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The map label for this gene is hyaA [C]

Identifier: 21228277

GI number: 21228277

Start: 2577889

End: 2579100

Strand: Direct

Name: hyaA [C]

Synonym: MM_2175

Alternate gene names: 21228277

Gene position: 2577889-2579100 (Clockwise)

Preceding gene: 21228275

Following gene: 21228278

Centisome position: 62.93

GC content: 47.44

Gene sequence:

>1212_bases
ATGGTTGAAGACTCTGTACTTGAAAAGTTCAGAAATCTTGATTTCATGAAAATGGACCGCCGAACATTTATAAAAGCCGT
AGGTGTACTGGGAGCTTCTCTTTTTTTACAAACATATAAAAATGACATGGCAAAAGCCCTTGCCGAGCTGCCGGAAACCA
AAGTGGTATGGCTTCATGGAGTGATGGATTCAGGCTGTACAATATCCATGCTGGACGGAGAAAATCCTGATATCGTGGAA
TTTTTACAGCTTTTTAACCTCAATTTGATGTTTCAGGAAGTTTTGATGATGCAGCAGGGGATTTTTGTTGACGGAGAACT
TGCAAACACATCCGAGCTTAATTCTGAAATCCTTCTTGAGGAAATTCTTGAGCACGAAAGAGGCTATATTCTTATTTCGG
AGGGAGCAGTTGCAAACGGGCCGGATGGAACCGGAAAATACATGATGATTGGGGGGGTACCTTACAAAGAAATTTACGAG
AAAGCTGCAAGAAATGCCTCGGTAATCGTTGCAATCGGGCAGTGTGCAACACATAGTGGAGTAAATGCCGCTGAAAGTGA
AATAGATGCCCTTCTGGACCACCGGGGAATCGCTTTTACTCTGCAAGACCCATCAAAAGGGATTGTGGACCTTCTCGGGA
TAGATACTCCTGTAATCAATATCAACGGTTGTCCTGCACATCCTGACTGGGTATTTTTGACCATAGGGGCAATAGCCCTT
GGAAAAATCAGGGTACCTGATGATTTGCCTGAAGTGCTGGACCGGTGGCACCGTCCGAAGGTCTTCTTCCCTCCTGACCA
TACAGTTCACGATAACTGTCCCCGCCGCGGGTACTATGATCGTGGAGAGTTCGAAACAACCGTGGGAGGGCCAAAATGTC
TCTGGAAACTGGGATGCAAAGGTCCCTATTCTCATGCTGATTGCGCCACACGCCACTGGAACGGCCACCTTACCTTCTGC
CCGCAGGCAGGCTCACCATGCATAGGCTGTGTACAGCCCGGTTTTCCGGATAGCACCAGGCCTTTTTTTGTCGAGATTGA
AGATGCCGGGATTGTTGGTACCAATCTCGATACCATAGCAGGCGTTGCAATAGGAGCTGGACTCCTTGCTGCAGGTGCTC
ATGCCTTAAGAAGGACGGTTTTCAAAAAGCCAGAGGAGAAAGAAGGCACTTCCGGGGAAAATGTTCCTGAGGAGACAGGG
GGCAGAGAATAA

Upstream 100 bases:

>100_bases
TTAGAAGGTTCTCCAAAGCCGTAATAGTCTTTTAATATAATAGGACCTATGTTTTATTATATTCTATTTAAAAAATAATT
TTTATTATTGGGGGAGTATA

Downstream 100 bases:

>100_bases
TGGTGCAGGTCACAGTAGACCCCCTTTCAAGAATTGAAGGGCATTACAGGATTAATACTGAAGTCGATGGAAATGGTGTG
ATCACTGATGCCCAGAGCAA

Product: F420-nonreducing hydrogenase III , small subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MVEDSVLEKFRNLDFMKMDRRTFIKAVGVLGASLFLQTYKNDMAKALAELPETKVVWLHGVMDSGCTISMLDGENPDIVE
FLQLFNLNLMFQEVLMMQQGIFVDGELANTSELNSEILLEEILEHERGYILISEGAVANGPDGTGKYMMIGGVPYKEIYE
KAARNASVIVAIGQCATHSGVNAAESEIDALLDHRGIAFTLQDPSKGIVDLLGIDTPVININGCPAHPDWVFLTIGAIAL
GKIRVPDDLPEVLDRWHRPKVFFPPDHTVHDNCPRRGYYDRGEFETTVGGPKCLWKLGCKGPYSHADCATRHWNGHLTFC
PQAGSPCIGCVQPGFPDSTRPFFVEIEDAGIVGTNLDTIAGVAIGAGLLAAGAHALRRTVFKKPEEKEGTSGENVPEETG
GRE

