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The map label for this gene is alsS [H]

Identifier: 21228442

GI number: 21228442

Start: 2796352

End: 2797986

Strand: Reverse

Name: alsS [H]

Synonym: MM_2340

Alternate gene names: 21228442

Gene position: 2797986-2796352 (Counterclockwise)

Preceding gene: 21228443

Following gene: 21228435

Centisome position: 68.3

GC content: 46.91

Gene sequence:

>1635_bases
ATGAAAGCTTCGGATCTCTTTGTTGCCCAGCTTAAAGAGGAAGGTGTGGAATACGTATTCGGACTCCCCGGAGAAGAAAA
CCTTGACCTTCTTGAGTCTCTGCGGAATTCGGATATCAAACTGATAATAACAAGGCATGAACAGGCTGCGGCTTTCATGG
CTGCAGCTTATGGAAGGCTGACAGGGAAGGCAGGTGTCTGCTTTTCAACTCTCGGTCCAGGAGCAACAAATCTTGTTACA
GGCGTTGCTCAGGCGCAGCTTACAGGTGCTCCTCTGATATCTATATCAGGACAGAAAGCCCTGATTGACAACTGGCAGGC
GCGTTTCCAGCTCGTAGATGTTGTCAGGATGATGGAGCCTCTCTGCAAGAAAGCCGTAACGATTTCGGATCCCGGGATGA
TACCTACCGTGCTCCGAAACGCTTTCAAGCATGCAGAGGCTGAAAGGCCCGGAGCGGTACACATTGAACTTCCGGAGGAC
GTAGCTGGAGAAGATACGGACGCAGTTGTGCAGAAGAGGAGTGAGACCAGTATTCCCTCTCCTGACCCCAAAGCCATCGA
AACAGCAGCGGGCATGATTCGCGAGGCTAAAAACCCCCTTATCATAGTCTCTTCAGGAGCTAACCGGAAGGCTGTAACCG
AAGAACTGGAAGATTTTGCAAACAGGACAGGCATTTATGTTGTGCACACACAGATGGGAAAAGGGGTTGTCCCTGACGAC
TGCCCTTACAGCCTTTTTGCAACAGGAATCCATGCAAGGGACTATGTAAACTGTGGAATTGACGGGGCAGACCTGATAAT
AACAGTAGGCTATGATATTGTGGAATATCCACCCTACCTCTGGAATGGAAAGCTGGACAAGAAAATAGTAAATATTGATT
TTGTGGAATCTGTTCCTGACAGGTACTTTAACCCTGCAGTTGAGGTGATAGGAGATATTTCTTCTTCTGTCCGGCAGCTT
GCCGCAAAAGTTCCCGAAAAGAGGGAGTTTCCCGTATTTGAAAACACAAGAACCTTTATCGAAGAAAAAATAAATTCACC
CGTAAAGAAAAGCTATCCTCCCATCCCCCAGGAAGTTGTAAAGAGTGTGAGAGAGGTTCTGGGGCGTGAAGACATCATCT
CTCTTGACAACGGCATATATAAACTGTGGTTTTCCAGGCTTTACAGGGCTTATGCTCCCAATACGGTGCTGCTGGATAAT
GCCCTTGCAACAATGGGCGCAGGCTTTTCTGCAGGGCTTGTTGCAAAACTTCTCCACCCGGAGCGCCGGATACTTGCTGT
CTGCGGAGACGGTGGTTTTATAATGAACTGCCAGGAGCTTGAGACTGCAGTTCGCTACGGAATCCCACTGGTCGTCCTTA
TCCTGAACGACAATGCCTTTGGTTTTATTAAATGGAAACAGAAAAAGATGCGCTTTGAAGATTTTGCTCTGGACTACGGA
AACCCTGATTTTGCCCTCCTTGCCGAAAGTTTCGGGGCAACGGGCATAAAAGTAAAAGAAAACGATGATCTTAAAGAGGT
TCTTGAAAAAGCTTTTTCCCTTAACAAGCTGGTAGTTATCGAATGTCCCATTGATTATTCCGTAAACTATGAGACTTTTT
CAATAGAACTCGGAAACCTTGTGTGTAAATTTTGA

Upstream 100 bases:

