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The map label for this gene is birA [H]

Identifier: 21227931

GI number: 21227931

Start: 2180649

End: 2181626

Strand: Direct

Name: birA [H]

Synonym: MM_1829

Alternate gene names: 21227931

Gene position: 2180649-2181626 (Clockwise)

Preceding gene: 161485671

Following gene: 21227932

Centisome position: 53.23

GC content: 47.14

Gene sequence:

>978_bases
ATGGGAGATAAAAGGTCTCGAATTATTAAGGCACTTAAAGATGCGCAAAAGACTCCGGTTTCTGGAGAAGAGCTGGGGCT
CAAGCTTGGAATTTCCAGGACAATGGTATGGAAATACATTAAATCCCTCCAGGCTGATGGATACGAAATAGAGTCATCTC
CGAAAAGAGGATATGTCCTGAAATCCGTTCCTCAACTCCTGTACCCTGATGAGATTCAGATGGGGCTCAAAACAACTCTC
CTGGGTAAAAAAATTCATTACTTCGAGGAGGTAAGCTCCACCAACAGTGTTGCGAAAGAGATCGCAGCTTCCGAAGAAGA
AGGAGCTCTTGTCATTGCCGAGATACAGAAAGGAGGCAGGGGCCGCATGGGCAGGGAATGGGTCTCCCCTCACGGCGGAA
TATGGATGTCAGTAATACTTAAGCCTGGGATCCCTCTCAGGCATGCCTCAAGGCTGACCCTTGTTGCCGGGCTTGCAGTT
GCAAATGTTATCCGTGATATGGGGCTCGATGCCCGTATCAAGTGGCCAAACGATGTCCGTATAAACGGGAAAAAAGTATG
CGGAATCCTTACCGAAGCAAAGGCTGAAGTAGACAGGGTGGATTATGTTGTCCTCGGTATAGGAATCAATGTAAATATGG
ATTTAAAAGACATCCCCGAATCTTTCCGTGCAGGCTCGACCACCCTTAAAGTGGAACTCGGAAAACACATCAGGAGGGTT
TCTTTCCTTCAGGATTTCCTTTTCGAGCTTGAGCAGCAGTACATAAGCTTCAAAACCCAGCCGTTCTCTCATATTCTCAA
TGACTGGCTCGCCCTCTCGGATACAATTGGTAGGGAGGTAAAAGTCACCACGCCTTCCAGGATTATAGAGGGAAGGGCGG
TTGGAGTAACTCCTGACGGAGCCCTTATCATAAGGAAGGCAGATAATACTAAGGAAGAAATTATTGCAGGTAGATGCATT
TATGCGCGTCCCAAATAA

Upstream 100 bases:

>100_bases
AAAAGAGGAAAAAGATTTTCCGGCTTATCAGGGTATGACCATATGTCTGGACTAAGAGAATATATACGGTGGAACATATA
CATTTGTATGGGGTGCACGG

Downstream 100 bases:

>100_bases
ATGCGAAAAAAAGAGCAGTAAAGGAATGAAATTCATTTCAGCTCTTTTTTTTTCAATCCCGGTCATTTTTATATTTTCAA
TTCTCATTGCCTGTGCAGTC

Product: biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGDKRSRIIKALKDAQKTPVSGEELGLKLGISRTMVWKYIKSLQADGYEIESSPKRGYVLKSVPQLLYPDEIQMGLKTTL
LGKKIHYFEEVSSTNSVAKEIAASEEEGALVIAEIQKGGRGRMGREWVSPHGGIWMSVILKPGIPLRHASRLTLVAGLAV
ANVIRDMGLDARIKWPNDVRINGKKVCGILTEAKAEVDRVDYVVLGIGINVNMDLKDIPESFRAGSTTLKVELGKHIRRV
SFLQDFLFELEQQYISFKTQPFSHILNDWLALSDTIGREVKVTTPSRIIEGRAVGVTPDGALIIRKADNTKEEIIAGRCI
YARPK