Sequences:

>Translated_403_residues
MVEDSVLEKFRNLDFMKMDRRTFIKAVGVLGASLFLQTYKNDMAKALAELPETKVVWLHGVMDSGCTISMLDGENPDIVE
FLQLFNLNLMFQEVLMMQQGIFVDGELANTSELNSEILLEEILEHERGYILISEGAVANGPDGTGKYMMIGGVPYKEIYE
KAARNASVIVAIGQCATHSGVNAAESEIDALLDHRGIAFTLQDPSKGIVDLLGIDTPVININGCPAHPDWVFLTIGAIAL
GKIRVPDDLPEVLDRWHRPKVFFPPDHTVHDNCPRRGYYDRGEFETTVGGPKCLWKLGCKGPYSHADCATRHWNGHLTFC
PQAGSPCIGCVQPGFPDSTRPFFVEIEDAGIVGTNLDTIAGVAIGAGLLAAGAHALRRTVFKKPEEKEGTSGENVPEETG
GRE
>Mature_403_residues
MVEDSVLEKFRNLDFMKMDRRTFIKAVGVLGASLFLQTYKNDMAKALAELPETKVVWLHGVMDSGCTISMLDGENPDIVE
FLQLFNLNLMFQEVLMMQQGIFVDGELANTSELNSEILLEEILEHERGYILISEGAVANGPDGTGKYMMIGGVPYKEIYE
KAARNASVIVAIGQCATHSGVNAAESEIDALLDHRGIAFTLQDPSKGIVDLLGIDTPVININGCPAHPDWVFLTIGAIAL
GKIRVPDDLPEVLDRWHRPKVFFPPDHTVHDNCPRRGYYDRGEFETTVGGPKCLWKLGCKGPYSHADCATRHWNGHLTFC
PQAGSPCIGCVQPGFPDSTRPFFVEIEDAGIVGTNLDTIAGVAIGAGLLAAGAHALRRTVFKKPEEKEGTSGENVPEETG
GRE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=367, Percent_Identity=29.9727520435967, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1789371, Length=332, Percent_Identity=30.421686746988, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 43999; Mature: 43999

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDSVLEKFRNLDFMKMDRRTFIKAVGVLGASLFLQTYKNDMAKALAELPETKVVWLHG
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VMDSGCTISMLDGENPDIVEFLQLFNLNLMFQEVLMMQQGIFVDGELANTSELNSEILLE
ECCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHH
EILEHERGYILISEGAVANGPDGTGKYMMIGGVPYKEIYEKAARNASVIVAIGQCATHSG
HHHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCC
VNAAESEIDALLDHRGIAFTLQDPSKGIVDLLGIDTPVININGCPAHPDWVFLTIGAIAL
CCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCCEEEECCCCCCCCEEEEEECHHHC
GKIRVPDDLPEVLDRWHRPKVFFPPDHTVHDNCPRRGYYDRGEFETTVGGPKCLWKLGCK
CCEECCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHEECCC
GPYSHADCATRHWNGHLTFCPQAGSPCIGCVQPGFPDSTRPFFVEIEDAGIVGTNLDTIA
CCCCCCCHHHEECCCCEEECCCCCCCCEEECCCCCCCCCCCEEEEEECCCEECCCHHHHH
GVAIGAGLLAAGAHALRRTVFKKPEEKEGTSGENVPEETGGRE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVEDSVLEKFRNLDFMKMDRRTFIKAVGVLGASLFLQTYKNDMAKALAELPETKVVWLHG
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VMDSGCTISMLDGENPDIVEFLQLFNLNLMFQEVLMMQQGIFVDGELANTSELNSEILLE
ECCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHH
EILEHERGYILISEGAVANGPDGTGKYMMIGGVPYKEIYEKAARNASVIVAIGQCATHSG
HHHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCC
VNAAESEIDALLDHRGIAFTLQDPSKGIVDLLGIDTPVININGCPAHPDWVFLTIGAIAL
CCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCCEEEECCCCCCCCEEEEEECHHHC
GKIRVPDDLPEVLDRWHRPKVFFPPDHTVHDNCPRRGYYDRGEFETTVGGPKCLWKLGCK
CCEECCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHEECCC
GPYSHADCATRHWNGHLTFCPQAGSPCIGCVQPGFPDSTRPFFVEIEDAGIVGTNLDTIA
CCCCCCCHHHEECCCCEEECCCCCCCCEEECCCCCCCCCCCEEEEEECCCEECCCHHHHH
GVAIGAGLLAAGAHALRRTVFKKPEEKEGTSGENVPEETGGRE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]