSDSHTCA
YGSLGAFSTGIGSTDMAAVFATGKLWFRVPETFRFEVEGKLPERVYSKDLILHLIGDVGVEGVRYMAAEYAGSTIRSLSI
PERMTMSNMAIEMGGKAGIIEADEVTEAYLKERIPGFKLDPYWKSDEGAKYLDIRYYDVSDLEPQVACPHNVDNVKPVSE
VEGTKLDQIFMGSCTNGRFEDIKIMADIMGDEPVAKNLRLLVVPASKTEYMKLLKAGYIEKLVNAGAIVEAPCCGPCMGG
SFGLLGPGEVGLATSNRNFKGREGSAESFVYLSSPATAGASALTGEITDPRKV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=455, Percent_Identity=27.6923076923077, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI8659555, Length=239, Percent_Identity=29.2887029288703, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786259, Length=448, Percent_Identity=33.7053571428571, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081781, Length=370, Percent_Identity=24.5945945945946, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367097, Length=449, Percent_Identity=24.9443207126949, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787531, Length=389, Percent_Identity=23.9074550128535, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=454, Percent_Identity=26.2114537444934, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32564738, Length=337, Percent_Identity=27.5964391691395, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17568399, Length=502, Percent_Identity=24.7011952191235, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25149342, Length=299, Percent_Identity=25.4180602006689, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=434, Percent_Identity=33.4101382488479, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321429, Length=477, Percent_Identity=31.6561844863732, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323335, Length=495, Percent_Identity=26.6666666666667, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=467, Percent_Identity=24.4111349036403, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28571643, Length=336, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281365315, Length=460, Percent_Identity=25.2173913043478, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17864292, Length=460, Percent_Identity=25.2173913043478, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161076999, Length=337, Percent_Identity=26.4094955489614, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24645686, Length=494, Percent_Identity=24.4939271255061, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137564, Length=497, Percent_Identity=24.3460764587525, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49599; Mature: 49599

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFISFWDHQLNLFIRKRQLFPMTGMSGSDNRPMTVSEKIFSKASGTPVKAGDFVLANID
CCCHHHHHHEEEEEEEEEEECEECCCCCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEEEE
LAMTHDITGPLAVQGFYEIMRDEEDKKVWDPSKIVIIFDHQVPADSINAAQNHIMLRKFA
EEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
KEQGILNYDVYEGVCHQVLPEKGHVKPGDLIVGSDSHTCAYGSLGAFSTGIGSTDMAAVF
HHCCCEEEHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCHHCCCCCCCCEEEE
ATGKLWFRVPETFRFEVEGKLPERVYSKDLILHLIGDVGVEGVRYMAAEYAGSTIRSLSI
EECEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEECCC
PERMTMSNMAIEMGGKAGIIEADEVTEAYLKERIPGFKLDPYWKSDEGAKYLDIRYYDVS
CCHHHHHHEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECC
DLEPQVACPHNVDNVKPVSEVEGTKLDQIFMGSCTNGRFEDIKIMADIMGDEPVAKNLRL
CCCCCEECCCCCCCCCCHHHCCCCEECEEEECCCCCCCCHHEEEEEECCCCCCCCCCCEE
LVVPASKTEYMKLLKAGYIEKLVNAGAIVEAPCCGPCMGGSFGLLGPGEVGLATSNRNFK
EEEECCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCC
GREGSAESFVYLSSPATAGASALTGEITDPRKV
CCCCCCCEEEEEECCCCCCCHHEECCCCCCCCC
>Mature Secondary Structure
MNFISFWDHQLNLFIRKRQLFPMTGMSGSDNRPMTVSEKIFSKASGTPVKAGDFVLANID
CCCHHHHHHEEEEEEEEEEECEECCCCCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEEEE
LAMTHDITGPLAVQGFYEIMRDEEDKKVWDPSKIVIIFDHQVPADSINAAQNHIMLRKFA
EEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
KEQGILNYDVYEGVCHQVLPEKGHVKPGDLIVGSDSHTCAYGSLGAFSTGIGSTDMAAVF
HHCCCEEEHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCHHCCCCCCCCEEEE
ATGKLWFRVPETFRFEVEGKLPERVYSKDLILHLIGDVGVEGVRYMAAEYAGSTIRSLSI
EECEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEECCC
PERMTMSNMAIEMGGKAGIIEADEVTEAYLKERIPGFKLDPYWKSDEGAKYLDIRYYDVS
CCHHHHHHEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECC
DLEPQVACPHNVDNVKPVSEVEGTKLDQIFMGSCTNGRFEDIKIMADIMGDEPVAKNLRL
CCCCCEECCCCCCCCCCHHHCCCCEECEEEECCCCCCCCHHEEEEEECCCCCCCCCCCEE
LVVPASKTEYMKLLKAGYIEKLVNAGAIVEAPCCGPCMGGSFGLLGPGEVGLATSNRNFK
EEEECCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCC
GREGSAESFVYLSSPATAGASALTGEITDPRKV
CCCCCCCEEEEEECCCCCCCHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]