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The map label for this gene is vhtG [C]

Identifier: 21228271

GI number: 21228271

Start: 2571365

End: 2572525

Strand: Direct

Name: vhtG [C]

Synonym: MM_2169

Alternate gene names: 21228271

Gene position: 2571365-2572525 (Clockwise)

Preceding gene: 21228270

Following gene: 21228272

Centisome position: 62.77

GC content: 48.41

Gene sequence:

>1161_bases
GTGGTAGAGATGAGTACTGGAATGAAAAACCTTACCCGTACACTTGAGAGTATGGATTTCTTAAAAATGGACCGCCGCAC
CTTCATGAAGGCGGTAAGCGCTCTGGGTGCAACTGCATTTCTTGGCACCTATCAAACAGAGATTGTAAATGCGCTTGAGT
TTGCAGAGACAAAACTGATCTGGATACACGGTTCAGAATGCACCGGATGTTCAGAATCTCTTTTAAACGGAGGCAATCCT
GATGTTGCACAGGCTCTGACAAAACTCAATGTAAACCTTGCTTACCATGAGACGCTCTGTATGCAGCAGGGAATATGGAA
TGATGGTGAGCTTGTAAACACCTCAGAGCTTAACTCAGAGATTTTACTTGAAGACCTTTACAAAGAAGGAAATTACATCC
TGGTCGTTGAGGGATCAATCCCCAACGGTCCGGACGGATCAGGAAGATACCTGGTTATAGGGAACAAAACGTTCAAGGAG
ACACTAGGAGAGGCTGCAGAGAACGCAAATGCAATAGTTGCAGTCGGTGCATGTGCATGCTGGGGAGGAATTACCTCTGC
AGACAGCGATATTGAAAAGGAGACAGACTACAGAGGAGTTGCTTTCAAAAAGACCGATGCATCAAAAGGCATGCTCAAGG
AACTTGGAATTGACAAGCCGGTAATTAACATACCGGGCTGCCCTGCTCACCCTGACTGGATACTCCTGACACTGGGTGCG
GTAATACTTGGAAAAATAAAGATACCGGACGACCTGCCAGCAGCTCTGGACCAATACGGAAGGCCGAAGCTGTTCTTCCC
GCCGGACCACACAGTGCATGAAAACTGCCCACGCCGCGGGTACTATGACAGAGGAGAGTTTGACGAAGAAGTAGGAGGAG
AAAAGTGCCTCTGGAAACTGGGATGCAAAGCTCCGTATGCACATGCAGACTGTGGAATTAGAAGATGGAACGGATCGGTA
AGCATGTGTACACAGGCAGGAGGACCGTGCATTAACTGTGTAGACCCGGGTTTTCCGGATGCGTCAAGGCCTCTGTATGT
TGAAGCGGAAGACAAGGGAATTGTAGGGGCAAATATTGACACAGTAGCAAAGGTAGCTGTAGGGGCAGCCGCAGTTGCTG
CAGGTGTGCATGCAGTAAGGAGAATGGGAAAAGGAGAGTGA

Upstream 100 bases:

>100_bases
CATTCAAAACATTATCAGTATATTTATATATGCATTTATATAGTTACAGTTAGATTAATCTAAAGCCCCTTTCGACATAT
ATTTTGGGTAAAATCAAGAT

Downstream 100 bases:

>100_bases
GTGAAAAATGGTAAACGTTACAGTTGATCCCTTAACCAGAATTGAAGGACACCAGAGAATTTCAACAGAAGTAGATGCAA
ACGGTGTTATTACAGATGCA

Product: F420-nonreducing hydrogenase II

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MVEMSTGMKNLTRTLESMDFLKMDRRTFMKAVSALGATAFLGTYQTEIVNALEFAETKLIWIHGSECTGCSESLLNGGNP
DVAQALTKLNVNLAYHETLCMQQGIWNDGELVNTSELNSEILLEDLYKEGNYILVVEGSIPNGPDGSGRYLVIGNKTFKE
TLGEAAENANAIVAVGACACWGGITSADSDIEKETDYRGVAFKKTDASKGMLKELGIDKPVINIPGCPAHPDWILLTLGA
VILGKIKIPDDLPAALDQYGRPKLFFPPDHTVHENCPRRGYYDRGEFDEEVGGEKCLWKLGCKAPYAHADCGIRRWNGSV
SMCTQAGGPCINCVDPGFPDASRPLYVEAEDKGIVGANIDTVAKVAVGAAAVAAGVHAVRRMGKGE

Sequences:

>Translated_386_residues
MVEMSTGMKNLTRTLESMDFLKMDRRTFMKAVSALGATAFLGTYQTEIVNALEFAETKLIWIHGSECTGCSESLLNGGNP
DVAQALTKLNVNLAYHETLCMQQGIWNDGELVNTSELNSEILLEDLYKEGNYILVVEGSIPNGPDGSGRYLVIGNKTFKE
TLGEAAENANAIVAVGACACWGGITSADSDIEKETDYRGVAFKKTDASKGMLKELGIDKPVINIPGCPAHPDWILLTLGA
VILGKIKIPDDLPAALDQYGRPKLFFPPDHTVHENCPRRGYYDRGEFDEEVGGEKCLWKLGCKAPYAHADCGIRRWNGSV
SMCTQAGGPCINCVDPGFPDASRPLYVEAEDKGIVGANIDTVAKVAVGAAAVAAGVHAVRRMGKGE
>Mature_386_residues
MVEMSTGMKNLTRTLESMDFLKMDRRTFMKAVSALGATAFLGTYQTEIVNALEFAETKLIWIHGSECTGCSESLLNGGNP
DVAQALTKLNVNLAYHETLCMQQGIWNDGELVNTSELNSEILLEDLYKEGNYILVVEGSIPNGPDGSGRYLVIGNKTFKE
TLGEAAENANAIVAVGACACWGGITSADSDIEKETDYRGVAFKKTDASKGMLKELGIDKPVINIPGCPAHPDWILLTLGA
VILGKIKIPDDLPAALDQYGRPKLFFPPDHTVHENCPRRGYYDRGEFDEEVGGEKCLWKLGCKAPYAHADCGIRRWNGSV
SMCTQAGGPCINCVDPGFPDASRPLYVEAEDKGIVGANIDTVAKVAVGAAAVAAGVHAVRRMGKGE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=372, Percent_Identity=31.4516129032258, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789371, Length=330, Percent_Identity=34.5454545454545, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 41512; Mature: 41512

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEMSTGMKNLTRTLESMDFLKMDRRTFMKAVSALGATAFLGTYQTEIVNALEFAETKLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
WIHGSECTGCSESLLNGGNPDVAQALTKLNVNLAYHETLCMQQGIWNDGELVNTSELNSE
EEECCCCCCHHHHHHCCCCHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCEEEHHHCCHH
ILLEDLYKEGNYILVVEGSIPNGPDGSGRYLVIGNKTFKETLGEAAENANAIVAVGACAC
HHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCEEEEEEHHHH
WGGITSADSDIEKETDYRGVAFKKTDASKGMLKELGIDKPVINIPGCPAHPDWILLTLGA
HCCCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHH
VILGKIKIPDDLPAALDQYGRPKLFFPPDHTVHENCPRRGYYDRGEFDEEVGGEKCLWKL
HHHCCEECCCCCHHHHHHCCCCEEECCCCCCHHCCCCCCCCCCCCCCHHHCCCCHHHHHH
GCKAPYAHADCGIRRWNGSVSMCTQAGGPCINCVDPGFPDASRPLYVEAEDKGIVGANID
CCCCCCCCCCCCEEEECCCEEEHHCCCCCEEECCCCCCCCCCCCEEEEECCCCEECCCHH
TVAKVAVGAAAVAAGVHAVRRMGKGE
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVEMSTGMKNLTRTLESMDFLKMDRRTFMKAVSALGATAFLGTYQTEIVNALEFAETKLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
WIHGSECTGCSESLLNGGNPDVAQALTKLNVNLAYHETLCMQQGIWNDGELVNTSELNSE
EEECCCCCCHHHHHHCCCCHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCEEEHHHCCHH
ILLEDLYKEGNYILVVEGSIPNGPDGSGRYLVIGNKTFKETLGEAAENANAIVAVGACAC
HHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCEEEEEEHHHH
WGGITSADSDIEKETDYRGVAFKKTDASKGMLKELGIDKPVINIPGCPAHPDWILLTLGA
HCCCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHH
VILGKIKIPDDLPAALDQYGRPKLFFPPDHTVHENCPRRGYYDRGEFDEEVGGEKCLWKL
HHHCCEECCCCCHHHHHHCCCCEEECCCCCCHHCCCCCCCCCCCCCCHHHCCCCHHHHHH
GCKAPYAHADCGIRRWNGSVSMCTQAGGPCINCVDPGFPDASRPLYVEAEDKGIVGANID
CCCCCCCCCCCCEEEECCCEEEHHCCCCCEEECCCCCCCCCCCCEEEEECCCCEECCCHH
TVAKVAVGAAAVAAGVHAVRRMGKGE
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]