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The map label for this gene is hisI

Identifier: 21228121

GI number: 21228121

Start: 2410777

End: 2411139

Strand: Direct

Name: hisI

Synonym: MM_2019

Alternate gene names: 21228121

Gene position: 2410777-2411139 (Clockwise)

Preceding gene: 21228120

Following gene: 21228122

Centisome position: 58.85

GC content: 46.28

Gene sequence:

>363_bases
ATGATTGACTTTGGTAACCTTAAATATGAAAACGGCTTGATTATTGGCATAGTTCAGGACCGGGCCAGCAAAGAGGTTCT
GATGTGTGCCTACATGAACCGGGAAGCTCTTGAAAAAACGGTTGAAACAGGAATTGCCCATTTCTGGAGCCGCAGCCGGC
AAAAGCTATGGAAAAAAGGTGAAACATCCGGGCACCTGCAAAAAGTAAAGGAAATCAGGATTGATTGCGATATGGATTCC
GTTCTCCTGCTTGTGGAACAGGTTGGAGGGGCCTGCCATATGGGATACAGGTCCTGCTTCTATCGAAATCTTGACGGAGA
AGTTGTGGGTGAAAAGGTGTTTGAGCCTGAGGATGTTTACTGA

Upstream 100 bases:

>100_bases
TTTTCTGTAATAGTGGACACTCCTATTTCCTGATTTTATTCTTTCTGGAGGCAGGTCTTATCCTTCTCCTTTTTTATACT
AGTTTGGCAATTTCTCTCAC

Downstream 100 bases:

>100_bases
ACCTTAATTTATCAAGAGAGATTTTCTCAAATCATAATTAATCAAATTATTATGTTGTATTTTGCTCCTCTTCATCCCTA
TTTTATTCTTTTTTATCACA

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MIDFGNLKYENGLIIGIVQDRASKEVLMCAYMNREALEKTVETGIAHFWSRSRQKLWKKGETSGHLQKVKEIRIDCDMDS
VLLLVEQVGGACHMGYRSCFYRNLDGEVVGEKVFEPEDVY

Sequences:

>Translated_120_residues
MIDFGNLKYENGLIIGIVQDRASKEVLMCAYMNREALEKTVETGIAHFWSRSRQKLWKKGETSGHLQKVKEIRIDCDMDS
VLLLVEQVGGACHMGYRSCFYRNLDGEVVGEKVFEPEDVY
>Mature_120_residues
MIDFGNLKYENGLIIGIVQDRASKEVLMCAYMNREALEKTVETGIAHFWSRSRQKLWKKGETSGHLQKVKEIRIDCDMDS
VLLLVEQVGGACHMGYRSCFYRNLDGEVVGEKVFEPEDVY

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=90, Percent_Identity=52.2222222222222, Blast_Score=101, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319819, Length=91, Percent_Identity=38.4615384615385, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_METMA (Q8PVE6)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634043.1
- ProteinModelPortal:   Q8PVE6
- SMR:   Q8PVE6
- GeneID:   1480361
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2019
- NMPDR:   fig|192952.1.peg.2019
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   CLSK450085
- BioCyc:   MMAZ192952:MM2019-MONOMER
- BRENDA:   3.5.4.19
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 13766; Mature: 13766

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFGNLKYENGLIIGIVQDRASKEVLMCAYMNREALEKTVETGIAHFWSRSRQKLWKKG
CCCCCCEEECCCEEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETSGHLQKVKEIRIDCDMDSVLLLVEQVGGACHMGYRSCFYRNLDGEVVGEKVFEPEDVY
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MIDFGNLKYENGLIIGIVQDRASKEVLMCAYMNREALEKTVETGIAHFWSRSRQKLWKKG
CCCCCCEEECCCEEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETSGHLQKVKEIRIDCDMDSVLLLVEQVGGACHMGYRSCFYRNLDGEVVGEKVFEPEDVY
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824