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The map label for this gene is ppdK [H]

Identifier: 21227872

GI number: 21227872

Start: 2111826

End: 2114480

Strand: Reverse

Name: ppdK [H]

Synonym: MM_1770

Alternate gene names: 21227872

Gene position: 2114480-2111826 (Counterclockwise)

Preceding gene: 21227876

Following gene: 21227870

Centisome position: 51.62

GC content: 47.98

Gene sequence:

>2655_bases
TTGTCCAAGTTCGTATATTTTTTTGGAAAAGATGTAACTGATGGAAAAAGTAGTATGAAAGACCTGCTTGGAGGTAAGGG
TGCAAACCTTGCCGAGATGGCAAATCTTGGAGTTCCCGTACCACCAGGTTTTACAATCTCAACTGAAGTCTGTGTTCTTT
ATTTAAAGGAAGGAAAATATCCAGAAGAGGTTCTCAGACAGGTAGAAGAGGCGATCGATAAATTAGAAAAATTAAACGGC
AAAAAGCTGGGAGATCCCGAAGATCCGCTGCTTGTTTCAGTAAGATCCGGAGCACGGGTGTCCATGCCAGGAATGATGGA
CACTGTTCTGAACCTTGGACTTACGGATAAATCCGTAATCGGGCTCGCAAATAAAGTCAATGACGAAAGATTCGCTTACG
ACTGCTACCGAAGATTCATCGCCATGTTTGGGGACGTGGTGCTCGGGGTTGAATTCAGAAAATTCGATTCCTTAATCGAC
GACAAGAAAAAGGAGCTGGGGGTCAAGTCCGACACAGACCTCGATTCAAAAGCTTTACAGGAATTGGCAGAGAGATTCAA
GGAAGTAATCAAACTCGAAAAAGGATTTGAATTCCCACAGGATCCTAAAGTTCAGCTTCAGATGGCAATCAACGCTGTTT
TTGATTCATGGAATAACCAGAGAGCCATCACCTACAGGAAGCTTAACGCTATTGATGACAGCTGGGGCACAGCCGTCAAT
GTACAGACAATGGTCTACGGTAACAGAGGGAACACCTCAGGTACAGGCGTTGCTTTTACCAGAAACCCGTCAACAGGCGA
AAAGAAGTTTTTCGGAGAATACCTCATCAATGCACAGGGCGAAGATGTGGTTGCAGGAATAAGGACCCCCGACTATATTG
ATACTCTCGGAAACAAAATTCCTGAAGCCTATGCCCAGCTTGTGGACATCTGCCAGAAACTTGAAGCCCATTTCAAGGAC
ATGCAGGACATAGAGTTCACAATCCAGGAAGGAAAACTCTATATGCTGCAGACCAGGACCGGAAAGCGCACAGCTGCTGC
CGCAGTAAAGATTGCGACCGAGATGGTGGCAGAAGGACTCATAAACAAAGAAACTGCTGTCAAAAGAGTTAATGCTGAAC
ATATTGACCTTCTCCTGCACCCGAGAATTGACCCGAAAGCAAAACTCGAAGTAATAGCAAAAGGTCTCCCTGCATCACCA
GGAGCCGCTGTCGGAAAGGTGGTCTTTACCGCTGAAGATGCAGAAGAGATGGCAGAGAACGGTGAGAAAACAATTCTTGT
CCGTACCGAGACCTCACCTGAAGACATAGGGGGAATGGCAGCTGCACAGGGAGTTCTCACCGTGCGCGGAGGGATGACCT
CTCATGCTGCAGTCGTAGGTAGAGGCATGGGTAAGCCGTGTGTTGTTGGTTGCGGAGAAATCTCAATCGACATGAAGAAC
GCCCTCTTTATGGTAAACGGTTTCACAATTAAAGAGCACGACTACATCACAATTGACGGAAGCGTTGGAAGCGTCATTAT
CGGAAAAGTAGACCTTATAGACGCAGAAATAAATGAGGACCTGAAAAAGCTCCTTATATGGGCTGACAAGATAAGGACTC
TGGGAGTAAGGACAAACGCTGACAACCCGGCTGACGCAGCCCTCGCCCGCGAGCTCGGAGCAGAAGGTATCGGGCTTTGC
AGGACAGAGCACATGTTCTTTGGCGAAGACAGGATCCCTGCAGTAAGGGAGATGATCATGGCCGAAGATGAAAAATCAAG
GAAGAAAGCTCTCAAGAAACTGCTCCCAATGCAGAAAGAAGACTTCCTTGGAATCTTCCGCAGTATGGAAGGCCTGCCCG
TAACCATCAGACTCCTTGACCCGCCTCTCCACGAGTTCCTCCCGGACAAAGAAGAACTTGACACAAAACTTAGAGAACTT
GAAACATCGGGAGACTGCGGAAAGATCGATGGTGTAAAGAAAGTAATCCAGCGCGTAGTTTCACTTAAGGAACTCAACCC
AATGCTCGGCCACAGGGGATGCAGGCTCGGAATTACCTATCCTGAAATATACAACATGCAGGTGCGTGCAATCATGGAGG
CAGCCTGTGAACTTACTGCCGAGGGAATGGAAGTAGTTCCTGAAATCATGATTCCGCTTGTGGGCATTGTAAAAGAGCTC
TCCCTCTCCAGAGAGGAAGTATGCAAGACTGCAGAAGCCGTAATGGCTGAGAAGGGCATGAAGATCGACTACAAGGTAGG
GACCATGATCGAACTGCCCAGGGCTGCAATCGTCGCAGACCAGATCGCAAAGGAAGCTGACTTCTTCTCCTTCGGGACAA
ACGACCTGACACAGACAACCTTCGGGTTCAGCAGGGACGATGTGTCCAAGTTCGTGCCCATATACCAGAAGGCAGGCATC
CTTGAACACGACCCCTTTGCAGTCCTTGACCAGGAAGGCGTTGGCGAGATCATGAAGATAGGTATCTCAAAAGGCCGCTC
CGTTAAACCCAAACTCAAGATGGGAATCTGTGGAGAACACGGAGGAGAGCCGAAATCCATTGCTTTTGCTCACGAAATAG
GTATTGACTATGTGAGCTGCTCCCCTTACAGAGTGCCCATTGCAAGGCTTGTGGCTGCTCAGAGCACAATGGAAATGAGA
GACGCAAAAAAATAA

