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The map label for this gene is glnA [H]

Identifier: 21227066

GI number: 21227066

Start: 1142131

End: 1143474

Strand: Direct

Name: glnA [H]

Synonym: MM_0964

Alternate gene names: 21227066

Gene position: 1142131-1143474 (Clockwise)

Preceding gene: 21227064

Following gene: 21227067

Centisome position: 27.88

GC content: 46.73

Gene sequence:

>1344_bases
ATGGTGCAGATGAAAAAGTGTACAACTAAAGAAGATGTGTTAGAGGCCGTAAAAGAACGAGACGTGAAGTTTATCCGTAC
CCAGTTTACGGATACACTCGGCATCATCAAAAGCTGGGCAATTCCTGCGGAACAACTGGAAGAAGCTTTTGAAAACGGTG
TAATGTTTGACGGATCTTCAATCCAGGGTTTTACAAGGATTGAAGAATCTGATATGAAACTCGCACTTGACCCTTCAACA
TTCAGAATCCTGCCCTGGAGACCTGCAACCGGCGCAGTAGCCAGGATTCTCGGAGATGTATATCTTCCTGACGGAAACCC
CTTCAAGGGAGACCCGAGATATGTACTTAAGACCGCAATCAAAGAAGCCGAAAAAATGGGCTTCTCAATGAACGTAGGGC
CGGAACTTGAGTTTTTCCTGTTCAAGCTTGATGCAAATGGAAACCCGACAACCGAACTTACCGACCAGGGAGGATACTTC
GATTTCGCACCCCTCGACCGTGCACAGGATGTCCGCAGAGACATTGACTACGCCCTGGAACACATGGGCTTCCAGATAGA
AGCCTCCCATCACGAAGTTGCACCTTCCCAGCATGAAATAGACTTCAGGTTCGGAGACGTGCTTTGCACAGCAGACAATG
TTGTGACTTTCAAATACGTGGTAAAATCAATTGCTTACCACAAAGGCTACTATGCCTCCTTCATGCCAAAGCCTCTCTTT
GGTGTAAACGGATCAGGTATGCACAGCAACCAGTCCCTTTTTAAGGATGGAAAGAACGTTTTCTATGACCCGGACACTCC
AACTAAACTGTCCCAGGATGCAATGTATTATATAGGCGGGCTGTTGAAGCACATCAGAGAATTTACAGCTGTTACGAACC
CTGTAGTAAACTCTTACAAGAGACTTGTCCCCGGCTATGAAGCTCCTGTTTATATTTCATGGTCTGCACAAAACAGAAGT
TCTCTGATCCGTATCCCGGCAACCAGAGGAAACGGAACAAGAATAGAACTCAGATGTCCGGACCCGGCATGCAACCCTTA
CCTTGCATTTGCTCTCATGCTCAGAGCTGGCCTTGAGGGGATAAAGAACAAAATTGATCCCGGAGAGCCTACCAACGTAA
ACATATTCCACCTTTCGGACAAAGAACGCGAAGAGAGAGGAATCCGTTCCCTTCCCGCAGACCTTAAGGAAGCAATCGAT
GAAATGAAAGGCAGCAAGTTCGTAAAGGAAGCCCTCGGAGAACATGTTTTCAGCCATTACCTCTGTGCAAAAGAGATGGA
ATGGGACGAATACAAAGCAGTTGTCCACCCCTGGGAACTTTCCAGATATCTCTCTATGCTGTAA

Upstream 100 bases:

>100_bases
ACCGGGTTTCTAAAGTATCTTAGATCCGGGATTTGTAGTAAATTATATATAGTATCAGATAGGTAAATGGTTACCTAATG
CCTAGTGAGGATGGAATCAA

Downstream 100 bases:

>100_bases
ATATATAGAAGAAAGATTTGACTTAAGATGTGCCGGAAGGATCTGCTCCAGGCCTTAAAAACGTTTATTAAGGGGTATAC
TGCCCATTAAGGGATATGTT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MVQMKKCTTKEDVLEAVKERDVKFIRTQFTDTLGIIKSWAIPAEQLEEAFENGVMFDGSSIQGFTRIEESDMKLALDPST
FRILPWRPATGAVARILGDVYLPDGNPFKGDPRYVLKTAIKEAEKMGFSMNVGPELEFFLFKLDANGNPTTELTDQGGYF
DFAPLDRAQDVRRDIDYALEHMGFQIEASHHEVAPSQHEIDFRFGDVLCTADNVVTFKYVVKSIAYHKGYYASFMPKPLF
GVNGSGMHSNQSLFKDGKNVFYDPDTPTKLSQDAMYYIGGLLKHIREFTAVTNPVVNSYKRLVPGYEAPVYISWSAQNRS
SLIRIPATRGNGTRIELRCPDPACNPYLAFALMLRAGLEGIKNKIDPGEPTNVNIFHLSDKEREERGIRSLPADLKEAID
EMKGSKFVKEALGEHVFSHYLCAKEMEWDEYKAVVHPWELSRYLSML

Sequences:

>Translated_447_residues
MVQMKKCTTKEDVLEAVKERDVKFIRTQFTDTLGIIKSWAIPAEQLEEAFENGVMFDGSSIQGFTRIEESDMKLALDPST
FRILPWRPATGAVARILGDVYLPDGNPFKGDPRYVLKTAIKEAEKMGFSMNVGPELEFFLFKLDANGNPTTELTDQGGYF
DFAPLDRAQDVRRDIDYALEHMGFQIEASHHEVAPSQHEIDFRFGDVLCTADNVVTFKYVVKSIAYHKGYYASFMPKPLF
GVNGSGMHSNQSLFKDGKNVFYDPDTPTKLSQDAMYYIGGLLKHIREFTAVTNPVVNSYKRLVPGYEAPVYISWSAQNRS
SLIRIPATRGNGTRIELRCPDPACNPYLAFALMLRAGLEGIKNKIDPGEPTNVNIFHLSDKEREERGIRSLPADLKEAID
EMKGSKFVKEALGEHVFSHYLCAKEMEWDEYKAVVHPWELSRYLSML
>Mature_447_residues
MVQMKKCTTKEDVLEAVKERDVKFIRTQFTDTLGIIKSWAIPAEQLEEAFENGVMFDGSSIQGFTRIEESDMKLALDPST
FRILPWRPATGAVARILGDVYLPDGNPFKGDPRYVLKTAIKEAEKMGFSMNVGPELEFFLFKLDANGNPTTELTDQGGYF
DFAPLDRAQDVRRDIDYALEHMGFQIEASHHEVAPSQHEIDFRFGDVLCTADNVVTFKYVVKSIAYHKGYYASFMPKPLF
GVNGSGMHSNQSLFKDGKNVFYDPDTPTKLSQDAMYYIGGLLKHIREFTAVTNPVVNSYKRLVPGYEAPVYISWSAQNRS
SLIRIPATRGNGTRIELRCPDPACNPYLAFALMLRAGLEGIKNKIDPGEPTNVNIFHLSDKEREERGIRSLPADLKEAID
EMKGSKFVKEALGEHVFSHYLCAKEMEWDEYKAVVHPWELSRYLSML

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=440, Percent_Identity=25.6818181818182, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790301, Length=478, Percent_Identity=41.0041841004184, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI87081870, Length=446, Percent_Identity=26.6816143497758, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50593; Mature: 50593

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQMKKCTTKEDVLEAVKERDVKFIRTQFTDTLGIIKSWAIPAEQLEEAFENGVMFDGSS
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCC
IQGFTRIEESDMKLALDPSTFRILPWRPATGAVARILGDVYLPDGNPFKGDPRYVLKTAI
CCCHHEECCCCCEEEECCCCEEEEECCCCHHHHHHHHCCEECCCCCCCCCCHHHHHHHHH
KEAEKMGFSMNVGPELEFFLFKLDANGNPTTELTDQGGYFDFAPLDRAQDVRRDIDYALE
HHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEECCCCCEECCCCCCHHHHHHHHHHHHHH
HMGFQIEASHHEVAPSQHEIDFRFGDVLCTADNVVTFKYVVKSIAYHKGYYASFMPKPLF
HCCEEEECCCCCCCCCCCCEEEEECCEEEECCCEEHHHHHHHHHHHHCCCHHHCCCCCCC
GVNGSGMHSNQSLFKDGKNVFYDPDTPTKLSQDAMYYIGGLLKHIREFTAVTNPVVNSYK
CCCCCCCCCCHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLVPGYEAPVYISWSAQNRSSLIRIPATRGNGTRIELRCPDPACNPYLAFALMLRAGLEG
HHCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
IKNKIDPGEPTNVNIFHLSDKEREERGIRSLPADLKEAIDEMKGSKFVKEALGEHVFSHY
HHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LCAKEMEWDEYKAVVHPWELSRYLSML
HHHHCCCHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MVQMKKCTTKEDVLEAVKERDVKFIRTQFTDTLGIIKSWAIPAEQLEEAFENGVMFDGSS
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCC
IQGFTRIEESDMKLALDPSTFRILPWRPATGAVARILGDVYLPDGNPFKGDPRYVLKTAI
CCCHHEECCCCCEEEECCCCEEEEECCCCHHHHHHHHCCEECCCCCCCCCCHHHHHHHHH
KEAEKMGFSMNVGPELEFFLFKLDANGNPTTELTDQGGYFDFAPLDRAQDVRRDIDYALE
HHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEECCCCCEECCCCCCHHHHHHHHHHHHHH
HMGFQIEASHHEVAPSQHEIDFRFGDVLCTADNVVTFKYVVKSIAYHKGYYASFMPKPLF
HCCEEEECCCCCCCCCCCCEEEEECCEEEECCCEEHHHHHHHHHHHHCCCHHHCCCCCCC
GVNGSGMHSNQSLFKDGKNVFYDPDTPTKLSQDAMYYIGGLLKHIREFTAVTNPVVNSYK
CCCCCCCCCCHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLVPGYEAPVYISWSAQNRSSLIRIPATRGNGTRIELRCPDPACNPYLAFALMLRAGLEG
HHCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
IKNKIDPGEPTNVNIFHLSDKEREERGIRSLPADLKEAIDEMKGSKFVKEALGEHVFSHY
HHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LCAKEMEWDEYKAVVHPWELSRYLSML
HHHHCCCHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]