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The map label for this gene is pycB [H]

Identifier: 21227929

GI number: 21227929

Start: 2177306

End: 2179027

Strand: Direct

Name: pycB [H]

Synonym: MM_1827

Alternate gene names: 21227929

Gene position: 2177306-2179027 (Clockwise)

Preceding gene: 21227928

Following gene: 161485671

Centisome position: 53.15

GC content: 49.3

Gene sequence:

>1722_bases
ATGCGTGTAAAAATTACTGAAACCGTCCTCCGGGACGCACACCAGTCCCTCCTGGCAACCAGAATGCGGACAAGGGATAT
GCTCGAAGTGGTTGAACAACTGGACCAGATTGGGTATTTTTCCCTCGAGATGTGGGGAGGTGCCACTTTCGACTCCTGTA
TTCGCTATCTTAACGAAGACCCCTGGCAGCGCCTCAGGGACCTTAAGAAAAATATGGACAACACATATGCTCAGATGCTT
CTCCGCGGCCAGAACCTCGTAGGATACAGGCATTATTCGGACGATGTGGTTGAAAAGTTCGTAACCAAGTCCTATGAAAA
CGGCATTGATATATTCCGGGTTTTTGACGCTGTAAATGATGTCAGGAACATGGAGCTTTCCATAAAAGTGGCAAAAAGGC
TGGGAGCTCATGTCCAGGGCACGGTCTGCTACACTATAAGTCCTGTACATACAGTAGAAAAGTATGTAGGACTCGCAAAA
CAGCTTGAAGAGCTGGAATGTGATTCGCTCTGTATCAAAGATATGGCAGGCCTTCTTTCTCCAAATGAAGCGGCAAAAAT
AATCAGTGCCATGAAAAAAGAGGTTTCTATCCCTATTTCTCTCCACTGTCACTGTACTTCGGGAATGGCTCCTATGAGCT
ATATGGCAGCCTGCCCTGCCGGAGTGGATGTTCTGGATACGGCACTCTCCCCTCTGGCATGGGGGACCTCCCAGCCTCCT
ACCGAGACCGTTGTTGCAGCTCTTCAGGGAACCCCCTATGATACAGGACTTGACCTTGGGGCTTTTGAAGAGGCTGTCAG
GTACTTCAAAGACCTGAAAGAAAAGTACAGAGGAATCCTCGATCCTATTTCCGAGCAGATTGACACCAATGTTCTTATCT
ACCAGATCCCGGGAGGAATGCTCTCCAATCTTGTTTCTCAGTTGAAAGAGCAGAATGCCCTCGATAAATACGGGGCAGTG
CTTCAAGAGATGCCAAAGGTCAGAGAAGAGCTCGGTTATCCTCCTCTTGTTACTCCCACAAGCCAGATCGTAGGCACACA
GGCTGTGCTCAATGTTCTCATGGGAGAACGCTACAAGGTCATCCCGAAAGAGGTAAAGGACTACGTACGCGGACTCTACG
GACGTTCTCCAGCCCCGATCAGCCCTGAGATCATAGGAAAGATCATAGGCGATGAAGAACCGATTCACTGCCGCCCTGCA
GACCTTCTCAAGCCTGAGTATGAGAAGAGAAAAAAAGAAGCAGAAGAAATGGGCATTGCAAATTCTGAAGAAGATATCCT
TACATACATACTCTATCCGGCAATTGCTCCCAAGTTCCTGAAAGGGGAAATGGAAGAAGAAGCCCTGGCTGTTATCACCC
CTGCCCCGGCGACTCCTCAGGAATACACAATTCCTACACACTTCAAGGTTGAGGTGGACGATGAGGTCTACGAAGTCAGG
GTCGAGCCTCTAGGCGGCGGAGTTTCCATCTCTGAAGCGTCGCCTAAAAAGCCAAGCGCAGAATCCGTTAAAGGAGGGGT
TACCAGCTCAATGCAGGGCATGGTACTTTCCCTTAAGGTTAAAGTAGAAGATACCGTCCGGGAAGGAGACACCGTAGCTG
TTATCGAAGCCATGAAGATGGAGAATGCGGTTCATGCCCCACGCTCGGGAATAGTTAAGGAAATCTTTGTAGCCGAAGGG
GACACCGTCGCGCCCGGAGATATCATCCTTTCAATCGAGTAA

