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The map label for this gene is cysS

Identifier: 21228013

GI number: 21228013

Start: 2269793

End: 2271214

Strand: Direct

Name: cysS

Synonym: MM_1911

Alternate gene names: 21228013

Gene position: 2269793-2271214 (Clockwise)

Preceding gene: 21228012

Following gene: 21228014

Centisome position: 55.41

GC content: 45.15

Gene sequence:

>1422_bases
ATGCTGCAAGTCTACAATACCCTGACACGAAATAAAGAGACATTCAAACCTTTAAAGGAAGGCGAGGTTTCGATTTATGC
CTGCGGACCCACAGTCTACAATATGCCTCATATAGGAAATTACCGGACGTTTCTGCTGGCGGATACGGTGGTCAGGACAC
TTCAGTATCTGGGGTATAAAGTAAAGCTCGTAATGAACATAACTGACATAGACGACAAAACTATCAGGGACTCAAAGGCA
GCGGGGATGTCTCTTAAGGATTTTACGGACAAATACTCTGCAGAATTCTTTAAGGGCCTTGATATGCTGAATATAAAACG
GGCTTCAGCCTATCCAAGGGCTACGGAAAATGTTGACGGCATGATTGAGCTTGCACAGAAGCTGATCGAAAAAGGGCTGG
CTTACGAAAAAGGCGGGTCTGTGTATTACAGGATTTCAGGTTTTCCTGACTACGGCAAGCTTTCAAAACTTGATTTTGAC
AGTATCAGGATTGGCGCATCTGTGGATGTGGATGAGTATGATAAGGACAACCCCAGGGACTTTGCTCTCCTGAAAGCCTC
AGCTCCTGAAGAGATTGAAAGAAGCATCTATTATGAAAGCCCATGGGGTAAAATCCGCCCCGGGTGGCATATCGAATGCT
CGGTTATGGCAATGAACAGTTTCGGTCCTACTCTGGACGTCCATATCGGCGGCGTTGACCTTATCTTCCCTCACCATGAA
AATGAGATTGCCCAGTCAGAGGGAGCAACAGGAATACCTTTTGTGCGCCACTGGATTCATGGAGAGCATCTTATAGTTGA
AGGAGAGAAGATGAGCAAATCTAAAGGGAACGTTTTCACCCTACCCGAGATTGTCGAAATGTACGGTGGCGAGGTTGTGC
GTTTCATGTTCCTTTCTGTGCATTATCGGAAAAAACTGGACTATTCCGAAACCTTTGCAGAAAATGCAAAAAATAATTAT
CTGAAACTTAAAGAGACCCTTGACAACCTGGAATTTGCCCTGAAGAATGTGGAAGATGAACCTGGTCCCGGAGACCTGGA
AACCCTTAAAACCCTTCCTGAACTTGAGAATCGGTTCAGGGAAGCCCTTGAGGACGATTTCAATACCCCGAAAGCAATAA
CAGTTTTCAGGGAACTTTCGCGTACAGCCAATGTATACCTTGAGACCGGAAAGAACATGCAGGTTCTGGAAGAGATTCAC
GCCCTGTATAAGCGGTTTTCAGACACTCTTGGCATCTTTGCAAAGGCTGGCGGAGAAGAAGTTCCTGGTGAGGTGGTCAG
GCTTGTTGAAGAGCGCGAAGAAGCCCGGAAAATAAAAGACTGGAAAACTTCTGACGCTATCAGGGAAAAAATAAAGTCAC
TGGGTTATATTGTTCAGGACACAAAAGAGGGGCCAAAAATAAAAAAATCTGAAGAAAGCTGA

Upstream 100 bases:

>100_bases
TTAGATGATATACAGGTAATCTGGCTGATTTAAGGAGATTGGAAGCGTTGAAATGCGCTTTTTTTCATTTTAAGGATCTT
CATTCTCAGAGGCGTTCCAA

Downstream 100 bases:

>100_bases
AAAGGAAAAGCATCAATTATTTATTCAATTATATACTATTTTATTTATCTTATTCACTTTTTCGGGCCGTATTCTCCTTT
TCTCCCATACGTCCCTGTAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MLQVYNTLTRNKETFKPLKEGEVSIYACGPTVYNMPHIGNYRTFLLADTVVRTLQYLGYKVKLVMNITDIDDKTIRDSKA
AGMSLKDFTDKYSAEFFKGLDMLNIKRASAYPRATENVDGMIELAQKLIEKGLAYEKGGSVYYRISGFPDYGKLSKLDFD
SIRIGASVDVDEYDKDNPRDFALLKASAPEEIERSIYYESPWGKIRPGWHIECSVMAMNSFGPTLDVHIGGVDLIFPHHE
NEIAQSEGATGIPFVRHWIHGEHLIVEGEKMSKSKGNVFTLPEIVEMYGGEVVRFMFLSVHYRKKLDYSETFAENAKNNY
LKLKETLDNLEFALKNVEDEPGPGDLETLKTLPELENRFREALEDDFNTPKAITVFRELSRTANVYLETGKNMQVLEEIH
ALYKRFSDTLGIFAKAGGEEVPGEVVRLVEEREEARKIKDWKTSDAIREKIKSLGYIVQDTKEGPKIKKSEES

Sequences:

>Translated_473_residues
MLQVYNTLTRNKETFKPLKEGEVSIYACGPTVYNMPHIGNYRTFLLADTVVRTLQYLGYKVKLVMNITDIDDKTIRDSKA
AGMSLKDFTDKYSAEFFKGLDMLNIKRASAYPRATENVDGMIELAQKLIEKGLAYEKGGSVYYRISGFPDYGKLSKLDFD
SIRIGASVDVDEYDKDNPRDFALLKASAPEEIERSIYYESPWGKIRPGWHIECSVMAMNSFGPTLDVHIGGVDLIFPHHE
NEIAQSEGATGIPFVRHWIHGEHLIVEGEKMSKSKGNVFTLPEIVEMYGGEVVRFMFLSVHYRKKLDYSETFAENAKNNY
LKLKETLDNLEFALKNVEDEPGPGDLETLKTLPELENRFREALEDDFNTPKAITVFRELSRTANVYLETGKNMQVLEEIH
ALYKRFSDTLGIFAKAGGEEVPGEVVRLVEEREEARKIKDWKTSDAIREKIKSLGYIVQDTKEGPKIKKSEES
>Mature_473_residues
MLQVYNTLTRNKETFKPLKEGEVSIYACGPTVYNMPHIGNYRTFLLADTVVRTLQYLGYKVKLVMNITDIDDKTIRDSKA
AGMSLKDFTDKYSAEFFKGLDMLNIKRASAYPRATENVDGMIELAQKLIEKGLAYEKGGSVYYRISGFPDYGKLSKLDFD
SIRIGASVDVDEYDKDNPRDFALLKASAPEEIERSIYYESPWGKIRPGWHIECSVMAMNSFGPTLDVHIGGVDLIFPHHE
NEIAQSEGATGIPFVRHWIHGEHLIVEGEKMSKSKGNVFTLPEIVEMYGGEVVRFMFLSVHYRKKLDYSETFAENAKNNY
LKLKETLDNLEFALKNVEDEPGPGDLETLKTLPELENRFREALEDDFNTPKAITVFRELSRTANVYLETGKNMQVLEEIH
ALYKRFSDTLGIFAKAGGEEVPGEVVRLVEEREEARKIKDWKTSDAIREKIKSLGYIVQDTKEGPKIKKSEES

