| Definition | Methanosarcina mazei Go1 chromosome, complete genome. |
|---|---|
| Accession | NC_003901 |
| Length | 4,096,345 |
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The map label for this gene is tuf [H]
Identifier: 21228366
GI number: 21228366
Start: 2689067
End: 2690335
Strand: Reverse
Name: tuf [H]
Synonym: MM_2264
Alternate gene names: 21228366
Gene position: 2690335-2689067 (Counterclockwise)
Preceding gene: 21228367
Following gene: 21228365
Centisome position: 65.68
GC content: 50.51
Gene sequence:
>1269_bases ATGGCAGCAGACAAACCGCACATGAATTTAGCAGTGATTGGTCACATTGACCACGGAAAGTCAACATTCGTAGGACGTTT AATGTATGATGCAGGAGCTGTACCTGCTCACATTATCGAGAAGTACAAGGAAGAAGCAAAACAGAAGGGTAAGGAATCCT TCGCTTTTGCATGGGTTATGGACTCTCTTAAGGAAGAGCGTGAAAGAGGTATCACAATCGACATCGCTCACAAGAGATTC GACACAGACAAATTCTACTTTACAGTCGTGGACTGCCCTGGCCACCGTGACTTCGTTAAGAACATGATTACAGGCGCTTC CCAGGCTGATGCAGCTGTTCTCGTTGTCGCAGCTCCTGATGGTGTCATGGCCCAGACCAAGGAACACATCTTCCTTTCCA GGACACTCGGCATCAACCAGCTTATTGTTGCAATCAACAAGATGGATGCAGTAGACTACAGCGAAGCAAGGTACAAGGAA GTCGTTGAGCAGGTATCCGGTATCCTTAAGATGATCGGGTTCAAGCCAAGCGAAATCCCCTTCATCCCGACCTCTGCATT CCACGGTGACAACATTATGAAGCTCAGCGACAAGACTCCCTGGTACAAGGGCCCGGCAATCATGGAAGCCCTCAACAGCC TTAAGGAGCCTGAAAAGCCGTCCACTCTCCCTCTCAGGATCCCTGTCGAAGACGCATACACTATCTCCGGTATCGGAACT GTGCCTGTAGGCAGAGTTGAAACCGGTGTAATGAAGAAGGGTGACAAGGTAGTCTTCATGCCCGGCGGAGCCGGCGGTGA AGTTAAGTCCATTGAAATGCACCATGAAGAAATTCCACAGGCAACCCCTGGAGACAACATCGGCTGGAACGTCCGTGGTA TCGGAAAGAACGACGTCCGCAGAGGAGACGTTTGTGGCCATGCTGACAACCCACCAAAGGTAGCTGACGAGTTTGTCGGA CAGATCGTGGTCCTTCAGCACCCCTCAGCAATCACTGCAGGTTACACACCTGTTTTCCACGCCCACACCTCCCAGATCGC ATGCCAGCTGATCGCTCTTAACAAGAAGCTGGACCCGAAGACTGGCCAGGTTAAGGAAGAAAACCCGACTTTCCTTAAGG CAGGAGATGCAGCAATCGTTACCATCAAGCCAACAAAGCCGATGGTCATCGAGCCCGTAAAAGAAATTCCACAGCTCGGC AGGTTCGCTATCCGTGATATGGGTATGACAATTGCCGCAGGCATGTGCATGAGTGTTAAGCAGAAATAA
Upstream 100 bases:
>100_bases CGGAGAAGGTCTGATCATTACGAGATCTGCCCTAATATTAATATACTATGATTAGATATAGCATTGAAACTCATCCAGAA TTTATAAAGGAGAATAATAA
Downstream 100 bases:
>100_bases CACTCTCCTTTTTTCATTTTTTAAGGAGAAAAAAGGTTATGCAAAAAGCTAGAATAAGATTGTCAGGCATCAGTCCCAAG GATCTGGACGGAGTCTGCAA
Product: elongation factor 1-alpha
Products: GDP; phosphate
Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]
Number of amino acids: Translated: 422; Mature: 421
Protein sequence:
>422_residues MAADKPHMNLAVIGHIDHGKSTFVGRLMYDAGAVPAHIIEKYKEEAKQKGKESFAFAWVMDSLKEERERGITIDIAHKRF DTDKFYFTVVDCPGHRDFVKNMITGASQADAAVLVVAAPDGVMAQTKEHIFLSRTLGINQLIVAINKMDAVDYSEARYKE VVEQVSGILKMIGFKPSEIPFIPTSAFHGDNIMKLSDKTPWYKGPAIMEALNSLKEPEKPSTLPLRIPVEDAYTISGIGT VPVGRVETGVMKKGDKVVFMPGGAGGEVKSIEMHHEEIPQATPGDNIGWNVRGIGKNDVRRGDVCGHADNPPKVADEFVG QIVVLQHPSAITAGYTPVFHAHTSQIACQLIALNKKLDPKTGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLG RFAIRDMGMTIAAGMCMSVKQK
Sequences:
>Translated_422_residues MAADKPHMNLAVIGHIDHGKSTFVGRLMYDAGAVPAHIIEKYKEEAKQKGKESFAFAWVMDSLKEERERGITIDIAHKRF DTDKFYFTVVDCPGHRDFVKNMITGASQADAAVLVVAAPDGVMAQTKEHIFLSRTLGINQLIVAINKMDAVDYSEARYKE VVEQVSGILKMIGFKPSEIPFIPTSAFHGDNIMKLSDKTPWYKGPAIMEALNSLKEPEKPSTLPLRIPVEDAYTISGIGT VPVGRVETGVMKKGDKVVFMPGGAGGEVKSIEMHHEEIPQATPGDNIGWNVRGIGKNDVRRGDVCGHADNPPKVADEFVG QIVVLQHPSAITAGYTPVFHAHTSQIACQLIALNKKLDPKTGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLG RFAIRDMGMTIAAGMCMSVKQK >Mature_421_residues AADKPHMNLAVIGHIDHGKSTFVGRLMYDAGAVPAHIIEKYKEEAKQKGKESFAFAWVMDSLKEERERGITIDIAHKRFD TDKFYFTVVDCPGHRDFVKNMITGASQADAAVLVVAAPDGVMAQTKEHIFLSRTLGINQLIVAINKMDAVDYSEARYKEV VEQVSGILKMIGFKPSEIPFIPTSAFHGDNIMKLSDKTPWYKGPAIMEALNSLKEPEKPSTLPLRIPVEDAYTISGIGTV PVGRVETGVMKKGDKVVFMPGGAGGEVKSIEMHHEEIPQATPGDNIGWNVRGIGKNDVRRGDVCGHADNPPKVADEFVGQ IVVLQHPSAITAGYTPVFHAHTSQIACQLIALNKKLDPKTGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLGR FAIRDMGMTIAAGMCMSVKQK
Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]
COG id: COG5256
COG function: function code J; Translation elongation factor EF-1alpha (GTPase)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]
Homologues:
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=49.3243243243243, Blast_Score=466, Evalue=1e-131, Organism=Homo sapiens, GI4503471, Length=444, Percent_Identity=49.3243243243243, Blast_Score=455, Evalue=1e-128, Organism=Homo sapiens, GI223555963, Length=427, Percent_Identity=37.7049180327869, Blast_Score=314, Evalue=1e-85, Organism=Homo sapiens, GI5729864, Length=427, Percent_Identity=37.7049180327869, Blast_Score=313, Evalue=2e-85, Organism=Homo sapiens, GI194018522, Length=428, Percent_Identity=37.6168224299065, Blast_Score=301, Evalue=9e-82, Organism=Homo sapiens, GI194097354, Length=428, Percent_Identity=37.6168224299065, Blast_Score=301, Evalue=1e-81, Organism=Homo sapiens, GI194018520, Length=428, Percent_Identity=37.6168224299065, Blast_Score=300, Evalue=1e-81, Organism=Homo sapiens, GI46094014, Length=428, Percent_Identity=37.3831775700935, Blast_Score=296, Evalue=2e-80, Organism=Homo sapiens, GI34147630, Length=429, Percent_Identity=32.4009324009324, Blast_Score=197, Evalue=1e-50, Organism=Homo sapiens, GI54607086, Length=335, Percent_Identity=25.6716417910448, Blast_Score=95, Evalue=1e-19, Organism=Homo sapiens, GI82546879, Length=378, Percent_Identity=23.2804232804233, Blast_Score=82, Evalue=1e-15, Organism=Homo sapiens, GI157426893, Length=255, Percent_Identity=28.6274509803922, Blast_Score=80, Evalue=3e-15, Organism=Homo sapiens, GI169162126, Length=144, Percent_Identity=34.7222222222222, Blast_Score=80, Evalue=4e-15, Organism=Homo sapiens, GI169162824, Length=144, Percent_Identity=34.7222222222222, Blast_Score=80, Evalue=4e-15, Organism=Homo sapiens, GI239752724, Length=144, Percent_Identity=34.7222222222222, Blast_Score=80, Evalue=4e-15, Organism=Homo sapiens, GI45593140, Length=394, Percent_Identity=22.5888324873096, Blast_Score=77, Evalue=2e-14, Organism=Homo sapiens, GI53729339, Length=286, Percent_Identity=25.1748251748252, Blast_Score=69, Evalue=7e-12, Organism=Homo sapiens, GI53729337, Length=286, Percent_Identity=25.1748251748252, Blast_Score=69, Evalue=7e-12, Organism=Escherichia coli, GI1789108, Length=424, Percent_Identity=31.6037735849057, Blast_Score=194, Evalue=7e-51, Organism=Escherichia coli, GI1789737, Length=427, Percent_Identity=32.0843091334895, Blast_Score=181, Evalue=8e-47, Organism=Escherichia coli, GI1790412, Length=427, Percent_Identity=32.0843091334895, Blast_Score=181, Evalue=9e-47, Organism=Escherichia coli, GI2367247, Length=375, Percent_Identity=29.8666666666667, Blast_Score=129, Evalue=4e-31, Organism=Escherichia coli, GI48994988, Length=260, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=4e-18, Organism=Escherichia coli, GI1788922, Length=260, Percent_Identity=26.9230769230769, Blast_Score=86, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=49.8871331828442, Blast_Score=465, Evalue=1e-131, Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=49.8871331828442, Blast_Score=465, Evalue=1e-131, Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=47.4770642201835, Blast_Score=429, Evalue=1e-120, Organism=Caenorhabditis