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The map label for this gene is groEL [H]

Identifier: 21227900

GI number: 21227900

Start: 2146398

End: 2148008

Strand: Direct

Name: groEL [H]

Synonym: MM_1798

Alternate gene names: 21227900

Gene position: 2146398-2148008 (Clockwise)

Preceding gene: 21227899

Following gene: 21227901

Centisome position: 52.4

GC content: 48.36

Gene sequence:

>1611_bases
ATGGCTTCAAAGCAGATAATGTTTGATGAAAGTGCAAGAAAAGCACTCTTAAGCGGTGTGGATAAGGTCGCAAATACCGT
CAAGATCACCCTGGGGCCCAAAGGGCGTTATGTCGTTCTGGATAAAAGCACAAAACCCGTGGTCACAAATGACGGTGTCA
CCATTGCAAAAGAAATAGAGCTCCATGATAAGTTCGAAAACATGGGAGCCAAACTTGTTAAGGAAGTAGCCTCCAAAACC
CAGGACAACACCGGGGACGGGACAACGACAGCAACCCTTCTTGCCCAGAGCATGATCCGGGAAGGCCTGAAGAACATCAG
TGCCGGGGCAAACCCAATTGAAGTCAAGAAAGGGATTGAGATTGCCACGGAAAAGGTTGTGGGATACCTCAAAGGCAAGA
GTGTTGAGGTAAAAGGAAAGGATAAAATCATACAGGTAGCCACTATTTCCGCAAACAACGATGAAGAAATAGGCAACCTG
ATTGCCGACGCTATGGAAAGGGTCGGCTACAACGGGGTAATCACTGTTGAAGATTCAAAGACCATGGAGACCAGCCTTGA
TGTGGTCGAAGGTATGCAGTTCGACCGCGGGTTTGTTTCCCCGTACATGGCTCTTGACACCGAAAAGATGGTCTGCGAGT
TTGAAGACCCATATATCCTGATTACGGACAAAAAAATCAACAGCATGAAGCAGATCGTGCCTGTGCTTGAGAAAGTTGCT
TCTGAAGGGAGATCTCTCCTGATTATTGCTGAAGATGTCGACGGAGACGCCCAGGCTGCTTTGATTCTGAACATCATCCG
CGGAGCCCTCAGAGTTTGCGCTGTAAAAGCTCCGGGGTTCGGAAACGAGAGAAAAGAGATGCTTGAGGACATTGCTGTCC
TTACCGGTGGGCAGGTAATAAGCGAAGAAAAGGGCATGAAGCTTGAAGAATTCAGCGATTACATGCTTGGAAATGCCAGG
AAAGTCACGGTTGACAACCACAAAACCATTATCGTTGAAGGCAGGGGTGATAAGGCAGACATCGATGAAAGAGTCAGGGT
CATTGAAGCCCAGATCAATATTGCTGAAGCCGACTACAGAAAGACCGAACTTAAAAAGCGCATGGCAAAACTGGGCGGCG
GCGTTGCAGTAATTAAAGTTGGAGCCGCAACCGAAACCGAACTCAAGGAAAAGAAGATGAGAATCGATGACGCCCTGAAT
GCAACGAAAGCAGCAGTAGAGGAAGGTGTGGTCACAGGAGGAGGAATAAGTCTCTTCCGTGCGGCGGCGACCCTTGATTC
CCTTGACCTTGACGGCGACAGGCAGATTGGAGTAAAAATTGTCCAGAGAGCTATAGAAGACCCTGTACGCCAGATCGCTG
CAAATGCAGGAAGAGAAGGCGCTGAAGTGGTAGCAGCCATAAAAGCTGAATCCAGTGAGCATTTCGGGTACAACGCAAAA
ACGGATGTTTTTGAAGACCTCTTCGAAGCAGGAGTAATCGACCCGACAAAAGTCGTAAGAAGCGGGCTCCAGAATGCTGC
CTCAATCGCAGGCATGGTGCTCACAACAGAAGCTCTTGTAACAGACTTCGATGAAGAAAAGGATGAAAGGACAGCGGCAA
TCATTATCTGA

Upstream 100 bases:

