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The map label for this gene is pcm

Identifier: 21227808

GI number: 21227808

Start: 2049308

End: 2050039

Strand: Reverse

Name: pcm

Synonym: MM_1706

Alternate gene names: 21227808

Gene position: 2050039-2049308 (Counterclockwise)

Preceding gene: 21227809

Following gene: 21227807

Centisome position: 50.05

GC content: 45.36

Gene sequence:

>732_bases
TTGCCAGAAAGAAGGGGGATAGTTCTGGTCAACGCAGTTTCAGAAAAGGACCTTGGTGAGGATAAAAGAAAAAGAGAAAA
AGAAAAAGAATGTGAGAAGCTCCGCAAGCTCCTTGTAGAAGGTCTTGAAGGTTTCGTAATAGACGAAAAAGTGCTCCAGG
CCATGCTCCGTGTCCCAAGGCACAGGTTTGTTCCGGAATATGAGCAAAGGGCAGCTTATGTAGACATGCCTCTTGAAATA
GGCCATGGGCAAACAATATCTGCCCCCCACATGGTTGCTATGATGTGTGAAATCCTGGAGCTGGCTGAAGGGCATAAAGT
TCTGGAAATTGGAGCAGGTTCAGGATATAATGCTGCAGTGATGTCAGAGCTTGTAGGAAAAACCGGGCATATTTACACTG
TGGAGCGGGTAGAACCTCTTGCAAATTTTGCAAAGAAGAACCTGAAAGAAGCAGGGTATAAAAATGTTACAGTTTTACTT
GAGAATGGGTCAATGGGTTATCCCGGATATGCCCCCTATGACAGGATAGCTGTAACATGCGCAGCTCCCAATATCCCTGA
AACTCTCCTGGAACAATTGAAGCCCGGGGGTATAATGGTAATCCCTGTGGGGAGCTATTCCCAGGAGCTAATCAGGGTTA
AAAAAGACAGCACAGGCAAGATATACAGGAAGAAAAAAGGCGACGTTATCTTTGTGCCTATGATTGGCAAACACGGCTTT
CGAAGGATTTGA

Upstream 100 bases:

>100_bases
GGAAATACCCGTCTTTTCATTACCAAGATAAAGATTAGAGACGGGAATTTCAGATCCAGAAAAGGTGACGCTGTACTTGC
AAAATATGTGAAAAGGATTT

Downstream 100 bases:

>100_bases
GAGAGAGAGAACCGGAATTAAAAAGTATGAAATAAAACTCAAAGATTCTTCTTTAATAATCTGAAAAGAATTAATAAATT
ATTAATAAGTGTGAAATTAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPERRGIVLVNAVSEKDLGEDKRKREKEKECEKLRKLLVEGLEGFVIDEKVLQAMLRVPRHRFVPEYEQRAAYVDMPLEI
GHGQTISAPHMVAMMCEILELAEGHKVLEIGAGSGYNAAVMSELVGKTGHIYTVERVEPLANFAKKNLKEAGYKNVTVLL
ENGSMGYPGYAPYDRIAVTCAAPNIPETLLEQLKPGGIMVIPVGSYSQELIRVKKDSTGKIYRKKKGDVIFVPMIGKHGF
RRI

Sequences:

>Translated_243_residues
MPERRGIVLVNAVSEKDLGEDKRKREKEKECEKLRKLLVEGLEGFVIDEKVLQAMLRVPRHRFVPEYEQRAAYVDMPLEI
GHGQTISAPHMVAMMCEILELAEGHKVLEIGAGSGYNAAVMSELVGKTGHIYTVERVEPLANFAKKNLKEAGYKNVTVLL
ENGSMGYPGYAPYDRIAVTCAAPNIPETLLEQLKPGGIMVIPVGSYSQELIRVKKDSTGKIYRKKKGDVIFVPMIGKHGF
RRI
>Mature_242_residues
PERRGIVLVNAVSEKDLGEDKRKREKEKECEKLRKLLVEGLEGFVIDEKVLQAMLRVPRHRFVPEYEQRAAYVDMPLEIG
HGQTISAPHMVAMMCEILELAEGHKVLEIGAGSGYNAAVMSELVGKTGHIYTVERVEPLANFAKKNLKEAGYKNVTVLLE
NGSMGYPGYAPYDRIAVTCAAPNIPETLLEQLKPGGIMVIPVGSYSQELIRVKKDSTGKIYRKKKGDVIFVPMIGKHGFR
RI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=204, Percent_Identity=36.2745098039216, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1789100, Length=202, Percent_Identity=42.0792079207921, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71983477, Length=197, Percent_Identity=34.010152284264, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI193207222, Length=195, Percent_Identity=31.7948717948718, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17981723, Length=206, Percent_Identity=33.9805825242718, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_METMA (Q8PW90)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633730.1
- ProteinModelPortal:   Q8PW90
- SMR:   Q8PW90
- GeneID:   1480048
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1706
- NMPDR:   fig|192952.1.peg.1706
- HOGENOM:   HBG699907
- OMA:   LCPVRFV
- ProtClustDB:   PRK13942
- BioCyc:   MMAZ192952:MM1706-MONOMER
- BRENDA:   2.1.1.77
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 27120; Mature: 26989

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 87-87

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERRGIVLVNAVSEKDLGEDKRKREKEKECEKLRKLLVEGLEGFVIDEKVLQAMLRVPR
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHCCH
HRFVPEYEQRAAYVDMPLEIGHGQTISAPHMVAMMCEILELAEGHKVLEIGAGSGYNAAV
HCCCCCHHHCCEEECCCEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
MSELVGKTGHIYTVERVEPLANFAKKNLKEAGYKNVTVLLENGSMGYPGYAPYDRIAVTC
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
AAPNIPETLLEQLKPGGIMVIPVGSYSQELIRVKKDSTGKIYRKKKGDVIFVPMIGKHGF
CCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCCC
RRI
CCC
>Mature Secondary Structure 
PERRGIVLVNAVSEKDLGEDKRKREKEKECEKLRKLLVEGLEGFVIDEKVLQAMLRVPR
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHCCH
HRFVPEYEQRAAYVDMPLEIGHGQTISAPHMVAMMCEILELAEGHKVLEIGAGSGYNAAV
HCCCCCHHHCCEEECCCEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
MSELVGKTGHIYTVERVEPLANFAKKNLKEAGYKNVTVLLENGSMGYPGYAPYDRIAVTC
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
AAPNIPETLLEQLKPGGIMVIPVGSYSQELIRVKKDSTGKIYRKKKGDVIFVPMIGKHGF
CCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCCC
RRI
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824