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The map label for this gene is truB [H]

Identifier: 21228256

GI number: 21228256

Start: 2556353

End: 2557369

Strand: Direct

Name: truB [H]

Synonym: MM_2154

Alternate gene names: 21228256

Gene position: 2556353-2557369 (Clockwise)

Preceding gene: 21228255

Following gene: 21228257

Centisome position: 62.41

GC content: 51.92

Gene sequence:

>1017_bases
ATGTCATCCGCCGGCAAACTGCCATCAGAAGTTGAAAGAACCCTTGTCCGGAAGTCCGGCGCCTGGACAAATCCAGCCTA
CGGCTGTCCTCCGGAAAAGCGCCCCATCCATGAATATATTGAGAAGGGAGTTGTGAATATTGACAAGCCCAGAGGACCTA
CAAGCCATGAAGTTGCAGCCTGGGTAAAAGCCATCCTTGGCGTACACACGGCAGGACACGCAGGCTCTCTTGACCCTAAG
GTTACAGGTCTTCTGCCAACACTGCTCGGAAAAGCTACCCTTGCCGTGCCAGCCCTGCGCCTCTCGGGAAAAGAGTACAT
CTGCCATTTGAAGCTTCACAGGGCTATGCCCCAGAAGCTGGTCCGCCAGGTATGTGAGGAGTTTACTGGCCCTATTTACC
AGATGCCCCCTATCAAGTCTGCTGTTAAGCGCGTTATAAGGGTAAGGACAATCTACCATCTGGAAGTGCTTGAGATCGAG
GGTACGTCTGTGCTCTTCCGTGTTGGGTGTGAAGCAGGGACTTATATCCGGAAACTCTGCCACGATATAGGGCTCGCTCT
GGGCTGCGGCGGACATATGCAGGGGCTCCGCAGGACCAAAGCAGGCCCGTTTACGGAAAAGACGCTTATTACCCTCCACG
AACTGAAGGATGCTTATGTTTTCTGGAAGGAAGACGGGGACGAGTCTGAATTGAGAAGGGTTATAAGGCCGATGGAATCC
GCCGTATCCCACCTCCCCAAAATTATCCTGCGGGACAGTGCAGTGGATGCTATCTGTTCAGGAGCTTCCCTGGCAGTCCC
TGGTATTACAGGTCTCGACTCTAATCTGGCTGAAGGAGAACTCACAGGGCTATTTACTTTAAAAGGGGAACTTGTTGCTC
TTGCAAAGGCTAAAATGACCACTGAGGAGATCCTCAAAGCCTCTGCGGGTATTGCAGCTTCTCCCATCCGTGTACTTATG
GAAGCAGGAACTTATCCAAGAGGCTGGACTAAAAAAGAAGAAAAGGTACAGCTCTGA

Upstream 100 bases:

>100_bases
TGTGTGAACAGGGGGACATCCGTTATTCTGAAGCCTTATAAACACATACTGGTCACAGATCGCTAATTGAATATACCATA
CCCAGGAGTTTAAAATTACC

Downstream 100 bases:

>100_bases
CCTTTCAAATTTCTCAATATCTCTTTTAAAGTTCTCTCTTCTTTCTTTCTCATTCTTTCCTAGAATAAGATTTATATATC
ACTATTCCGTTATGGTATAA

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSSAGKLPSEVERTLVRKSGAWTNPAYGCPPEKRPIHEYIEKGVVNIDKPRGPTSHEVAAWVKAILGVHTAGHAGSLDPK
VTGLLPTLLGKATLAVPALRLSGKEYICHLKLHRAMPQKLVRQVCEEFTGPIYQMPPIKSAVKRVIRVRTIYHLEVLEIE
GTSVLFRVGCEAGTYIRKLCHDIGLALGCGGHMQGLRRTKAGPFTEKTLITLHELKDAYVFWKEDGDESELRRVIRPMES
AVSHLPKIILRDSAVDAICSGASLAVPGITGLDSNLAEGELTGLFTLKGELVALAKAKMTTEEILKASAGIAASPIRVLM
EAGTYPRGWTKKEEKVQL

Sequences:

>Translated_338_residues
MSSAGKLPSEVERTLVRKSGAWTNPAYGCPPEKRPIHEYIEKGVVNIDKPRGPTSHEVAAWVKAILGVHTAGHAGSLDPK
VTGLLPTLLGKATLAVPALRLSGKEYICHLKLHRAMPQKLVRQVCEEFTGPIYQMPPIKSAVKRVIRVRTIYHLEVLEIE
GTSVLFRVGCEAGTYIRKLCHDIGLALGCGGHMQGLRRTKAGPFTEKTLITLHELKDAYVFWKEDGDESELRRVIRPMES
AVSHLPKIILRDSAVDAICSGASLAVPGITGLDSNLAEGELTGLFTLKGELVALAKAKMTTEEILKASAGIAASPIRVLM
EAGTYPRGWTKKEEKVQL
>Mature_337_residues
SSAGKLPSEVERTLVRKSGAWTNPAYGCPPEKRPIHEYIEKGVVNIDKPRGPTSHEVAAWVKAILGVHTAGHAGSLDPKV
TGLLPTLLGKATLAVPALRLSGKEYICHLKLHRAMPQKLVRQVCEEFTGPIYQMPPIKSAVKRVIRVRTIYHLEVLEIEG
TSVLFRVGCEAGTYIRKLCHDIGLALGCGGHMQGLRRTKAGPFTEKTLITLHELKDAYVFWKEDGDESELRRVIRPMESA
VSHLPKIILRDSAVDAICSGASLAVPGITGLDSNLAEGELTGLFTLKGELVALAKAKMTTEEILKASAGIAASPIRVLME
AGTYPRGWTKKEEKVQL

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI215599015, Length=317, Percent_Identity=43.217665615142, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4503337, Length=317, Percent_Identity=43.217665615142, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI21040257, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI2367200, Length=289, Percent_Identity=30.7958477508651, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17553978, Length=316, Percent_Identity=38.6075949367089, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323204, Length=327, Percent_Identity=42.5076452599388, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI281364189, Length=316, Percent_Identity=42.7215189873418, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI281364187, Length=316, Percent_Identity=42.7215189873418, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI281364185, Length=316, Percent_Identity=42.7215189873418, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI281364183, Length=316, Percent_Identity=42.7215189873418, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI62471759, Length=316, Percent_Identity=42.7215189873418, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI17975520, Length=316, Percent_Identity=42.7215189873418, Blast_Score=261, Evalue=7e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR004521 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 36775; Mature: 36644

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAGKLPSEVERTLVRKSGAWTNPAYGCPPEKRPIHEYIEKGVVNIDKPRGPTSHEVAA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCCCHHHHHH
WVKAILGVHTAGHAGSLDPKVTGLLPTLLGKATLAVPALRLSGKEYICHLKLHRAMPQKL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHH
VRQVCEEFTGPIYQMPPIKSAVKRVIRVRTIYHLEVLEIEGTSVLFRVGCEAGTYIRKLC
HHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCHHHHHHH
HDIGLALGCGGHMQGLRRTKAGPFTEKTLITLHELKDAYVFWKEDGDESELRRVIRPMES
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
AVSHLPKIILRDSAVDAICSGASLAVPGITGLDSNLAEGELTGLFTLKGELVALAKAKMT
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHCC
TEEILKASAGIAASPIRVLMEAGTYPRGWTKKEEKVQL
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SSAGKLPSEVERTLVRKSGAWTNPAYGCPPEKRPIHEYIEKGVVNIDKPRGPTSHEVAA
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCCCHHHHHH
WVKAILGVHTAGHAGSLDPKVTGLLPTLLGKATLAVPALRLSGKEYICHLKLHRAMPQKL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHH
VRQVCEEFTGPIYQMPPIKSAVKRVIRVRTIYHLEVLEIEGTSVLFRVGCEAGTYIRKLC
HHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCHHHHHHH
HDIGLALGCGGHMQGLRRTKAGPFTEKTLITLHELKDAYVFWKEDGDESELRRVIRPMES
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
AVSHLPKIILRDSAVDAICSGASLAVPGITGLDSNLAEGELTGLFTLKGELVALAKAKMT
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHCC
TEEILKASAGIAASPIRVLMEAGTYPRGWTKKEEKVQL
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]