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The map label for this gene is ftsJ [H]

Identifier: 21228390

GI number: 21228390

Start: 2727361

End: 2728167

Strand: Direct

Name: ftsJ [H]

Synonym: MM_2288

Alternate gene names: 21228390

Gene position: 2727361-2728167 (Clockwise)

Preceding gene: 21228389

Following gene: 21228391

Centisome position: 66.58

GC content: 45.85

Gene sequence:

>807_bases
ATGGCAAGAGATAGAAGAGATTATTATTATCATCAGGCAAAAGAGGAAGGTTACCGGTCCAGGGCTTCCTTCAAGCTCAA
GCAGATCAATGAGAAACATAATGTTATCAAGCGGGGGGACTCGGTTGTGGATTTAGGTGCAGCTCCGGGTGGGTGGCTTC
AGGTCGCAAAACAGCTTTCCGGAGGGAAGGTCCTCGGTGTTGACCTGCAGAGGATAGACCCTATCGAAGGAGTCGAAACT
ATCCAGGGCGACATAAATGCAGAATCCACGATAAAGAAAATCATTAAAATCGTGGGAGAAAAAGGGGCAGATGTGGTGCT
CTGTGATGCAGCCCCGAACCTGTCAGGTAACTGGTCTTATGACCATGCACGGTCAATTGAGCTTGCAACTTCAGCTCTTG
AATGTGCAAAGAAGATCCTGAAACCAAAAGGAAACTTTGCTGTTAAGGTCTTCCAGGGAGATATGTTCAACGATTACCTT
GACGAAGTAAGAAACAATTTTGTCCGCGTCAAGGCTTACTCCCCTCAGGCGTCAAGGTCGCAGAGTGCGGAGATTTATAT
TATCGGAAAGAAATTCCTTACAGCTCCCCTCAGGAAAGGAGACAAATTCGTGGTGGATATCGAGAAGCTTGGCTCAAGTG
GAGACGGGGCTGTACTTATTGAGGGCTTTGTGGTCTTTGTAAAGGAAGTTGAAGTCGGAGAAAAGGTACGCATAAAAATC
TCGGACGTAAAACCTAACTTTGCATTTGCCGATGTAGAAGAAAGGATTGAAAGCTCTGAAACCGAAAACCGTGAAAAATC
TGACTAA

Upstream 100 bases:

>100_bases
CAAAAAAGATTCAGGTATTATTAATAAAAGATTAAGTGAATAATTGACAAAACGATTTCTGCGCAAGCTATATGAGCTTT
CAGTGCAGGGAAGGTAAGCA

Downstream 100 bases:

>100_bases
CCTCACGATGTAAGGATATGGGACAGGCTCTTATGGTTTTAGTAAGTATTATTGTGCCAGAACCATATCTCATGAGGAAA
GTATATATCTGTAAGGGAAA

Product: methyltransferase

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MARDRRDYYYHQAKEEGYRSRASFKLKQINEKHNVIKRGDSVVDLGAAPGGWLQVAKQLSGGKVLGVDLQRIDPIEGVET
IQGDINAESTIKKIIKIVGEKGADVVLCDAAPNLSGNWSYDHARSIELATSALECAKKILKPKGNFAVKVFQGDMFNDYL
DEVRNNFVRVKAYSPQASRSQSAEIYIIGKKFLTAPLRKGDKFVVDIEKLGSSGDGAVLIEGFVVFVKEVEVGEKVRIKI
SDVKPNFAFADVEERIESSETENREKSD

Sequences:

>Translated_268_residues
MARDRRDYYYHQAKEEGYRSRASFKLKQINEKHNVIKRGDSVVDLGAAPGGWLQVAKQLSGGKVLGVDLQRIDPIEGVET
IQGDINAESTIKKIIKIVGEKGADVVLCDAAPNLSGNWSYDHARSIELATSALECAKKILKPKGNFAVKVFQGDMFNDYL
DEVRNNFVRVKAYSPQASRSQSAEIYIIGKKFLTAPLRKGDKFVVDIEKLGSSGDGAVLIEGFVVFVKEVEVGEKVRIKI
SDVKPNFAFADVEERIESSETENREKSD
>Mature_267_residues
ARDRRDYYYHQAKEEGYRSRASFKLKQINEKHNVIKRGDSVVDLGAAPGGWLQVAKQLSGGKVLGVDLQRIDPIEGVETI
QGDINAESTIKKIIKIVGEKGADVVLCDAAPNLSGNWSYDHARSIELATSALECAKKILKPKGNFAVKVFQGDMFNDYLD
EVRNNFVRVKAYSPQASRSQSAEIYIIGKKFLTAPLRKGDKFVVDIEKLGSSGDGAVLIEGFVVFVKEVEVGEKVRIKIS
DVKPNFAFADVEERIESSETENREKSD

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI194097365, Length=205, Percent_Identity=40.4878048780488, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI29029591, Length=212, Percent_Identity=36.3207547169811, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI29029589, Length=212, Percent_Identity=36.3207547169811, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI7019377, Length=203, Percent_Identity=36.4532019704434, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI7110661, Length=212, Percent_Identity=36.3207547169811, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1789569, Length=184, Percent_Identity=45.6521739130435, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71987550, Length=219, Percent_Identity=39.2694063926941, Blast_Score=150, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71987561, Length=219, Percent_Identity=39.2694063926941, Blast_Score=150, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17553860, Length=219, Percent_Identity=39.2694063926941, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17554650, Length=192, Percent_Identity=36.4583333333333, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17553474, Length=199, Percent_Identity=36.1809045226131, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319796, Length=200, Percent_Identity=39, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=36.1809045226131, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321302, Length=95, Percent_Identity=41.0526315789474, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859957, Length=198, Percent_Identity=41.4141414141414, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24647580, Length=203, Percent_Identity=34.9753694581281, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648639, Length=201, Percent_Identity=36.318407960199, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356387, Length=205, Percent_Identity=30.7317073170732, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR015507
- InterPro:   IPR002877
- InterPro:   IPR002792 [H]

Pfam domain/function: PF01728 FtsJ; PF01938 TRAM [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 29744; Mature: 29613

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDRRDYYYHQAKEEGYRSRASFKLKQINEKHNVIKRGDSVVDLGAAPGGWLQVAKQLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCC
GGKVLGVDLQRIDPIEGVETIQGDINAESTIKKIIKIVGEKGADVVLCDAAPNLSGNWSY
CCEEEEEEHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
DHARSIELATSALECAKKILKPKGNFAVKVFQGDMFNDYLDEVRNNFVRVKAYSPQASRS
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCEEEEEEECCCCCCC
QSAEIYIIGKKFLTAPLRKGDKFVVDIEKLGSSGDGAVLIEGFVVFVKEVEVGEKVRIKI
CCCEEEEEEHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEHHHEEEEEHHCCCCEEEEEE
SDVKPNFAFADVEERIESSETENREKSD
ECCCCCCEEHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ARDRRDYYYHQAKEEGYRSRASFKLKQINEKHNVIKRGDSVVDLGAAPGGWLQVAKQLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCC
GGKVLGVDLQRIDPIEGVETIQGDINAESTIKKIIKIVGEKGADVVLCDAAPNLSGNWSY
CCEEEEEEHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
DHARSIELATSALECAKKILKPKGNFAVKVFQGDMFNDYLDEVRNNFVRVKAYSPQASRS
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCEEEEEEECCCCCCC
QSAEIYIIGKKFLTAPLRKGDKFVVDIEKLGSSGDGAVLIEGFVVFVKEVEVGEKVRIKI
CCCEEEEEEHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEHHHEEEEEHHCCCCEEEEEE
SDVKPNFAFADVEERIESSETENREKSD
ECCCCCCEEHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]