>100_bases
AGAAAAGCATTAAAAGAATAAACACTGAAAAAATAACATCGAAAGCAAAACCAGATATATCGTATAAAGTTTCAGGATCA
GGTAAAATAGAGGTTTTTTC

Downstream 100 bases:

>100_bases
TTATATAAAAGTAAAAGGTTTTTGAGAAGAGTCCTTAATTAAGGTACCCTTTGCGTTCAAGCCATTCTATCTGGGGCCTT
GTATATTTCCAGATGACATC

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MKASDLFVAQLKEEGVEYVFGLPGEENLDLLESLRNSDIKLIITRHEQAAAFMAAAYGRLTGKAGVCFSTLGPGATNLVT
GVAQAQLTGAPLISISGQKALIDNWQARFQLVDVVRMMEPLCKKAVTISDPGMIPTVLRNAFKHAEAERPGAVHIELPED
VAGEDTDAVVQKRSETSIPSPDPKAIETAAGMIREAKNPLIIVSSGANRKAVTEELEDFANRTGIYVVHTQMGKGVVPDD
CPYSLFATGIHARDYVNCGIDGADLIITVGYDIVEYPPYLWNGKLDKKIVNIDFVESVPDRYFNPAVEVIGDISSSVRQL
AAKVPEKREFPVFENTRTFIEEKINSPVKKSYPPIPQEVVKSVREVLGREDIISLDNGIYKLWFSRLYRAYAPNTVLLDN
ALATMGAGFSAGLVAKLLHPERRILAVCGDGGFIMNCQELETAVRYGIPLVVLILNDNAFGFIKWKQKKMRFEDFALDYG
NPDFALLAESFGATGIKVKENDDLKEVLEKAFSLNKLVVIECPIDYSVNYETFSIELGNLVCKF

Sequences:

>Translated_544_residues
MKASDLFVAQLKEEGVEYVFGLPGEENLDLLESLRNSDIKLIITRHEQAAAFMAAAYGRLTGKAGVCFSTLGPGATNLVT
GVAQAQLTGAPLISISGQKALIDNWQARFQLVDVVRMMEPLCKKAVTISDPGMIPTVLRNAFKHAEAERPGAVHIELPED
VAGEDTDAVVQKRSETSIPSPDPKAIETAAGMIREAKNPLIIVSSGANRKAVTEELEDFANRTGIYVVHTQMGKGVVPDD
CPYSLFATGIHARDYVNCGIDGADLIITVGYDIVEYPPYLWNGKLDKKIVNIDFVESVPDRYFNPAVEVIGDISSSVRQL
AAKVPEKREFPVFENTRTFIEEKINSPVKKSYPPIPQEVVKSVREVLGREDIISLDNGIYKLWFSRLYRAYAPNTVLLDN
ALATMGAGFSAGLVAKLLHPERRILAVCGDGGFIMNCQELETAVRYGIPLVVLILNDNAFGFIKWKQKKMRFEDFALDYG
NPDFALLAESFGATGIKVKENDDLKEVLEKAFSLNKLVVIECPIDYSVNYETFSIELGNLVCKF
>Mature_544_residues
MKASDLFVAQLKEEGVEYVFGLPGEENLDLLESLRNSDIKLIITRHEQAAAFMAAAYGRLTGKAGVCFSTLGPGATNLVT
GVAQAQLTGAPLISISGQKALIDNWQARFQLVDVVRMMEPLCKKAVTISDPGMIPTVLRNAFKHAEAERPGAVHIELPED
VAGEDTDAVVQKRSETSIPSPDPKAIETAAGMIREAKNPLIIVSSGANRKAVTEELEDFANRTGIYVVHTQMGKGVVPDD
CPYSLFATGIHARDYVNCGIDGADLIITVGYDIVEYPPYLWNGKLDKKIVNIDFVESVPDRYFNPAVEVIGDISSSVRQL
AAKVPEKREFPVFENTRTFIEEKINSPVKKSYPPIPQEVVKSVREVLGREDIISLDNGIYKLWFSRLYRAYAPNTVLLDN
ALATMGAGFSAGLVAKLLHPERRILAVCGDGGFIMNCQELETAVRYGIPLVVLILNDNAFGFIKWKQKKMRFEDFALDYG
NPDFALLAESFGATGIKVKENDDLKEVLEKAFSLNKLVVIECPIDYSVNYETFSIELGNLVCKF