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=474, Percent_Identity=32.4894514767932, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI62240992, Length=436, Percent_Identity=30.9633027522936, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI303304987, Length=436, Percent_Identity=30.9633027522936, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI21269877, Length=436, Percent_Identity=30.9633027522936, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI10835051, Length=436, Percent_Identity=30.9633027522936, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1786737, Length=480, Percent_Identity=41.875, Blast_Score=338, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17509797, Length=346, Percent_Identity=31.7919075144509, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17509795, Length=346, Percent_Identity=31.7919075144509, Blast_Score=145, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324082, Length=359, Percent_Identity=33.1476323119777, Blast_Score=186, Evalue=7e-48,
Organism=Drosophila melanogaster, GI19922218, Length=401, Percent_Identity=34.9127182044888, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19922364, Length=351, Percent_Identity=35.3276353276353, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_METMA (Q8PVQ1)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633935.1
- ProteinModelPortal:   Q8PVQ1
- SMR:   Q8PVQ1
- GeneID:   1480253
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1911
- NMPDR:   fig|192952.1.peg.1911
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   MMAZ192952:MM1911-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_A
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53862; Mature: 53862

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQVYNTLTRNKETFKPLKEGEVSIYACGPTVYNMPHIGNYRTFLLADTVVRTLQYLGYK
CCCHHHHHHCCHHHHCHHHCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCEE
VKLVMNITDIDDKTIRDSKAAGMSLKDFTDKYSAEFFKGLDMLNIKRASAYPRATENVDG
EEEEEEEECCCCCHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHH
MIELAQKLIEKGLAYEKGGSVYYRISGFPDYGKLSKLDFDSIRIGASVDVDEYDKDNPRD
HHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCCCCEEECCCCCHHCCCCCCCCC
FALLKASAPEEIERSIYYESPWGKIRPGWHIECSVMAMNSFGPTLDVHIGGVDLIFPHHE
EEEEECCCHHHHHHHEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCEEEEECCCC
NEIAQSEGATGIPFVRHWIHGEHLIVEGEKMSKSKGNVFTLPEIVEMYGGEVVRFMFLSV
CHHHHCCCCCCCHHHHHHHCCCEEEEECCHHCCCCCCEEEHHHHHHHHCCHHHHHHHHHH
HYRKKLDYSETFAENAKNNYLKLKETLDNLEFALKNVEDEPGPGDLETLKTLPELENRFR
HHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
EALEDDFNTPKAITVFRELSRTANVYLETGKNMQVLEEIHALYKRFSDTLGIFAKAGGEE
HHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPGEVVRLVEEREEARKIKDWKTSDAIREKIKSLGYIVQDTKEGPKIKKSEES
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLQVYNTLTRNKETFKPLKEGEVSIYACGPTVYNMPHIGNYRTFLLADTVVRTLQYLGYK
CCCHHHHHHCCHHHHCHHHCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCEE
VKLVMNITDIDDKTIRDSKAAGMSLKDFTDKYSAEFFKGLDMLNIKRASAYPRATENVDG
EEEEEEEECCCCCHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHH
MIELAQKLIEKGLAYEKGGSVYYRISGFPDYGKLSKLDFDSIRIGASVDVDEYDKDNPRD
HHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCCCCEEECCCCCHHCCCCCCCCC
FALLKASAPEEIERSIYYESPWGKIRPGWHIECSVMAMNSFGPTLDVHIGGVDLIFPHHE
EEEEECCCHHHHHHHEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCEEEEECCCC
NEIAQSEGATGIPFVRHWIHGEHLIVEGEKMSKSKGNVFTLPEIVEMYGGEVVRFMFLSV
CHHHHCCCCCCCHHHHHHHCCCEEEEECCHHCCCCCCEEEHHHHHHHHCCHHHHHHHHHH
HYRKKLDYSETFAENAKNNYLKLKETLDNLEFALKNVEDEPGPGDLETLKTLPELENRFR
HHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
EALEDDFNTPKAITVFRELSRTANVYLETGKNMQVLEEIHALYKRFSDTLGIFAKAGGEE
HHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPGEVVRLVEEREEARKIKDWKTSDAIREKIKSLGYIVQDTKEGPKIKKSEES
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824