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The map label for this gene is lysS [H]

Identifier: 21228018

GI number: 21228018

Start: 2276788

End: 2278323

Strand: Direct

Name: lysS [H]

Synonym: MM_1916

Alternate gene names: 21228018

Gene position: 2276788-2278323 (Clockwise)

Preceding gene: 21228017

Following gene: 21228019

Centisome position: 55.58

GC content: 46.61

Gene sequence:

>1536_bases
ATGACTATGGAAATTAACAACACGGATCCATTTGAAAAAATGCCCCTCCCTGATGATTCCGGTTTATCCGGTTCAGGGGC
TTTTGATGACTCAAAACTTGCAAAATTAAATGGTGTTATAAGCCAGGGCCTCAACCCCTATCCCTATAAATTTGAAAAAG
ATGAAGACATCTGTGAGATCCTGGAAAAATTTGAGGACTTTGAAAAGAACGAAGGATTGACTGTAAGAACTGCTGGAAGG
CTCTACAACATCCGCAAGCACGGAAAGATGATATTTGCCGATCTGGGTGACCAGGCAGGCAGGGTCCAGGTGCTCGTAAG
GAAAGGAAACCTCCCGGACGAGGAATTCGAGATTTTTAAAAATCTTGTAGATTCCGGAGATATAATTGGTGTTCAGGGTG
ATCTTTTCAGGACAAAGAGAGGGGAAAACTCGATCAGCGTTTCGGAATTCTCCCTGCTCTCAAAATCCCTCTGCGCCCTT
CCGGAAAAGTTCCACGGCTTAAAAGATGTTGAAACAAGGTACAGGAAGAGGTACCTCGACCTTATAGTCAATGCCGAAAA
AAGGGAAATTTTTGTTATGAGGAGCAAGCTCATATCGGAAATCAGAAGGTTCCTTACTGACAGGGAATTCCTGGAGTTCG
AGACCCCTATACTCCAGACCGTGTACGGGGGAGCAAATGCGAGGCCTTTCACAACTTTCCACAACTGTCTCGGGCAGAAC
CTTTTTCTGAGAATTGCCCCCGAACTTTACCTCAAGAGGCTTGTTGTCGGCGGGTATGAGAAGGTTTTCGAGATCTGCAA
AAATTTCAGGAACGAAGACATAGACACAACTCACAACCCTGAGTTCACGATGATCGAGGTCTATGAAGCTTACAGGGACT
ATAATGACATGATGGACTTAACCGAATCCCTGGTCTCGGAACTTGTTTTCAAGCTTACAGGCAGTTATGAAGTGCAGATG
GGAGAGAAAACTATCAACCTCCGTTCTCCCTGGAAGAGGATTTCCATGGAGGATGCTTTAAAGGAATATGCCGGGCTTGA
CATCTTTGCCCATTCTATTGAAGACCTGAAGAAAATCGCCATCGAAAACAGGATAGAGGACTACGAGAAAGCAAAGAGCC
ACGGTGAGTTCCTTGCCCTGCTCTTTGAGGGCCTTGTAGAGGACAAGCTGATAGACCCGACTTTCATATACGACTTCCCC
GTAGAAAACTCCCCGCTTGCCAAAAACCACAGGGAGAAAGCAGGTTTTGTCGAGCGTTTTGAGCTTTTCCTTAACGGCTG
GGAGCTGGCAAACGGTTACTCCGAATTAAACGACCCTCTTGAGCAGGAAAAGAGGTTTGAGGAACAGGACAAGAAAAGGA
AACTGGGAGACCTTGAAGCCCAGACCGTCGATTATGACTTCATAAACGCTCTCGGATATGGCCTGCCCCCTACCGGAGGC
ATGGGGCTTGGGATTGACCGCCTTACCATGATCCTTTCCGGTCTCGAATCCATCAAAGAAGTAATCCTCTTCCCGCAGAT
GAAAAGGGAAGACTGA

Upstream 100 bases:

