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The map label for this gene is moaA

Identifier: 21227495

GI number: 21227495

Start: 1676170

End: 1677174

Strand: Reverse

Name: moaA

Synonym: MM_1393

Alternate gene names: 21227495

Gene position: 1677174-1676170 (Counterclockwise)

Preceding gene: 21227498

Following gene: 21227494

Centisome position: 40.94

GC content: 45.47

Gene sequence:

>1005_bases
ATGAAGGACAACAATTCAGGGAAAAATTCCCTGTTTCGGGAAGAAAACGAAGAAGAAGTCCTGGTTGACCCTTACGGGCG
CAAAGTAACCGGGCTCAGGATATCGATTACTGACAGGTGTAACCTTTCCTGTATGTACTGCCATAACGAAGGAGCAGAAT
GCTGTACCTGCGGTCCTGTAGGGAATGAAATGAGTCCGGAACTGATTTGCAGCATAATCAGAGAAGCAGCAAAATTTGGA
GTCCGTAAAGTAAAATTTTCAGGAGGAGAACCTCTTTTCAGAAAGGATTTTGAAGATATCCTTGCCTGCCTGCCTCCTCT
GAAAGAGGTTTCGGCAACCACTAACGGCATCCTGCTTGAAAAACGTGCAAAAACCCTGAAAGCTGCCGGACTCGACAGGA
TAAACGTAAGCCTGGACTCTCTTGACCCCGAAAAATACAGGAAGATTACCGGAGCCCCTCCGGGAACCCTTGAGAAGGTC
ATCAGAGGTATCAACAGCGCAGTTGAAGCAGGGTTAACTCCGGTAAAACTGAATATGGTACTCCTGAAGGGCATAAACGA
GAACGAAATCGATGAAATGATGGATTTCATCCGCCCTTATAAAGGAAAAGTCATCCTGCAGCTTATTGAGCTCATGAATA
TAGACCCTGAGCTTTCAAAGTACACTATCGACTCAAAAACCCTTGAAAAAAGCCTCGAAGAAAGAGCAAGCGAAGTCAGG
GTCCGGCACCTGCACCACAGAAAAAAATACATGATCGATGGAGTTGAGGTGGAATTCGTGCGCCCGATGGATAATTCTGA
GTTCTGCGCCCACTGCAGCAGGCTTAGAGTCACAGCCGATGGGAAACTCAGGCCCTGCCTGCTTGTGCACGATAATCTTG
TTGATATAGGAGGAGCAAATAGTCCTGAAGAGATAGAAAAACTGCTCAGGCTTGCAGTAAGCCGGAGAAAGCCTTATTAT
ACTCCAATCATGAAAATTGAGAAATTAAAGAAAAAGAAAGAATAA

Upstream 100 bases:

>100_bases
TATTCTTTTAGTTACAGAGCTTTTAATTACTCCGGTTAAACGGACCATAACTCTACCAGAAAATATTATTATCTCTATAA
TATAAATACGTCAGGGAAAT

Downstream 100 bases:

>100_bases
ATAAAAGGATCAAAGAAAAAAAGAATTAAAGAAAGAAGAAAAGAGAAAAAATATTCAAGGAGAGATAAAATGAAAATCGA
ACTTCTGGTAGACGGCAGAA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKDNNSGKNSLFREENEEEVLVDPYGRKVTGLRISITDRCNLSCMYCHNEGAECCTCGPVGNEMSPELICSIIREAAKFG
VRKVKFSGGEPLFRKDFEDILACLPPLKEVSATTNGILLEKRAKTLKAAGLDRINVSLDSLDPEKYRKITGAPPGTLEKV
IRGINSAVEAGLTPVKLNMVLLKGINENEIDEMMDFIRPYKGKVILQLIELMNIDPELSKYTIDSKTLEKSLEERASEVR
VRHLHHRKKYMIDGVEVEFVRPMDNSEFCAHCSRLRVTADGKLRPCLLVHDNLVDIGGANSPEEIEKLLRLAVSRRKPYY
TPIMKIEKLKKKKE

Sequences:

>Translated_334_residues
MKDNNSGKNSLFREENEEEVLVDPYGRKVTGLRISITDRCNLSCMYCHNEGAECCTCGPVGNEMSPELICSIIREAAKFG
VRKVKFSGGEPLFRKDFEDILACLPPLKEVSATTNGILLEKRAKTLKAAGLDRINVSLDSLDPEKYRKITGAPPGTLEKV
IRGINSAVEAGLTPVKLNMVLLKGINENEIDEMMDFIRPYKGKVILQLIELMNIDPELSKYTIDSKTLEKSLEERASEVR
VRHLHHRKKYMIDGVEVEFVRPMDNSEFCAHCSRLRVTADGKLRPCLLVHDNLVDIGGANSPEEIEKLLRLAVSRRKPYY
TPIMKIEKLKKKKE
>Mature_334_residues
MKDNNSGKNSLFREENEEEVLVDPYGRKVTGLRISITDRCNLSCMYCHNEGAECCTCGPVGNEMSPELICSIIREAAKFG
VRKVKFSGGEPLFRKDFEDILACLPPLKEVSATTNGILLEKRAKTLKAAGLDRINVSLDSLDPEKYRKITGAPPGTLEKV
IRGINSAVEAGLTPVKLNMVLLKGINENEIDEMMDFIRPYKGKVILQLIELMNIDPELSKYTIDSKTLEKSLEERASEVR
VRHLHHRKKYMIDGVEVEFVRPMDNSEFCAHCSRLRVTADGKLRPCLLVHDNLVDIGGANSPEEIEKLLRLAVSRRKPYY
TPIMKIEKLKKKKE

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=334, Percent_Identity=33.8323353293413, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI22027536, Length=334, Percent_Identity=33.8323353293413, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1786999, Length=286, Percent_Identity=34.965034965035, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17561210, Length=330, Percent_Identity=34.5454545454545, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566329, Length=327, Percent_Identity=33.9449541284404, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566331, Length=318, Percent_Identity=34.5911949685535, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28574488, Length=328, Percent_Identity=33.2317073170732, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28574490, Length=316, Percent_Identity=34.4936708860759, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_METMA (Q8PX29)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633417.1
- ProteinModelPortal:   Q8PX29
- SMR:   Q8PX29
- GeneID:   1479735
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1393
- NMPDR:   fig|192952.1.peg.1393
- HOGENOM:   HBG529885
- OMA:   IGEASGW
- ProtClustDB:   PRK00164
- BioCyc:   MMAZ192952:MM1393-MONOMER
- HAMAP:   MF_01225_A
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013485
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02668

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37691; Mature: 37691

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 33-33 BINDING 46-46 BINDING 85-85 BINDING 89-89 BINDING 113-113 BINDING 137-137 BINDING 176-176 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNNSGKNSLFREENEEEVLVDPYGRKVTGLRISITDRCNLSCMYCHNEGAECCTCGPV
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCCCEEEEEEECCCCCEEECCCC
GNEMSPELICSIIREAAKFGVRKVKFSGGEPLFRKDFEDILACLPPLKEVSATTNGILLE
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCCEEEH
KRAKTLKAAGLDRINVSLDSLDPEKYRKITGAPPGTLEKVIRGINSAVEAGLTPVKLNMV
HHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
LLKGINENEIDEMMDFIRPYKGKVILQLIELMNIDPELSKYTIDSKTLEKSLEERASEVR
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHEECCHHHHHHHHHHHHHHHH
VRHLHHRKKYMIDGVEVEFVRPMDNSEFCAHCSRLRVTADGKLRPCLLVHDNLVDIGGAN
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHEEEEECCCCCCEEEEECCEEECCCCC
SPEEIEKLLRLAVSRRKPYYTPIMKIEKLKKKKE
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKDNNSGKNSLFREENEEEVLVDPYGRKVTGLRISITDRCNLSCMYCHNEGAECCTCGPV
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCCCEEEEEEECCCCCEEECCCC
GNEMSPELICSIIREAAKFGVRKVKFSGGEPLFRKDFEDILACLPPLKEVSATTNGILLE
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCCEEEH
KRAKTLKAAGLDRINVSLDSLDPEKYRKITGAPPGTLEKVIRGINSAVEAGLTPVKLNMV
HHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
LLKGINENEIDEMMDFIRPYKGKVILQLIELMNIDPELSKYTIDSKTLEKSLEERASEVR
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHEECCHHHHHHHHHHHHHHHH
VRHLHHRKKYMIDGVEVEFVRPMDNSEFCAHCSRLRVTADGKLRPCLLVHDNLVDIGGAN
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHEEEEECCCCCCEEEEECCEEECCCCC
SPEEIEKLLRLAVSRRKPYYTPIMKIEKLKKKKE
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824