Upstream 100 bases:

>100_bases
CAGGCTCTGTATGCCCTGAAAACTGGACAGTTTTCTGGAGAATATATAGACGCATGTTTCAATAAATTCTGTTTTTTGAT
CCGATTTAAGAGGCTCATCC

Downstream 100 bases:

>100_bases
ATCGGGAAAGGTGGAAGGTATCATGTTCAAAAAAGTACTCGTTGCAAACCGCGGTGAAATAGCAATCAGGGTTATGCGTG
CCTGCAGGGAGCTCGGGATT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRVKITETVLRDAHQSLLATRMRTRDMLEVVEQLDQIGYFSLEMWGGATFDSCIRYLNEDPWQRLRDLKKNMDNTYAQML
LRGQNLVGYRHYSDDVVEKFVTKSYENGIDIFRVFDAVNDVRNMELSIKVAKRLGAHVQGTVCYTISPVHTVEKYVGLAK
QLEELECDSLCIKDMAGLLSPNEAAKIISAMKKEVSIPISLHCHCTSGMAPMSYMAACPAGVDVLDTALSPLAWGTSQPP
TETVVAALQGTPYDTGLDLGAFEEAVRYFKDLKEKYRGILDPISEQIDTNVLIYQIPGGMLSNLVSQLKEQNALDKYGAV
LQEMPKVREELGYPPLVTPTSQIVGTQAVLNVLMGERYKVIPKEVKDYVRGLYGRSPAPISPEIIGKIIGDEEPIHCRPA
DLLKPEYEKRKKEAEEMGIANSEEDILTYILYPAIAPKFLKGEMEEEALAVITPAPATPQEYTIPTHFKVEVDDEVYEVR
VEPLGGGVSISEASPKKPSAESVKGGVTSSMQGMVLSLKVKVEDTVREGDTVAVIEAMKMENAVHAPRSGIVKEIFVAEG
DTVAPGDIILSIE

Sequences:

>Translated_573_residues
MRVKITETVLRDAHQSLLATRMRTRDMLEVVEQLDQIGYFSLEMWGGATFDSCIRYLNEDPWQRLRDLKKNMDNTYAQML
LRGQNLVGYRHYSDDVVEKFVTKSYENGIDIFRVFDAVNDVRNMELSIKVAKRLGAHVQGTVCYTISPVHTVEKYVGLAK
QLEELECDSLCIKDMAGLLSPNEAAKIISAMKKEVSIPISLHCHCTSGMAPMSYMAACPAGVDVLDTALSPLAWGTSQPP
TETVVAALQGTPYDTGLDLGAFEEAVRYFKDLKEKYRGILDPISEQIDTNVLIYQIPGGMLSNLVSQLKEQNALDKYGAV
LQEMPKVREELGYPPLVTPTSQIVGTQAVLNVLMGERYKVIPKEVKDYVRGLYGRSPAPISPEIIGKIIGDEEPIHCRPA
DLLKPEYEKRKKEAEEMGIANSEEDILTYILYPAIAPKFLKGEMEEEALAVITPAPATPQEYTIPTHFKVEVDDEVYEVR
VEPLGGGVSISEASPKKPSAESVKGGVTSSMQGMVLSLKVKVEDTVREGDTVAVIEAMKMENAVHAPRSGIVKEIFVAEG
DTVAPGDIILSIE
>Mature_573_residues
MRVKITETVLRDAHQSLLATRMRTRDMLEVVEQLDQIGYFSLEMWGGATFDSCIRYLNEDPWQRLRDLKKNMDNTYAQML
LRGQNLVGYRHYSDDVVEKFVTKSYENGIDIFRVFDAVNDVRNMELSIKVAKRLGAHVQGTVCYTISPVHTVEKYVGLAK
QLEELECDSLCIKDMAGLLSPNEAAKIISAMKKEVSIPISLHCHCTSGMAPMSYMAACPAGVDVLDTALSPLAWGTSQPP
TETVVAALQGTPYDTGLDLGAFEEAVRYFKDLKEKYRGILDPISEQIDTNVLIYQIPGGMLSNLVSQLKEQNALDKYGAV
LQEMPKVREELGYPPLVTPTSQIVGTQAVLNVLMGERYKVIPKEVKDYVRGLYGRSPAPISPEIIGKIIGDEEPIHCRPA
DLLKPEYEKRKKEAEEMGIANSEEDILTYILYPAIAPKFLKGEMEEEALAVITPAPATPQEYTIPTHFKVEVDDEVYEVR
VEPLGGGVSISEASPKKPSAESVKGGVTSSMQGMVLSLKVKVEDTVREGDTVAVIEAMKMENAVHAPRSGIVKEIFVAEG
DTVAPGDIILSIE