>100_bases
ATTTAAGTAAATCAGGAATCCGATGAATGTCAGACAGTGATTTCGATAAATCATGAATTAATTCAATAAAAATATTTGAT
CTTAATTCAGGTGAATATAG

Downstream 100 bases:

>100_bases
TTGTATTTTTTCCTTTTTTTCCTCTCAATTTCTTCCTTCTTTATTCTTTCTCCTTTTTTCTCTATTTTTTTCTTTTATCT
TTACTCTTTCTCTATTTTTC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MASKQIMFDESARKALLSGVDKVANTVKITLGPKGRYVVLDKSTKPVVTNDGVTIAKEIELHDKFENMGAKLVKEVASKT
QDNTGDGTTTATLLAQSMIREGLKNISAGANPIEVKKGIEIATEKVVGYLKGKSVEVKGKDKIIQVATISANNDEEIGNL
IADAMERVGYNGVITVEDSKTMETSLDVVEGMQFDRGFVSPYMALDTEKMVCEFEDPYILITDKKINSMKQIVPVLEKVA
SEGRSLLIIAEDVDGDAQAALILNIIRGALRVCAVKAPGFGNERKEMLEDIAVLTGGQVISEEKGMKLEEFSDYMLGNAR
KVTVDNHKTIIVEGRGDKADIDERVRVIEAQINIAEADYRKTELKKRMAKLGGGVAVIKVGAATETELKEKKMRIDDALN
ATKAAVEEGVVTGGGISLFRAAATLDSLDLDGDRQIGVKIVQRAIEDPVRQIAANAGREGAEVVAAIKAESSEHFGYNAK
TDVFEDLFEAGVIDPTKVVRSGLQNAASIAGMVLTTEALVTDFDEEKDERTAAIII

Sequences:

>Translated_536_residues
MASKQIMFDESARKALLSGVDKVANTVKITLGPKGRYVVLDKSTKPVVTNDGVTIAKEIELHDKFENMGAKLVKEVASKT
QDNTGDGTTTATLLAQSMIREGLKNISAGANPIEVKKGIEIATEKVVGYLKGKSVEVKGKDKIIQVATISANNDEEIGNL
IADAMERVGYNGVITVEDSKTMETSLDVVEGMQFDRGFVSPYMALDTEKMVCEFEDPYILITDKKINSMKQIVPVLEKVA
SEGRSLLIIAEDVDGDAQAALILNIIRGALRVCAVKAPGFGNERKEMLEDIAVLTGGQVISEEKGMKLEEFSDYMLGNAR
KVTVDNHKTIIVEGRGDKADIDERVRVIEAQINIAEADYRKTELKKRMAKLGGGVAVIKVGAATETELKEKKMRIDDALN
ATKAAVEEGVVTGGGISLFRAAATLDSLDLDGDRQIGVKIVQRAIEDPVRQIAANAGREGAEVVAAIKAESSEHFGYNAK
TDVFEDLFEAGVIDPTKVVRSGLQNAASIAGMVLTTEALVTDFDEEKDERTAAIII
>Mature_535_residues
ASKQIMFDESARKALLSGVDKVANTVKITLGPKGRYVVLDKSTKPVVTNDGVTIAKEIELHDKFENMGAKLVKEVASKTQ
DNTGDGTTTATLLAQSMIREGLKNISAGANPIEVKKGIEIATEKVVGYLKGKSVEVKGKDKIIQVATISANNDEEIGNLI
ADAMERVGYNGVITVEDSKTMETSLDVVEGMQFDRGFVSPYMALDTEKMVCEFEDPYILITDKKINSMKQIVPVLEKVAS
EGRSLLIIAEDVDGDAQAALILNIIRGALRVCAVKAPGFGNERKEMLEDIAVLTGGQVISEEKGMKLEEFSDYMLGNARK
VTVDNHKTIIVEGRGDKADIDERVRVIEAQINIAEADYRKTELKKRMAKLGGGVAVIKVGAATETELKEKKMRIDDALNA
TKAAVEEGVVTGGGISLFRAAATLDSLDLDGDRQIGVKIVQRAIEDPVRQIAANAGREGAEVVAAIKAESSEHFGYNAKT
DVFEDLFEAGVIDPTKVVRSGLQNAASIAGMVLTTEALVTDFDEEKDERTAAIII