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=540, Percent_Identity=28.1481481481481, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI21361361, Length=549, Percent_Identity=26.775956284153, Blast_Score=154, Evalue=3e-37,
Organism=Escherichia coli, GI87081685, Length=567, Percent_Identity=27.336860670194, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1790104, Length=535, Percent_Identity=28.785046728972, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1786717, Length=579, Percent_Identity=25.9067357512953, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1787096, Length=520, Percent_Identity=26.1538461538462, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=25.8007117437722, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=545, Percent_Identity=27.8899082568807, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=545, Percent_Identity=27.8899082568807, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=565, Percent_Identity=24.6017699115044, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=28.9473684210526, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=536, Percent_Identity=24.2537313432836, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323163, Length=480, Percent_Identity=24.7916666666667, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=482, Percent_Identity=23.8589211618257, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323073, Length=478, Percent_Identity=23.2217573221757, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320123, Length=532, Percent_Identity=22.7443609022556, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922626, Length=551, Percent_Identity=27.5862068965517, Blast_Score=165, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59865; Mature: 59865

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFVAQLKEEGVEYVFGLPGEENLDLLESLRNSDIKLIITRHEQAAAFMAAAYGRL
CCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHC
TGKAGVCFSTLGPGATNLVTGVAQAQLTGAPLISISGQKALIDNWQARFQLVDVVRMMEP
CCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LCKKAVTISDPGMIPTVLRNAFKHAEAERPGAVHIELPEDVAGEDTDAVVQKRSETSIPS
HHHHCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCC
PDPKAIETAAGMIREAKNPLIIVSSGANRKAVTEELEDFANRTGIYVVHTQMGKGVVPDD
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
CPYSLFATGIHARDYVNCGIDGADLIITVGYDIVEYPPYLWNGKLDKKIVNIDFVESVPD
CCHHHHHCCCCCCHHEECCCCCCCEEEEECCHHHHCCCCCCCCCCCCEEEEEEHHHHCCH
RYFNPAVEVIGDISSSVRQLAAKVPEKREFPVFENTRTFIEEKINSPVKKSYPPIPQEVV
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHH
KSVREVLGREDIISLDNGIYKLWFSRLYRAYAPNTVLLDNALATMGAGFSAGLVAKLLHP
HHHHHHHCCCCEEHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCHHHHHHHHHCC
ERRILAVCGDGGFIMNCQELETAVRYGIPLVVLILNDNAFGFIKWKQKKMRFEDFALDYG
CCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHHCCHHHHEEECC
NPDFALLAESFGATGIKVKENDDLKEVLEKAFSLNKLVVIECPIDYSVNYETFSIELGNL
CCCHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCE
VCKF
EECC
>Mature Secondary Structure
MKASDLFVAQLKEEGVEYVFGLPGEENLDLLESLRNSDIKLIITRHEQAAAFMAAAYGRL
CCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHC
TGKAGVCFSTLGPGATNLVTGVAQAQLTGAPLISISGQKALIDNWQARFQLVDVVRMMEP
CCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LCKKAVTISDPGMIPTVLRNAFKHAEAERPGAVHIELPEDVAGEDTDAVVQKRSETSIPS
HHHHCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCC
PDPKAIETAAGMIREAKNPLIIVSSGANRKAVTEELEDFANRTGIYVVHTQMGKGVVPDD
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
CPYSLFATGIHARDYVNCGIDGADLIITVGYDIVEYPPYLWNGKLDKKIVNIDFVESVPD
CCHHHHHCCCCCCHHEECCCCCCCEEEEECCHHHHCCCCCCCCCCCCEEEEEEHHHHCCH
RYFNPAVEVIGDISSSVRQLAAKVPEKREFPVFENTRTFIEEKINSPVKKSYPPIPQEVV
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHH
KSVREVLGREDIISLDNGIYKLWFSRLYRAYAPNTVLLDNALATMGAGFSAGLVAKLLHP
HHHHHHHCCCCEEHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCHHHHHHHHHCC
ERRILAVCGDGGFIMNCQELETAVRYGIPLVVLILNDNAFGFIKWKQKKMRFEDFALDYG
CCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHHCCHHHHEEECC
NPDFALLAESFGATGIKVKENDDLKEVLEKAFSLNKLVVIECPIDYSVNYETFSIELGNL
CCCHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCE
VCKF
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]