Upstream 100 bases:

>100_bases
TAATAATTAAATAGCCATTGTTGGCAACAACCGGACCTATGGTCTGGAAATAATTGACTTTAGCTCCGATTAATGATCTC
GAATCCAGGGAGGAGAATCA

Downstream 100 bases:

>100_bases
AGAAACTGAAATTTGAATTCCGGCAGGACTAAATCCTGCCTTCAACCTCTTTTTTAAAAAAGTTACTTTCTTGAGCGGAG
CAAATCTATCTGTCGATCCG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 884; Mature: 883

Protein sequence:

>884_residues
MSKFVYFFGKDVTDGKSSMKDLLGGKGANLAEMANLGVPVPPGFTISTEVCVLYLKEGKYPEEVLRQVEEAIDKLEKLNG
KKLGDPEDPLLVSVRSGARVSMPGMMDTVLNLGLTDKSVIGLANKVNDERFAYDCYRRFIAMFGDVVLGVEFRKFDSLID
DKKKELGVKSDTDLDSKALQELAERFKEVIKLEKGFEFPQDPKVQLQMAINAVFDSWNNQRAITYRKLNAIDDSWGTAVN
VQTMVYGNRGNTSGTGVAFTRNPSTGEKKFFGEYLINAQGEDVVAGIRTPDYIDTLGNKIPEAYAQLVDICQKLEAHFKD
MQDIEFTIQEGKLYMLQTRTGKRTAAAAVKIATEMVAEGLINKETAVKRVNAEHIDLLLHPRIDPKAKLEVIAKGLPASP
GAAVGKVVFTAEDAEEMAENGEKTILVRTETSPEDIGGMAAAQGVLTVRGGMTSHAAVVGRGMGKPCVVGCGEISIDMKN
ALFMVNGFTIKEHDYITIDGSVGSVIIGKVDLIDAEINEDLKKLLIWADKIRTLGVRTNADNPADAALARELGAEGIGLC
RTEHMFFGEDRIPAVREMIMAEDEKSRKKALKKLLPMQKEDFLGIFRSMEGLPVTIRLLDPPLHEFLPDKEELDTKLREL
ETSGDCGKIDGVKKVIQRVVSLKELNPMLGHRGCRLGITYPEIYNMQVRAIMEAACELTAEGMEVVPEIMIPLVGIVKEL
SLSREEVCKTAEAVMAEKGMKIDYKVGTMIELPRAAIVADQIAKEADFFSFGTNDLTQTTFGFSRDDVSKFVPIYQKAGI
LEHDPFAVLDQEGVGEIMKIGISKGRSVKPKLKMGICGEHGGEPKSIAFAHEIGIDYVSCSPYRVPIARLVAAQSTMEMR
DAKK

Sequences:

>Translated_884_residues
MSKFVYFFGKDVTDGKSSMKDLLGGKGANLAEMANLGVPVPPGFTISTEVCVLYLKEGKYPEEVLRQVEEAIDKLEKLNG
KKLGDPEDPLLVSVRSGARVSMPGMMDTVLNLGLTDKSVIGLANKVNDERFAYDCYRRFIAMFGDVVLGVEFRKFDSLID
DKKKELGVKSDTDLDSKALQELAERFKEVIKLEKGFEFPQDPKVQLQMAINAVFDSWNNQRAITYRKLNAIDDSWGTAVN
VQTMVYGNRGNTSGTGVAFTRNPSTGEKKFFGEYLINAQGEDVVAGIRTPDYIDTLGNKIPEAYAQLVDICQKLEAHFKD
MQDIEFTIQEGKLYMLQTRTGKRTAAAAVKIATEMVAEGLINKETAVKRVNAEHIDLLLHPRIDPKAKLEVIAKGLPASP
GAAVGKVVFTAEDAEEMAENGEKTILVRTETSPEDIGGMAAAQGVLTVRGGMTSHAAVVGRGMGKPCVVGCGEISIDMKN
ALFMVNGFTIKEHDYITIDGSVGSVIIGKVDLIDAEINEDLKKLLIWADKIRTLGVRTNADNPADAALARELGAEGIGLC
RTEHMFFGEDRIPAVREMIMAEDEKSRKKALKKLLPMQKEDFLGIFRSMEGLPVTIRLLDPPLHEFLPDKEELDTKLREL
ETSGDCGKIDGVKKVIQRVVSLKELNPMLGHRGCRLGITYPEIYNMQVRAIMEAACELTAEGMEVVPEIMIPLVGIVKEL
SLSREEVCKTAEAVMAEKGMKIDYKVGTMIELPRAAIVADQIAKEADFFSFGTNDLTQTTFGFSRDDVSKFVPIYQKAGI
LEHDPFAVLDQEGVGEIMKIGISKGRSVKPKLKMGICGEHGGEPKSIAFAHEIGIDYVSCSPYRVPIARLVAAQSTMEMR
DAKK
>Mature_883_residues
SKFVYFFGKDVTDGKSSMKDLLGGKGANLAEMANLGVPVPPGFTISTEVCVLYLKEGKYPEEVLRQVEEAIDKLEKLNGK
KLGDPEDPLLVSVRSGARVSMPGMMDTVLNLGLTDKSVIGLANKVNDERFAYDCYRRFIAMFGDVVLGVEFRKFDSLIDD
KKKELGVKSDTDLDSKALQELAERFKEVIKLEKGFEFPQDPKVQLQMAINAVFDSWNNQRAITYRKLNAIDDSWGTAVNV
QTMVYGNRGNTSGTGVAFTRNPSTGEKKFFGEYLINAQGEDVVAGIRTPDYIDTLGNKIPEAYAQLVDICQKLEAHFKDM
QDIEFTIQEGKLYMLQTRTGKRTAAAAVKIATEMVAEGLINKETAVKRVNAEHIDLLLHPRIDPKAKLEVIAKGLPASPG
AAVGKVVFTAEDAEEMAENGEKTILVRTETSPEDIGGMAAAQGVLTVRGGMTSHAAVVGRGMGKPCVVGCGEISIDMKNA
LFMVNGFTIKEHDYITIDGSVGSVIIGKVDLIDAEINEDLKKLLIWADKIRTLGVRTNADNPADAALARELGAEGIGLCR
TEHMFFGEDRIPAVREMIMAEDEKSRKKALKKLLPMQKEDFLGIFRSMEGLPVTIRLLDPPLHEFLPDKEELDTKLRELE
TSGDCGKIDGVKKVIQRVVSLKELNPMLGHRGCRLGITYPEIYNMQVRAIMEAACELTAEGMEVVPEIMIPLVGIVKELS
LSREEVCKTAEAVMAEKGMKIDYKVGTMIELPRAAIVADQIAKEADFFSFGTNDLTQTTFGFSRDDVSKFVPIYQKAGIL
EHDPFAVLDQEGVGEIMKIGISKGRSVKPKLKMGICGEHGGEPKSIAFAHEIGIDYVSCSPYRVPIARLVAAQSTMEMRD
AKK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=481, Percent_Identity=24.948024948025, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI48994992, Length=464, Percent_Identity=23.9224137931034, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789193, Length=473, Percent_Identity=23.6786469344609, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788726, Length=317, Percent_Identity=25.8675078864353, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97109; Mature: 96977