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=530, Percent_Identity=48.1132075471698, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI31542947, Length=530, Percent_Identity=48.1132075471698, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI24307939, Length=461, Percent_Identity=25.3796095444685, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790586, Length=527, Percent_Identity=54.4592030360531, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=44.3181818181818, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI193210679, Length=209, Percent_Identity=44.0191387559809, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI25144674, Length=265, Percent_Identity=26.0377358490566, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25147750, Length=557, Percent_Identity=24.7755834829443, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=48.4790874524715, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6322524, Length=357, Percent_Identity=22.96918767507, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=45.1612903225806, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=45.1612903225806, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45550936, Length=530, Percent_Identity=44.3396226415094, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550132, Length=530, Percent_Identity=44.3396226415094, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550935, Length=530, Percent_Identity=44.3396226415094, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864606, Length=528, Percent_Identity=41.0984848484849, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24584129, Length=521, Percent_Identity=35.1247600767754, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI19921262, Length=521, Percent_Identity=35.1247600767754, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI17647245, Length=530, Percent_Identity=24.3396226415094, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24652903, Length=504, Percent_Identity=24.8015873015873, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57767; Mature: 57636

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKQIMFDESARKALLSGVDKVANTVKITLGPKGRYVVLDKSTKPVVTNDGVTIAKEIE
CCCCCEEECHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCEEECCCEEEEEHHH
LHDKFENMGAKLVKEVASKTQDNTGDGTTTATLLAQSMIREGLKNISAGANPIEVKKGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHCCHH
IATEKVVGYLKGKSVEVKGKDKIIQVATISANNDEEIGNLIADAMERVGYNGVITVEDSK
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
TMETSLDVVEGMQFDRGFVSPYMALDTEKMVCEFEDPYILITDKKINSMKQIVPVLEKVA
HHHHHHHHHHCCCCCCCCCCCHHHCCCHHHEEEECCCEEEEECCHHHHHHHHHHHHHHHH
SEGRSLLIIAEDVDGDAQAALILNIIRGALRVCAVKAPGFGNERKEMLEDIAVLTGGQVI
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCEE
SEEKGMKLEEFSDYMLGNARKVTVDNHKTIIVEGRGDKADIDERVRVIEAQINIAEADYR
CCCCCCCHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHEEEHHHHHH
KTELKKRMAKLGGGVAVIKVGAATETELKEKKMRIDDALNATKAAVEEGVVTGGGISLFR
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AAATLDSLDLDGDRQIGVKIVQRAIEDPVRQIAANAGREGAEVVAAIKAESSEHFGYNAK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCCCCCCC
TDVFEDLFEAGVIDPTKVVRSGLQNAASIAGMVLTTEALVTDFDEEKDERTAAIII
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure 
ASKQIMFDESARKALLSGVDKVANTVKITLGPKGRYVVLDKSTKPVVTNDGVTIAKEIE
CCCCEEECHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCEEECCCEEEEEHHH
LHDKFENMGAKLVKEVASKTQDNTGDGTTTATLLAQSMIREGLKNISAGANPIEVKKGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHCCHH
IATEKVVGYLKGKSVEVKGKDKIIQVATISANNDEEIGNLIADAMERVGYNGVITVEDSK
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
TMETSLDVVEGMQFDRGFVSPYMALDTEKMVCEFEDPYILITDKKINSMKQIVPVLEKVA
HHHHHHHHHHCCCCCCCCCCCHHHCCCHHHEEEECCCEEEEECCHHHHHHHHHHHHHHHH
SEGRSLLIIAEDVDGDAQAALILNIIRGALRVCAVKAPGFGNERKEMLEDIAVLTGGQVI
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCEE
SEEKGMKLEEFSDYMLGNARKVTVDNHKTIIVEGRGDKADIDERVRVIEAQINIAEADYR
CCCCCCCHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHEEEHHHHHH
KTELKKRMAKLGGGVAVIKVGAATETELKEKKMRIDDALNATKAAVEEGVVTGGGISLFR
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AAATLDSLDLDGDRQIGVKIVQRAIEDPVRQIAANAGREGAEVVAAIKAESSEHFGYNAK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCCCCCCC
TDVFEDLFEAGVIDPTKVVRSGLQNAASIAGMVLTTEALVTDFDEEKDERTAAIII
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]