elegans, GI32566629, Length=428, Percent_Identity=37.1495327102804, Blast_Score=289, Evalue=2e-78, Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=37.0023419203747, Blast_Score=275, Evalue=3e-74, Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=37.0023419203747, Blast_Score=274, Evalue=8e-74, Organism=Caenorhabditis elegans, GI17556456, Length=431, Percent_Identity=33.1786542923434, Blast_Score=195, Evalue=4e-50, Organism=Caenorhabditis elegans, GI32566301, Length=234, Percent_Identity=42.3076923076923, Blast_Score=176, Evalue=3e-44, Organism=Caenorhabditis elegans, GI25141371, Length=308, Percent_Identity=34.7402597402597, Blast_Score=171, Evalue=6e-43, Organism=Caenorhabditis elegans, GI17557151, Length=250, Percent_Identity=30.4, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17565438, Length=385, Percent_Identity=22.5974025974026, Blast_Score=81, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17506081, Length=294, Percent_Identity=23.469387755102, Blast_Score=79, Evalue=5e-15, Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=50, Blast_Score=75, Evalue=6e-14, Organism=Caenorhabditis elegans, GI71994658, Length=291, Percent_Identity=25.4295532646048, Blast_Score=66, Evalue=4e-11, Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=48.9795918367347, Blast_Score=462, Evalue=1e-131, Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=48.9795918367347, Blast_Score=462, Evalue=1e-131, Organism=Saccharomyces cerevisiae, GI6320377, Length=432, Percent_Identity=35.8796296296296, Blast_Score=277, Evalue=2e-75, Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=31.9383259911894, Blast_Score=219, Evalue=7e-58, Organism=Saccharomyces cerevisiae, GI6324761, Length=428, Percent_Identity=33.1775700934579, Blast_Score=203, Evalue=4e-53, Organism=Saccharomyces cerevisiae, GI6323320, Length=271, Percent_Identity=29.8892988929889, Blast_Score=88, Evalue=3e-18, Organism=Saccharomyces cerevisiae, GI6323098, Length=237, Percent_Identity=29.535864978903, Blast_Score=72, Evalue=2e-13, Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=31.6455696202532, Blast_Score=67, Evalue=6e-12, Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=31.6455696202532, Blast_Score=67, Evalue=6e-12, Organism=Saccharomyces cerevisiae, GI6322359, Length=142, Percent_Identity=33.8028169014084, Blast_Score=65, Evalue=2e-11, Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=49.7747747747748, Blast_Score=462, Evalue=1e-130, Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=49.7747747747748, Blast_Score=462, Evalue=1e-130, Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=49.0990990990991, Blast_Score=457, Evalue=1e-129, Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=49.0990990990991, Blast_Score=457, Evalue=1e-129, Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=49.0990990990991, Blast_Score=457, Evalue=1e-129, Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=49.0990990990991, Blast_Score=457, Evalue=1e-129, Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=38.3908045977011, Blast_Score=303, Evalue=1e-82, Organism=Drosophila melanogaster, GI17137380, Length=427, Percent_Identity=36.768149882904, Blast_Score=291, Evalue=5e-79, Organism=Drosophila melanogaster, GI281363316, Length=422, Percent_Identity=33.6492890995261, Blast_Score=214, Evalue=1e-55, Organism=Drosophila melanogaster, GI17864358, Length=422, Percent_Identity=33.6492890995261, Blast_Score=214, Evalue=1e-55, Organism=Drosophila