Sequences:

>Translated_325_residues
MGDKRSRIIKALKDAQKTPVSGEELGLKLGISRTMVWKYIKSLQADGYEIESSPKRGYVLKSVPQLLYPDEIQMGLKTTL
LGKKIHYFEEVSSTNSVAKEIAASEEEGALVIAEIQKGGRGRMGREWVSPHGGIWMSVILKPGIPLRHASRLTLVAGLAV
ANVIRDMGLDARIKWPNDVRINGKKVCGILTEAKAEVDRVDYVVLGIGINVNMDLKDIPESFRAGSTTLKVELGKHIRRV
SFLQDFLFELEQQYISFKTQPFSHILNDWLALSDTIGREVKVTTPSRIIEGRAVGVTPDGALIIRKADNTKEEIIAGRCI
YARPK
>Mature_324_residues
GDKRSRIIKALKDAQKTPVSGEELGLKLGISRTMVWKYIKSLQADGYEIESSPKRGYVLKSVPQLLYPDEIQMGLKTTLL
GKKIHYFEEVSSTNSVAKEIAASEEEGALVIAEIQKGGRGRMGREWVSPHGGIWMSVILKPGIPLRHASRLTLVAGLAVA
NVIRDMGLDARIKWPNDVRINGKKVCGILTEAKAEVDRVDYVVLGIGINVNMDLKDIPESFRAGSTTLKVELGKHIRRVS
FLQDFLFELEQQYISFKTQPFSHILNDWLALSDTIGREVKVTTPSRIIEGRAVGVTPDGALIIRKADNTKEEIIAGRCIY
ARPK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=289, Percent_Identity=32.5259515570934, Blast_Score=128, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36076; Mature: 35945

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDKRSRIIKALKDAQKTPVSGEELGLKLGISRTMVWKYIKSLQADGYEIESSPKRGYVL
CCCHHHHHHHHHHHHHCCCCCHHHCCEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCHH
KSVPQLLYPDEIQMGLKTTLLGKKIHYFEEVSSTNSVAKEIAASEEEGALVIAEIQKGGR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GRMGREWVSPHGGIWMSVILKPGIPLRHASRLTLVAGLAVANVIRDMGLDARIKWPNDVR
CCCCCHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
INGKKVCGILTEAKAEVDRVDYVVLGIGINVNMDLKDIPESFRAGSTTLKVELGKHIRRV
ECCHHHEEHHHHHHHHHHHEEEEEEEEEEEECCCHHHCCHHHHCCCEEEEEHHHHHHHHH
SFLQDFLFELEQQYISFKTQPFSHILNDWLALSDTIGREVKVTTPSRIIEGRAVGVTPDG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCEEEECCCC
ALIIRKADNTKEEIIAGRCIYARPK
EEEEEECCCCHHHHHCCCEEECCCC
>Mature Secondary Structure 
GDKRSRIIKALKDAQKTPVSGEELGLKLGISRTMVWKYIKSLQADGYEIESSPKRGYVL
CCHHHHHHHHHHHHHCCCCCHHHCCEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCHH
KSVPQLLYPDEIQMGLKTTLLGKKIHYFEEVSSTNSVAKEIAASEEEGALVIAEIQKGGR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GRMGREWVSPHGGIWMSVILKPGIPLRHASRLTLVAGLAVANVIRDMGLDARIKWPNDVR
CCCCCHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
INGKKVCGILTEAKAEVDRVDYVVLGIGINVNMDLKDIPESFRAGSTTLKVELGKHIRRV
ECCHHHEEHHHHHHHHHHHEEEEEEEEEEEECCCHHHCCHHHHCCCEEEEEHHHHHHHHH
SFLQDFLFELEQQYISFKTQPFSHILNDWLALSDTIGREVKVTTPSRIIEGRAVGVTPDG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCEEEECCCC
ALIIRKADNTKEEIIAGRCIYARPK
EEEEEECCCCHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]