>100_bases
CCTGATATCTATCTGGCAATTTGGCACTGTCCGGTTACGGTTGATTTATATTTCGTTAATTGATTTACTCGTACCACACG
ATGAAGGATGTACTGATTAA

Downstream 100 bases:

>100_bases
TCTGCTTCCGATTTTTTTGTTTTGAAGGTTTGAGCCTGTTTTACTAACTGGACAGGCTTATCGGGGAGAAATCCCCACTT
TTTTATTTCCGGGGCCTGGA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase 2; LysRS 2 [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTMEINNTDPFEKMPLPDDSGLSGSGAFDDSKLAKLNGVISQGLNPYPYKFEKDEDICEILEKFEDFEKNEGLTVRTAGR
LYNIRKHGKMIFADLGDQAGRVQVLVRKGNLPDEEFEIFKNLVDSGDIIGVQGDLFRTKRGENSISVSEFSLLSKSLCAL
PEKFHGLKDVETRYRKRYLDLIVNAEKREIFVMRSKLISEIRRFLTDREFLEFETPILQTVYGGANARPFTTFHNCLGQN
LFLRIAPELYLKRLVVGGYEKVFEICKNFRNEDIDTTHNPEFTMIEVYEAYRDYNDMMDLTESLVSELVFKLTGSYEVQM
GEKTINLRSPWKRISMEDALKEYAGLDIFAHSIEDLKKIAIENRIEDYEKAKSHGEFLALLFEGLVEDKLIDPTFIYDFP
VENSPLAKNHREKAGFVERFELFLNGWELANGYSELNDPLEQEKRFEEQDKKRKLGDLEAQTVDYDFINALGYGLPPTGG
MGLGIDRLTMILSGLESIKEVILFPQMKRED

Sequences:

>Translated_511_residues
MTMEINNTDPFEKMPLPDDSGLSGSGAFDDSKLAKLNGVISQGLNPYPYKFEKDEDICEILEKFEDFEKNEGLTVRTAGR
LYNIRKHGKMIFADLGDQAGRVQVLVRKGNLPDEEFEIFKNLVDSGDIIGVQGDLFRTKRGENSISVSEFSLLSKSLCAL
PEKFHGLKDVETRYRKRYLDLIVNAEKREIFVMRSKLISEIRRFLTDREFLEFETPILQTVYGGANARPFTTFHNCLGQN
LFLRIAPELYLKRLVVGGYEKVFEICKNFRNEDIDTTHNPEFTMIEVYEAYRDYNDMMDLTESLVSELVFKLTGSYEVQM
GEKTINLRSPWKRISMEDALKEYAGLDIFAHSIEDLKKIAIENRIEDYEKAKSHGEFLALLFEGLVEDKLIDPTFIYDFP
VENSPLAKNHREKAGFVERFELFLNGWELANGYSELNDPLEQEKRFEEQDKKRKLGDLEAQTVDYDFINALGYGLPPTGG
MGLGIDRLTMILSGLESIKEVILFPQMKRED
>Mature_510_residues
TMEINNTDPFEKMPLPDDSGLSGSGAFDDSKLAKLNGVISQGLNPYPYKFEKDEDICEILEKFEDFEKNEGLTVRTAGRL
YNIRKHGKMIFADLGDQAGRVQVLVRKGNLPDEEFEIFKNLVDSGDIIGVQGDLFRTKRGENSISVSEFSLLSKSLCALP
EKFHGLKDVETRYRKRYLDLIVNAEKREIFVMRSKLISEIRRFLTDREFLEFETPILQTVYGGANARPFTTFHNCLGQNL
FLRIAPELYLKRLVVGGYEKVFEICKNFRNEDIDTTHNPEFTMIEVYEAYRDYNDMMDLTESLVSELVFKLTGSYEVQMG
EKTINLRSPWKRISMEDALKEYAGLDIFAHSIEDLKKIAIENRIEDYEKAKSHGEFLALLFEGLVEDKLIDPTFIYDFPV
ENSPLAKNHREKAGFVERFELFLNGWELANGYSELNDPLEQEKRFEEQDKKRKLGDLEAQTVDYDFINALGYGLPPTGGM
GLGIDRLTMILSGLESIKEVILFPQMKRED

