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The map label for this gene is pycA [H]

Identifier: 161485671

GI number: 161485671

Start: 2179050

End: 2180531

Strand: Direct

Name: pycA [H]

Synonym: MM_1828

Alternate gene names: 161485671

Gene position: 2179050-2180531 (Clockwise)

Preceding gene: 21227929

Following gene: 21227931

Centisome position: 53.19

GC content: 48.72

Gene sequence:

>1482_bases
ATGTTCAAAAAAGTACTCGTTGCAAACCGCGGTGAAATAGCAATCAGGGTTATGCGTGCCTGCAGGGAGCTCGGGATTTC
AACAGTTGCTGTCTGTTCCGAGGCTGACAAAAGCGCCCTTTTTGCCAAGTATGCTGATGAAGCCTATCTGATAGGGCCTG
CCCCTTCAAGCCAGAGCTACCTGAATATGGAAGCTATCATTGAGGTTGCAAAAAACACCGGAGCTGAGGCAATCCACCCC
GGCTACGGTTTTCTTTCCGAAAACCCTGCTTTTGCAAAGCGCTGTGAGGAAGAAGGCATTATTTTCATAGGCCCTCCAAG
CAATGTAATTGCCGAAATGGGAAGCAAAATCAGAGCAAGGCACCTCATGATGAAAGCCGGAGTCCCTGTAGTTCCCGGAA
CAAAGGACGCAGTTGAGGACCCTGAAGAAGCCATGGAAATTGCAGAAAAGATCGGCTATCCTGTCCTTATCAAAGCGTCT
GCCGGAGGCGGTGGAATAGGGATGAAGGTTGTCCACTCCAGGGAAGAGCTTGCCACATCCTTAAGCTCTACAAGGCAGAT
GGCAGGTTCCGCTTTCGGGGATTCTTCCGTATTTATCGAAAAGTACGTGGAAGAGCCAAGGCATATAGAGATCCAGATCC
TTGCTGACTCTCATGGTAATATGGTCTACCTCTCAGACAGGGAATGCTCAATCCAGAGAAGGCACCAGAAGCTGATTGAA
GAAGCTCCTTCCCCTATAATGACTCCGGAACTCAGGAAGCAGATGGGGGAGGCTGCCGTAAAGGTTGCAAAAGCAATTGG
TTATGTAAACGCAGGGACCGTCGAGTTCCTTTACTCTAAAGGGAATTTCTATTTCCTTGAAGTCAATACTCGCCTTCAGG
TGGAACATGGAATTACCGAAATGGTAACCGGAATTGATATTGTCAGGGAACAGATCAGGATCGCCTGGGGAGACAAACTC
GATTTTGAACAGGAAGATATAGTTATTGACGGGCATGCAATCGAATGCAGAATAAATGCCGAAGATCCCCTGAATGATTT
TGCTCCTTCCCCTGGAAAGATCCGGAGGTACCGTTCTGCAGGAGGGCCCGGAGTAAGGGTGGACAGCGGAGTTCATACAG
GCTATACAATCTCTCCTTATTATGACTCCATGATTTCCAAGCTCTGTGTCTGGTCCAGAAAAAGAGAAGAGGCTATCGCC
AGGATGGACAGGGCACTCTACGAGTATGTGGTCGTTGGAGTAAAAACCAATATCCCTTTCCATAAAGCTGTTATGAGGAA
CCCTGCATTCCGCAGAGGCGACATGACAACCAGCTTTATCGAAGAGGAGAATATCCTTGAGGCTGTGGAACACGTCGTTA
AGGCTGACAGTGAGAAAGGTGCTACTCTGGCTTCGGCCCTTGAGGCTAAGGACAAGAAAGTTGCAGCAATCACGGCCGCT
GTACATGCCTATGTTAACATGGCCCAGAAAACACAGCGGTGA

Upstream 100 bases:

>100_bases
TCGGGAATAGTTAAGGAAATCTTTGTAGCCGAAGGGGACACCGTCGCGCCCGGAGATATCATCCTTTCAATCGAGTAAAT
CGGGAAAGGTGGAAGGTATC

Downstream 100 bases:

>100_bases
TGCTTTTCACTCTTCGGAAAAGAGGAAAAAGATTTTCCGGCTTATCAGGGTATGACCATATGTCTGGACTAAGAGAATAT
ATACGGTGGAACATATACAT

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MFKKVLVANRGEIAIRVMRACRELGISTVAVCSEADKSALFAKYADEAYLIGPAPSSQSYLNMEAIIEVAKNTGAEAIHP
GYGFLSENPAFAKRCEEEGIIFIGPPSNVIAEMGSKIRARHLMMKAGVPVVPGTKDAVEDPEEAMEIAEKIGYPVLIKAS
AGGGGIGMKVVHSREELATSLSSTRQMAGSAFGDSSVFIEKYVEEPRHIEIQILADSHGNMVYLSDRECSIQRRHQKLIE
EAPSPIMTPELRKQMGEAAVKVAKAIGYVNAGTVEFLYSKGNFYFLEVNTRLQVEHGITEMVTGIDIVREQIRIAWGDKL
DFEQEDIVIDGHAIECRINAEDPLNDFAPSPGKIRRYRSAGGPGVRVDSGVHTGYTISPYYDSMISKLCVWSRKREEAIA
RMDRALYEYVVVGVKTNIPFHKAVMRNPAFRRGDMTTSFIEEENILEAVEHVVKADSEKGATLASALEAKDKKVAAITAA
VHAYVNMAQKTQR

Sequences:

>Translated_493_residues
MFKKVLVANRGEIAIRVMRACRELGISTVAVCSEADKSALFAKYADEAYLIGPAPSSQSYLNMEAIIEVAKNTGAEAIHP
GYGFLSENPAFAKRCEEEGIIFIGPPSNVIAEMGSKIRARHLMMKAGVPVVPGTKDAVEDPEEAMEIAEKIGYPVLIKAS
AGGGGIGMKVVHSREELATSLSSTRQMAGSAFGDSSVFIEKYVEEPRHIEIQILADSHGNMVYLSDRECSIQRRHQKLIE
EAPSPIMTPELRKQMGEAAVKVAKAIGYVNAGTVEFLYSKGNFYFLEVNTRLQVEHGITEMVTGIDIVREQIRIAWGDKL
DFEQEDIVIDGHAIECRINAEDPLNDFAPSPGKIRRYRSAGGPGVRVDSGVHTGYTISPYYDSMISKLCVWSRKREEAIA
RMDRALYEYVVVGVKTNIPFHKAVMRNPAFRRGDMTTSFIEEENILEAVEHVVKADSEKGATLASALEAKDKKVAAITAA
VHAYVNMAQKTQR
>Mature_493_residues
MFKKVLVANRGEIAIRVMRACRELGISTVAVCSEADKSALFAKYADEAYLIGPAPSSQSYLNMEAIIEVAKNTGAEAIHP
GYGFLSENPAFAKRCEEEGIIFIGPPSNVIAEMGSKIRARHLMMKAGVPVVPGTKDAVEDPEEAMEIAEKIGYPVLIKAS
AGGGGIGMKVVHSREELATSLSSTRQMAGSAFGDSSVFIEKYVEEPRHIEIQILADSHGNMVYLSDRECSIQRRHQKLIE
EAPSPIMTPELRKQMGEAAVKVAKAIGYVNAGTVEFLYSKGNFYFLEVNTRLQVEHGITEMVTGIDIVREQIRIAWGDKL
DFEQEDIVIDGHAIECRINAEDPLNDFAPSPGKIRRYRSAGGPGVRVDSGVHTGYTISPYYDSMISKLCVWSRKREEAIA
RMDRALYEYVVVGVKTNIPFHKAVMRNPAFRRGDMTTSFIEEENILEAVEHVVKADSEKGATLASALEAKDKKVAAITAA
VHAYVNMAQKTQR

