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The map label for this gene is mttC

Identifier: 21228149

GI number: 21228149

Start: 2443493

End: 2444149

Strand: Reverse

Name: mttC

Synonym: MM_2047

Alternate gene names: 21228149

Gene position: 2444149-2443493 (Counterclockwise)

Preceding gene: 21228150

Following gene: 21228148

Centisome position: 59.67

GC content: 49.16

Gene sequence:

>657_bases
ATGGCAGGCAAAGAAGAAATTATAGCAAAGGCAAAAAATTCGATTACCGAGTTTGATGAAGAACTTGCAGCAGAAGTCGC
AGAGGAAGCCCTTGCCGCAGGGGTTGACCCTGTAGAGCTGATTGAGAAGGGCTTCACTGCAGGCATGGAAGAAGTCGGCG
AACAGTTTGGACAGGGAGCCCTTTTCCTTCCGCATGTACTTGCAGCTGCTGAAGCCATGAAGGCCGGCATAGAAGTTATC
ACGCCGGAAATGGAGAAGCGCAAGTCTCAGACCAAAAACCTGGGAACGGTCATTATAGGGACTATCGAAGGTGACATCCA
CTCCATCGGAAAAGACATTGTGGCTTCCATGCTGAACATTGCCGGTTTCAAGGTTGTGGATCTTGGAAGAGACGTTGCAA
TTAAGACTTTTGTAGAAAAGGTGAAGGAGCTCAAACCTCAGGTTGTTGCATCCTCTGCACTTATGACTACCACAATGGTT
AACCAGATACAGATAGAGGAACAGCTCAAAGAAGCAGGTGTCCGCGACCAGGTGAAAACCATGGTAGGGGGTGCTCCTGT
AACCCAGGACTGGGCAGATAAGATCGGTGCAGATATCTACGGTGAAAGCGCCAACGATGCAGTCGCCAAGGTAAAAGCAG
CTCTGAAGGTCGGATAA

Upstream 100 bases:

>100_bases
GGTGCCACCAGTAGATGCGGATATCCTCAAGGACATGCAGGCGATTGTGGATAAAGCTGACAGAGCATTCAAAGAGGGAT
AAGAGCAGGGAGGAGTAAAA

Downstream 100 bases:

>100_bases
TCGGAAGAGCGGGCAGTCCCGGGGGCAAAAATAAACCAGAAAGTCCCCCGGCACACCTGCCTGATACTCTAACAAAGGAG
GAGTGGGCATAGATCTGAAA

Product: dimethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 2

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MAGKEEIIAKAKNSITEFDEELAAEVAEEALAAGVDPVELIEKGFTAGMEEVGEQFGQGALFLPHVLAAAEAMKAGIEVI
TPEMEKRKSQTKNLGTVIIGTIEGDIHSIGKDIVASMLNIAGFKVVDLGRDVAIKTFVEKVKELKPQVVASSALMTTTMV
NQIQIEEQLKEAGVRDQVKTMVGGAPVTQDWADKIGADIYGESANDAVAKVKAALKVG

Sequences:

>Translated_218_residues
MAGKEEIIAKAKNSITEFDEELAAEVAEEALAAGVDPVELIEKGFTAGMEEVGEQFGQGALFLPHVLAAAEAMKAGIEVI
TPEMEKRKSQTKNLGTVIIGTIEGDIHSIGKDIVASMLNIAGFKVVDLGRDVAIKTFVEKVKELKPQVVASSALMTTTMV
NQIQIEEQLKEAGVRDQVKTMVGGAPVTQDWADKIGADIYGESANDAVAKVKAALKVG
>Mature_217_residues
AGKEEIIAKAKNSITEFDEELAAEVAEEALAAGVDPVELIEKGFTAGMEEVGEQFGQGALFLPHVLAAAEAMKAGIEVIT
PEMEKRKSQTKNLGTVIIGTIEGDIHSIGKDIVASMLNIAGFKVVDLGRDVAIKTFVEKVKELKPQVVASSALMTTTMVN
QIQIEEQLKEAGVRDQVKTMVGGAPVTQDWADKIGADIYGESANDAVAKVKAALKVG

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain

Homologues:

Organism=Homo sapiens, GI169790923, Length=164, Percent_Identity=29.8780487804878, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1790450, Length=210, Percent_Identity=30.952380952381, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17535405, Length=176, Percent_Identity=31.25, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): MTTC2_METMA (P58983)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634071.1
- ProteinModelPortal:   P58983
- SMR:   P58983
- GeneID:   1480389
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2047
- NMPDR:   fig|192952.1.peg.2047
- HOGENOM:   HBG456121
- OMA:   SITEFDE
- ProtClustDB:   CLSK450002
- BioCyc:   MMAZ192952:MM2047-MONOMER
- GO:   GO:0015948
- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741
- Gene3D:   G3DSA:3.40.50.280
- Gene3D:   G3DSA:1.10.1240.10
- TIGRFAMs:   TIGR02370

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; SSF52242 Cbl-bd; SSF47644 Met_synth_Cbl-bd

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 23065; Mature: 22934

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS51332 B12_BINDING; PS51337 B12_BINDING_NTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKEEIIAKAKNSITEFDEELAAEVAEEALAAGVDPVELIEKGFTAGMEEVGEQFGQGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCC
LFLPHVLAAAEAMKAGIEVITPEMEKRKSQTKNLGTVIIGTIEGDIHSIGKDIVASMLNI
HHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
AGFKVVDLGRDVAIKTFVEKVKELKPQVVASSALMTTTMVNQIQIEEQLKEAGVRDQVKT
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MVGGAPVTQDWADKIGADIYGESANDAVAKVKAALKVG
HHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGKEEIIAKAKNSITEFDEELAAEVAEEALAAGVDPVELIEKGFTAGMEEVGEQFGQGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCC
LFLPHVLAAAEAMKAGIEVITPEMEKRKSQTKNLGTVIIGTIEGDIHSIGKDIVASMLNI
HHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
AGFKVVDLGRDVAIKTFVEKVKELKPQVVASSALMTTTMVNQIQIEEQLKEAGVRDQVKT
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MVGGAPVTQDWADKIGADIYGESANDAVAKVKAALKVG
HHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824