Click here to switch to the map view.

The map label for this gene is hycG [C]

Identifier: 21227166

GI number: 21227166

Start: 1249083

End: 1249595

Strand: Direct

Name: hycG [C]

Synonym: MM_1064

Alternate gene names: 21227166

Gene position: 1249083-1249595 (Clockwise)

Preceding gene: 21227165

Following gene: 21227167

Centisome position: 30.49

GC content: 47.17

Gene sequence:

>513_bases
ATGGTAAACCCTCTTTCCCTTTTCCTTCGTCCGAAAGTTACGGAGACATTTGAGTTCAGGGACGAAGAACTGGAAGCCCT
TGGAGCACAAATAAAAAAAGAAATTCCTAAAGTTTTCGGGCACAGCCTTGCAATCCGTGAGCTGGACTCGGGAAGCGACA
ATTCTACGGAGATGGAAATTTCTAACCTTGGAAACCCGCATTATGATGTTGAGAGATTCGGGATTTCTTTTGTAGCGTCT
CCCAGGCATGCTGATGTCCTTCTGGTTACAGGGGCAGTCACCCTTAATATGGCTGAAGCCGCAAAAAAGACCTATGAAGC
CATGCCTTACCCCAAGTATGTAATAGCAGTCGGAGACGACGCCTGTGACGGCGGGATCTACAAGGGTTCTTACGCCGTAC
TTGGGGGAGTTGACAAAATCCTTCCCGTGGATGTAAAAATTCCAGGGAACCCGCCCTCTCCAAAAGAAATTCTTCAGGGA
ATTTTTGCCCTTGTAAAAAAAGCCCGGGAATAA

Upstream 100 bases:

>100_bases
GGAACGGACCTTTGAAAAGAGAAAAAGGCAGTTGAAGGTTCAAGCAAAAAAACAGTGAACAGATTCAATTAAAGAATATA
AAGAAAAAGTGAGGTCTTAT

Downstream 100 bases:

>100_bases
TCCGGGGATCTCTCCTGCCTAATGCAGGAGTCAACTTCTTTTTACTTCTGGTTATATTCCTTTGATCAGAACCCGTTTAC
ATCATACCCGTTTACTGAAG

Product: formate hydrogenlyase subunit 7

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MVNPLSLFLRPKVTETFEFRDEELEALGAQIKKEIPKVFGHSLAIRELDSGSDNSTEMEISNLGNPHYDVERFGISFVAS
PRHADVLLVTGAVTLNMAEAAKKTYEAMPYPKYVIAVGDDACDGGIYKGSYAVLGGVDKILPVDVKIPGNPPSPKEILQG
IFALVKKARE

Sequences:

>Translated_170_residues
MVNPLSLFLRPKVTETFEFRDEELEALGAQIKKEIPKVFGHSLAIRELDSGSDNSTEMEISNLGNPHYDVERFGISFVAS
PRHADVLLVTGAVTLNMAEAAKKTYEAMPYPKYVIAVGDDACDGGIYKGSYAVLGGVDKILPVDVKIPGNPPSPKEILQG
IFALVKKARE
>Mature_170_residues
MVNPLSLFLRPKVTETFEFRDEELEALGAQIKKEIPKVFGHSLAIRELDSGSDNSTEMEISNLGNPHYDVERFGISFVAS
PRHADVLLVTGAVTLNMAEAAKKTYEAMPYPKYVIAVGDDACDGGIYKGSYAVLGGVDKILPVDVKIPGNPPSPKEILQG
IFALVKKARE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=116, Percent_Identity=43.9655172413793, Blast_Score=115, Evalue=2e-26,
Organism=Escherichia coli, GI1789074, Length=144, Percent_Identity=44.4444444444444, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI1788834, Length=140, Percent_Identity=40.7142857142857, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788624, Length=98, Percent_Identity=44.8979591836735, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=116, Percent_Identity=41.3793103448276, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24651058, Length=179, Percent_Identity=35.195530726257, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859983, Length=116, Percent_Identity=42.2413793103448, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24642371, Length=116, Percent_Identity=42.2413793103448, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 18506; Mature: 18506

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNPLSLFLRPKVTETFEFRDEELEALGAQIKKEIPKVFGHSLAIRELDSGSDNSTEMEI
CCCCHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEH
SNLGNPHYDVERFGISFVASPRHADVLLVTGAVTLNMAEAAKKTYEAMPYPKYVIAVGDD
HHCCCCCCCHHHHCEEEEECCCCCCEEEEECEEEEHHHHHHHHHHHCCCCCCEEEEECCC
ACDGGIYKGSYAVLGGVDKILPVDVKIPGNPPSPKEILQGIFALVKKARE
CCCCCEECCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVNPLSLFLRPKVTETFEFRDEELEALGAQIKKEIPKVFGHSLAIRELDSGSDNSTEMEI
CCCCHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEH
SNLGNPHYDVERFGISFVASPRHADVLLVTGAVTLNMAEAAKKTYEAMPYPKYVIAVGDD
HHCCCCCCCHHHHCEEEEECCCCCCEEEEECEEEEHHHHHHHHHHHCCCCCCEEEEECCC
ACDGGIYKGSYAVLGGVDKILPVDVKIPGNPPSPKEILQGIFALVKKARE
CCCCCEECCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]