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=618, Percent_Identity=31.7152103559871, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI106049295, Length=618, Percent_Identity=31.7152103559871, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI106049292, Length=618, Percent_Identity=31.7152103559871, Blast_Score=268, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17562816, Length=619, Percent_Identity=33.1179321486268, Blast_Score=280, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319695, Length=619, Percent_Identity=31.1793214862682, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6321376, Length=620, Percent_Identity=30.6451612903226, Blast_Score=277, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24652212, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24652210, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24652214, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI19921944, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24652216, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI281363050, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24652224, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24652222, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24652220, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24652218, Length=621, Percent_Identity=34.1384863123994, Blast_Score=303, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 63368; Mature: 63368

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKITETVLRDAHQSLLATRMRTRDMLEVVEQLDQIGYFSLEMWGGATFDSCIRYLNED
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCC
PWQRLRDLKKNMDNTYAQMLLRGQNLVGYRHYSDDVVEKFVTKSYENGIDIFRVFDAVND
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VRNMELSIKVAKRLGAHVQGTVCYTISPVHTVEKYVGLAKQLEELECDSLCIKDMAGLLS
HHCCHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
PNEAAKIISAMKKEVSIPISLHCHCTSGMAPMSYMAACPAGVDVLDTALSPLAWGTSQPP
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
TETVVAALQGTPYDTGLDLGAFEEAVRYFKDLKEKYRGILDPISEQIDTNVLIYQIPGGM
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
LSNLVSQLKEQNALDKYGAVLQEMPKVREELGYPPLVTPTSQIVGTQAVLNVLMGERYKV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
IPKEVKDYVRGLYGRSPAPISPEIIGKIIGDEEPIHCRPADLLKPEYEKRKKEAEEMGIA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCC
NSEEDILTYILYPAIAPKFLKGEMEEEALAVITPAPATPQEYTIPTHFKVEVDDEVYEVR
CCHHHHHHHHHHHHHCHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCEEEEE
VEPLGGGVSISEASPKKPSAESVKGGVTSSMQGMVLSLKVKVEDTVREGDTVAVIEAMKM
EEECCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEEHHHHCCCCEEEEHHHHHH
ENAVHAPRSGIVKEIFVAEGDTVAPGDIILSIE
HHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MRVKITETVLRDAHQSLLATRMRTRDMLEVVEQLDQIGYFSLEMWGGATFDSCIRYLNED
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCC
PWQRLRDLKKNMDNTYAQMLLRGQNLVGYRHYSDDVVEKFVTKSYENGIDIFRVFDAVND
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VRNMELSIKVAKRLGAHVQGTVCYTISPVHTVEKYVGLAKQLEELECDSLCIKDMAGLLS
HHCCHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
PNEAAKIISAMKKEVSIPISLHCHCTSGMAPMSYMAACPAGVDVLDTALSPLAWGTSQPP
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
TETVVAALQGTPYDTGLDLGAFEEAVRYFKDLKEKYRGILDPISEQIDTNVLIYQIPGGM
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
LSNLVSQLKEQNALDKYGAVLQEMPKVREELGYPPLVTPTSQIVGTQAVLNVLMGERYKV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
IPKEVKDYVRGLYGRSPAPISPEIIGKIIGDEEPIHCRPADLLKPEYEKRKKEAEEMGIA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCC
NSEEDILTYILYPAIAPKFLKGEMEEEALAVITPAPATPQEYTIPTHFKVEVDDEVYEVR
CCHHHHHHHHHHHHHCHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCEEEEE
VEPLGGGVSISEASPKKPSAESVKGGVTSSMQGMVLSLKVKVEDTVREGDTVAVIEAMKM
EEECCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEEHHHHCCCCEEEEHHHHHH
ENAVHAPRSGIVKEIFVAEGDTVAPGDIILSIE
HHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]