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The map label for this gene is nfsA [C]

Identifier: 21227686

GI number: 21227686

Start: 1884259

End: 1884783

Strand: Reverse

Name: nfsA [C]

Synonym: MM_1584

Alternate gene names: 21227686

Gene position: 1884783-1884259 (Counterclockwise)

Preceding gene: 21227687

Following gene: 21227685

Centisome position: 46.01

GC content: 50.86

Gene sequence:

>525_bases
ATGGAAACTCTTGAAGCAATCCACACTCGCCGAAGCATCCGAAAGTACACTGACAGACCAGTTCCGAGAGAGCTTGTTAC
CGAACTGCTCAGGGCAGCAATGAGTGCCCCCTCTGCGGTTAATGCCCAGCCCTGGGTCTTCATTGTAATCGATGACAGAA
AAATTCTGGACGAGATCCCAACATTCAGCCCTTATGCCGGCATGTGCAGGGAAGCTCCTCTGGCAATTCTGGTCTGTGGG
GATACTACCCAGGAAAAAGCACCCGGGTACTGGGTACAGGACTGCTCGGCAGCCATTCAGAACCTTCTTCTTGCAGCTCA
TGATGCAGGGCTGGGAGCGGTCTGGACAGGAATTTTTCCCATGACAGACAGAGTTCAAGGCTTCAGGAAAGCATTTGGAC
TGCCTGGCCATGTATTTCCTCTCGGGCTTGTGCCTGTTGGTTATCCTGCAGAAAAACCCTCACCTCAGGACAGGTACAGG
GAAGAGAAAGTCTACAATAACAGATACGGTCAAAAAAGAGAATAA

Upstream 100 bases:

>100_bases
GGAAAGAGAATAAAAACGGGATAATAAGACGGGGCGAGAGAAAACTGGAAATAGGTTTCCTCGTCCAAGTCTTTCATCAA
CGAACAGGAGGAGTCTGAAT

Downstream 100 bases:

>100_bases
TTCTGCAAATACTGGCAGATCAGGACTATTTTCAGGCTGGTGATTTATTGGCTATAAGGGTTGTTGCAAAAAATCAGGTA
AAACCTGAAAAGGTTCAGGA

Product: NADPH-flavin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
METLEAIHTRRSIRKYTDRPVPRELVTELLRAAMSAPSAVNAQPWVFIVIDDRKILDEIPTFSPYAGMCREAPLAILVCG
DTTQEKAPGYWVQDCSAAIQNLLLAAHDAGLGAVWTGIFPMTDRVQGFRKAFGLPGHVFPLGLVPVGYPAEKPSPQDRYR
EEKVYNNRYGQKRE

Sequences:

>Translated_174_residues
METLEAIHTRRSIRKYTDRPVPRELVTELLRAAMSAPSAVNAQPWVFIVIDDRKILDEIPTFSPYAGMCREAPLAILVCG
DTTQEKAPGYWVQDCSAAIQNLLLAAHDAGLGAVWTGIFPMTDRVQGFRKAFGLPGHVFPLGLVPVGYPAEKPSPQDRYR
EEKVYNNRYGQKRE
>Mature_174_residues
METLEAIHTRRSIRKYTDRPVPRELVTELLRAAMSAPSAVNAQPWVFIVIDDRKILDEIPTFSPYAGMCREAPLAILVCG
DTTQEKAPGYWVQDCSAAIQNLLLAAHDAGLGAVWTGIFPMTDRVQGFRKAFGLPGHVFPLGLVPVGYPAEKPSPQDRYR
EEKVYNNRYGQKRE

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19486; Mature: 19486

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLEAIHTRRSIRKYTDRPVPRELVTELLRAAMSAPSAVNAQPWVFIVIDDRKILDEIP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHCC
TFSPYAGMCREAPLAILVCGDTTQEKAPGYWVQDCSAAIQNLLLAAHDAGLGAVWTGIFP
CCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
MTDRVQGFRKAFGLPGHVFPLGLVPVGYPAEKPSPQDRYREEKVYNNRYGQKRE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
METLEAIHTRRSIRKYTDRPVPRELVTELLRAAMSAPSAVNAQPWVFIVIDDRKILDEIP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHCC
TFSPYAGMCREAPLAILVCGDTTQEKAPGYWVQDCSAAIQNLLLAAHDAGLGAVWTGIFP
CCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
MTDRVQGFRKAFGLPGHVFPLGLVPVGYPAEKPSPQDRYREEKVYNNRYGQKRE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]