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFVYFFGKDVTDGKSSMKDLLGGKGANLAEMANLGVPVPPGFTISTEVCVLYLKEGKY
CCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEECHHEEEEEEECCCC
PEEVLRQVEEAIDKLEKLNGKKLGDPEDPLLVSVRSGARVSMPGMMDTVLNLGLTDKSVI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCHHHH
GLANKVNDERFAYDCYRRFIAMFGDVVLGVEFRKFDSLIDDKKKELGVKSDTDLDSKALQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ELAERFKEVIKLEKGFEFPQDPKVQLQMAINAVFDSWNNQRAITYRKLNAIDDSWGTAVN
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
VQTMVYGNRGNTSGTGVAFTRNPSTGEKKFFGEYLINAQGEDVVAGIRTPDYIDTLGNKI
EEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHH
PEAYAQLVDICQKLEAHFKDMQDIEFTIQEGKLYMLQTRTGKRTAAAAVKIATEMVAEGL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
INKETAVKRVNAEHIDLLLHPRIDPKAKLEVIAKGLPASPGAAVGKVVFTAEDAEEMAEN
CCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHCC
GEKTILVRTETSPEDIGGMAAAQGVLTVRGGMTSHAAVVGRGMGKPCVVGCGEISIDMKN
CCEEEEEEECCCHHHHCCHHHHCCEEEEECCCCHHHHHHHCCCCCCEEEECCEEEEECCC
ALFMVNGFTIKEHDYITIDGSVGSVIIGKVDLIDAEINEDLKKLLIWADKIRTLGVRTNA
EEEEEECEEEECCCEEEECCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCC
DNPADAALARELGAEGIGLCRTEHMFFGEDRIPAVREMIMAEDEKSRKKALKKLLPMQKE
CCCHHHHHHHHHCCCCCCEEEECCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHH
DFLGIFRSMEGLPVTIRLLDPPLHEFLPDKEELDTKLRELETSGDCGKIDGVKKVIQRVV
HHHHHHHCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SLKELNPMLGHRGCRLGITYPEIYNMQVRAIMEAACELTAEGMEVVPEIMIPLVGIVKEL
HHHHCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSREEVCKTAEAVMAEKGMKIDYKVGTMIELPRAAIVADQIAKEADFFSFGTNDLTQTT
CCCHHHHHHHHHHHHHHCCCEEEEECCCEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
FGFSRDDVSKFVPIYQKAGILEHDPFAVLDQEGVGEIMKIGISKGRSVKPKLKMGICGEH
CCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCEEEEECCCC
GGEPKSIAFAHEIGIDYVSCSPYRVPIARLVAAQSTMEMRDAKK
CCCCCCEEEHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKFVYFFGKDVTDGKSSMKDLLGGKGANLAEMANLGVPVPPGFTISTEVCVLYLKEGKY
CCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEECHHEEEEEEECCCC
PEEVLRQVEEAIDKLEKLNGKKLGDPEDPLLVSVRSGARVSMPGMMDTVLNLGLTDKSVI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCHHHH
GLANKVNDERFAYDCYRRFIAMFGDVVLGVEFRKFDSLIDDKKKELGVKSDTDLDSKALQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ELAERFKEVIKLEKGFEFPQDPKVQLQMAINAVFDSWNNQRAITYRKLNAIDDSWGTAVN
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
VQTMVYGNRGNTSGTGVAFTRNPSTGEKKFFGEYLINAQGEDVVAGIRTPDYIDTLGNKI
EEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHH
PEAYAQLVDICQKLEAHFKDMQDIEFTIQEGKLYMLQTRTGKRTAAAAVKIATEMVAEGL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
INKETAVKRVNAEHIDLLLHPRIDPKAKLEVIAKGLPASPGAAVGKVVFTAEDAEEMAEN
CCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHCC
GEKTILVRTETSPEDIGGMAAAQGVLTVRGGMTSHAAVVGRGMGKPCVVGCGEISIDMKN
CCEEEEEEECCCHHHHCCHHHHCCEEEEECCCCHHHHHHHCCCCCCEEEECCEEEEECCC
ALFMVNGFTIKEHDYITIDGSVGSVIIGKVDLIDAEINEDLKKLLIWADKIRTLGVRTNA
EEEEEECEEEECCCEEEECCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCC
DNPADAALARELGAEGIGLCRTEHMFFGEDRIPAVREMIMAEDEKSRKKALKKLLPMQKE
CCCHHHHHHHHHCCCCCCEEEECCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHH
DFLGIFRSMEGLPVTIRLLDPPLHEFLPDKEELDTKLRELETSGDCGKIDGVKKVIQRVV
HHHHHHHCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SLKELNPMLGHRGCRLGITYPEIYNMQVRAIMEAACELTAEGMEVVPEIMIPLVGIVKEL
HHHHCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSREEVCKTAEAVMAEKGMKIDYKVGTMIELPRAAIVADQIAKEADFFSFGTNDLTQTT
CCCHHHHHHHHHHHHHHCCCEEEEECCCEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
FGFSRDDVSKFVPIYQKAGILEHDPFAVLDQEGVGEIMKIGISKGRSVKPKLKMGICGEH
CCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCEEEEECCCC
GGEPKSIAFAHEIGIDYVSCSPYRVPIARLVAAQSTMEMRDAKK
CCCCCCEEEHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]