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The map label for this gene is hemB [H]

Identifier: 21227844

GI number: 21227844

Start: 2083290

End: 2084264

Strand: Direct

Name: hemB [H]

Synonym: MM_1742

Alternate gene names: 21227844

Gene position: 2083290-2084264 (Clockwise)

Preceding gene: 21227843

Following gene: 21227845

Centisome position: 50.86

GC content: 49.13

Gene sequence:

>975_bases
ATGTTTCCAGAAATCAGGTTGAGAAGGTTGAGAAAAGGGAAGATCAGGGACCTTGTGCGTGAGACCACCTTATCCGTGGA
CGACCTGGTTATGCCCATTTTTGTGAATGAGAATATTGATTCCCCTGTTGAGATCTCTTCCATGCCGGGAATATACAATT
TTCCCCTTTCTGAGGTTGCAAAAGAGGCAAAAGAGATTGCAGACCTCGGGATTCCGGCAGTGATCCTTTTCGGAGTCCCT
GCATTCAAAGATGCCGAGGGCAGCACTTCCTGCGGGGAAAACGACGTGGTTCAGGAAGCTGTTAGGAGAATCAAAGCCGA
ACTTGGAAACAGGCTTGTTGTGATCACGGATATCTGCATGTGCGAATTCACAAGCCACGGCCACTGCGGAATAGTCGACT
TTGAAACTCAGGAAGTCCTGAATGACCCCACCCTGGAAGTCCTGGGAAAAATTGCTGTCAGCCACGCAAGAGCCGGAGCA
GATATGGTAGCTCCTTCAGGAATGATGGACGGGATGGTAGGTGCAATCCGTATGGCTCTTGACGCAAGCGGGTTTGAGAA
TATTCCTATTATGTCCTATGCCGCAAAATATCATTCCTGTTTCTACGGTCCTTTCAGAGAAGCTGCAGAATCCGGTTATT
CCTTCGGGGACCGTTCAACCTATCAGATGGACCCTGCAAACAGTGACGAAGCTCTCAGGGAAGTAGCCCTTGATGTGGCA
GAAGGTGCGGATATTCTTATGGTGAAGCCCGGGCTTCCGTATCTTGATATCGTGTACAGGGTCAAGAGCGAGTTCGGCAT
GCCAACAGCCGCATACAATGTGAGCGGGGAATATTCCATGATCAAAGCCGCAGCAGCCAGCGGCTGGATTGATGAGAAAA
AGGCTATTTACGAGTCCCTTATCTCCATAAAAAGGGCAGGTGCGGATTTCATCATCACCTACTTTGCAAAGGATGCTGCT
AGGATGCTGAAATAA

Upstream 100 bases:

>100_bases
ATAAATAATCAAATAAATTTACAGATAAAACAAATCAATTCTCAATAAAAACAATCAAATCAATTCACAGGTAAGCATTT
CATATTTCAGGAGTGATCAC

Downstream 100 bases:

>100_bases
CAGCTTCTTCAGGCATTATTTTTTAAGGGGCTTCAGTCTGTTTTTTCCTTTCCTTTTACTTTTCATTTTTAAGCCTGCCT
GTTAATTCTGCCTGTATAAG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MFPEIRLRRLRKGKIRDLVRETTLSVDDLVMPIFVNENIDSPVEISSMPGIYNFPLSEVAKEAKEIADLGIPAVILFGVP
AFKDAEGSTSCGENDVVQEAVRRIKAELGNRLVVITDICMCEFTSHGHCGIVDFETQEVLNDPTLEVLGKIAVSHARAGA
DMVAPSGMMDGMVGAIRMALDASGFENIPIMSYAAKYHSCFYGPFREAAESGYSFGDRSTYQMDPANSDEALREVALDVA
EGADILMVKPGLPYLDIVYRVKSEFGMPTAAYNVSGEYSMIKAAAASGWIDEKKAIYESLISIKRAGADFIITYFAKDAA
RMLK

Sequences:

>Translated_324_residues
MFPEIRLRRLRKGKIRDLVRETTLSVDDLVMPIFVNENIDSPVEISSMPGIYNFPLSEVAKEAKEIADLGIPAVILFGVP
AFKDAEGSTSCGENDVVQEAVRRIKAELGNRLVVITDICMCEFTSHGHCGIVDFETQEVLNDPTLEVLGKIAVSHARAGA
DMVAPSGMMDGMVGAIRMALDASGFENIPIMSYAAKYHSCFYGPFREAAESGYSFGDRSTYQMDPANSDEALREVALDVA
EGADILMVKPGLPYLDIVYRVKSEFGMPTAAYNVSGEYSMIKAAAASGWIDEKKAIYESLISIKRAGADFIITYFAKDAA
RMLK
>Mature_324_residues
MFPEIRLRRLRKGKIRDLVRETTLSVDDLVMPIFVNENIDSPVEISSMPGIYNFPLSEVAKEAKEIADLGIPAVILFGVP
AFKDAEGSTSCGENDVVQEAVRRIKAELGNRLVVITDICMCEFTSHGHCGIVDFETQEVLNDPTLEVLGKIAVSHARAGA
DMVAPSGMMDGMVGAIRMALDASGFENIPIMSYAAKYHSCFYGPFREAAESGYSFGDRSTYQMDPANSDEALREVALDVA
EGADILMVKPGLPYLDIVYRVKSEFGMPTAAYNVSGEYSMIKAAAASGWIDEKKAIYESLISIKRAGADFIITYFAKDAA
RMLK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=324, Percent_Identity=42.2839506172839, Blast_Score=251, Evalue=4e-67,
Organism=Escherichia coli, GI87081728, Length=316, Percent_Identity=52.2151898734177, Blast_Score=295, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6321398, Length=309, Percent_Identity=38.8349514563107, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=39.873417721519, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35399; Mature: 35399

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPEIRLRRLRKGKIRDLVRETTLSVDDLVMPIFVNENIDSPVEISSMPGIYNFPLSEVA
CCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCCEECCCCCCCCCCHHHHH
KEAKEIADLGIPAVILFGVPAFKDAEGSTSCGENDVVQEAVRRIKAELGNRLVVITDICM
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHH
CEFTSHGHCGIVDFETQEVLNDPTLEVLGKIAVSHARAGADMVAPSGMMDGMVGAIRMAL
HHCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
DASGFENIPIMSYAAKYHSCFYGPFREAAESGYSFGDRSTYQMDPANSDEALREVALDVA
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
EGADILMVKPGLPYLDIVYRVKSEFGMPTAAYNVSGEYSMIKAAAASGWIDEKKAIYESL
CCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
ISIKRAGADFIITYFAKDAARMLK
HHHHHCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MFPEIRLRRLRKGKIRDLVRETTLSVDDLVMPIFVNENIDSPVEISSMPGIYNFPLSEVA
CCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCCEECCCCCCCCCCHHHHH
KEAKEIADLGIPAVILFGVPAFKDAEGSTSCGENDVVQEAVRRIKAELGNRLVVITDICM
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHH
CEFTSHGHCGIVDFETQEVLNDPTLEVLGKIAVSHARAGADMVAPSGMMDGMVGAIRMAL
HHCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
DASGFENIPIMSYAAKYHSCFYGPFREAAESGYSFGDRSTYQMDPANSDEALREVALDVA
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
EGADILMVKPGLPYLDIVYRVKSEFGMPTAAYNVSGEYSMIKAAAASGWIDEKKAIYESL
CCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
ISIKRAGADFIITYFAKDAARMLK
HHHHHCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]