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The map label for this gene is nifS [H]

Identifier: 21227619

GI number: 21227619

Start: 1808372

End: 1809553

Strand: Reverse

Name: nifS [H]

Synonym: MM_1517

Alternate gene names: 21227619

Gene position: 1809553-1808372 (Counterclockwise)

Preceding gene: 21227620

Following gene: 21227618

Centisome position: 44.17

GC content: 48.82

Gene sequence:

>1182_bases
ATGTACGATGTAGATGCGGTCCGTGAAGATTTTCCTGTTTTAAAAGAAGTTGTGTACCTTGACAGTACGGCGACCACGCA
GACTCCGGTCCCTGCAGTCGAAGCCATGGTTGAATATTTCTACAGGTATGCCGGCAACCACGGGAGAGGAGCGCACCGCC
TTGCCAGAGAGACCACAAACCGGTACGAAGACGCAAGGGAAACTGTTGCCCATTTTCTTAATGCAGACCCTTCAAAGACC
GTATTTACGAAAAACACGACCGAAGGGATAAACCTTATTGCAAATAGCTATCCCTGGGAGGCAGGAGACCATATAGTTAC
AACCATGCTCGAGCATCATTCAAACCTTCTTCCCTGGCTGCGCCTGCAAAAAAAAGGAATTGATGTGACAGTGATAAAAC
CGGACCGTGAAGGCATAATTGATCCCCGCATGATAGAAAATGCCCTTACTGACAGGACGAAACTGATTGCCGTCACGCAT
ATCTCAAACGTTTTCGGGTCCGTCCAGAACGTCAGCAGGATCACGAAACTTGCCCGCAAAAACGGAGTAAAAATCCTCGT
TGACGGGGCTCAGTCCGCAGGGCATGTACCGGTGAACATTAAAACACTTGGATGTGACTTTTTTGCAACCGCAGGGCATA
AAGGGCTTCTAGGACCCCAGGGGACTGGCGTACTTTACATTAAAGATCCTGAAGTGATGGAAAGCGCCTCTCTGGGAGGA
GGAACGGTTTCGGATGTCAGAGGGCATGAGTACACTCTTGAGCCCTCCCCGACATGTTTTGAAGCCGGCACCCCGAATAT
CCCGGGGGTTATAGGGCTTGGAAAGGCAGTAGAGTATGTAAAAAAAATCGGAATTTCCGAAATTGAAAAGCATGAAGCTG
AGCTTTCCCGGCAGGCTGCGAAAAGGCTCTCGGAACTTGATCACGTAGAGGTTTACGGGCCTGAAAACAGGGCAGGGATT
GTTCCTTTTAATGTTACCGGGCTTCATGCCCATGATGTCGCCCTGATTCTTGACCAGACAAGAAAAATTTGCGTCCGTAG
CGGACACCATTGTGCAATCCCTGTTACCCGTTTCCTCGAAGTGGAAAGTACGGTAAGGGCTTCTTTTGCACTTTATAACA
CAAAAGAAGAGGTAGATATTCTTGTAAAGGCTGTGGATGCTCTCAAGGCTCTCGTATCCTGA

Upstream 100 bases:

>100_bases
TGATACAGATGAATTAGACCTTTACCGCTATATGATAGGCGGGATGCAGGGTAAATAACCTGCACAACCTTTTTTTATTT
CTCGTTCCTAGAAAAGAAGC

Downstream 100 bases:

>100_bases
AAACCTGAATCTCTACCCGGGCAGAAGAAAAATTATATTGCAGCCTGGAGATTTTTTCTGGCACACACTTTATATTTTTA
CAGATTAATTTTAATACAGG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MYDVDAVREDFPVLKEVVYLDSTATTQTPVPAVEAMVEYFYRYAGNHGRGAHRLARETTNRYEDARETVAHFLNADPSKT
VFTKNTTEGINLIANSYPWEAGDHIVTTMLEHHSNLLPWLRLQKKGIDVTVIKPDREGIIDPRMIENALTDRTKLIAVTH
ISNVFGSVQNVSRITKLARKNGVKILVDGAQSAGHVPVNIKTLGCDFFATAGHKGLLGPQGTGVLYIKDPEVMESASLGG
GTVSDVRGHEYTLEPSPTCFEAGTPNIPGVIGLGKAVEYVKKIGISEIEKHEAELSRQAAKRLSELDHVEVYGPENRAGI
VPFNVTGLHAHDVALILDQTRKICVRSGHHCAIPVTRFLEVESTVRASFALYNTKEEVDILVKAVDALKALVS

Sequences:

>Translated_393_residues
MYDVDAVREDFPVLKEVVYLDSTATTQTPVPAVEAMVEYFYRYAGNHGRGAHRLARETTNRYEDARETVAHFLNADPSKT
VFTKNTTEGINLIANSYPWEAGDHIVTTMLEHHSNLLPWLRLQKKGIDVTVIKPDREGIIDPRMIENALTDRTKLIAVTH
ISNVFGSVQNVSRITKLARKNGVKILVDGAQSAGHVPVNIKTLGCDFFATAGHKGLLGPQGTGVLYIKDPEVMESASLGG
GTVSDVRGHEYTLEPSPTCFEAGTPNIPGVIGLGKAVEYVKKIGISEIEKHEAELSRQAAKRLSELDHVEVYGPENRAGI
VPFNVTGLHAHDVALILDQTRKICVRSGHHCAIPVTRFLEVESTVRASFALYNTKEEVDILVKAVDALKALVS
>Mature_393_residues
MYDVDAVREDFPVLKEVVYLDSTATTQTPVPAVEAMVEYFYRYAGNHGRGAHRLARETTNRYEDARETVAHFLNADPSKT
VFTKNTTEGINLIANSYPWEAGDHIVTTMLEHHSNLLPWLRLQKKGIDVTVIKPDREGIIDPRMIENALTDRTKLIAVTH
ISNVFGSVQNVSRITKLARKNGVKILVDGAQSAGHVPVNIKTLGCDFFATAGHKGLLGPQGTGVLYIKDPEVMESASLGG
GTVSDVRGHEYTLEPSPTCFEAGTPNIPGVIGLGKAVEYVKKIGISEIEKHEAELSRQAAKRLSELDHVEVYGPENRAGI
VPFNVTGLHAHDVALILDQTRKICVRSGHHCAIPVTRFLEVESTVRASFALYNTKEEVDILVKAVDALKALVS

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=413, Percent_Identity=27.3607748184019, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI156713448, Length=424, Percent_Identity=25.4716981132075, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1787970, Length=410, Percent_Identity=40.9756097560976, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=39.4936708860759, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI48994898, Length=389, Percent_Identity=28.7917737789203, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25143064, Length=395, Percent_Identity=27.8481012658228, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI193211090, Length=377, Percent_Identity=24.9336870026525, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17533177, Length=218, Percent_Identity=31.651376146789, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319831, Length=392, Percent_Identity=30.1020408163265, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI20129463, Length=266, Percent_Identity=32.3308270676692, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43065; Mature: 43065

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVDAVREDFPVLKEVVYLDSTATTQTPVPAVEAMVEYFYRYAGNHGRGAHRLARETTN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RYEDARETVAHFLNADPSKTVFTKNTTEGINLIANSYPWEAGDHIVTTMLEHHSNLLPWL
HHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHEECCCCCCCCCHHHHHHHHHHCCCCHHH
RLQKKGIDVTVIKPDREGIIDPRMIENALTDRTKLIAVTHISNVFGSVQNVSRITKLARK
HHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
NGVKILVDGAQSAGHVPVNIKTLGCDFFATAGHKGLLGPQGTGVLYIKDPEVMESASLGG
CCCEEEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCEEEEECCCHHHHHCCCCC
GTVSDVRGHEYTLEPSPTCFEAGTPNIPGVIGLGKAVEYVKKIGISEIEKHEAELSRQAA
CCHHCCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KRLSELDHVEVYGPENRAGIVPFNVTGLHAHDVALILDQTRKICVRSGHHCAIPVTRFLE
HHHHHCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECHHHHHH
VESTVRASFALYNTKEEVDILVKAVDALKALVS
HHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MYDVDAVREDFPVLKEVVYLDSTATTQTPVPAVEAMVEYFYRYAGNHGRGAHRLARETTN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RYEDARETVAHFLNADPSKTVFTKNTTEGINLIANSYPWEAGDHIVTTMLEHHSNLLPWL
HHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHEECCCCCCCCCHHHHHHHHHHCCCCHHH
RLQKKGIDVTVIKPDREGIIDPRMIENALTDRTKLIAVTHISNVFGSVQNVSRITKLARK
HHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
NGVKILVDGAQSAGHVPVNIKTLGCDFFATAGHKGLLGPQGTGVLYIKDPEVMESASLGG
CCCEEEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCEEEEECCCHHHHHCCCCC
GTVSDVRGHEYTLEPSPTCFEAGTPNIPGVIGLGKAVEYVKKIGISEIEKHEAELSRQAA
CCHHCCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KRLSELDHVEVYGPENRAGIVPFNVTGLHAHDVALILDQTRKICVRSGHHCAIPVTRFLE
HHHHHCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECHHHHHH
VESTVRASFALYNTKEEVDILVKAVDALKALVS
HHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]