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The map label for this gene is hisS [H]

Identifier: 21228163

GI number: 21228163

Start: 2456360

End: 2457595

Strand: Direct

Name: hisS [H]

Synonym: MM_2061

Alternate gene names: 21228163

Gene position: 2456360-2457595 (Clockwise)

Preceding gene: 21228145

Following gene: 21228164

Centisome position: 59.96

GC content: 49.43

Gene sequence:

>1236_bases
ATGTCAGTCAACAGGCCAAGAGGGACCCGGGATTTTTTACCCGCAGATACTGCCCGGAGAAGGTACGTGGAAAGCGTTAT
GCGAAATGTTGTCCTCAACTGGGGCTACAGTGAAATCATTACGCCCACGTTTGAACATCTGGACCTTTTTACCCTTAAGT
CCGGGGAAGGAATTATAGGGGAGCTCTATAACTTCACGGATAAAGGGGGAAGGGAAATGACCCTCAGGCCGGAGCTTACG
GCTCCCGTGATGAGGTTTTACGTTAATGAACTCCAGCCTTTCCCCAAACCTCTTAAACTGTTCTACTTCGAGAATTGTTT
CCGCTACGAACGCCCCCAGAAAGGCCGCTTCAGAGAGTTCTGGCAGTTCGGGGTCGAACTCATAGGGAGCGGGAAATCCG
ACTCCGATGCCGAGGTTATTGCCCTTGCCAGTGCTCTTCTGAAAGCAGTGGGCATAAAGGGGGACATGAAGCTCGGAAAC
CTTGCAGTGATCCGCACGCTTTTGAAAGGGCTTGAACCCGAGATCGTAAGCAATGTCATGAGGCTTGTGGACAAGAAAGA
GTATGCAGGTCTTGAAGCTCTGCTTGATGAGATAGGGGCAGAAGAACAGTTGAAGTCCGACCTCTTCCGCCTTATACACC
TCCAAGGCAGGTACATCCTCCCTGAAGTAAAAGGAATAGTCGGAAACATTCCTGAGCTCGTGAGCTTTGAGAAGACCCTC
AAGCTTCTTGATGCATACGGAGTCGATTACTCTCTTGACTTTGGAATTGCCCGCGGGCTTGATTATTATACAGGCATGGT
TTTTGAAGTCTACGCTGAGGGCCTCGGCGCCCAGAAGCAGGTCTGTGGAGGTGGCTCTTACCAGCTTATCCAGCTTTTTG
GGGGCGGAGATGTGCCTTCTACAGGCTTCGGAATCGGCTTTGACAGGATAATGGAGATCTGCCCTCTCGTACCTCCTGCA
CAGAAAAGTCTTGTGGTGGTCTCAAAACCCGATACTCACCTGGAAGCTGTAAAAGTTGCGACAGAATTGAGGAACTACCT
GCCAGTCCAGATAGACCTTATGGAGCGCAACTTTAAGGCACAGCTCTCCTATGCAAACACCATCAATGCTGACTATGTGA
TAATTGTAGGGGAAAAAGAACTTGAAGCTGGAAAACTGACCCTCAGGGATATGGTTTCCGGAGAACAGGAATTGTTGACG
CTGGAAGAGATTATTGAGAAAATTTCAGGGCAATAA

Upstream 100 bases:

>100_bases
CAAACTCCATGTATTCAGCGGAATATTCTTTCAGAATAATTCTATATTTGATACATATTTGATATTTCTCTAAATTCGAT
TATACCAGGTGTATTAAAAA

Downstream 100 bases:

>100_bases
AACCGATAATTCTCAAAACCGATAATTTTCTTTTCTCTTTCTCCCTTTTTTCATTCCACTATCCTTTCTCTTTTTTCTTC
CTATTTCCCTTATTTTTCTC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSVNRPRGTRDFLPADTARRRYVESVMRNVVLNWGYSEIITPTFEHLDLFTLKSGEGIIGELYNFTDKGGREMTLRPELT
APVMRFYVNELQPFPKPLKLFYFENCFRYERPQKGRFREFWQFGVELIGSGKSDSDAEVIALASALLKAVGIKGDMKLGN
LAVIRTLLKGLEPEIVSNVMRLVDKKEYAGLEALLDEIGAEEQLKSDLFRLIHLQGRYILPEVKGIVGNIPELVSFEKTL
KLLDAYGVDYSLDFGIARGLDYYTGMVFEVYAEGLGAQKQVCGGGSYQLIQLFGGGDVPSTGFGIGFDRIMEICPLVPPA
QKSLVVVSKPDTHLEAVKVATELRNYLPVQIDLMERNFKAQLSYANTINADYVIIVGEKELEAGKLTLRDMVSGEQELLT
LEEIIEKISGQ

Sequences:

>Translated_411_residues
MSVNRPRGTRDFLPADTARRRYVESVMRNVVLNWGYSEIITPTFEHLDLFTLKSGEGIIGELYNFTDKGGREMTLRPELT
APVMRFYVNELQPFPKPLKLFYFENCFRYERPQKGRFREFWQFGVELIGSGKSDSDAEVIALASALLKAVGIKGDMKLGN
LAVIRTLLKGLEPEIVSNVMRLVDKKEYAGLEALLDEIGAEEQLKSDLFRLIHLQGRYILPEVKGIVGNIPELVSFEKTL
KLLDAYGVDYSLDFGIARGLDYYTGMVFEVYAEGLGAQKQVCGGGSYQLIQLFGGGDVPSTGFGIGFDRIMEICPLVPPA
QKSLVVVSKPDTHLEAVKVATELRNYLPVQIDLMERNFKAQLSYANTINADYVIIVGEKELEAGKLTLRDMVSGEQELLT
LEEIIEKISGQ
>Mature_410_residues
SVNRPRGTRDFLPADTARRRYVESVMRNVVLNWGYSEIITPTFEHLDLFTLKSGEGIIGELYNFTDKGGREMTLRPELTA
PVMRFYVNELQPFPKPLKLFYFENCFRYERPQKGRFREFWQFGVELIGSGKSDSDAEVIALASALLKAVGIKGDMKLGNL
AVIRTLLKGLEPEIVSNVMRLVDKKEYAGLEALLDEIGAEEQLKSDLFRLIHLQGRYILPEVKGIVGNIPELVSFEKTLK
LLDAYGVDYSLDFGIARGLDYYTGMVFEVYAEGLGAQKQVCGGGSYQLIQLFGGGDVPSTGFGIGFDRIMEICPLVPPAQ
KSLVVVSKPDTHLEAVKVATELRNYLPVQIDLMERNFKAQLSYANTINADYVIIVGEKELEAGKLTLRDMVSGEQELLTL
EEIIEKISGQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=460, Percent_Identity=28.2608695652174, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI15029520, Length=465, Percent_Identity=26.0215053763441, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1788861, Length=430, Percent_Identity=31.8604651162791, Blast_Score=201, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71993686, Length=467, Percent_Identity=25.6959314775161, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71993693, Length=467, Percent_Identity=25.6959314775161, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=482, Percent_Identity=23.8589211618257, Blast_Score=109, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24643061, Length=469, Percent_Identity=25.7995735607676, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24643059, Length=487, Percent_Identity=25.0513347022587, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 46033; Mature: 45902

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNRPRGTRDFLPADTARRRYVESVMRNVVLNWGYSEIITPTFEHLDLFTLKSGEGIIG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHCEEEEEECCCCHHH
ELYNFTDKGGREMTLRPELTAPVMRFYVNELQPFPKPLKLFYFENCFRYERPQKGRFREF
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
WQFGVELIGSGKSDSDAEVIALASALLKAVGIKGDMKLGNLAVIRTLLKGLEPEIVSNVM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
RLVDKKEYAGLEALLDEIGAEEQLKSDLFRLIHLQGRYILPEVKGIVGNIPELVSFEKTL
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEECHHHHHHHCCCHHHHHHHHHH
KLLDAYGVDYSLDFGIARGLDYYTGMVFEVYAEGLGAQKQVCGGGSYQLIQLFGGGDVPS
HHHHHCCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCC
TGFGIGFDRIMEICPLVPPAQKSLVVVSKPDTHLEAVKVATELRNYLPVQIDLMERNFKA
CCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHH
QLSYANTINADYVIIVGEKELEAGKLTLRDMVSGEQELLTLEEIIEKISGQ
EEEECEECCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVNRPRGTRDFLPADTARRRYVESVMRNVVLNWGYSEIITPTFEHLDLFTLKSGEGIIG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHCEEEEEECCCCHHH
ELYNFTDKGGREMTLRPELTAPVMRFYVNELQPFPKPLKLFYFENCFRYERPQKGRFREF
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
WQFGVELIGSGKSDSDAEVIALASALLKAVGIKGDMKLGNLAVIRTLLKGLEPEIVSNVM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
RLVDKKEYAGLEALLDEIGAEEQLKSDLFRLIHLQGRYILPEVKGIVGNIPELVSFEKTL
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEECHHHHHHHCCCHHHHHHHHHH
KLLDAYGVDYSLDFGIARGLDYYTGMVFEVYAEGLGAQKQVCGGGSYQLIQLFGGGDVPS
HHHHHCCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCC
TGFGIGFDRIMEICPLVPPAQKSLVVVSKPDTHLEAVKVATELRNYLPVQIDLMERNFKA
CCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHH
QLSYANTINADYVIIVGEKELEAGKLTLRDMVSGEQELLTLEEIIEKISGQ
EEEECEECCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]