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The map label for this gene is mdh

Identifier: 21228068

GI number: 21228068

Start: 2335661

End: 2336584

Strand: Reverse

Name: mdh

Synonym: MM_1966

Alternate gene names: 21228068

Gene position: 2336584-2335661 (Counterclockwise)

Preceding gene: 21228072

Following gene: 21228067

Centisome position: 57.04

GC content: 45.45

Gene sequence:

>924_bases
ATGGTTAAAATTTCCGTAATTGGTGCAGGAAATGTAGGTTCGACAACAGTCCAGCGGCTGGCAGAACTGGAGCCTGGCGA
AATTGTCATGACAGATATTGTGGAAGGGATGCCGCAGGGAAAAGCTCTTGACCTTATGCAGGCCGGGGCAATTAACGGTT
ATGACACTAGGATAACAGGTACTAACGATTATGCAGATATCGCAAATTCCGACCTGGTAATTATTACGGCAGGAATCGCC
AGAAAGCCCGGAATGAGCAGGGAAGACCTGATTAAAACGAATTCAAAAATAATAGGGGATGTAGCAGGAAACATTGCGAA
ATACGCTCCGAACTCTATTGTTATAAACGTCACAAATCCGCTTGATATCATAACATATGTAGCTATGAAGGCAACGGGCT
TTGATCCAGAAAAAGTCTTCGGGATGAGTGGAGTTCTTGATGCAGGACGTTTTGCAAGCTTTATAGCAGAGGAACTTAAG
TGTTCAAAAAGGGACGTTGAGGCAATGGTCATAGGAGGGCATGGAGACCTTATGGTTCCGCTCCCGCAGTACACCACAGT
ATCGGGAATCCCGCTTCCGGAACTGCTTCCGGAAAAAACAATTGACAGGCTTGTGGAAAGGACAGTAAATGGAGGAGCAG
AAATAGTAGAGCTTCTCAAACAGGGAAGTGCTTTTTATGCTCCCTCAGCAGCTATCGTACGCATGGCAGAAGCTGTAATT
AAGGACTCCAGGCGCGTACTTCCGGCGTCTGCATATCTTGAAGGACAGTACGGGCAGAAAGGAATTTACTTTGGAGTGCC
AGTAAAACTGGGAGCAAATGGCATAGAAGAAATTCTTGAACTCAAACTTGAAGACAGCCAGTGCGAGATACTCAAAAAAT
CCTCAGAGACTATCCGGAAAGGGATTTCAAAGCTGGAAATCTAA

Upstream 100 bases:

>100_bases
CAAGATATAGTTACCTCCAGACTGATTGAGTAAAACCTATTTTTAGAGGCAGGAATCTGAAAATATCCTGCAAATAAATA
TTGATACAGGTGTACAGGTA

Downstream 100 bases:

>100_bases
AGAGCAAAAATTAAACAGATAAAAATTTGGAGATAAATATTAAAAAGACAGGTTTCTGTTAAGAGAATAGAATATTTTTC
CGGATGGAAAGAATAAATCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MVKISVIGAGNVGSTTVQRLAELEPGEIVMTDIVEGMPQGKALDLMQAGAINGYDTRITGTNDYADIANSDLVIITAGIA
RKPGMSREDLIKTNSKIIGDVAGNIAKYAPNSIVINVTNPLDIITYVAMKATGFDPEKVFGMSGVLDAGRFASFIAEELK
CSKRDVEAMVIGGHGDLMVPLPQYTTVSGIPLPELLPEKTIDRLVERTVNGGAEIVELLKQGSAFYAPSAAIVRMAEAVI
KDSRRVLPASAYLEGQYGQKGIYFGVPVKLGANGIEEILELKLEDSQCEILKKSSETIRKGISKLEI

Sequences:

>Translated_307_residues
MVKISVIGAGNVGSTTVQRLAELEPGEIVMTDIVEGMPQGKALDLMQAGAINGYDTRITGTNDYADIANSDLVIITAGIA
RKPGMSREDLIKTNSKIIGDVAGNIAKYAPNSIVINVTNPLDIITYVAMKATGFDPEKVFGMSGVLDAGRFASFIAEELK
CSKRDVEAMVIGGHGDLMVPLPQYTTVSGIPLPELLPEKTIDRLVERTVNGGAEIVELLKQGSAFYAPSAAIVRMAEAVI
KDSRRVLPASAYLEGQYGQKGIYFGVPVKLGANGIEEILELKLEDSQCEILKKSSETIRKGISKLEI
>Mature_307_residues
MVKISVIGAGNVGSTTVQRLAELEPGEIVMTDIVEGMPQGKALDLMQAGAINGYDTRITGTNDYADIANSDLVIITAGIA
RKPGMSREDLIKTNSKIIGDVAGNIAKYAPNSIVINVTNPLDIITYVAMKATGFDPEKVFGMSGVLDAGRFASFIAEELK
CSKRDVEAMVIGGHGDLMVPLPQYTTVSGIPLPELLPEKTIDRLVERTVNGGAEIVELLKQGSAFYAPSAAIVRMAEAVI
KDSRRVLPASAYLEGQYGQKGIYFGVPVKLGANGIEEILELKLEDSQCEILKKSSETIRKGISKLEI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=34.7540983606557, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=34.7540983606557, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI5031857, Length=311, Percent_Identity=34.0836012861736, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI260099723, Length=311, Percent_Identity=34.0836012861736, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=31.1475409836066, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=31.1475409836066, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI15082234, Length=312, Percent_Identity=31.4102564102564, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI9257228, Length=304, Percent_Identity=30.2631578947368, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4504973, Length=304, Percent_Identity=30.2631578947368, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI260099727, Length=216, Percent_Identity=35.1851851851852, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI260099725, Length=202, Percent_Identity=36.1386138613861, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI207028494, Length=196, Percent_Identity=32.6530612244898, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI21735621, Length=293, Percent_Identity=29.0102389078498, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI103472011, Length=309, Percent_Identity=24.9190938511327, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789632, Length=289, Percent_Identity=32.1799307958478, Blast_Score=110, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17535107, Length=281, Percent_Identity=28.4697508896797, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554310, Length=316, Percent_Identity=31.9620253164557, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322765, Length=249, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320125, Length=346, Percent_Identity=28.3236994219653, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=29.7029702970297, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24647881, Length=243, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI45550422, Length=317, Percent_Identity=26.813880126183, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24663599, Length=246, Percent_Identity=29.6747967479675, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24663595, Length=301, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_METMA (Q8PVJ7)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633990.1
- ProteinModelPortal:   Q8PVJ7
- SMR:   Q8PVJ7
- GeneID:   1480308
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1966
- NMPDR:   fig|192952.1.peg.1966
- HOGENOM:   HBG566126
- OMA:   DAMTYVM
- ProtClustDB:   PRK06223
- BioCyc:   MMAZ192952:MM1966-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 32786; Mature: 32786

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKISVIGAGNVGSTTVQRLAELEPGEIVMTDIVEGMPQGKALDLMQAGAINGYDTRITG
CEEEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEC
TNDYADIANSDLVIITAGIARKPGMSREDLIKTNSKIIGDVAGNIAKYAPNSIVINVTNP
CCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCH
LDIITYVAMKATGFDPEKVFGMSGVLDAGRFASFIAEELKCSKRDVEAMVIGGHGDLMVP
HHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEE
LPQYTTVSGIPLPELLPEKTIDRLVERTVNGGAEIVELLKQGSAFYAPSAAIVRMAEAVI
CCCCCEECCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KDSRRVLPASAYLEGQYGQKGIYFGVPVKLGANGIEEILELKLEDSQCEILKKSSETIRK
HCCCCCCCHHHEEECCCCCCCEEEECCCEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GISKLEI
HHHHCCC
>Mature Secondary Structure
MVKISVIGAGNVGSTTVQRLAELEPGEIVMTDIVEGMPQGKALDLMQAGAINGYDTRITG
CEEEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEC
TNDYADIANSDLVIITAGIARKPGMSREDLIKTNSKIIGDVAGNIAKYAPNSIVINVTNP
CCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCH
LDIITYVAMKATGFDPEKVFGMSGVLDAGRFASFIAEELKCSKRDVEAMVIGGHGDLMVP
HHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEE
LPQYTTVSGIPLPELLPEKTIDRLVERTVNGGAEIVELLKQGSAFYAPSAAIVRMAEAVI
CCCCCEECCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KDSRRVLPASAYLEGQYGQKGIYFGVPVKLGANGIEEILELKLEDSQCEILKKSSETIRK
HCCCCCCCHHHEEECCCCCCCEEEECCCEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GISKLEI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824