melanogaster, GI19921738, Length=418, Percent_Identity=29.6650717703349, Blast_Score=178, Evalue=7e-45, Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=26.0586319218241, Blast_Score=99, Evalue=6e-21, Organism=Drosophila melanogaster, GI19922512, Length=427, Percent_Identity=22.248243559719, Blast_Score=86, Evalue=4e-17, Organism=Drosophila melanogaster, GI24654852, Length=427, Percent_Identity=22.248243559719, Blast_Score=86, Evalue=4e-17, Organism=Drosophila melanogaster, GI28572034, Length=291, Percent_Identity=29.2096219931272, Blast_Score=77, Evalue=3e-14, Organism=Drosophila melanogaster, GI78706572, Length=179, Percent_Identity=31.8435754189944, Blast_Score=74, Evalue=3e-13, Organism=Drosophila melanogaster, GI24647048, Length=354, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=3e-13, Organism=Drosophila melanogaster, GI24647046, Length=354, Percent_Identity=24.0112994350282, Blast_Score=73, Evalue=4e-13,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000795 - InterPro: IPR005225 - InterPro: IPR009001 - InterPro: IPR004539 - InterPro: IPR004161 - InterPro: IPR004160 - InterPro: IPR009000 [H]
Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]
EC number: 3.6.5.3
Molecular weight: Translated: 46061; Mature: 45930
Theoretical pI: Translated: 7.74; Mature: 7.74
Prosite motif: PS00301 EFACTOR_GTP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 4.3 %Met (Translated Protein) 5.2 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 4.0 %Met (Mature Protein) 5.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAADKPHMNLAVIGHIDHGKSTFVGRLMYDAGAVPAHIIEKYKEEAKQKGKESFAFAWVM CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHH DSLKEERERGITIDIAHKRFDTDKFYFTVVDCPGHRDFVKNMITGASQADAAVLVVAAPD HHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCC GVMAQTKEHIFLSRTLGINQLIVAINKMDAVDYSEARYKEVVEQVSGILKMIGFKPSEIP CCHHHHHHHEEEEECCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC FIPTSAFHGDNIMKLSDKTPWYKGPAIMEALNSLKEPEKPSTLPLRIPVEDAYTISGIGT CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCC VPVGRVETGVMKKGDKVVFMPGGAGGEVKSIEMHHEEIPQATPGDNIGWNVRGIGKNDVR CCCCCCCHHHHCCCCEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCCCEEEEECCCCCCC RGDVCGHADNPPKVADEFVGQIVVLQHPSAITAGYTPVFHAHTSQIACQLIALNKKLDPK CCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCEEECCHHHHHEEEEEECCCCCCC TGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLGRFAIRDMGMTIAAGMCMSVK CCCCCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC QK CC >Mature Secondary Structure AADKPHMNLAVIGHIDHGKSTFVGRLMYDAGAVPAHIIEKYKEEAKQKGKESFAFAWVM CCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHH DSLKEERERGITIDIAHKRFDTDKFYFTVVDCPGHRDFVKNMITGASQADAAVLVVAAPD HHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCC GVMAQTKEHIFLSRTLGINQLIVAINKMDAVDYSEARYKEVVEQVSGILKMIGFKPSEIP CCHHHHHHHEEEEECCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC FIPTSAFHGDNIMKLSDKTPWYKGPAIMEALNSLKEPEKPSTLPLRIPVEDAYTISGIGT CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCC VPVGRVETGVMKKGDKVVFMPGGAGGEVKSIEMHHEEIPQATPGDNIGWNVRGIGKNDVR CCCCCCCHHHHCCCCEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCCCEEEEECCCCCCC RGDVCGHADNPPKVADEFVGQIVVLQHPSAITAGYTPVFHAHTSQIACQLIALNKKLDPK CCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCEEECCHHHHHEEEEEECCCCCCC TGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLGRFAIRDMGMTIAAGMCMSVK CCCCCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC QK CC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: GTP; H2O
Specific reaction: GTP + H2O = GDP + phosphate
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11932238 [H]