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=488, Percent_Identity=45.4918032786885, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI194272210, Length=488, Percent_Identity=45.4918032786885, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI45439306, Length=466, Percent_Identity=23.3905579399142, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1790571, Length=484, Percent_Identity=44.8347107438017, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1789256, Length=489, Percent_Identity=44.7852760736196, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=34.5794392523364, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1788173, Length=311, Percent_Identity=27.3311897106109, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=490, Percent_Identity=43.6734693877551, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71994340, Length=490, Percent_Identity=43.6734693877551, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535927, Length=490, Percent_Identity=43.6734693877551, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32566633, Length=299, Percent_Identity=26.4214046822742, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=489, Percent_Identity=44.9897750511247, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=482, Percent_Identity=30.7053941908714, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=470, Percent_Identity=25.1063829787234, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=451, Percent_Identity=23.9467849223947, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640849, Length=491, Percent_Identity=45.4175152749491, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640851, Length=491, Percent_Identity=45.4175152749491, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17136276, Length=469, Percent_Identity=23.2409381663113, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58796; Mature: 58664

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMEINNTDPFEKMPLPDDSGLSGSGAFDDSKLAKLNGVISQGLNPYPYKFEKDEDICEI
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LEKFEDFEKNEGLTVRTAGRLYNIRKHGKMIFADLGDQAGRVQVLVRKGNLPDEEFEIFK
HHHHHHHHCCCCEEEEECCHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHH
NLVDSGDIIGVQGDLFRTKRGENSISVSEFSLLSKSLCALPEKFHGLKDVETRYRKRYLD
HHHCCCCEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHH
LIVNAEKREIFVMRSKLISEIRRFLTDREFLEFETPILQTVYGGANARPFTTFHNCLGQN
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCCC
LFLRIAPELYLKRLVVGGYEKVFEICKNFRNEDIDTTHNPEFTMIEVYEAYRDYNDMMDL
CEEEECHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHH
TESLVSELVFKLTGSYEVQMGEKTINLRSPWKRISMEDALKEYAGLDIFAHSIEDLKKIA
HHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
IENRIEDYEKAKSHGEFLALLFEGLVEDKLIDPTFIYDFPVENSPLAKNHREKAGFVERF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
ELFLNGWELANGYSELNDPLEQEKRFEEQDKKRKLGDLEAQTVDYDFINALGYGLPPTGG
HHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCCC
MGLGIDRLTMILSGLESIKEVILFPQMKRED
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TMEINNTDPFEKMPLPDDSGLSGSGAFDDSKLAKLNGVISQGLNPYPYKFEKDEDICEI
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LEKFEDFEKNEGLTVRTAGRLYNIRKHGKMIFADLGDQAGRVQVLVRKGNLPDEEFEIFK
HHHHHHHHCCCCEEEEECCHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHH
NLVDSGDIIGVQGDLFRTKRGENSISVSEFSLLSKSLCALPEKFHGLKDVETRYRKRYLD
HHHCCCCEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHH
LIVNAEKREIFVMRSKLISEIRRFLTDREFLEFETPILQTVYGGANARPFTTFHNCLGQN
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCCC
LFLRIAPELYLKRLVVGGYEKVFEICKNFRNEDIDTTHNPEFTMIEVYEAYRDYNDMMDL
CEEEECHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHH
TESLVSELVFKLTGSYEVQMGEKTINLRSPWKRISMEDALKEYAGLDIFAHSIEDLKKIA
HHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
IENRIEDYEKAKSHGEFLALLFEGLVEDKLIDPTFIYDFPVENSPLAKNHREKAGFVERF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
ELFLNGWELANGYSELNDPLEQEKRFEEQDKKRKLGDLEAQTVDYDFINALGYGLPPTGG
HHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCCC
MGLGIDRLTMILSGLESIKEVILFPQMKRED
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]