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=443, Percent_Identity=52.1444695259594, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI189095269, Length=444, Percent_Identity=53.3783783783784, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI65506442, Length=444, Percent_Identity=53.3783783783784, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI295821183, Length=444, Percent_Identity=53.3783783783784, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI106049528, Length=446, Percent_Identity=49.3273542600897, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI106049295, Length=446, Percent_Identity=49.3273542600897, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI106049292, Length=446, Percent_Identity=49.3273542600897, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI38679960, Length=502, Percent_Identity=34.0637450199203, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI38679977, Length=502, Percent_Identity=34.0637450199203, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI38679967, Length=502, Percent_Identity=34.0637450199203, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI38679971, Length=502, Percent_Identity=34.0637450199203, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI38679974, Length=502, Percent_Identity=34.0637450199203, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI134142062, Length=509, Percent_Identity=33.2023575638507, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI18105007, Length=227, Percent_Identity=25.9911894273128, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789654, Length=444, Percent_Identity=54.2792792792793, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17567343, Length=444, Percent_Identity=49.3243243243243, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17562816, Length=447, Percent_Identity=48.993288590604, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71987519, Length=477, Percent_Identity=45.7023060796646, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI133931226, Length=449, Percent_Identity=32.5167037861915, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71997168, Length=505, Percent_Identity=30.4950495049505, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI71997163, Length=505, Percent_Identity=30.4950495049505, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI193204318, Length=339, Percent_Identity=23.598820058997, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319695, Length=449, Percent_Identity=48.1069042316258, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321376, Length=449, Percent_Identity=48.1069042316258, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=42.5339366515837, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324343, Length=558, Percent_Identity=30.4659498207885, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6323863, Length=502, Percent_Identity=31.0756972111554, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24651757, Length=444, Percent_Identity=49.3243243243243, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI281363050, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652224, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652222, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652220, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652218, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652212, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652210, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652214, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19921944, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24652216, Length=446, Percent_Identity=50, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651759, Length=408, Percent_Identity=48.7745098039216, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI161076409, Length=504, Percent_Identity=32.3412698412698, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI161076407, Length=504, Percent_Identity=32.3412698412698, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24586460, Length=504, Percent_Identity=32.3412698412698, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24586458, Length=504, Percent_Identity=32.3412698412698, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 54178; Mature: 54178

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKVLVANRGEIAIRVMRACRELGISTVAVCSEADKSALFAKYADEAYLIGPAPSSQSY
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHCCCEEEECCCCCCCCC
LNMEAIIEVAKNTGAEAIHPGYGFLSENPAFAKRCEEEGIIFIGPPSNVIAEMGSKIRAR
CCHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
HLMMKAGVPVVPGTKDAVEDPEEAMEIAEKIGYPVLIKASAGGGGIGMKVVHSREELATS
HHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEHHHHHHHHH
LSSTRQMAGSAFGDSSVFIEKYVEEPRHIEIQILADSHGNMVYLSDRECSIQRRHQKLIE
HHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
EAPSPIMTPELRKQMGEAAVKVAKAIGYVNAGTVEFLYSKGNFYFLEVNTRLQVEHGITE
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCEEEECCCHHH
MVTGIDIVREQIRIAWGDKLDFEQEDIVIDGHAIECRINAEDPLNDFAPSPGKIRRYRSA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEEEEEECCCCCHHHCCCCCHHHHHHHCC
GGPGVRVDSGVHTGYTISPYYDSMISKLCVWSRKREEAIARMDRALYEYVVVGVKTNIPF
CCCCCEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCH
HKAVMRNPAFRRGDMTTSFIEEENILEAVEHVVKADSEKGATLASALEAKDKKVAAITAA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
VHAYVNMAQKTQR
HHHHHHHHHHCCC
>Mature Secondary Structure
MFKKVLVANRGEIAIRVMRACRELGISTVAVCSEADKSALFAKYADEAYLIGPAPSSQSY
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHCCCEEEECCCCCCCCC
LNMEAIIEVAKNTGAEAIHPGYGFLSENPAFAKRCEEEGIIFIGPPSNVIAEMGSKIRAR
CCHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
HLMMKAGVPVVPGTKDAVEDPEEAMEIAEKIGYPVLIKASAGGGGIGMKVVHSREELATS
HHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEHHHHHHHHH
LSSTRQMAGSAFGDSSVFIEKYVEEPRHIEIQILADSHGNMVYLSDRECSIQRRHQKLIE
HHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
EAPSPIMTPELRKQMGEAAVKVAKAIGYVNAGTVEFLYSKGNFYFLEVNTRLQVEHGITE
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCEEEECCCHHH
MVTGIDIVREQIRIAWGDKLDFEQEDIVIDGHAIECRINAEDPLNDFAPSPGKIRRYRSA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEEEEEECCCCCHHHCCCCCHHHHHHHCC
GGPGVRVDSGVHTGYTISPYYDSMISKLCVWSRKREEAIARMDRALYEYVVVGVKTNIPF
CCCCCEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCH
HKAVMRNPAFRRGDMTTSFIEEENILEAVEHVVKADSEKGATLASALEAKDKKVAAITAA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
VHAYVNMAQKTQR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]